LOCUS       MR225176                8313 bp    DNA     circular  SYN 
DEFINITION  Adcy3 (Myc-DDK-tagged) - Mouse adenylate cyclase 3 (Adcy3), transcript variant 3
ACCESSION   NM_001159537
VERSION     NM_001159537.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4470..4561 
                     /label="Myc-DDK"  
     misc_feature    4591..5176 
                     /label="PolyA signal"  
     misc_feature    4648..4667 
                     /label="Restriction Enzyme"  
     misc_feature    5452..6124 
                     /label="ColE1"  
     misc_feature    6554..7348 
                     /label="Kan/Neo"  
     misc_feature    7383..7685 
                     /label="SV40 ori"  
     misc_feature    7771..8248 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4452..4458 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4452 
                     /label="Adcy3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGAGGAAC CAGGGCTTCT CGGATCCCGA GTACTCGGCA GAGTACTCAG 
     1081 CTGAATACTC GGTCAGCCTG CCCTCTGACC CCGACCGCGG GGTCGGCCGG ACCCATGAAA 
     1141 TTTCTGTGCG GAACTCGGGG TCCTGCCTGT GCCTGCCTCG CTTTATGCGG CTGACCTTCG 
     1201 TGCCTGAGTC CTTGGAGAAC CTCTACCAGA CCTACTTCAA AAGGCAGCGC CATGAGACCC 
     1261 TGCTGGTGCT GGTGGTCTTT GCGGCCCTCT TTGACTGCTA CGTGGTAGTG ATGTGCGCGG 
     1321 TGGTCTTCTC CAGCGACAAG CTGGCGCCCC TCATGGTGGC AGGCTTCGGT CTGGTGTTGG 
     1381 ACATCATCCT TTTCGTGCTC TGTAAAAAGG GGCTGCTCCC GGACCGAGTG AGCCGCAAAG 
     1441 TGGTACCCTA CCTGCTGTGG CTGCTCATCT CGGCCCAGAT CTTCTCCTAC CTGGGCCTGA 
     1501 ACTTTTCACG GGCCCACGCA GCCAGTGACA CTGTGGGTTG GCAGGCTTTC TTTGTCTTCT 
     1561 CCTTCTTCAT AACGCTGCCC CTCAGCCTCA GCCCCATCGT GATCATCTCT GTGGTCTCCT 
     1621 GTGTTGTGCA CACGCTTGTG TTGGGGGTCA CTGTGGCCCA GCAGCAGCAG GATGAGCTGG 
     1681 AAGGGATGCA GCTGCTGAGG GAGATCCTGG CCAACGTCTT CCTCTACCTG TGTGCTATCA 
     1741 TCGTGGGCAT CATGTCCTAC TACATGGCAG ACCGCAAGCA CCGCAAGGCC TTCCTGGAGG 
     1801 CCCGCCAGTC ACTGGAGGTG AAGATGAATC TGGAGGAGCA GAGCCAGCAG CAGGAAAACC 
     1861 TTATGCTTTC CATCCTGCCC AAGCACGTGG CTGACGAGAT GCTGAAGGAC ATGAAGAAGG 
     1921 ACGAGAGCCA GAAGGACCAG CAGCAGTTCA ATACCATGTA CATGTACCGG CACGAGAATG 
     1981 TCAGCATCCT GTTTGCAGAT ATTGTGGGCT TTACCCAGCT GTCCTCTGCT TGCAGTGCCC 
     2041 AGGAGCTCGT GAAGTTACTC AACGAGCTCT TCGCCCGCTT TGACAAGCTG GCAGCCAAAT 
     2101 ACCACCAGCT GAGGATCAAG ATCCTAGGCG ACTGTTACTA CTGCATCTGC GGCTTGCCTG 
     2161 ACTACCGGGA GGACCATGCC GTGTGCTCCA TCCTTATGGG GCTTGCCATG GTAGAGGCCA 
     2221 TCTCGTACGT GCGGGAGAAG ACCAAGACTG GAGTGGACAT GCGTGTGGGA GTGCACACAG 
     2281 GCACTGTCCT AGGCGGTGTC CTGGGCCAGA AGCGCTGGCA GTATGATGTA TGGTCTACTG 
     2341 ATGTCACCGT GGCAAACAAG ATGGAGGCTG GTGGCATCCC AGGGCGCGTG CACATTTCCC 
     2401 AGAGCACCAT GGACTGCCTG AAGGGGGAGT TTGATGTGGA GTCTGGTGAT GGGGGCAGTC 
     2461 GCTGCGACTA CCTAGATGAG AAGGGCATCG AAACCTACCT CATCATTGCC TCCAAGCCAG 
     2521 AGGTGAAGAA AACAGCCCAA AATGGCCTCA ACGGCTCGGC CGTGCCAAAC GGAGCGCCGG 
     2581 CATCCTCCAA ACCCAGCTCC CCTGCCCTCA TCGAGACCAA GGAGCCCAAT GGAAGTGCCC 
     2641 ATGCCAGCGG CTCCACATCA GAGGAGGCTG AAGAACAGGA GGCCCAGGCT GACAACCCCT 
     2701 CGTTCCCCAA CCCCCGCCGC AGGCTGCGCC TCCAGGACCT GGCGGACCGT GTGGTGGACG 
     2761 CCTCTGAGGA TGAGCACGAA CTGAACCAGC TGCTTAATGA GGCCCTGCTG GAGCGGGAGT 
     2821 CCGCCCAGGT GGTAAAGAAG AGAAACACAT TCCTCCTGAC CATGAGGTTC ATGGACCCAG 
     2881 AGATGGAAAC ACGCTACTCG GTGGAGAAGG AGAAGCAGAG CGGGGCTGCC TTCAGCTGCT 
     2941 CCTGTGTGGT CCTCTTCTGC ACGGCCATGG TGGAGATACT CATTGACCCC TGGCTGATGA 
     3001 CAAACTATGT GACCTTTGTG GTTGGGGAGG TTCTGCTCCT GATCCTGACC ATCTGCTCGA 
     3061 TGGCTGCCAT CTTTCCCAGG TCATTTCCCA AGAAGCTTGT GGCCTTCTCA TCTTGGATTG 
     3121 ACCGGACCCG CTGGGCAAGG AACACCTGGG CCATGTTAGC CATCTTCATT CTGGTTATGG 
     3181 CCAATGTTGT GGACATGCTC AGCTGTCTCC AGTACTACAT GGGACCTTAC AACATGACAG 
     3241 CTGGGATGGA GCTGGACGGC GGCTGCATGG AGAACCCCAA GTACTACAAC TACGTAGCTG 
     3301 TGCTGTCGCT CATCGCCACC ATCATGCTGG TGCAGGTCAG CCACATGGTG AAGCTGACGC 
     3361 TCATGCTGCT CGTCACAGGC GCCGTGACTG CCCTCAACCT GTACGCCTGG TGTCCTGTCT 
     3421 TTGATGAATA CGACCACAAG CGCTTTCAGG AAAAGGACTC TCCTATGGTG GCCTTAGAGA 
     3481 AGATGCAGGT ACTTGCCACC CCTGGGCTCA ATGGCACTGA CAGGCTGCCC CTGGTGCCTT 
     3541 CCAAGTACTC CATGACTGTG ATGATGTTCG TCATGATGCT GAGCTTTTAC TACTTCTCGC 
     3601 GCCACGTGGA AAAACTGGCC CGAACACTGT TCTTGTGGAA GATTGAGGTC CATGACCAGA 
     3661 AAGAACGTGT CTACGAGATG CGCCGATGGA ACGAGGCCTT GGTCACCAAC ATGTTGCCTG 
     3721 AGCATGTTGC ACGCCATTTT CTGGGGTCCA AGAAGAGAGA TGAGGAGCTG TACAGCCAGT 
     3781 CTTATGACGA GATTGGAGTC ATGTTTGCCT CCTTGCCCAA CTTTGCTGAC TTCTACACTG 
     3841 AGGAGAGCAT CAACAACGGC GGCATCGAGT GTCTACGCTT CCTCAATGAG ATCATCTCTG 
     3901 ATTTTGACTC TCTCCTGGAC AATCCCAAAT TCCGGGTCAT CACCAAGATC AAAACTATTG 
     3961 GCAGCACCTA TATGGCAGCT TCAGGAGTCA CACCAGATGT CAACACCAAT GGCTTTACAA 
     4021 GCTCCAGCAA GGAGGAGAAG TCAGACAAGG AGCGCTGGCA GCACCTGGCT GACCTGGCTG 
     4081 ACTTTGCATT AGCCATGAAG GACACGCTCA CAAACATCAA CAACCAGTCA TTCAACAACT 
     4141 TCATGTTGCG CATAGGCATG AACAAAGGAG GGGTTCTGGC TGGCGTCATT GGAGCCCGGA 
     4201 AACCACACTA TGACATCTGG GGCAATACGG TCAATGTGGC CAGCAGGATG GAATCCACAG 
     4261 GAGTCATGGG CAACATCCAG GTGGTAGAAG AGACGCAGGT CATCCTTCGA GAGTACGGCT 
     4321 TCCGCTTTGT GAGGCGAGGT CCCATCTTTG TGAAAGGCAA AGGGGAGCTT CTGACCTTTT 
     4381 TCTTGAAGGG GCGGGACAGG CCAGCTGCCT TCCCCAATGG CTCCTCTGTT ACACTGCCCC 
     4441 ACCAAGTGGT GGACAACCCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4501 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5281 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5341 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5401 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5461 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5521 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5581 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5641 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5701 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5761 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5821 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5881 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5941 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6001 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6061 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6121 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6181 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6241 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6301 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6361 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6421 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6481 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6541 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6601 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6661 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6721 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6781 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6841 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6901 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6961 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7021 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7081 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7141 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7201 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7261 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7321 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7381 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7441 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7501 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7561 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7741 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7801 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7861 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7921 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7981 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8041 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8101 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8161 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8221 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8281 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//