LOCUS       MR224909                9081 bp    DNA     circular  SYN 
DEFINITION  Brd4 (Myc-DDK-tagged) - Mouse bromodomain containing 4 (Brd4), transcript variant 1
ACCESSION   NM_020508
VERSION     NM_020508.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5238..5329 
                     /label="Myc-DDK"  
     misc_feature    5359..5944 
                     /label="PolyA signal"  
     misc_feature    5416..5435 
                     /label="Restriction Enzyme"  
     misc_feature    6220..6892 
                     /label="ColE1"  
     misc_feature    7322..8116 
                     /label="Kan/Neo"  
     misc_feature    8151..8453 
                     /label="SV40 ori"  
     misc_feature    8539..9016 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5220..5226 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5220 
                     /label="Brd4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTACGGAG AGCGGCCCTG GGACAAGATT GAGAAATCTG CCAGTAATGG 
     1081 GGGATGGACT AGAAACCTCC CAAATGTCTA CAACGCAGGC CCAGGCCCAA CCCCAGCCAG 
     1141 CAAATGCAGC CAGCACCAAT CCTCCACCCC CAGAGACCTC CAACCCTAAC AAGCCCAAGA 
     1201 GACAGACAAA CCAACTGCAA TATCTGCTCA GAGTGGTGCT CAAGACACTA TGGAAACACC 
     1261 AGTTTGCGTG GCCTTTCCAG CAGCCCGTGG ATGCCGTCAA GCTGAACCTC CCTGATTACT 
     1321 ATAAGATTAT TAAAACACCC ATGGATATGG GAACAATAAA GAAGCGCTTG GAAAACAACT 
     1381 ATTACTGGAA TGCTCAGGAA TGTATCCAGG ACTTCAACAC TATGTTTACA AATTGTTACA 
     1441 TCTATAACAA GCCTGGAGAT GACATCGTCT TAATGGCAGA AGCTCTGGAG AAGCTCTTCT 
     1501 TGCAAAAAAT CAATGAACTG CCTACAGAAG AAACTGAGAT CATGATAGTC CAGGCAAAAG 
     1561 GAAGAGGACG AGGGAGGAAA GAAACAGGGG CAGCAAAGCC TGGTGTATCC ACGGTACCAA 
     1621 ACACAACTCA AGCATCAACT TCTCCGCAGA CCCAGACGCC TCAGCAGAAC CCTCCTCCAC 
     1681 CTGTGCAGGC CACAACTCAC CCCTTTCCTG CTGTCACCCC AGACCTCATT GCCCAGCCTC 
     1741 CTGTCATGAC AATGGTGCCC CCTCAGCCAC TTCAGACTCC TTCACCGGTA CCCCCCCAGC 
     1801 CACCACCCCC ACCTGCTCCA GTTCCACAGC CTGTGCAGAG TCACCCGCCC ATCATTGCGA 
     1861 CCACCCCCCA GCCTGTGAAG ACAAAGAAAG GGGTGAAGAG GAAAGCAGAT ACCACCACCC 
     1921 CTACCACCAT CGACCCCATT CATGAGCCAC CCTCACTGGC CCCAGAGCCC AAGACCGCCA 
     1981 AGCTGGGTCC TCGGCGGGAG AGCAGCAGAC CTGTGAAGCC TCCAAAGAAG GATGTACCGG 
     2041 ACTCACAGCA GCACCCAGGG CCAGAGAAGA GCAGCAAGAT CTCTGAGCAG CTAAAGTGCT 
     2101 GCAGTGGCAT CCTCAAGGAG ATGTTTGCCA AGAAACATGC TGCCTATGCC TGGCCTTTCT 
     2161 ACAAGCCTGT GGATGTGGAG GCACTGGGTC TGCACGACTA CTGTGACATC ATCAAACATC 
     2221 CCATGGACAT GAGCACAATC AAGTCTAAAC TAGAGTCCCG AGAGTACAGA GATGCCCAGG 
     2281 AATTTGGTGC TGATGTCCGA TTGATGTTCT CCAACTGCTA CAAGTACAAC CCCCCTGACC 
     2341 ATGAAGTGGT AGCCATGGCT CGAAAACTCC AGGATGTGTT TGAAATGCGC TTTGCCAAGA 
     2401 TGCCTGATGA GCCTGAAGAG CCAGTTGTTA CAGTGTCCTC TCCTGCAGTG CCACCCCCTA 
     2461 CAAAGGTGGT AGCCCCACCC TCATCTAGTG ACAGCAGCAG CGACAGTTCT TCCGACAGCG 
     2521 ACAGTTCCAC TGACGACTCT GAGGAAGAGC GAGCCCAGCG GCTGGCTGAA CTCCAGGAAC 
     2581 AGCTCAAGGC CGTGCATGAG CAGCTTGCAG CCCTCTCACA GCCCCAGCAG AACAAACCAA 
     2641 AGAAAAAGGA GAAGGACAAG AAGGAAAAGA AAAAGGAAAA GCACAAAAAG AAAGAAGAAG 
     2701 TGGAGGAAAA TAAAAAAAGC AAAACCAAGG AACTTCCTCC CAAAAAGACA AAGAAAAATA 
     2761 ACAGCAGCAA CAGCAATGTG AGCAAGAAGG AACCAGTACC CACGAAGACC AAGCCGCCTC 
     2821 CCACATATGA ATCAGAAGAG GAGGATAAGT GTAAGCCCAT GTCTTATGAG GAGAAGCGGC 
     2881 AGCTAAGTCT AGATATCAAC AAACTTCCTG GTGAGAAGCT AGGCCGTGTA GTACACATAA 
     2941 TTCAGTCAAG GGAACCATCA CTTAAAAACT CCAACCCCGA TGAGATTGAG ATTGACTTTG 
     3001 AGACCCTGAA GCCATCTACA CTACGAGAGT TGGAGCGATA TGTCACCTCC TGTTTGCGGA 
     3061 AGAAAAGGAA ACCTCAAGCT GAAAAAGTTG ACGTGATTGC TGGTTCTTCC AAGATGAAGG 
     3121 GATTCTCATC CTCTGAGTCG GAGAGCACCA GCGAATCCAG CTCCTCTGAC AGTGAAGACT 
     3181 CTGAAACAGA GATGGCTCCC AAGTCAAAAA AGAAGGGGCA CACTGGGAGG GACCAGAAAA 
     3241 AGCATCATCA CCATCACCAT CCACAGATGC AGCCAGCCCC AGCTCCTGTG CCCCAGCAGC 
     3301 CGCCCCCACC TCCACAGCAG CCTCCTCCAC CCCCACCTCC GCAGCAGCAG CAGCAGCAAC 
     3361 CTCCACCCCC ACCACCTCCG CCCTCCATGC CACAGCAGAC TGCCCCAGCG ATGAAGTCCT 
     3421 CGCCCCCACC CTTCATCACT GCCCAGGTCC CCGTCCTGGA ACCACAGCTG CCAGGCAGTG 
     3481 TCTTTGACCC TATTGGCCAC TTCACCCAGC CCATCTTGCA CCTGCCGCAG CCGGAGCTGC 
     3541 CTCCTCACCT GCCCCAGCCA CCTGAGCACA GCACTCCACC CCATCTCAAC CAGCATGCTG 
     3601 TGGTCTCTCC TCCAGCTTTG CACAATGCGC TGCCCCAACA GCCATCTCGG CCCAGTAACC 
     3661 GAGCTGCTGC TCTGCCCCCA AAGCCTACCC GACCCCCAGC TGTGTCCCCT GCCCTGGCCC 
     3721 AGCCCCCCCT GCTCCCACAA CCACCAATGG CTCAGCCCCC CCAAGTGCTG CTGGAGGATG 
     3781 AAGAGCCACC TGCCCCACCC CTCACCTCCA TGCAGATGCA GTTGTACCTA CAGCAGCTGC 
     3841 AGAAGGTGCA GCCTCCCACA CCACTACTCC CTTCCGTGAA GGTGCAGTCC CAACCCCCAC 
     3901 CGCCTTTGCC GCCTCCACCC CACCCTTCTG TGCAGCAGCA GCAGCTCCAG CCACAGCCAC 
     3961 CGCCACCCCC ACCTCCTCAG CCACAGCCAC CACCCCAGCA ACAACACCAG CCTCCACCAC 
     4021 GACCAGTTCA CTTGCCATCC ATGCCCTTTT CTGCTCATAT TCAGCAGCCC CCACCACCCC 
     4081 CAGGACAGCA ACCTACTCAC CCACCCCCAG GACAGCAGCC CCCACCACCA CAGCCTGCCA 
     4141 AGCCCCAGCA AGTCATCCAG CATCACCCTT CCCCCCGGCA CCACAAGTCA GACCCCTACT 
     4201 CAGCTGGTCA TCTTCGTGAG GCTCCCTCTC CGCTTATGAT ACATTCCCCT CAGATGCCAC 
     4261 AGTTCCAGAG CCTGACCCAT CAGTCTCCTC CCCAGCAAAA CGTCCAGCCT AAGAAGCAGG 
     4321 TAAAGGGCAG GGCTGAGCCA CAGCCACCAG GGCCAGTCAT GGGCCAAGGC CAGGGATGCC 
     4381 CACCTGCCTC ACCGGCTGCC GTGCCTATGC TGTCCCAGGA GCTGCGACCA CCTTCAGTCG 
     4441 TCCAGCCCCA GCCCCTGGTG GTAGTAAAGG AGGAGAAGAT TCACTCACCA ATCATTCGCA 
     4501 GCGAGCCTTT CAGCACCTCA CTTCGACCAG AGCCCCCCAA GCACCCAGAG AACATCAAGG 
     4561 CCCCAGTCCA CCTGCCCCAG CGGCCTGAGA TGAAGCCTGT AGACATAGGG AGGCCTGTGA 
     4621 TCCGGCCTCC AGAGCAGAGT GCACCCCCAC CAGGGGCCCC TGACAAGGAC AAACAGAAGC 
     4681 AGGAGCCAAA AACACCAGTG GCACCCAAAA AGGACCTGAA AATTAAGAAC ATGGGCTCCT 
     4741 GGGCCAGCCT GGTACAGAAG CATCCGACCA CCCCATCCTC CACAGCCAAG TCCTCAAGTG 
     4801 ACAGCTTTGA GCATTTCCGC CGTGCTGCTC GGGAGAAGGA GGAGAGGGAA AAGGCCCTGA 
     4861 AGGCTCAGGC TGAGCATGCA GAGAAGGAGA AGGAGCGGCT GAGGCAGGAG CGCATGAGGA 
     4921 GCCGAGAGGA TGAAGATGCG CTGGAACAGG CACGTCGAGC CCATGAGGAG GCACGACGGC 
     4981 GCCAGGAGCA GCAGCAGCAG CAGCAGCAGC AGAGACAGGA GCAGCAGCAG CAGCAGCAGC 
     5041 AGGCAGCTGC TGTGGCCGCT GCCTCTGCCC CCCAGGCCCA GAGCTCCCAG CCCCAGTCTA 
     5101 TGCTGGACCA GCAGAGGGAG TTGGCCCGGA AACGAGAGCA GGAGCGGAGG CGCAGGGAGG 
     5161 CAATGGCAGC TACAATTGAC ATGAATTTCC AGAGTGATCT TTTGTCAATA TTTGAAGAAA 
     5221 ATCTTTTTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5281 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5341 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5401 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5461 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5521 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5581 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5641 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5701 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5761 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5821 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5881 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5941 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6001 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6061 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6121 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6181 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6241 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6301 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6361 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6421 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6481 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6541 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6601 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6661 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6721 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6781 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6841 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6901 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6961 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7021 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7081 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7141 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7201 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7261 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7321 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7381 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7441 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7501 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7561 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7621 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7681 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7741 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7801 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7861 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7921 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7981 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8041 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8101 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8161 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8221 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8281 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8341 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8401 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8461 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8521 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8581 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8641 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8701 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8761 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8821 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8881 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8941 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9001 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9061 CAAAAATTTA ACGCGAATTT T
//