LOCUS       MR224971                8964 bp    DNA     circular  SYN 
DEFINITION  Pik3r4 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 4, p150 (Pik3r4)
ACCESSION   NM_001081309
VERSION     NM_001081309.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5119..5210 
                     /label="Myc-DDK"  
     misc_feature    5240..5825 
                     /label="PolyA signal"  
     misc_feature    5297..5316 
                     /label="Restriction Enzyme"  
     misc_feature    6101..6773 
                     /label="ColE1"  
     misc_feature    7203..7997 
                     /label="Kan/Neo"  
     misc_feature    8032..8334 
                     /label="SV40 ori"  
     misc_feature    8420..8897 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5094..5101 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5094 
                     /label="Pik3r4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAAATCAG CTGGCTGGCA TCGCTCCTTC CCAGATCCTG TCTGTAGAGA 
     1081 GTTATTTCTC AGACATCCAT GACTTTGAGT ATGATAAGAG CCTGGGAAGC ACTCGGTTTT 
     1141 TTAAAGTCGC TCGAGCCAAG CACCGGGAAG GCCTGGTGGT TGTGAAGGTC TTTGCAATTC 
     1201 AGGATCCCAC GTTACCCTTA ACTAGTTATA AACAGGAGCT GGAGGAACTG AAAATCAGGC 
     1261 TCCACTCCGC CCAGAACTGC CTCCCCTTCC AGAAAGCAGC AGAAAAGGCG TCTGAGAAAG 
     1321 CAGCCATGCT GTTCCGGCAG TATGTGAGAG ACAACCTCTA CGATCGCATC AGTACCCGCC 
     1381 CGTTCCTAAA CAACATCGAG AAGCGCTGGA TCGCCTTCCA GATCCTGACG GCCGTGGACC 
     1441 AAGCGCACAA ATCTGGAGTC CGCCACGGAG ACATCAAGAC AGAGAATGTG ATGGTCACCA 
     1501 GTTGGAACTG GGTCCTCCTA ACCGACTTCG CCAGTTTTAA GCCCACGTAT CTTCCCGAGG 
     1561 ACAACCCAGC GGATTTCAAC TATTTCTTTG ACACCTCGAG AAGGAGGACA TGCTACATAG 
     1621 CTCCCGAGCG CTTCGTTGAC GGTGGGATGT TCGCCACCGA GTTAGAGTAC ATGAGAGACC 
     1681 CCTCAACCCC GCTTGTCGAC CTGAATAGCA ATCAGAGAGC GCGGGGAGAG CTGAAGAGAG 
     1741 CCATGGACAT CTTTTCGGCA GGTTGCGTGA TAGCTGAGCT CTTTACGGAA GGCGTACCTT 
     1801 TGTTTGATCT CTCTCAGCTG CTGGCGTATA GAAACGGACA TTTTTTCCCT GAACAAGTGC 
     1861 TAAACAAAAT TGAAGATCGC AGTATCAGAG ACTTGGTAAC TCAGATGATT AACCGTGAGC 
     1921 CCGAGAAGCG TTTGGAAGCT GAGGACTACC TGAAGCAGCA GCGAGGCAAC GCCTTCCCTG 
     1981 AGATATTTTA TACTTTCCTT CAGCCTTACA TGGCCCAGTT CGCCAAGGAA ACCTTTCTCT 
     2041 CTGCAGATGA GCGGATTTTG GTTATACGGA AAGATTTGGG CAACATTATT CACAACCTCT 
     2101 GTGGACATGA TTTGCCAGAA AAAGCAGAAG GGGAGTCCAG GGCCAGTGGT CTGGTTGTCC 
     2161 TGGTGTCCGT GATAACGTCC TGCCTGCAGA CACTGAAGTC CTGCGACTCC AAACTGGCCG 
     2221 CCTTGGAGCT CATCCTGCAT CTGGCCCCCA GACTGAGCGT CGAGATCCTT CTGGATCGCA 
     2281 TCACTCCCTA CCTGTTGCAT TTCAGCAATG ACTCTGTCCC AAGAGTGCGG GCTGAAGCCC 
     2341 TGAGGACACT CACCAAAGTC CTTGCCCTTG TCCAAGAGGT TCCTCGCAAC GATGTCAATA 
     2401 TCTATCCAGA GTATATTCTC CCCGGCATAG CCCACCTGGC CCAGGATGAC GCTACCATCG 
     2461 TCAGACTGGC CTACGCTGAA AACATAGCTC TGTTGGCCGA GACGGCTCTG CGATTCCTGG 
     2521 AGCTTGTGCA GCTGAAAACT CTCAACATGG AGAACGAGCC CGATAACGAG GAGGTAGATG 
     2581 AAGCCACGCG TCCTAACGGA GACTATGACA CAGAGCTCCA GGCCTTGCAT GAGATGGTCC 
     2641 AGCAGAAGGT GGTCACGTTG CTAAGCGACC CTGAGAATAT CGTGAAACAG ACGCTGATGG 
     2701 AGAGTGGGAT CACACGCTTG TGCGTCTTCT TCGGACGCCA GAAGGCCAAC GACGTTCTCT 
     2761 TGTCCCACAT GATCACTTTC CTCAATGATA AGAATGATTG GCACCTGCGT GGAGCTTTCT 
     2821 TTGATAGCAT AGTCGGTGTG GCTGCCTACG TTGGCTGGCA GAGCTCCTCT ATCCTCAAGC 
     2881 CCCTGCTCCA GCAAGGCCTC AGCGATGCTG AGGAGTTTGT CATCGTGAAA GCTCTCAATG 
     2941 CCCTGACCTG CATGTGCCAG CTAGGGCTGC TGCAGAAACC CCATGTCTAC GAGTTCGCCA 
     3001 GTGATATTGC TCCCTTCCTG TGTCACCCTA ACCTGTGGAT ACGCTATGGC GCTGTGGGAT 
     3061 TTATCACGGT GGTAGCTCAT CAGATCAGTA CTGCTGATGT CTACTGCAAG CTCATGCCGT 
     3121 ATCTTGACCC GTATATTACA CAGCCAGTGA TACAGATTGA GAGGAAGCTG GTCCTGCTCA 
     3181 GTGTGCTAAA GGAGCCGGTG AGCCGCTCCA TATTTGACTA TGCTCTGAGG TCGAAAGACA 
     3241 TCGCCAGCTT GTTCAGACAT CTCCACATGC GCCAGAAGAA GCGAAATGGC TCTCTTCTCG 
     3301 ACTGCCCTCC ACCTGAGGAC CCCACCATAG CGCAACTTCT GAAGAAGTTG CTCTCACAGG 
     3361 GGATGACTGA AGAAGAGGAA GATAAACTTC TGGCACTGAA GGACTTCATG ATGAAATCAA 
     3421 ATCGAGCCAA AGCTAATGCG GTAGACCAGA GCCACCTCCA CGACAGCAGC CAGAAAGGTG 
     3481 TAATCGACTT GGCCGCCTTA GGCATCACTG GGAGACAAGT TGATCTTGTT AAAACCAAAC 
     3541 AAGAACCAGA TGAGAAACGT GCTAGGAAAC ATGTGAAACA AGACTCCAAT GTAAATGAAG 
     3601 AGTGGAAAAG CATGTTTGGG TCATTGGAGC CACCGAATAT CCCACAGGCC CTGCCTAAAA 
     3661 CCAGTGACCA TGAGGTTGTT CAGCCTGGGA AGCCACCTCG CTCTGAGTCC TCTGCTGGTA 
     3721 TCTGTGTCCC TTTGTCAACG TCTCCACAGG TCTCAGAAGC AGCTCACATC CCAAGTAAGA 
     3781 AGCCTGTGAT CCCGGTTGTG AGTAGCACGG TCCTGCCGTC CACTTACCAG ATCCGCATCA 
     3841 CCACGTGCAA GACTGAACTT CAGCAGCTCA TACAGCAGAA GAGGGAGCAG TGCAACGCGG 
     3901 AGCGCATAGC CAAGCAGATG ATGGAGAATG CCGAGTGGGA GAGCAAGCCA CCTCCACCTG 
     3961 GGTGGCGTCC TAAAGGGCTG CTAGTTGCAC ATCTTCATGA GCACAAATCT GCTGTGAATC 
     4021 GCATCAGAGT CTCTGATGAA CACTTACTTT TTGCAACATG TTCAAATGAT GGCACAGTGA 
     4081 AGATCTGGAA CAGCCAGAAG ATGGAGGGGA AGACCACCAC AACGCGGTCT ATTCTCACGT 
     4141 ACAGCCGAAT TGGAGGACGA GTCAAGACGC TAACGTTTTG CCAAGGCTCC CACTACTTGG 
     4201 CCATAGCATC TGATAATGGT GCTGTCCAGC TTCTTGGAAT TGAGGCGTCT AAGTTACCCA 
     4261 AGTCTCCTAA AATTCACCCT CTACAAAGCA GGATTCTGGA TCAGAAGGAA GATGGCTGTG 
     4321 TGGTGGACAT GCATCACTTC AACTCCGGGG CACAGTCCGT TCTTGCCTAT GCCACGGTGA 
     4381 ATGGCTCTCT GGTTGGATGG GATCTCAGGT CTTCAAGCAA CGCGTGGACA TTAAAGCATG 
     4441 ACCTAAAGTC AGGCCTCATC ACTTCCTTTG CCGTGGACAT CCACCAGTGC TGGCTGTGCA 
     4501 TAGGCACGAG CAGCGGTGCC ATGGCGTGTT GGGACATGAG GTTCCAGTTG CCCATCTCCA 
     4561 GTCACTGTCA TCCCTCCAGA GCTCGCATCC GGCGCCTCTC CATGCACCCC CTGTACCAGT 
     4621 CCTGGGTAAT CGCAGCTGTT CAGGGCAACA ATGAAGTCTC CATGTGGGAC ATGGAAACTG 
     4681 GCGACAGGAG ACTGACTCTC TGGGCCAGCA GCGCACCGCC ACTGTCTGAA TTACAGCCTT 
     4741 CACCGCACAG CGTCCATGGC ATCTACTGCA GCCCTGCAGA TGGAAACCCT ATCCTGCTGA 
     4801 CCGCTGGCTC AGACATGAAA ATAAGGTTCT GGGACTTGGT TTCCCCAGAG AGGTCCTATG 
     4861 TTGTTGCGGG AAGTACAGGT TCCCCGTCTG TCTCCTACTA CAAGAAGATA ATAGAAGGCA 
     4921 CCGAGGTCGT CCAGGAAATT CAGAATAAGC AGAAGGTTGG ACCGAGTGAT GATACCCCTC 
     4981 GGAGGGGCCC GGAGTCTCTG CCTGTGGGAC ATCATGACAT CATCACAGAC ATTGCCACCT 
     5041 TCCAGACCAC TCAGGGCTTC ATTGTGACTG CTTCTAGAGA CGGAATTGTG AAGGTGTGGA 
     5101 AAAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5161 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5221 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5281 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5341 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5401 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5461 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5521 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5581 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5641 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5701 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5761 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5821 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5881 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5941 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6001 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6061 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6121 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6181 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6241 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6301 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6361 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6421 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6481 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6541 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6601 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6661 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6721 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6781 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6841 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6901 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6961 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7021 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7081 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7141 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7201 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7261 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7321 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7381 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7441 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7501 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7561 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7621 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7681 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7741 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7801 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7861 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7921 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7981 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8041 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8101 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8161 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8281 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8341 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8401 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8461 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8521 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8581 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8641 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8701 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8761 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8821 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8881 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8941 TAACAAAAAT TTAACGCGAA TTTT
//