LOCUS       MR225263L1                9114 bp    DNA     circular  SYN 
DEFINITION  Trpc3 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 3 (Trpc3)
ACCESSION   NM_019510
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7403..7583 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8311..8970 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7742..8310 
                     /label="ColE1"  
     misc_feature    6278..6298 
                     /label="LR50 primer"  
     misc_feature    6075..6146 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6287..6878 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6050..6057 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6050 
                     /label="Trpc3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCCACCAAG GTCAAGAAGT GCCGGGAGCC 
     3361 CGCGAGGGTG ACCTTGCCCG CTCCGGAGGA GGAGGAGGAT GGCGAGGCCG AGGGAGGCGA 
     3421 GTCGCAGCGC CGCCGCCGGG GCTGGAGGGG CGTCAACGGC GGGCTGGAGC CGCCCTGCCC 
     3481 GCGCGCGCCG CCGTCCCCGG GGCCCGACGC GTCGTCGGAG GGCAGCCCAT CCAGGTGGCG 
     3541 CACTGCGGGG ATGCGTGACA AGGGCCGGCG CCAGGCAGTG CGTGGCCCGG CCTTCATGTT 
     3601 CGGTGCTCGT GGGCCCAGCC TCACAGCTGA GGAGGAGCGC TTCCTGGATG CTGCGGAGTA 
     3661 CGGCAACATC CCTGTGGTGC GCAAGATGCT GGAGGAGTCT CGCACGCTCA ATGTCAACTG 
     3721 CGTGGACTAC ATGGGCCAGA ACGCACTGCA GCTGGCTGTA GGCAATGAGC ATCTGGAGGT 
     3781 GACCGAGCTG CTGCTGAAGA AGGAGAACCT GGCGCGGATC GGTGATGCGC TGCTGCTAGC 
     3841 CATCAGCAAG GGCTATGTGC GCATCGTGGA GGCCATCCTG GGCCACCCAG GCTTTGCAGC 
     3901 CAGCCGGCGC CTGACCCTCA GCCCTTGCGA GCAAGAACTG CGAGATGATG ACTTCTATGC 
     3961 TTACGACGAG GATGGCACAC GCTTCTCGCC TGACATCACG CCCATCATCC TGGCTGCACA 
     4021 CTGCCATAAG TACGAGGTGG TGCACCTGCT GCTACTCAAG GGTGCACGCA TCGAGAGGCC 
     4081 ACACGACTAC TTCTGTCGCT GCTCTGACTG CGCTGAGAAA CAAAGGCTTG ACGCCTTCAG 
     4141 CCACTCAAGG TCTAGGATCA ATGCCTACAA GGGACTGGCC AGCCCAGCAT ACTTGTCGCT 
     4201 GTCCAGCGAG GACCCCGTGC TCACAGCACT GGAGCTCAGC AATGAGCTGG CCAAGCTGGC 
     4261 CAACATAGAG AAGGAGTTCA AGAATGACTA CAGGAAACTC TCCATGCAAT GCAAAGACTT 
     4321 CGTAGTGGGT GTGCTGGACC TGTGTCGGGA CTCGGAGGAG GTGGAAGCCA TTCTGAATGG 
     4381 AGATCTGGAA TCAGCCGAGC CCCTGGAAAG ACACGGGCAC AAAGCGTCAC TGAGTCGTGT 
     4441 CAAACTTGCC ATTAAGTATG AAGTCAAAAA GTTTGTGGCT CACCCCAACT GCCAACAGCA 
     4501 GCTTTTGACA ATCTGGTATG AGAACCTTTC CGGCCTCCGG GAGCAGACCA TCGCTATCAA 
     4561 GTGTCTGGTC GTGTTGGTCG TGGCCTTGGG TCTTCCATTC CTCGCCATCG GCTATTGGAT 
     4621 TGCACCTTGT AGCAGGCTGG GGAAGATTCT TCGAAGCCCC TTCATGAAGT TTGTGGCCCA 
     4681 CGCTGCCTCC TTCATCATCT TTCTGGGTCT GCTTGTATTC AACGCCTCGG ACAGATTTGA 
     4741 AGGCATCACC ACGCTGCCAA ACATCACTGT TATCGACTAC CCCAAGCAAA TCTTCAGGGT 
     4801 GAAGACCACC CAGTTCACGT GGACGGAAAT GTTAATTATG GTCTGGGTTC TTGGGATGAT 
     4861 GTGGTCTGAG TGCAAGGAGC TGTGGCTGGA GGGGCCCCGG GAGTACATCG TGCAGTTGTG 
     4921 GAATGTGCTT GATTTCGGCA TGCTCTCCAT CTTCATCGCC GCGTTCACAG CCAGGTTCCT 
     4981 CGCTTTCCTG CAGGCCACGA AGGCGCAGCA GTATGTGGAT AGTCACGTGC AGGAGAGCGA 
     5041 TCTGAGCGAA GTCACGCTCC CACCTGAGGT TCAATATTTC ACCTATGCTA GAGACAAATG 
     5101 GCTCCCCTCA GACCCTCAGA TCATATCTGA AGGGCTCTAT GCCATAGCTG TGGTGCTCAG 
     5161 CTTCTCCCGG ATTGCGTACA TCCTCCCTGC AAACGAGAGC TTTGGGCCCT TGCAGATCTC 
     5221 TCTTGGAAGG ACTGTGAAGG ACATATTCAA GTTCATGGTT CTCTTCATTA TGGTGTTCCT 
     5281 GGCTTTCATG ATTGGCATGT TCATACTTTA TTCTTACTAC CTTGGGGCCA AAGTAAATCC 
     5341 TGCTTTTACC ACGGTTGAAG AAAGTTTCAA GACTTTGTTT TGGTCCATAT TTGGACTGTC 
     5401 TGAAGTGACT TCTGTTGTCC TCAAATATGA TCACAAATTC ATAGAGAATA TTGGCTATGT 
     5461 TCTTTATGGG ATATATAATG TAACTATGGT GGTCGTTTTA CTCAACATGC TAATTGCTAT 
     5521 GATTAATAGC TCATACCAAG AGATCGAGGA TGACAGTGAT GTAGAATGGA AGTTTGCTCG 
     5581 TTCCAAACTT TGGCTCTCCT ACTTCGATGA TGGAAAAACA TTACCTCCAC CCTTCAGTCT 
     5641 GGTCCCTAGT CCAAAATCAT TTGTTTACTT CATCATGAGG ATCACTAACT TTTCCAAATG 
     5701 CAGGAGGAGA AGACTGCAGA AGGATCTGGA ACTGGGCATG GGTAACTCAA AGTCCAGGTT 
     5761 AAACCTCTTC ACACAGTCTA ACTCGAGAGT TTTTGAATCA CACAGTTTTA ACAGCATTCT 
     5821 CAATCAGCCA ACACGATATC AGCAGATAAT GAAAAGACTC ATAAAACGGT ATGTTTTGAA 
     5881 AGCACAAGTA GACAAAGAAA ACGATGAGGT GAATGAAGGT GAACTGAAAG AAATCAAGCA 
     5941 GGATATCTCC AGCCTTCGTT ATGAACTTTT AGAAGATAAG AGCCAAGCGA CGGAGGAATT 
     6001 AGCCATCTTG ATTCATAAAC TCAGTGAGAA ACTGAACCCC AGTGTGCTGA GATGTGAGAG 
     6061 CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC 
     6121 AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCT 
     6181 GTACAAGTAG GATTCGTCGA GGGACCTAAT AACTTCGTAT AGCATACATT ATACGAAGTT 
     6241 ATACATGTTT AAGGGTTCCG GTTCCACTAG GTACAATTCG ATATCAAGCT TATCGATAAT 
     6301 CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT 
     6361 TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG 
     6421 GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG 
     6481 CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT 
     6541 TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT 
     6601 GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG 
     6661 GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC 
     6721 TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT 
     6781 CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC 
     6841 CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGA 
     6901 CCTCGATCGA GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA 
     6961 TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC 
     7021 TCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA 
     7081 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT 
     7141 GTGGATCTAC CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG 
     7201 GATCAGATAT CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA 
     7261 GGTAGAAGAA GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG 
     7321 GATGGATGAC CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA 
     7381 TCACATGGCC CGAGAGCTGC ATCCGGACTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG 
     7441 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC 
     7501 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT 
     7561 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGCATG TGAGCAAAAG GCCAGCAAAA 
     7621 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     7681 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     7741 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     7801 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     7861 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     7921 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     7981 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     8041 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     8101 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     8161 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     8221 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     8281 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG ATTACGCCCC GCCCTGCCAC 
     8341 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG 
     8401 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG 
     8461 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG 
     8521 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG 
     8581 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC 
     8641 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA 
     8701 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA 
     8761 CGGAACTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC 
     8821 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG 
     8881 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG 
     8941 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TACTCTTCCT TTTTCAATAT 
     9001 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     9061 AAAAATAAAC AAATAGGGGT CCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAC
//