LOCUS       MR225166                8955 bp    DNA     circular  SYN 
DEFINITION  Tnr (Myc-DDK-tagged) - Mouse tenascin R (Tnr)
ACCESSION   NM_022312
VERSION     NM_022312.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5112..5203 
                     /label="Myc-DDK"  
     misc_feature    5233..5818 
                     /label="PolyA signal"  
     misc_feature    5290..5309 
                     /label="Restriction Enzyme"  
     misc_feature    6094..6766 
                     /label="ColE1"  
     misc_feature    7196..7990 
                     /label="Kan/Neo"  
     misc_feature    8025..8327 
                     /label="SV40 ori"  
     misc_feature    8413..8890 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5094..5100 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5094 
                     /label="Tnr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGATCGAT GGGGAAACAG TAGTCTTGAA GAACATGCTC ATTGGTGTAA 
     1081 ACCTGATCCT GTTGGGATCC ATGCTCAAGC CTTCTGAGTG TCGGCTGGAG GTGACTACAG 
     1141 AAAGGGCTCA GAGACAAACT GTGGAGGAGG AAGGAGGGGC TTCCAGCTAC AATACTTCCA 
     1201 GCAAGGAACA GCCTATGGTC TTCAACCATG TGTATAACAT CAACGTGCCA CTTGAAAGCC 
     1261 TCTGCTCCTC GGGGCTGGAG GCCTCAGCTG AGCAGGACAT GAGTGCTGAA GATGATACTC 
     1321 TGGCCGAATA CATAGGCCAA ACCTCAGACC ATGAAAGCCA GGTTACCTTC ACCCACAAGA 
     1381 TCAACCTCCC CAAAAAAGCC TGCCCATGTG CAAGCTCTTC CCAGGTACTG CAGGAACTGC 
     1441 TGAGCCGGAT CGAGATGCTG GAGAGGGAGG TGTCCTTGCT GCGAGACCAG TGCAATACCA 
     1501 ACTGCTGTCA GGAAAGCGCT GCCACAGGAC AACTGGACTA TGTCCCTCAC TGCAGCGGCC 
     1561 ATGGCAACTT TAGCTTCGAG TCCTGTGGCT GCATCTGCAA TGAAGGCTGG TTTGGCAAGA 
     1621 ACTGCTCAGA GCCCTACTGC CCACTGGGCT GCTCCAGTCG GGGTGTGTGT GTCGATGGCC 
     1681 AGTGCATTTG TGACAGTGAA TACAGCGGAG ATGACTGTTC AGAGCTCCGG TGCCCAACAG 
     1741 ACTGCAGTTC CCGAGGGCTC TGTGTGGATG GGGAATGTGT CTGTGAAGAG CCCTACACAG 
     1801 GCGAGGACTG CAGGGAGCTG CGCTGCCCTG GGGACTGTTC AGGGAAGGGG CAATGTGCCA 
     1861 ATGGTACCTG CCTGTGCCAA GAGGGCTATG CTGGTGAGGA CTGCAGCCAG CGACGGTGTC 
     1921 TGAATGCCTG CAGTGGGCGA GGTCACTGCC AGGAGGGGCT CTGCATCTGT GAGGAAGGCT 
     1981 ACCAGGGCCC TGACTGCTCA GCAGTTGCCC CTCCAGAGGA CTTGCGAGTG GCTGGTATCA 
     2041 GCGACAGGTC CATTGAGCTG GAATGGGACG GGCCGATGGC AGTGACGGAA TATGTGATCT 
     2101 CTTACCAGCC GACGGCCCTG GGGGGCCTTC AGCTCCAGCA GCGGGTGCCT GGAGATTGGA 
     2161 GTGGTGTCAC CATCATGGAG CTGGAGCCAG GTCTCACCTA CAACATCAGC GTCTACGCTG 
     2221 TCATTAGCAA CATCCTCAGC CTTCCCATCA CTGCCAAGGT GGCCACTCAT CTCTCCACTC 
     2281 CTCAAGGGCT ACAGTTCAAG ACGATCACAG AGACCACCGT GGAGGTGCAG TGGGAGCCCT 
     2341 TCTCTTTCTC CTTCGACGGG TGGGAGATCA GCTTCATTCC AAAGAACAAT GAAGGAGGGG 
     2401 TGATTGCTCA GCTCCCCAGC GATGTGACGT CCTTTAACCA GACAGGACTG AAACCTGGGG 
     2461 AGGAGTACAT TGTGAATGTT GTGGCTCTAA AGGAACAAGC CCGGAGCCCT CCTACCTCTG 
     2521 CCAGCGTCTC CACTGTCATT GACGGGCCCA CACAGATCCT GGTTCGAGAT GTCTCTGATA 
     2581 CTGTGGCTTT TGTGGAATGG ACCCCACCTC GAGCCAAAGT TGATTTCATT CTTTTAAAAT 
     2641 ATGGCTTGGT GGGTGGCGAA GGCGGGAAGA CGACCTTCCG GCTGCAGCCT CCCTTAAGCC 
     2701 AGTACTCCGT GCAAGCCCTT AGACCTGGCT CGCGCTACGA GGTGTCCATC AGCGCAGTCC 
     2761 GGGGGACCAA TGAGAGTGAA GCCTCAAGCA CCCAATTTAC AACAGAGATT GATGCTCCCA 
     2821 AGAATTTGCG AGTGGGTTCC CGCACAGCAA CTAGCCTTGA CCTGGAATGG GATAACAGCG 
     2881 AGGCCGAAGC TCAGGAGTAC AAGGTTGTGT ACAGCACCTT AGCTGGTGAA CAGTACCACG 
     2941 AAGTGCTGGT ACCCAAGGGC ATTGGCCCAA CTACCAAGAC TACCCTCACA GATCTGGTTC 
     3001 CAGGCACAGA GTATGGAGTT GGAATATCTG CAGTAATGAA CTCAAAACAA AGCATTCCTG 
     3061 CCACTATGAA TGCCAGGACT GAACTTGACA GTCCCCGAGA CCTCATGGTA ACAGCCTCCT 
     3121 CAGAGACCTC TATCTCTCTC ATCTGGACCA AGGCCAGTGG TCCCATTGAT CACTACAGAA 
     3181 TTACTTTTAC CCCATCTTCT GGGATCTCCT CCGAAGTCAC TGTGCCCAGG GATAGGACTT 
     3241 CATACACACT AACAGATCTA GAGCCTGGAG CAGAATACAT TATCTCCATC ACTGCTGAGA 
     3301 GGGGTCGGCA GCAGAGCCTG GAGTCTACTG TGGATGCCTT CACAGGCTTC CGCCCTATCT 
     3361 CCCATTTGCA CTTTTCTCAT GTGACCTCCT CCAGTGTCAA TATCACCTGG AGTGACCCAT 
     3421 CTCCCCCAGC AGACAGACTC ATTCTGAACT ACAGCCCCAG GGACAAAGAG GAAGACATGT 
     3481 TGGAGGTCCT CTTGGATGCC ACCAAGAGGC ACGCTGTCTT GATGGGTCTA CAGCCAGCCA 
     3541 CTGAATATAT AGTGAACCTT GTAGCTGTCC ATGGGACGGT AACCTCTGAA CCCATAGTGG 
     3601 GTTCTATCAC TACAGGAATT GATCCTCCCA AAAACATCAC AATTAGCAAC GTGACTAAGG 
     3661 ACTCCCTGAC GGTGTCCTGG AGCTCTCCTG TTGCGCCTTT TGATTACTAC CGAGTATCGT 
     3721 ACCGACCCAC CCAAGTGGGA CGGCTAGACA GCTCCGTAGT GCCCAACACC GTGACAGAGT 
     3781 TCGCCATCAC CAGGCTGTAT CCAGCTACTG AATATGAAAT AAGCCTCAAC AGTGTACGGG 
     3841 GCAGGGAGGA GAGTGAACGC ATCTGCACCC TGGTGCACAC AGCCATGGAT AGCCCCATGG 
     3901 ATTTGATCGC TACCAACATC ACACCTACAG AAGCCTTGCT CCAGTGGAAA GCACCCATGG 
     3961 GTGAAGTGGA AAATTATGTC ATCGTCCTCA CACACTTTGC AATTGCTGGA GAGACCATCC 
     4021 TGGTTGACGG GGTCAGCGAA GAATTCCAGC TTGTAGACTT GCTTCCTAGC ACCCACTACA 
     4081 CTGTCACTAT GTATGCCACC AGTGGGCCTC TCATGAGTGG CACCATTGCC ACCAACTTCT 
     4141 CCACCCTCCT GGACCCTCCT GACAACCTGA CAGCCAGTGA AGTCACCAGG CAAAGCGCAC 
     4201 TGATCTCCTG GCAGCCGCCC AGAGCTGCGA TTGAAAACTA TGTCTTGACA TACAAGTCCA 
     4261 CCGATGGAAG CCGCAAAGAG CTGATAGTGG ATGCTGAGGA CACCTGGATC CGACTAGAGG 
     4321 GCCTGTCAGA GAACACAGAC TACACAGTGC TCCTGCAGGC AGCCCAGGAG GCCACAAGGA 
     4381 GCAGTCTCAC CTCTACTGTC TTTACCACAG GGGGCCGGGT GTTCTCTCAT CCTCAAGACT 
     4441 GTGCCCAGCA TTTGATGAAT GGAGACACTC TGAGTGGCGT TTACACCATC TTCCTCAATG 
     4501 GGGAGCTAAG CCACAAGCTG CAAGTGTACT GTGACATGAC CACAGATGGG GGCGGCTGGA 
     4561 TTGTATTCCA GAGGCGGCAA AATGGACAAA CTGATTTTTT CCGGAAATGG GCAGATTATC 
     4621 GTGTTGGCTT TGGGAATCTG GAGGATGAGT TTTGGCTAGG GCTAGACAAC ATCCACAGGA 
     4681 TAACAGCCCA GGGCCGCTAT GAGCTGCGTG TGGATATGCG GGATGGACAG GAAGCAGTCT 
     4741 TTGCCTACTA TGACAAGTTC GCTGTGGAGG ACAGCAGAAG CCTGTACAAG ATCCGCATAG 
     4801 GAAGTTACAA TGGCACTGCA GGAGACTCCC TCAGCTATCA CCAGGGACGT CCTTTCTCCA 
     4861 CTGAGGACAG AGACAATGAT GTTGCAGTCA CCAACTGTGC CATGTCCTAC AAGGGTGCTT 
     4921 GGTGGTATAA GAACTGCCAC CGGACCAACC TCAACGGGAA GTACGGGGAG TCCAGGCACA 
     4981 GTCAGGGGAT CAACTGGTAC CATTGGAAAG GCCATGAATT CTCCATCCCC TTTGTAGAAA 
     5041 TGAAGATGAG GCCCTACATC CATCGTCTCA CAGCCGGGAG GAAACGGCGA GCCTTGAAAT 
     5101 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5161 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5221 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5281 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5341 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5401 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5461 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5521 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5581 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5641 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5701 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5761 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5821 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5881 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5941 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6001 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6061 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6121 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6181 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6241 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6301 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6361 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6421 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6481 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6541 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6601 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6661 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6721 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6781 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6841 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6901 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6961 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7021 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7081 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7141 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7201 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7261 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7321 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7381 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7441 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7501 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7561 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7621 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7681 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7741 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7801 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7861 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7921 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7981 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8041 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8101 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8161 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8221 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8281 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8341 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8401 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8461 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8521 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8581 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8641 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8701 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8761 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8821 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8881 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8941 TTTAACGCGA ATTTT
//