LOCUS       MR225284                7830 bp    DNA     circular  SYN 
DEFINITION  Pkn2 (Myc-DDK-tagged) - Mouse protein kinase N2 (Pkn2)
ACCESSION   NM_178654
VERSION     NM_178654.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3987..4078 
                     /label="Myc-DDK"  
     misc_feature    4108..4693 
                     /label="PolyA signal"  
     misc_feature    4165..4184 
                     /label="Restriction Enzyme"  
     misc_feature    4969..5641 
                     /label="ColE1"  
     misc_feature    6071..6865 
                     /label="Kan/Neo"  
     misc_feature    6900..7202 
                     /label="SV40 ori"  
     misc_feature    7288..7765 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3969..3975 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3969 
                     /label="Pkn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTCCAAC CCCGACCGGG GGGAGATCCT CCTCACCGAG CTGCAGGGAG 
     1081 ATTCCCGGAC TCTTCCATTT TCTGAGAATG TGAGTGCTGT TCAGAAATTA GACTTTTCAG 
     1141 ATACAATGGT GCAACAGAAA TTGGACGACA TCAAGGATCG AATAAAGAGA GAAATAAGGA 
     1201 AAGAACTGAA AATCAAAGAA GGAGCTGAAA ACCTGAGGAA AGTTACAACA GATAAGAAAA 
     1261 ATTTGGCTTA TGTAGACAAC ATTTTGAAAA AATCAAATAA AAAATTAGAA GAATTACATC 
     1321 ACAAGCTACA AGAATTAAAT GCACACATCG TTGTATCGGA TCCAGAGGAC TCCACAGATT 
     1381 GTCCGAGGAC TCCAGATACT CCAAATAGTG ACTCTCGTAG TTCTACTAGC AACAATAGAC 
     1441 TGATGGCCTT ACAGAAACAA CTGGATATAG AACTTAAAGT AAAGCAGGGT GCAGAGAACA 
     1501 TGATACAGAT GTACTCAAAT GGCTCTTCAA AGGATCGGAA ACTCCATGGT ACAGCGCAGC 
     1561 AACTGCTACA GGATAGCAAG ACAAAAATAG AAGTCATCCG GATGCAGATT CTTCAGGCCG 
     1621 TCCAGACCAA TGAGCTGGCT TTTGATAATG CAAAACCTGT GATAAGCCCT CTTGAGCTTC 
     1681 GGATGGAAGA ATTAAGACAT CATTTTAAGA TAGAATTTGC AGTAGCCGAA GGTGCAAAGA 
     1741 ATGTCATGAA GTTACTTGGC TCAGGAAAAG TAACAGACAG AAAAGCGCTT TCAGAAGCAC 
     1801 AAGCGAGATT TAATGAATCA AGTCAGAAGT TGGACCTTTT AAAGTATTCA CTAGAGCAAA 
     1861 GGTTAAATGA GCTCCCCAGG AATCACCCCA AAAGCAGTGT CGTCATTGAG GAGCTTTCAC 
     1921 TGGTTGCGTC ACCAACCCTG AGTCCACGGC AGAGCATGCT GTCCACGCAG AACCAGTATA 
     1981 GCACACTGTC CAAGCCAGCG GCGCTAACCG GTACTTTGGA AGTTCGTCTT ATGGGGTGCC 
     2041 AAGATATCCT AGAGAATGTA CCTGGACGGT CAAAAGCAAC GTCGGTAGCA CTACCTGGGT 
     2101 GGAGTCCAAG TGACAACAGA TCGTCTTTTA TGAGCAGAAC CAGCAAGAGT AAAAGTGGGA 
     2161 GTAGTCGCAA CCTCCTGAAA ACGGATGACT TGTCCAATGA TGTTTGTGCT GTTTTGAAGC 
     2221 TAGATAATAC TGTGGTTGGT CAAACTAGCT GGAAACCCAT TTCAAATCAG TCGTGGGACC 
     2281 AGAAGTTTAC ACTGGAACTA GACAGGTCAC GTGAACTGGA AATTTCAGTT TATTGGCGTG 
     2341 ATTGGCGATC TCTGTGTGCT GTAAAATTTC TGAGGTTAGA AGATTTTTTA GACAACCAGC 
     2401 GGCATGGCAT GTGTCTCTAT TTGGAACCAC AGGGTACTTT GTTTGCAGAG GTTACTTTTT 
     2461 TTAATCCAGT TATTGAAAGA CGACCAAAAC TCCAAAGACA AAAGAAAATT TTTTCAAAAC 
     2521 AACAAGGCAA AACATTTCTC AGAGCTCCTC AGATGAATAT TAATATTGCC ACTTGGGGAA 
     2581 GACTAGTGAG ACGAGCTATT CCCACAGTAA ATCATTCTGG CACATTCAGC CCTCAGACTC 
     2641 CTGTGCCTGC AACAGTGCCA GTCGTTGACG CACGCATTCC TGATCTAGCA CCTCCGGCCA 
     2701 GTGACTCTAC AGTAACCAAA TTGGACTTTG ATCTTGAACC TGAACCTCCT CCAGCCCCAC 
     2761 CTCGTGCTTC TTCCCTTGGA GAGACAGATG AATCTTCTGA ATTGAGAGTT TTGGATATAC 
     2821 CTGGACAGGG TTCAGAAACT GTTTTCAATA TTGAGAATGA CAGAAATAAC CTGCGTCCAA 
     2881 AATCCAAATC TGAATATGAG CTGAGCATTC CTGACTCAGG TCGAAGCTGC TGGGGTGTTG 
     2941 GAGAGCTTGA TGACAAGAGA GCTCAGCAGA GGTTTCAGTT CAGTCTACAG GACTTCAGGT 
     3001 GCTGTGCTGT CTTGGGGAGA GGACACTTTG GAAAGGTGCT GTTGGCGGAA TATAAACACA 
     3061 CAAATGAAAT GTTTGCAATA AAAGCCTTAA AGAAAGGAGA CATTGTGGCA CGAGATGAAG 
     3121 TAGACAGCCT GATGTGTGAA AAAAGAATTT TTGAGACTGT GAATAGTGTC AGGCACCCGT 
     3181 TTCTAGTGAA CCTTTTTGCA TGTTTCCAAA CAAAAGAACA TGTTTGCTTC GTGATGGAGT 
     3241 ATGCTGCAGG TGGTGACCTG ATGATGCACA TCCATACTGA TGTCTTCTCT GAACCCAGAG 
     3301 CTGTATTTTA TGCAGCATGT GTAGTTCTTG GGTTGCAATA CTTACATGAA CACAAAATTG 
     3361 TTTATAGAGA TTTGAAGTTG GATAACTTGT TGCTAGATAC AGAAGGCTTT GTGAAAATTG 
     3421 CTGACTTTGG TCTTTGCAAA GAAGGAATGG GGTATGGAGA TAGGACAAGC ACATTTTGTG 
     3481 GTACTCCTGA GTTTCTTGCC CCAGAAGTCC TAACAGAAAC ATCGTACACA AGGGCTGTAG 
     3541 ATTGGTGGGG CCTTGGTGTA CTTATATATG AGATGCTCGT CGGCGAGTCT CCCTTTCCTG 
     3601 GTGATGATGA AGAAGAGGTT TTTGACAGTA TTGTAAATGA CGAAGTAAGG TATCCAAGGT 
     3661 TCTTATCTAC AGAAGCCATT TCCATAATGA GAAGGCTGCT AAGGAGAAAT CCTGAGCGGC 
     3721 GCCTGGGAGC TGGTGAGAAG GATGCAGAGG ATGTCAAGAA GCACCCGTTT TTCCGGCTAA 
     3781 CCGACTGGAG TGCTCTAATG GACAAAAAAG TAAAGCCACC ATTTGTACCT ACCATTAGAG 
     3841 GACGGGAAGA TGTTAGTAAC TTTGATGATG AATTTACCTC AGAAGCACCT ATTCTGACTC 
     3901 CACCTCGAGA ACCAAGGATA CTCTTGGAAG AGGAGCAGGA GATGTTCCAC GATTTTGACT 
     3961 ACGTTGCTGA TTGGTGTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4021 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4081 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4141 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4201 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4261 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4321 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4381 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4441 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4501 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4561 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4621 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4681 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4741 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4801 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4861 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4921 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4981 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5041 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5101 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5161 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5221 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5281 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5341 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5401 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5461 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5521 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5581 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5641 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5701 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5761 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5821 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5881 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5941 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6001 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6061 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6121 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6181 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6241 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6301 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6361 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6421 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6481 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6541 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6601 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6661 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6721 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6781 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6841 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6901 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6961 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7021 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7081 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7141 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7201 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7261 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7321 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7381 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7441 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7501 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7561 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7621 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7681 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7741 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7801 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//