LOCUS       MR225278                8707 bp    DNA     circular  SYN 
DEFINITION  Adcy5 (Myc-DDK-tagged) - Mouse adenylate cyclase 5 (Adcy5)
ACCESSION   NM_001012765
VERSION     NM_001012765.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4864..4955 
                     /label="Myc-DDK"  
     misc_feature    4985..5570 
                     /label="PolyA signal"  
     misc_feature    5042..5061 
                     /label="Restriction Enzyme"  
     misc_feature    5846..6518 
                     /label="ColE1"  
     misc_feature    6948..7742 
                     /label="Kan/Neo"  
     misc_feature    7777..8079 
                     /label="SV40 ori"  
     misc_feature    8165..8642 
                     /label="f1 ori"  
     misc_feature    1060..1067 
                     /label="Restriction Enzyme"  
     misc_feature    4846..4852 
                     /label="Restriction Enzyme"  
     misc_feature    1060..4846 
                     /label="Adcy5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCAGAT CTCAAGCTTA ACTAGCTAGC GGACCGACAT GTCCGGCTCC 
     1081 AAAAGCGTGA GCCCCCCGGG CTACGCTGCA CAGACAGCGG CGTCGCCAGC GCCCCGGGGA 
     1141 GGCCCGGAGC ATCGCGCCGC TTGGGGAGAA GCCGATTCCC GCGCCAATGG CTACCCCCAC 
     1201 GCCCCCGGGG GATCAACCCG CGGCTCCACC AAGAGATCTG GGGGAGCGGT GACCCCACAA 
     1261 CAGCAGCAGC GCCTGGCCAG CCGTTGGCGC GGTGGCGATG ACGACGAAGA CCCTCCACTA 
     1321 AGCGGTGATG ACCCTCTGGC TGGGGGCTTC GGCTTCAGCT TCCGCTCTAA GTCCGCCTGG 
     1381 CAGGAGCGCG GTGGCGACGA CGGCGGTCGC GGCAGCAGGC GGCAGCGGCG GGGCGCGGCT 
     1441 GGAGGGGGCA GCACCCGGGC GCCCCCTGCG GGCGGCAGCG GCAGTTCGGC GGCGGCCGCA 
     1501 GCGGCCGCAG GTGGCACAGA GGTGCGCCCC CGCTCGGTGG AGCTGGGCCT GGAGGAGCGT 
     1561 CGAGGAAAAG GCCGAGCGGC CGAGGAGCTG GAGCCCGGGA CTGGCATCGT CGAGGATGGA 
     1621 GACGGGTCGG AGGATGGAGG CAGTTCTGTG GCGTCAGGCT CTGGGACCGG CGCGGTGCTG 
     1681 TCGTTGGGCG CCTGCTGCCT GGCCTTGCTG CAGATATTCC GCTCTAAGAA GTTCCCGTCG 
     1741 GACAAACTGG AGCGTCTGTA CCAGCGCTAC TTCTTCCGCC TGAACCAGAG CAGTCTCACC 
     1801 ATGCTCATGG CCGTGCTGGT GCTTGTGTGC CTGGTCATGC TGGCTTTCCA CGCGGCGCGC 
     1861 CCCCCGCTCC AGATAGCCTA CCTGGCCGTG TTGGCAGCTG CTGTGGGCGT GATCCTTATC 
     1921 ATGGCCGTGC TCTGCAACCG TGCAGCGTTC CACCAGGACC ACATGGGCCT GGCCTGCTAT 
     1981 GCGCTCATTG CAGTGGTGCT GGCCGTCCAG GTAGTGGGCC TGTTGCTGCC ACAGCCACGC 
     2041 AGCGCCTCCG AGGGCATCTG GTGGACCGTG TTCTTCATCT ATACCATCTA CACCCTGCTG 
     2101 CCTGTGCGCA TGAGGGCTGC GGTGCTCAGC GGGGTGCTTC TGTCGGCTCT CCACTTGGCC 
     2161 ATCTCTCTGC ACACCAACTC CCAGGACCAG TTTCTGCTGA AACAGCTTGT CTCCAATGTC 
     2221 CTCATCTTCT CCTGCACCAA CATTGTGGGT GTGTGCACTC ACTACCCAGC TGAGGTCTCC 
     2281 CAGAGACAGG CCTTCCAGGA GACCCGGGAG TGTATCCAGG CTCGGCTCCA TTCCCAGCGG 
     2341 GAGAACCAGC AACAGGAGCG TCTCCTGCTG TCTGTCCTTC CCCGTCATGT TGCCATGGAG 
     2401 ATGAAAGCAG ACATCAACGC CAAGCAGGAG GATATGATGT TCCACAAGAT CTACATCCAG 
     2461 AAGCATGACA ATGTGAGCAT CCTGTTTGCT GACATCGAGG GCTTCACCAG CCTGGCTTCC 
     2521 CAGTGTACTG CCCAAGAACT GGTCATGACC CTCAATGAGC TCTTCGCCCG CTTTGACAAG 
     2581 TTGGCTGCGG AGAATCACTG TTTACGGATT AAGATCCTCG GGGATTGTTA CTACTGCGTC 
     2641 TCGGGGCTGC CTGAAGCCAG AGCCGACCAT GCCCACTGCT GCGTGGAGAT GGGAATGGAC 
     2701 ATGATCGAGG CCATCTCGTT GGTCCGGGAG GTGACAGGGG TGAACGTGAA CATGCGCGTG 
     2761 GGAATTCACA GCGGGAGAGT ACACTGCGGT GTCCTTGGTC TCAGAAAGTG GCAATTCGAC 
     2821 GTGTGGTCTA ACGATGTCAC TCTGGCCAAC CACATGGAAG CTGGTGGCAA GGCGGGCCGC 
     2881 ATCCACATCA CCAAGGCCAC ACTCAACTAC CTGAATGGGG ACTATGAGGT GGAACCAGGC 
     2941 TGTGGTGGCG ATCGCAATGC CTACCTCAAG GAGCACAGCA TTGAGACCTT CCTCATCCTG 
     3001 AGCTGTACCC AGAAGCGGAA AGAAGAGAAG GCCATGATCG CCAAGATGAA CCGCCAGAGA 
     3061 ACCAACTCCA TCGGACACAA TCCGCCTCAC TGGGGAGCCG AGCGCCCCTT CTACAACCAC 
     3121 TTGGGCGGCA ACCAGGTGTC AAAGGAGATG AAGAGGATGG GCTTTGAGGA CCCCAAGGAC 
     3181 AAGAATGCCC AGGAAAGTGC GAACCCAGAG GATGAAGTGG ACGAGTTTCT GGGTCGGGCC 
     3241 ATCGATGCCA GGAGCATCGA CAGACTGCGA TCTGAACACG TCCGCAAGTT CCTCCTGACC 
     3301 TTCAGGGAGC CCGACTTAGA GAAGAAGTAC TCCAAGCAGG TGGATGACCG ATTTGGTGCC 
     3361 TATGTGGCCT GCGCCTCGCT TGTTTTCCTC TTCATCTGCT TTGTCCAGAT CACCATTGTG 
     3421 CCCCACTCCC TGTTCATGCT GAGTTTCTAC CTGTCGTGTT TCCTGCTGCT GGCCTTGGTG 
     3481 GTGTTTGTGT CTGTGATCTA TGCCTGTGTG AAGCTTTTCC CCACTCCCCT GCAGACACTC 
     3541 TCCAGGAAGA TAGTGCGATC CAAGAAGAAC AGCACCCTGG TCGGGGTATT CACCATCACC 
     3601 CTGGTGTTCC TCTCGGCTTT TGTCAACATG TTCATGTGCA ACTCTAAGAA CCTGGTGGGC 
     3661 TGCCTGGCAG AGGAGCACAA CATCACGGTG AACCAGGTCA ACGCATGTCA TGTGATGGAG 
     3721 TCGGCCTTCA ACTACAGCCT GGGCGACGAG CAGGGCTTCT GTGGCAGCCC CCAGCCCAAC 
     3781 TGCAACTTCC CAGAGTACTT CACCTACAGC GTGCTGCTCA GCCTGCTGGC CTGCTCCGTG 
     3841 TTCCTGCAGA TCAGCTGCAT TGGAAAGCTG GTGCTCATGC TGGCCATTGA GTTCATCTAT 
     3901 GTGCTCATCG TGGAGGTGCC CGGCGTCACA CTCTTCGACA ACGCTGACCT TCTGGTCACC 
     3961 GCCAATGCCA TAGACTTCAG CAACAACGGG ACCTCCCAGT GCCCTGAGCA TGCAACCAAG 
     4021 GTGGCGCTGA AGGTGGTGAC GCCCATCATC ATCTCTGTCT TCGTGCTGGC TCTGTATCTG 
     4081 CATGCCCAGC AGGTGGAGTC CACTGCTCGC CTTGACTTCC TCTGGAAACT GCAGGCCACA 
     4141 GAAGAGAAGG AGGAGATGGA GGAGCTGCAG GCCTACAATC GGCGCCTGCT GCACAACATT 
     4201 CTGCCCAAGG ACGTGGCTGC CCACTTCCTG GCCCGGGAAC GGCGCAATGA CGAGCTGTAC 
     4261 TACCAATCGT GCGAGTGTGT GGCTGTCATG TTTGCCTCCA TTGCCAACTT CTCCGAATTC 
     4321 TATGTAGAGC TAGAGGCCAA CAATGAGGGC GTCGAGTGCC TACGGCTGCT CAATGAGATC 
     4381 ATCGCAGACT TCGATGAGAT CATCAGTGAG GATCGGTTCA GGCAGCTGGA AAAGATCAAG 
     4441 ACCATAGGCA GCACCTACAT GGCTGCCTCT GGCCTCAACG ACTCCACCTA TGACAAGGCA 
     4501 GGCAAGACCC ACATCAAGGC TATTGCAGAC TTCGCTATGA AGCTGATGGA CCAAATGAAG 
     4561 TACATCAATG AGCACTCCTT CAACAACTTC CAGATGAAGA TTGGGCTCAA CATTGGACCT 
     4621 GTAGTGGCTG GAGTCATTGG GGCTCGCAAG CCTCAGTATG ACATCTGGGG CAATACAGTG 
     4681 AATGTGGCCA GCCGTATGGA CAGCACTGGG GTGCCTGACC GCATCCAGGT TACTACAGAT 
     4741 ATGTACCAGG TGCTGGCCGC CAACACATAC CAGCTGGAGT GCCGGGGTGT GGTCAAGGTC 
     4801 AAGGGCAAGG GTGAGATGAT GACTTACTTC CTCAATGGAG GGCCTCCCCT CAGTACGCGT 
     4861 ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG 
     4921 GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG 
     4981 AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT 
     5041 TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA 
     5101 CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT 
     5161 TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC 
     5221 ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC 
     5281 TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG 
     5341 GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT 
     5401 CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC 
     5461 TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT 
     5521 ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG 
     5581 CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT 
     5641 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     5701 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     5761 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     5821 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     5881 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     5941 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     6001 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     6061 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     6121 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     6181 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     6241 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG 
     6301 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     6361 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     6421 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     6481 AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT 
     6541 TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA 
     6601 CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG 
     6661 ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA 
     6721 TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC 
     6781 CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG 
     6841 CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT 
     6901 CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA 
     6961 GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC 
     7021 GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT 
     7081 AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC 
     7141 AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC 
     7201 GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG 
     7261 CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG 
     7321 TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT 
     7381 ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA 
     7441 TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT 
     7501 GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC 
     7561 TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG 
     7621 GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC 
     7681 CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC 
     7741 TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA 
     7801 GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG 
     7861 CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG 
     7921 GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT 
     7981 GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG 
     8041 ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC 
     8101 ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA 
     8161 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT 
     8221 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC 
     8281 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC 
     8341 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT 
     8401 CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC 
     8461 TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC 
     8521 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT 
     8581 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 
     8641 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 
     8701 GAATTTT
//