LOCUS       MR224972                8097 bp    DNA     circular  SYN 
DEFINITION  Gtf2ird1 (Myc-DDK-tagged) - Mouse general transcription factor II I repeat domain-containing 1 (Gtf2ird1), transcript variant 1
ACCESSION   NM_020331
VERSION     NM_020331.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4254..4345 
                     /label="Myc-DDK"  
     misc_feature    4375..4960 
                     /label="PolyA signal"  
     misc_feature    4432..4451 
                     /label="Restriction Enzyme"  
     misc_feature    5236..5908 
                     /label="ColE1"  
     misc_feature    6338..7132 
                     /label="Kan/Neo"  
     misc_feature    7167..7469 
                     /label="SV40 ori"  
     misc_feature    7555..8032 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4236..4242 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4236 
                     /label="Gtf2ird1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTTGCTG GGGAAGCACT GTGACATCCC CACCAACGGC TGTGGGTCTG 
     1081 AGCGCTGGAA CTCCACCTTC GCCCGCAAGG ACGAACTCAT CAACAGTCTG GTGTCCGCCT 
     1141 TAGACTCCAT GTGCTCGGCG CTCTCCAAGC TGAACACGGA GGTGGCCTGC GTGGCGGTAC 
     1201 ACAATGAGAG CGTCTTCGTG ATGGGCACCG AGAAGGGAAG GGTGTTTCTG AACACTCGGA 
     1261 AGGAGCTACA GTCAGACTTC CTCAGGTTCT GCCGGGGACC CCTGTGGAAC GATCCAGAAG 
     1321 CAGGACACCC TAAAAAGGTG CAGCGCTGTG AAGGCGGTGG CCGGAGCCTC CCGCGGTCCT 
     1381 CTCTGGAGCA GTGCTCGGAT GTGTACCTGC TGCAGAAGAT GGTAGAGGAA GTGTTTGATG 
     1441 TTCTTTATAG TGAGGCTATG GGCAGGGCCA CCGTGGTACC TTTGCCCTAT GAGAGGCTGC 
     1501 TCAGGGAGCC GGGGCTACTG GCGGTGCAGG GGCTGCCCGA GGGCCTGGCC TTCCGGAGGC 
     1561 CAGCAGAGTA TGACCCCAAG GCACTCATGG CCATATTGGA GCACAGTCAC CGAATTCGGT 
     1621 TTAAGCTCAG GAGGCCTCCT GATGACGGTG GGCAGGACAC GAAGGCGCTG GTGGAGATGA 
     1681 ACGGTATCTC TCTGCTACCC AAGGGGTCCC GAGACTGTGG TCTGCATGGC CAGGCCTCCA 
     1741 AGGTCGCTCC CCAAGACCTG ACCCCCACCG CCACCCCATC CTCTATGGCC AACTTCCTGT 
     1801 ACAGCACTTC GATGCCCAAC CACACGATCC GGGAACTCAA GCAGGAGGTG CCAACCTGCC 
     1861 CGTTGACCCC CAGCGACCTG GGCATGGGCT GGCCCGTGCC TGAGCCCCAT GTCCCCAGCA 
     1921 CCCAAGATTT CTCTGATTGC TGTGGACAGA CGCCTGCAGG GCCTGCTGGG CCTCTCATCC 
     1981 AGAATGTCCA TGCTTCCAAG CGCATCCTCT TCTCCATCGT CCATGACAAG TCAGAGAAGT 
     2041 GGGATCCCTT CATCAAGGAA ATGGAGGACA TCAATACCCT GCGGGAGTGC GTGCAGATTC 
     2101 TGTTTAACAG CAGATACGCG GAAGCCCTGG GCCTGGACCA CATGGTCCCT GTGCCCTATA 
     2161 GGAAGATTGC CTGTGACCCC GAGGCTGTGG AAATTGTGGG TATTCCAGAC AAGATCCCCT 
     2221 TCAAGCGACC CTGTACTTAC GGAGTGCCGA AGCTGAAGAG GATTCTGGAG GAGCGACACA 
     2281 GCATTCACTT CATTATCAAG AGAATGTTCG ATGAGCGCAT TTTCACAGGG AACAAGTTTA 
     2341 CCAAAGACCC CATGAAGCTG GAGCCAGCTA GCCCACCAGA AGACACTTCC ACAGAAGTCT 
     2401 GTAGGGACAG CATGCTGGAC CTGGCTGGGA CTGCTTGGTC AGACATGAGC AGCGTCTCTG 
     2461 AAGACTGTGG GCCAGGAACC TCAGGAGAGA TAGCAATGTT GAGGCCTATC AAAATCGAGC 
     2521 CAGAGGAGCT GGACATTATT CAGGTTACGG TCTCAGATCC TTCACCTACC TCTGAGGAGA 
     2581 TGACTGACTC GTTACCTGGG CATCTGCCCT CAGAGGATTC CGGTTATGGG ATGGAAATGC 
     2641 CGGCTGACAA AGGCCCCAGT GAAGAACCGT GGTCAGAAGA GAGGCCGGCC GAAGAGAGCC 
     2701 CTGGTGACGT GATCCGGCCC CTACGGAAGC AGGTGGAGAT GCTGTTCAAC ACGAAATATG 
     2761 CCAAAGCTAT TGGTACCTCA GAGCCGGTCA AGGTGCCCTA CTCCAAGTTC CTGATGCACC 
     2821 CGGAGGAGCT GTTCGTACTG GGACTGCCTG AAGGCATCTC TCTTCGGAGA CCCAACTGCT 
     2881 TTGGGATTGC AAAGCTGCGG AAGATTCTGG AAGCGAGCAA CAGCATCCAG TTTGTCATCA 
     2941 AGAGACCCGA ACTGCTCACT GACGGTGTCA AAGAACCTGT TCTGGACACT CAAGAGAGGG 
     3001 ACTCCTGGGA CCGTCTTGTG GACGAGACCC CGAAGAGACA GGGCCTTCAA GAAAATTACA 
     3061 ACACCAGACT CTCGCGGATC GACATCGCCA ACACGCTTAG GGAACAAGTC CAAGACCTGT 
     3121 TTAACAAGAA ATACGGTGAA GCTCTGGGCA TCAAATACCC AGTGCAGGTG CCCTACAAGA 
     3181 GAATCAAAAG CAACCCAGGC TCGGTAATCA TTGAAGGCCT ACCCCCCGGG ATCCCATTCC 
     3241 GCAAACCCTG CACCTTTGGC TCCCAGAACC TGGAAAGGAT TCTCTCTGTG GCTGACAAGA 
     3301 TCAAGTTCAC GGTCACCAGG CCATTCCAAG GACTTATCCC AAAGCCTGAA ACCAAAATTC 
     3361 TCACTACAGG ACATGAAGCT GGGAAAACCA CCAGACCAAG GAGACTGCAA CAGGACACCT 
     3421 GGCAGCCAGA TGAGGATGAT GCCAACAGAC TGGGGGAGAA GGTGATCCTC CGAGAGCAGG 
     3481 TGAAGGAGCT CTTCAATGAG AAATACGGTG AGGCCCTGGG ACTGAATCGG CCTGTGCTGG 
     3541 TCCCTTACAA ACTGATCCGG GACAGCCCAG ATGCCGTGGA GGTGAAGGGC CTCCCAGATG 
     3601 ACATCCCCTT CCGGAACCCC AACACCTATG ACATCCATCG GCTGGAGAAG ATCCTGAAGG 
     3661 CCAGGGAGCA TGTGCGGATG GTCATCATCA ACCAGCTCCA ACCCTTTGCG GAAGTCTGCA 
     3721 ATGACCCCAA GGTGCCAGAG GAGGATGACT CTAACAAGCT CGGGAAGAAG GTGATCCTCC 
     3781 GAGAGCAGGT GAAGGAGCTC TTCAATGAGA AATACGGTGA GGCCCTGGGA CTGAATCGGC 
     3841 CTGTGCTGGT CCCTTACAAA CTGATCCGGG ACAGCCCAGA TGCCGTGGAG GTGAAGGGCC 
     3901 TCCCAGATGA CATCCCCTTC CGGAACCCCA ACACCTATGA CATCCATCGG CTGGAGAAGA 
     3961 TCCTGAAGGC CAGGGAGCAT GTGCGGATGG TCATCATCAA CCAGCTCCAA CCTTTTGGGG 
     4021 ACGTCTGCAA CAATGCCAAG GTGCCAGCCA AAGACAACAT TCCCAAGCGC AAGAGAAAGA 
     4081 GGGTCTCTGA AGGCAACTCA GTCTCCTCTT CTTCCTCCTC TTCATCTTCG TCCTCTAACC 
     4141 CAGAGTCTGT GGCATCCACC AACCAGATCT CCCTCGTGCA GTGGCCAGTG TACATGGTGG 
     4201 ACTATTCCGG ACTAAACGTG CAGCTTCCGG GCCCCCTTGA TTATACGCGT ACGCGGCCGC 
     4261 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4321 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4381 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4441 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4501 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4561 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4621 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4681 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4741 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4801 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4861 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4921 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4981 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5041 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5101 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5161 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5221 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5281 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5341 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5401 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5461 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5521 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5581 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5641 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5701 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5761 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5821 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5881 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5941 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6001 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6061 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6121 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6181 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6241 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6301 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6361 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6421 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6481 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6541 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6601 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6661 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6721 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6781 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6841 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6901 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6961 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7021 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7081 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7141 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7201 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7261 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7321 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7381 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7441 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7501 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7561 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7621 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7681 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7741 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7801 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7861 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7921 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7981 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8041 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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