LOCUS       MR225181                7077 bp    DNA     circular  SYN 
DEFINITION  Gpr149 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 149 (Gpr149)
ACCESSION   NM_177346
VERSION     NM_177346.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3234..3325 
                     /label="Myc-DDK"  
     misc_feature    3355..3940 
                     /label="PolyA signal"  
     misc_feature    3412..3431 
                     /label="Restriction Enzyme"  
     misc_feature    4216..4888 
                     /label="ColE1"  
     misc_feature    5318..6112 
                     /label="Kan/Neo"  
     misc_feature    6147..6449 
                     /label="SV40 ori"  
     misc_feature    6535..7012 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3216..3222 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3216 
                     /label="Gpr149"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTTCTTT CTCAGCAACT TAACAAATGA CTCTAACCTG TGGAAAGCAA 
     1081 GTCATAATTC TACGGAGACG GACCTTATGA ATTCACCAGC AACTCTGACT CTTTCTCTCT 
     1141 TTTGCCTGAT CTGTATAATG ACTCTCGCAG CTCTGGTGGG CAGCATTTTC TCACTGGTTT 
     1201 CGCTGCTGAC CATGCAATAC AGAACTGTTT TGTCCATACT TGTGACATCT TGGTCTGTGG 
     1261 ATGATCTCTT GAGCGTCTTG TCCGTGGCCA TCTTCATGGT TTTACAGTGG CCAAAAGAGG 
     1321 CACAGGGCTA CTTCCAGTCT CTGTGCACCA CCTCTGCCCT ACTGTATATG TGCCAGGGCC 
     1381 TCTCCAGCAA CTTGAAGGCG ACTCTCATAG TCTGCTACAA CTTTTATACG ATGAACAGGA 
     1441 CGGTGGAGAG TCAGTCAAGC TCCTGGCGAT TAGGACAGGT ACTTGGTGTG ACTTTAACCG 
     1501 TTTGGGCAGT CAGCCTGCTG CTGGCTTCGC TCCCGCTCTG TGGCTGGGGT GTCTTCGTGC 
     1561 GCACGCCTTG GGGCTGCCTG ACCGACTGCT CCAGCCCATA CGTGCTGCTC CTCTTCGCGG 
     1621 TATACGCTTC CGCTTTCGGG CTCCTGGCGG TTCTCTCTGT CCCTCTCACT CACCAGCTGC 
     1681 TGTGTTCGGA GGAACCGCTG AGACTCCATG CCAACTACCA GGAAATTTCT CGTGGCGCCT 
     1741 CTACTCCTGG GACCCCTGCT GCTGCGGGAA GAGTGCTCTG TCTGCCGCCA GAGGATGTTG 
     1801 AGATCCCTGC TCTGCGATGC ACTGGGGGAT GCTCCCCGAG CTCCGACGTG GTGTTTGCCC 
     1861 CGGGTCAGCC GGCTGCCTCC GGCGCTGGAG CCGGCAGGCG AGAGAATCCC GGGACTCCCC 
     1921 AGGGCACCAA CAGCTTCCCC TTGAGCTTGG CACAGAAGCG CTTTTCTTTG ATTCTAGCGC 
     1981 TGACGAAAGT CATCCTTTGG CTACCCATGA TGATCCACAT GGTGGTAAAA CACGTGGTGG 
     2041 GGTTTCAGAG CCTTCCGGTC GATATGCTCA GCTTTCTACT AAGCCTGCTG GCCAGCAGTG 
     2101 TAACACCAGT GTTTGTCTTA TCCAAACGTT GGGCCCACTT GCCTTGTGGC TGCATCATCA 
     2161 ACTGCCAGCC AGACACCTAC TCCGTTGCCT TCGATGGGAA AAAGTCGAAG AGGAAAGGCT 
     2221 TTGAATTCAA TCTATCATTC CAACAAAGTT ATGGACTCTA TAAAATACCA CATGCAGATT 
     2281 GCTATGATGA TGATGAAAAT TCCATATCCT ATCACAACCC AAAGAATTAT GAGTGTGAAG 
     2341 CTACAAAGGA GCCTTGGGGA GACAACCGCA GTGTCTTCAA TACCATCACA GTAGAAATCA 
     2401 GCACCACGCC ACCGCTGGAC AGCGCCACAC TGACGGGTGT CCACAAGTGT ACCAATACTG 
     2461 ACATCCCAGA ATCCAAACAG GCCATGAAAG AGGAAAAGGG TGCTTTTTCT GTTAAAACAG 
     2521 AAAGCGATAT TAACTATGGA GAAACCACCT CTTTTGAAGG TCCAGAGAGA CGACTGTCCC 
     2581 ATGAGGAGAA TCAGAAACCA GATCTTTCAG ACTGGGAATG GTGCAGGAGT AAGTCTGAAC 
     2641 GAACGCCTCG CCAGCGTTCC GGTGGTGGGC TGGCTATTCC CATATGCGCG TTCCAGGGGA 
     2701 CTGTGTCTCT CCAAGCACCT ACTGGGAAAA CGCTCTCTCT TTCTACTTAT GAGGTCAGCG 
     2761 CAGAAGGGCA AAAGATAACC CCAGCCTCTA AGAAAATAGA AGTCTATCGA TCTAAAAGTG 
     2821 TTGGCCATGA ACCGAACTCA GAGGAGTCTC CCTCCACGTT TGCGGACACC AGTGTAAAAA 
     2881 TACACTTGGA AGTTCTTGAA ATTTGTGACA ACGATGAGGC TCTAGACACT GTGTCAATTA 
     2941 TCAGCAACAT CAGCCAATCC TCCACAAAAG TCAGGTCTCC TTCTCTGCGC TACTCACGGA 
     3001 AGGAAAACAG ATTCGTCTCC TGCGACTTAG GAGAAACTGC CTCATACTCG CTCTTTTTAC 
     3061 CCACAAGTGA TCCTGATGGT GATATTAACA TCTCTATCCC TGACACGGTG GAGGCCCACA 
     3121 GACAGAACAG CAGGCGGCAG CATGAAGAGA GGGATGGCTA CCAGGAAGAG ATCCAGTTGT 
     3181 TAAACAAAGC CTACAGGAAA AGAGAGGCAG AAAGCAAGGG TGATACGCGT ACGCGGCCGC 
     3241 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3301 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3361 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3421 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3481 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3541 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3601 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3661 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3721 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3781 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3841 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3901 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3961 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4021 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4081 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4141 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4201 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4261 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4321 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4381 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4441 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4501 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4561 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4621 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4681 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4741 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4801 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4861 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4921 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4981 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5041 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5101 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5161 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5221 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5281 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5341 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5401 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5461 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5521 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5581 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5641 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5701 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5761 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5821 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5881 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5941 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6001 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6061 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6121 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6181 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6241 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6301 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6361 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6421 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6481 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6541 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6601 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6661 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6721 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6781 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6841 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6901 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6961 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7021 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//