LOCUS       MR225313                8970 bp    DNA     circular  SYN 
DEFINITION  Flt4 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 4 (Flt4)
ACCESSION   NM_008029
VERSION     NM_008029.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5127..5218 
                     /label="Myc-DDK"  
     misc_feature    5248..5833 
                     /label="PolyA signal"  
     misc_feature    5305..5324 
                     /label="Restriction Enzyme"  
     misc_feature    6109..6781 
                     /label="ColE1"  
     misc_feature    7211..8005 
                     /label="Kan/Neo"  
     misc_feature    8040..8342 
                     /label="SV40 ori"  
     misc_feature    8428..8905 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5109..5115 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5109 
                     /label="Flt4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCCGGGC GCTGCGCTGA ACCTGCGCCT GTGGCTCTGC CTCGGACTCC 
     1081 TCCAAGGCCT GGCAAATGGT TACTCCATGA CCCCTCCAAC CCTGAACATC ACAGAGGATT 
     1141 CATATGTGAT TGACACCGGG GACAGCCTAT CCATATCCTG CAGGGGACAG CACCCCCTCG 
     1201 AGTGGACCTG GCCAGGGGCC CAGGAGGTAC TGACCACAGG TGGGAAGGAC AGTGAAGACA 
     1261 CACGGGTTGT GCATGACTGT GAAGGCACAG AAGCTAGGCC CTACTGCAAG GTGCTACTGC 
     1321 TGGCCCAGAC TCACGCCAAC AACACGGGCA GCTACCACTG CTACTACAAG TACATCAAGG 
     1381 CCCGGATTGA GGGCACCACA GCTGCCAGCA CCTATGTGTT TGTAAGAGAC TTTAAACACC 
     1441 CTTTCATCAA CAAACCTGAC ACGCTCCTGG TCAACAGGAA GGACTCGATG TGGGTGCCCT 
     1501 GCTTGGTGTC CATTCCCGGC CTCAACATCA CACTGCGCTC GCAAAGCTCA GCGCTGCACC 
     1561 CCGACGGGCA GGAGGTGCTG TGGGATGACC GCCGGGGCAT GCGGGTGCCC ACTCAACTGT 
     1621 TGCGCGATGC CCTGTACCTG CAGTGCGAGA CCACCTGGGG TGACCAGAAC TTCCTTTCCA 
     1681 ATCTCTTCGT CGTGCACATC ACAGGCAATG AGCTCTATGA CATCCAGCTG TACCCCAAGA 
     1741 AGTCAATGGA GCTGTTGGTT GGAGAGAAGC TGGTTTTGAA CTGTACAGTG TGGGCTGAGT 
     1801 TCGACTCAGG TGTCACCTTC GACTGGGATT ATCCAGGGAA GCAGGCAGAG CGGGCTAAGT 
     1861 GGGTACCTGA GCGGCGTTCC CAGCAGACCC ACACAGAACT CTCCAGCATC CTGACCATCC 
     1921 ACAATGTCAG CCAGAATGAC CTGGGCCCCT ATGTGTGTGA GGCCAACAAT GGGATTCAGC 
     1981 GGTTCCGGGA AAGCACAGAG GTCATTGTGC ACGAAAAGCC CTTCATCAGT GTCGAGTGGC 
     2041 TCAAAGGACC TGTCCTGGAG GCCACAGCCG GTGACGAGCT GGTGAAGCTA CCCGTGAAGC 
     2101 TGGCAGCTTA TCCCCCACCG GAGTTCCAAT GGTACAAGGA CAGAAAGGCA GTGACTGGGC 
     2161 GCCACAATCC CCATGCTCTG GTGCTCAAAG AGGTGACCGA GGCCAGCGCA GGGGTCTACA 
     2221 CTCTCGCCCT GTGGAACTCT GCAGCTGGTC TGAGGCAAAA CATCAGTCTG GAGCTGGTGG 
     2281 TGAATGTGCC TCCTCACATC CACGAAAAGG AAGCCTCTTC ACCCAGCATC TACTCCCGCC 
     2341 ACAGTCGCCA GACCCTCACC TGCACCGCCT ATGGAGTACC CCAACCCCTC AGTGTCCAGT 
     2401 GGCACTGGAG GCCCTGGACA CCCTGCAAGA CGTTTGCCCA GCGCAGCCTC CGGAGGCGGC 
     2461 AGCAGCGGGA TGGCATGCCA CAGTGCCGAG ACTGGAAGGA GGTGACCACT CAGGATGCTG 
     2521 TGAACCCCAT CGAGAGTCTG GACAGCTGGA CGGAGTTTGT GGAGGGGAAG AATAAGACGG 
     2581 TGAGCAAGCT GGTGATCCAG GATGCCAATG TGTCAGCCAT GTACAAGTGT GTGGTCGTCA 
     2641 ACAAAGTGGG CCAGGATGAG AGACTCATCT ACTTCTATGT GACCACCATC CCGGACGGTT 
     2701 TCAGTATCGA GTCGGAGCCT TCTGAGGATC CCTTAGAAGG CCAGTCCGTG CGCCTCAGCT 
     2761 GCCGGGCGGA CAACTACACG TACGAACATC TGCGCTGGTA CCGGCTCAAC CTCTCCACAC 
     2821 TGCACGATGC TCAAGGGAAC CCCCTATTGC TGGACTGCAA AAACGTGCAC CTGTTTGCCA 
     2881 CGCCCCTAGA GGCCAACCTA GAGGAGGCAG AGCCCGGGGC CCGCCACGCC ACCCTCAGTT 
     2941 TGAATATCCC CCGAGTGGCG CCCGAGGACG AGGGTGACTA CGTGTGTGAA GTGCAGGATA 
     3001 GGCGCAGCCA GGACAAGCAC TGCCACAAGA AGTACCTGTC CGTGCAGGCC CTGGAAGCTC 
     3061 CTCGGCTCAC GCAGAACTTG ACCGACCTCC TGGTGAACGT GAGTGACTCC CTGGAGATGC 
     3121 GATGCCCGGT GGCTGGAGCG CATGTGCCCA GTATTGTGTG GTACAAAGAT GAAAGGCTCC 
     3181 TGGAGAAAGA GTCGGGAATC GACCTGGCAG ACTCCAATCA GAGGCTGAGC ATCCAGCGCG 
     3241 TGCGCGAGGA GGACGCAGGT CGTTATCTGT GCAGCGTGTG CAATGCCAAG GGCTGCGTAA 
     3301 ACTCCTCTGC CAGCGTGGCA GTGGAAGGCT CTGAAGATAA AGGCAGCATG GAGATTGTGA 
     3361 TACTCATTGG CACTGGCGTC ATCGCAGTTT TCTTCTGGGT CCTCCTCCTG CTCATCTTCT 
     3421 GTAACATGAA AAGGCCTGCC CATGCAGACA TCAAGACGGG CTACCTGTCC ATCATCATGG 
     3481 ACCCCGGGGA GGTGCCTTTG GAGGAGCAGT GTGAATACCT GTCCTATGAC GCCAGCCAGT 
     3541 GGGAGTTCCC CAGGGAAAGG TTGCACCTCG GGAGAGTCCT AGGCCACGGG GCTTTTGGGA 
     3601 AGGTGGTGGA AGCCTCAGCT TTTGGCATCA ATAAAGGCAG CAGCTGTGAC ACCGTGGCTG 
     3661 TGAAGATGCT GAAAGAGGGC GCTACTGCCA GCGAGCACCG TGCCCTGATG TCGGAGCTCA 
     3721 AGATCCTAAT TCACATCGGC AACCATCTCA ACGTGGTCAA CCTCCTAGGG GCGTGCACCA 
     3781 AGCCCAACGG CCCTCTCATG GTGATCGTGG AGTTTTGCAA ATACGGCAAC CTCTCCAACT 
     3841 TCTTGCGTGT CAAGCGGGAC ACGTTCAACC CCTACGCGGA GAAGTCTCCG GAGCAACGCA 
     3901 GGCGCTTCCG CGCCATGGTA GAAGGCGCCA AAGCTGATAG GAGGAGACCT GGAAGCAGCG 
     3961 ACAGGGCCCT GTTCACGCGG TTCCTGATGG GCAAAGGAAG TGCACGGCGA GCCCCACTTG 
     4021 TCCAAGAAGC TGAGGACCTA TGGCTGAGCC CACTGACCAT GGAAGACCTT GTATGCTACA 
     4081 GCTTCCAAGT TGCCCGGGGA ATGGAGTTCC TGGCTTCCCG CAAGTGCATT CACAGAGACC 
     4141 TGGCTGCTCG GAACATCCTA CTGTCAGAAA GTGACATAGT GAAGATCTGC GACTTTGGCC 
     4201 TCGCTCGGGA CATCTACAAA GACCCCGACT ATGTCCGAAA GGGCAGTGCC CGACTTCCTC 
     4261 TGAAATGGAT GGCCCCCGAG AGCATCTTTG ATAAGGTGTA CACCACGCAG AGTGATGTGT 
     4321 GGTCCTTCGG CGTGCTGCTG TGGGAGATCT TCTCATTGGG GGCCTCTCCA TACCCTGGGG 
     4381 TACAGATCAA TGAGGAGTTC TGCCAGCGGC TGAAGGATGG CACTCGAATG AGAGCCCCGG 
     4441 AACTGGCCAC TCCTGCCATA CGCCACATCA TGCAGAGTTG CTGGTCTGGA GACCCTAAAG 
     4501 CAAGACCTGC TTTCTCTGAC CTAGTGGAGA TCCTGGGGGA CCTGCTTCAG GGCGGAGGCT 
     4561 GGCAGGAGGA GGAAGAGGAG CGCATGGCCC TGCACAGCTC TCAGAGCTCC GAGGAGGATG 
     4621 GCTTCATGCA GGCATCCACC ACAGCTCTAC ATATCACCGA AGCAGACGCT GATGATAGTC 
     4681 CACCCAGCAT GCATTGCCAC AGCCTGGCAG CCAGATATTA CAACTGTGTG TCCTTTCCTG 
     4741 GGCGCCTGGC CAGAGGCACT AAGACTCCAG GCTCTTCCAG GATGAAGACA TTTGAAGAAT 
     4801 TGCCCATGAC CCCTACAACC TACAAAGCCT CCATGGATAA CCAGACAGAC AGCGGGATGG 
     4861 TGCTGGCCTC AGAAGAGTTT GAGGAGCTAG AAAGCAGGCA TAGACCAGAA GGCAGCTTCA 
     4921 GCTGTAAAGG TCCTGGCCAG CACATGGATA TTCCCAGAGG ACACCCTGAC CCCCAGGGGA 
     4981 GGCGGCGACG GCCCACTCAA GGGGCACAAG GAGGCAAGGT GTTTTATAAC AACGAGTATG 
     5041 GGGAGGTCTC CCAGCCATGT ACAGAAGGTG ACTGCTGCCC GTCTGCTGGC TCCACCTTCT 
     5101 TCGCAGACAG CAGCTACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5161 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5221 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5281 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5341 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5401 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5461 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5521 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5581 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5641 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5701 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5761 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5821 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5881 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5941 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6001 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6061 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6121 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6181 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6241 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6301 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6361 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6421 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6481 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6541 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6601 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6661 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6721 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6781 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6841 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6901 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6961 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7021 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7081 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7141 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7201 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7261 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7321 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7381 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7441 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7501 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7561 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7621 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7681 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7741 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7801 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7861 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7921 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7981 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8041 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8101 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8161 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8221 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8281 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8341 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8401 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8461 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8521 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8581 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8641 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8701 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8761 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8821 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8881 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8941 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//