LOCUS       MR225230                8259 bp    DNA     circular  SYN 
DEFINITION  Dlc1 (Myc-DDK-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 3
ACCESSION   NM_001194941
VERSION     NM_001194941.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4416..4507 
                     /label="Myc-DDK"  
     misc_feature    4537..5122 
                     /label="PolyA signal"  
     misc_feature    4594..4613 
                     /label="Restriction Enzyme"  
     misc_feature    5398..6070 
                     /label="ColE1"  
     misc_feature    6500..7294 
                     /label="Kan/Neo"  
     misc_feature    7329..7631 
                     /label="SV40 ori"  
     misc_feature    7717..8194 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4398..4404 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4398 
                     /label="Dlc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGACCCC GAGGGCCACG TGATGGCTCG GCCCCTGAGA GGCCCGCTGC 
     1081 GGAGGTCCTT CAGCGACCAC ATCAGAGACT CCACCGCCAG GGCGCTGGAT GCTATCTGGA 
     1141 AAAACACCAG AGAGAGACGG CTAGCAGAAA TTGAAGCCAA GGAAGCTTGC GACTGGCTGA 
     1201 GAGTGACTGG TTTCCCCCAG TATGCACAGC TGTATGAAGA TCTACTGTTC CCTATTGATA 
     1261 TTGCGCTGGT CAAGAGAGAA CATGACTTCT TGGACAGAGA CGCCATCGAG GCTCTCTGCA 
     1321 GGCGCTTAAA TACTCTAAAC AAGTGTGCAG TCATGAAGCT GGAGATCAGT CCTCACCGGA 
     1381 AGCGAAGTGA GGATTCGGAT GAAGATGAGC CTTGCGCCAT AAGCGGCAAG TGGACTTTCC 
     1441 AGAGGGACAG CAAGCGGTGG TCCCGCCTTG AAGAGTTTGA CGTCTTTTCC CCAAAGCAGG 
     1501 ATCCAATCCC TGGGTCCCCA GACAACTCTC GTTTGCAAAG CGCCACAAGC CACGAAAGCA 
     1561 TGCTGACAGA CCTCAGCGAG CACCAGGAGG TGGCCTCTGT CCGAAGCCTC AGCAGCACCA 
     1621 GCAGCAGCGT CCCCACCCAC GCAGCCCACA GTGGAGATGC CACTACGCCC CGAACCAATT 
     1681 CCGTCATCAG CGTCTGCTCC TCCGGACACT TTGTAGGCAA TGATGACTCT TTTTCCAGCC 
     1741 TGCCGTCTCC CAAGGAACTG TCCAGCTTCA GTTTTAGCAT GAAAGGCCAC CACGAGAAGA 
     1801 ACACCAAGTC GAAGACGCGG AGCCTGCTCA AACGCATGGA GAGCCTGAAG CTCAAGGGCT 
     1861 CCCACCACAG CAAGCACAAG GCGCCTTCCA AGCTGGGGTT GATCATCAGT GCTCCCATTC 
     1921 TGCAGGAGGG TATGGATGAG GCAAAGCTGA AGCAGCTGAA CTGTGTGGAG ATCTCAGCCC 
     1981 TCAATGGCAA CCACATCAAC GTGCCCATGG TACGGAAAAG GAGCGTGTCT AACTCCACCC 
     2041 AGACCAGCAG CAGCAGCAGC CAATCAGAGA CCAGCAGCGC GGTCAGCACA CCCAGCCCGG 
     2101 TCACCAGGAC CCGGAGCCTC AGCACCTGTA ACAAGCGGGT GGGCATGTAT CTAGAGGGCT 
     2161 TCGACCCATT CAGTCAGTCC ACCTTGAACA ACGTGACGGA GCAGAACTAT AAAAACCGTG 
     2221 AGAGCTACCC AGAGGACACG GTGTTCTACA TTCCCGAAGA TCACAAGCCC GGCACCTTCC 
     2281 CTAAGGCCCT CTCCCATGGC AGTTTCTGTC CCTCGGGAAA CAGTTCTGTG AACTGGAGGA 
     2341 CCGGAAGCTT CCATGGCCCT GGCCATCTCA GCCTACGGAG AGAAAACAGC CATGACAGTC 
     2401 CTAAGGAGCT GAAGAGACGC AATTCTTCCA GCTCTCTGAG CAGCCGCCTG AGCATCTATG 
     2461 ATAACGTACC GGGTTCTATC CTGTACTCCA GCTCGGGAGA ACTGGCCGAC CTGGAGAATG 
     2521 AGGACATCTT CCCTGAGCTG GATGACATTC TCTACCACGT GAAGGGGATG CAGCGGATAG 
     2581 TCAACCAGTG GTCCGAGAAG TTTTCCGACG AGGGAGACTC GGACTCAGCC CTGGACTCTG 
     2641 TCTCTCCTTG CCCGTCATCT CCAAAACAGA TACACCTGGA TGTGGACCAT GACCGAAGGA 
     2701 CACCCAGTGA CCTGGACAGC ACAGGCAACT CCCTCAATGA GCCCGAAGAG CCCACTGATA 
     2761 TCCCGGAAAG AAGAGACTCA GGGGTGGGGG CTTCCCTGAC CAGGTGCAAT AGGCACAGAC 
     2821 TGAGGTGGCA CAGCTTCCAG AGCTCCCACC GGCCGAGCCT CAACTCCGTG TCACTGCAGA 
     2881 TTAACTGCCA GTCTGTGGCC CAGATGAACC TGCTGCAGAA ATACTCGCTC CTGAAATTGA 
     2941 CGGCCCTGCT GGAAAAGTAC ACGCCTTCCA ACAAGCACGG CTTCAGCTGG GCTGTGCCCA 
     3001 AGTTCATGAA AAGGATCAAG GTTCCAGACT ACAAGGACCG GAGTGTGTTT GGGGTCCCAC 
     3061 TGACTGTGAA CGTGCAGCGC TCAGGACAGC CCCTGCCCCA GAGCATCCAG CAGGCCATGC 
     3121 GCTACCTCCG TAACCACTGT CTGGACCAGG TCGGGCTCTT CAGGAAGTCA GGTGTCAAAT 
     3181 CCCGGATCCA GGCTCTACGC CAGATGAATG AAAGCGCTGA AGATAATGTC AACTATGAAG 
     3241 GCCAGTCTGC TTATGATGTG GCAGACATGT TAAAGCAATA TTTTCGAGAT CTTCCTGAGC 
     3301 CCCTCATGAC GAACAAACTC TCCGAAACCT TCCTGCAGAT CTACCAGTAT GTTCCCAAGG 
     3361 ACCAGCGCCT CCAAGCCATC AAGGCGGCCA TTATGCTCCT GCCCGACGAG AACCGGGAGG 
     3421 TTCTGCAGAC ACTTCTCTAT TTCCTGAGCG ATGTCACAGC GGCTGTGAAA GAAAACCAGA 
     3481 TGACTCCCAC CAACCTGGCT GTGTGCCTAG CTCCGTCCCT CTTCCACCTC AACACCCTGA 
     3541 AGCGAGAGAA TTCTTCTCCA AGGGTAATGC AAAGAAAACA GAGTTTGGGC AAACCAGACC 
     3601 AGAAAGACCT GAATGAAAAC CTAGCGGCGA CTCAAGGGCT GGCCCACATG ATTGCTGAGT 
     3661 GCAAGAAGCT CTTCCAGGTC CCTGAGGAAA TGAGCCGGTG CCGTAACTCC TACACTGAAC 
     3721 AAGAGCTGAA GCCCCTTACC CTGGAGGCCT TGGGACACCT GAATAGTGAC CAGCCTGCTG 
     3781 ACTACAGACA CTTCCTCCAG GACTGTGTGG ATGGCCTGTT TAAGGAGGTC AAAGAGAAGT 
     3841 TCAAAGGCTG GGTCAGCTAC CCCACCTCCG AACAGGCTGA GCTGTCCTAT AAGAAGGTCA 
     3901 GCGAAGGACC CCCGTTAAGG CTTTGGAGGT CAACTATCGA AGTCCCCGCT GCACCCGAGG 
     3961 AGATCTTAAA GCGCCTTCTG AAGGAGCAAC ACCTCTGGGA TGTGGACCTG CTGGACTCCA 
     4021 AGGTGATTGA AATCCTGGAC AGCCAGACTG AAATCTACCA ATACGTCCAA AACAGCATGG 
     4081 CGCCCCACCC TGCGCGGGAC TACGTCGTAT TGAGGACCTG GAGGACTAAC TTACCCAGGG 
     4141 GGGCCTGTGC TCTCTTACTC ACCTCTGTGG ATCACGACCG GGCCCCCGTG GCGGGGGTGA 
     4201 GGGTTAATGT GCTCCTGTCC AGATATTTGA TTGAACCCTG CGGGTCAGGA AAATCCAAGC 
     4261 TTACCTACAT GTGCAGAGCT GATTTAAGGG GCCACATGCC GGAGTGGTAC TCAAAATCTT 
     4321 TTGGACATTT GTGTGCAGCC GAAGTGGTAA AGATCCGAGA CTCCTTCAGT AATCAGAACA 
     4381 CTGAAAGCAA GGACACGAGA TCTAGGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4441 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4501 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4561 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4621 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4681 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4741 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4801 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4861 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4921 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4981 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5041 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5101 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5161 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5221 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5281 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5341 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5401 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5461 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5521 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5581 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5641 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5701 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5761 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5821 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5881 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5941 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6001 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6061 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6121 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6181 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6241 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6301 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6361 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6421 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6481 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6541 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6601 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6661 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6721 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6781 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6841 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6901 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6961 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7021 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7081 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7141 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7201 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7261 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7321 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7381 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7441 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7501 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7681 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7741 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7801 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7861 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7921 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7981 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8041 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8101 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8161 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8221 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//