LOCUS       MR225202                8127 bp    DNA     circular  SYN 
DEFINITION  Rest (Myc-DDK-tagged) - Mouse RE1-silencing transcription factor (Rest)
ACCESSION   NM_011263
VERSION     NM_011263.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4284..4375 
                     /label="Myc-DDK"  
     misc_feature    4405..4990 
                     /label="PolyA signal"  
     misc_feature    4462..4481 
                     /label="Restriction Enzyme"  
     misc_feature    5266..5938 
                     /label="ColE1"  
     misc_feature    6368..7162 
                     /label="Kan/Neo"  
     misc_feature    7197..7499 
                     /label="SV40 ori"  
     misc_feature    7585..8062 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4266..4272 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4266 
                     /label="Rest"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACCCAG GTGATGGGGC AGTCTTCTGG AGGAGGCAGT CTCTTCAACA 
     1081 ACAGTGCCAA CATGGGCATG GCCTTAACCA ACGACATGTA CGACCTGCAC GAGCTCTCGA 
     1141 AAGCTGAACT GGCAGCCCCT CAGCTCATCA TGTTAGCCAA CGTGGCCCTG ACGGGGGAGG 
     1201 CAAGCGGCAG CTGCTGCGAT TACCTGGTCG GTGAAGAGAG GCAGATGGCC GAATTGATGC 
     1261 CCGTGGGAGA CAACCACTTC TCAGAAAGTG AAGGAGAAGG CCTGGAAGAG TCGGCTGACC 
     1321 TCAAAGGGCT GGAAAACATG GAACTGGGAA GTTTGGAGCT AAGTGCTGTA GAACCCCAGC 
     1381 CCGTATTTGA AGCCTCAGCT GCCCCAGAAA TATACAGCGC CAATAAAGAT CCCGCTCCAG 
     1441 AAACACCCGT GGCGGAAGAC AAATGCAGGA GTTCTAAGGC CAAGCCCTTC CGGTGTAAGC 
     1501 CTTGCCAGTA CGAAGCCGAA TCTGAAGAGC AGTTTGTGCA TCACATCCGG ATTCACAGCG 
     1561 CTAAGAAGTT CTTTGTGGAG GAAAGTGCAG AGAAACAGGC CAAAGCCTGG GAGTCGGGGT 
     1621 CGTCTCCGGC CGAAGAGGGC GAGTTCTCCA AAGGCCCCAT CCGCTGTGAC CGCTGTGGCT 
     1681 ACAATACCAA CCGGTATGAC CACTACATGG CACACCTGAA GCACCACCTG CGAGCTGGCG 
     1741 AGAACGAGCG CATCTACAAG TGCATCATCT GCACGTACAC GACGGTCAGC GAGTACCACT 
     1801 GGAGGAAACA CCTGAGAAAC CATTTCCCCA GGAAAGTCTA CACCTGCAGC AAGTGCAACT 
     1861 ACTTCTCAGA CAGAAAAAAT AACTACGTTC AGCACGTGCG AACTCACACA GGAGAACGCC 
     1921 CGTATAAATG TGAACTTTGT CCTTACTCAA GCTCTCAGAA GACTCATCTA ACGCGACACA 
     1981 TGCGGACTCA TTCAGGTGAG AAGCCATTTA AATGTGATCA GTGCAATTAT GTGGCCTCTA 
     2041 ATCAGCATGA AGTGACCCGA CATGCAAGAC AGGTTCACAA CGGGCCTAAA CCTCTTAATT 
     2101 GCCCGCACTG TGACTACAAA ACAGCAGATA GAAGCAACTT CAAAAAGCAC GTGGAGCTGC 
     2161 ATGTTAACCC ACGGCAGTTC AACTGCCCCG TGTGTGACTA CGCGGCTTCT AAGAAGTGTA 
     2221 ATCTACAATA CCATTTCAAA TCTAAGCATC CCACCTGTCC CAGCAAAACA ATGGATGTCT 
     2281 CCAAAGTGAA GCTAAAGAAA ACCAAAAAGA GAGAGGCTGA CCTGCTTAAT AACGCCGTCA 
     2341 GCAACGAGAA GATGGAGAAT GAGCAAACAA AAACAAAGGG GGATGTGTCT GGGAAGAAGA 
     2401 ACGAGAAACC TGTAAAAGCT GTGGGAAAAG ATGCTTCAAA AGAGAAGAAG CCTGGTAGCA 
     2461 GTGTCTCAGT GGTCCAGGTA ACTACCAGGA CTCGGAAGTC AGCGGTGGCG GCGGAGACTA 
     2521 AAGCAGCAGA GGTGAAACAC ACAGACGGAC AAACAGGAAA CAATCCAGAA AAGCCCTGTA 
     2581 AAGCCAAGAA AAACAAAAGA AAGAAGGATG CTGAGGCCCA TCCCTCCGAA GAGCCTGTGA 
     2641 ACGAGGGACC AGTGACAAAA AAGAAAAAGA AGTCTGAGTG CAAATCAAAA ATCGGTACCA 
     2701 ACGTGCCAAA GGGCGGCGGC CGAGCGGAGG AGAGGCCGGG GGTCAAGAAG CAAAGCGCTT 
     2761 CCCTTAAGAA AGGCACAAAG AAGACGCCGC CCAAGACAAA GACAAGTAAA AAAGGTGGCA 
     2821 AACTTGCTCC AAAGGGGATG GGGCAGACAG AACCTTCTTC TGGGGCATTG GCTCAAGTGG 
     2881 GGGTGTCTCC AGACCCTGCC CTCATTCAGG CAGAGGTCAC CGGGTCAGGA TCTTCTCAGA 
     2941 CAGAGCTTCC TTCACCCATG GATATTGCTA AGTCAGAGCC CGCCCAGATG GAGGTTTCCC 
     3001 TAACAGGGCC ACCTCCGGTG GAGCCTGCTC AAATGGAGCC ATCGCCTGCG AAACCTCCCC 
     3061 AGGTAGAAGC ACCCACTTAC CCCCAGCCTC CCCAAAGGGG GCCTGCCCCT CCCACGGGGC 
     3121 CTGCCCCTCC CACGGGGCCT GCCCCTCCCA CGGAGCCTGC CCCTCCCACG GGGCTTGCCG 
     3181 AGATGGAACC TTCTCCCACG GAGCCTTCCC AGAAGGAACC ACCTCCCAGT ATGGAGCCTC 
     3241 CCTGCCCCGA GGAGCTGCCT CAGGCCGAGC CACCTCCTAT GGAGGATTGT CAGAAGGAGC 
     3301 TGCCTTCTCC CGTGGAGCCC GCTCAGATTG AGGTTGCTCA GACGGCCCCT ACGCAGGTTC 
     3361 AGGAGGAGCC CCCTCCTGTC TCGGAGCCAC CTCGGGTGAA GCCAACCAAA AGATCATCTC 
     3421 TCCGGAAAGA CAGAGCAGAG AAGGAGCTGA GCCTGCTGAG TGAGATGGCG CGGCAGGAGC 
     3481 AGGTCCTCAT GGGGGTTGGC TTGGTGCCTG TTAGAGACAG CAAGCTTCTG AAGGGAAACA 
     3541 AGAGCGCCCA GGACCCCCCA GCCCCACCGT CACCATCGCC AAAGGGAAAC TCGAGGGAAG 
     3601 AGACACCCAA GGACCAAGAA ATGGTCTCTG ATGGGGAAGG AACTATAGTA TTCCCTCTCA 
     3661 AGAAAGGAGG ACCAGAGGAA GCTGGAGAGA GTCCAGCTGA GTTGGCTGCT CTCAAGGAGT 
     3721 CTGCCCGTGT TTCATCCTCT GAACAAAACT CAGCCATGCC AGAGGGTGGA GCATCACACA 
     3781 GCAAGTGTCA GACTGGCTCC TCTGGGCTTT GTGACGTGGA CACTGAGCAG AAGACAGATA 
     3841 CTGTCCCCAT GAAAGACTCC GCAGCAGAGC CAGTGTCCCC TCCTACCCCA ACAGTGGACC 
     3901 GTGACGCAGG GTCACCAGCT GTAGTGGCCT CCCCTCCTAT CACGTTGGCT GAAAACGAGT 
     3961 CTCAGGAAAT TGATGAAGAT GAAGGCATCC ATAGCCATGA TGGAAGTGAC CTGAGTGACA 
     4021 ACATGTCTGA GGGGAGTGAC GACTCAGGAC TGCACGGGGC TCGGCCGACA CCACCAGAAG 
     4081 CTACGTCAAA AAATGGGAAG GCAGGGTTGG CTGGTAAAGT GACTGAGGGA GAGTTTGTGT 
     4141 GTATTTTCTG TGATCGTTCT TTTAGAAAGG AAAAAGATTA TAGCAAACAC CTCAATCGCC 
     4201 ACTTGGTGAA TGTGTACTTC CTAGAAGAAG CAGCTGAGGA GCAGGAGGAG CAGGAGGAGC 
     4261 GGGAGGAGCA GGAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4321 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4381 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4441 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4501 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4561 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4621 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4681 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4741 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4801 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4861 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4921 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4981 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5041 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5101 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5161 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5221 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5281 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5341 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5401 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5461 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5521 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5581 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5641 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5701 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5761 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5821 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5881 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5941 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6001 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6061 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6121 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6181 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6241 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6301 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6361 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6421 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6481 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6541 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6601 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6661 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6721 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6781 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6841 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6901 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6961 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7021 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7081 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7141 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7201 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7261 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7321 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7381 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7441 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7501 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7561 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7621 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7681 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7741 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7801 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7861 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7921 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7981 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8041 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8101 ATTTAACAAA AATTTAACGC GAATTTT
//