LOCUS       MR224906                8466 bp    DNA     circular  SYN 
DEFINITION  Iqsec3 (Myc-DDK-tagged) - Mouse IQ motif and Sec7 domain 3 (Iqsec3)
ACCESSION   NM_001033354
VERSION     NM_001033354.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4623..4714 
                     /label="Myc-DDK"  
     misc_feature    4744..5329 
                     /label="PolyA signal"  
     misc_feature    4801..4820 
                     /label="Restriction Enzyme"  
     misc_feature    5605..6277 
                     /label="ColE1"  
     misc_feature    6707..7501 
                     /label="Kan/Neo"  
     misc_feature    7536..7838 
                     /label="SV40 ori"  
     misc_feature    7924..8401 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4605..4611 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4605 
                     /label="Iqsec3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGCCTG CTGGAGAACC CGGTGCGCGC CGTGCTCTAC CTCAAGGAGC 
     1081 TCACGGCCAT CGTGCAGAAC CAGCAAAGCC TCATCCATAC CCAGCGCCAG CGCATCGATG 
     1141 AGCTGGAGCG GCGTCTAGAC GAGCTGAGCG CGGAGAACCG CAGCCTGTGG GAACACCAGC 
     1201 AGTTGCTGCA AGCCCAGCCT CCGCCTGGGC TGGTTCCCCC GCCGCCGTCT GCCCCACTGC 
     1261 CTGCTCCTGC AGTCACTGCT CCGGCCGCCG CTGCGGCCCA GGAACCGCTT CAGGACCACG 
     1321 GACAGCTCAT ACCCGCTTCA CCGGAGCCGC CGCTTCAGCA CCACGGTCAG CTCCTGGCGC 
     1381 AGCCCCAACC TGCACCCAGC AGCAGGGTTC AGACTCCTCA GTCGCCCCAC CAGCACCCGG 
     1441 TGGCACCCGG GGCCATAGCT GACAAGGAGA AGGAGCGTCC CTCGAGTTGT TGTGCTGCCG 
     1501 CGGGAGCCCT GCTTCAACAC GCATCCCCCG CCGCCCTCGG CAAGGGCGTC CTGAGCAGGA 
     1561 GACCCGAGAA TGAGACTGTG CTACACCAAT TCTGCTGCCC TGCCGCTGAC ACCGAGCAGA 
     1621 AGCCTGCCTG CTCCGACCTG GCTTCCCAAA GTGATGGCTC CTGTGCCCAG GCCGGTGGGG 
     1681 GCATGGAGGA CTCCGTGGTG GCAGCGGTGG CAGCCGGCAG ACCCAGTGCC CATGCCCCGA 
     1741 AGGCTCAAGC CCCGGAGCTA CAGCAGGAGG AGGAGCGGCC AGGGGCAGTG GGCTCCCCAC 
     1801 GGGCTGGCCC CCTCCGCGCG GCCTCTCCTG GCCGGCAGCA GCCTGCCCTG GCGACGGCGC 
     1861 TCTGCTCCCA CACTCCTGCT GCCTCCGAAT ACGAACTCTC CCTTGACCTA AAGAATAAAC 
     1921 AGATTGAAAT GCTAGAACAC AAATACGGGG GCCACCTGGT ATCCCGACGC GCCGCGTGCA 
     1981 CCATTCAAAC CGCTTTTCGC CAGTACCAGC TCAGCAAGAA CTTTGAGAAG ATCCGTAACT 
     2041 CGCTCCTGGA GAGCCGCCTG CCTCGGCGGA TCTCACTGCG CAAGGTGAGG GCGCCCACAG 
     2101 CGGAGAGTCT GGTGGCAGAG AAGGCGCTCC TGGAGGGCTG TGGGCTCCTT GGGCTCCCGC 
     2161 TGGGGCGCTC GCCCTCGCTG CCGCCCACCT TCGCTGGCTC GCTCACCGAG CTGGAGGACT 
     2221 CCTTTACCGA GCAGGTGCAG TCCCTGGCCA AGTCCATCGA CGACGCCCTC AGCACCTGGA 
     2281 GCCTCAAGAC CATGTGCTCG CTGCAAGAGA GTGGCGCGTA CCAGCTCCAC CAGGCGCTGC 
     2341 ACCCGAGTGC GGGACAGCCA GGCCTGGAGA CCGAGGCGGC GGCGCGGGAG CCAGAGAGCG 
     2401 GCCCCGGGTC TGGAGATGAG GCCGGTGGTC TGCCTCAGGG TCACAGCGGT ACCCTCATGA 
     2461 TGGCTTTCCG GGACGTTACG GTGCAGATCG CTAACCAGAA CATCTCTGTT TCCTCCTCCA 
     2521 CCGCCCTGTC TGTGGCCAAC TGTCTGGGTG CACAGACCGC CCAGGCCACA GCCGAGCCTG 
     2581 CTGCCGCCCA AGCCGAACAA GAGGACACCG CGGATCAGGA GGTCTCGGAA GTCCCAGCCT 
     2641 CGGAACAGAT GGACCCACCC AGTGAGGACT CAGAGGCAGC GGAGAGCCGC GCCCAGAGCG 
     2701 CACAGGAACC CGCGGTGGCA CAGGCCGTGG TGGAGGAGGC GGTGGCTACA GAGGCAGAGG 
     2761 AGGAAGAGGA GGGGGCCAAA CAGGCAGGGA AGGGGGCAGA GGCCGAGGGA GGCGACAACT 
     2821 CTGAGCAGCT GAGCAGCAGC AGCGCTTCCA CCAAGTCTGC CAAATCCAGC TCAGAGGCTT 
     2881 CTGCCGCTGC CTCCAAGGAG GCCCTGCAGG CTGTGATCCT GAGCTTGCCC CGCTATCACT 
     2941 GCGAGAACCC AGCCAGCTGC AGGTCCCCCA CGCTCTCCAC AGACACCCTG CGCAAGCGGC 
     3001 TGTACCGCAT CGGCCTCAAC CTCTTCAACA TAAATCCGGA CAAGGGCATC CAGTTCCTGA 
     3061 TCTCACGTGG CTTCATCCCT GACACCCCCA TCGGTGTGGC CCACTTCCTC CTCCAGCGTA 
     3121 AAGGCCTCAG CCGCCAGATG ATAGGAGAGT TCCTGGGCAA CAGCAAGAAG CAGTTCAACC 
     3181 GGGACGTGCT GGACTGTGTG GTAGATGAGA TGGACTTTTC CAACATGGAG CTGGATGAAG 
     3241 CCCTGAGGAA GTTTCAAGCC CACATCCGTG TGCAGGGGGA AGCACAGAAG GTGGAGCGAC 
     3301 TCATAGAAGC TTTCAGCCAG CGCTACTGCA TGTGCAATCC TGAGGTGGTG CAACAGTTCC 
     3361 ACAACCCAGA CACCATCTTC ATCCTGGCCT TTGCCATCAT CCTTCTCAAC ACCGACATGT 
     3421 ACAGCCCCAA CATCAAGCCT GACCGGAAGA TGATGCTAGA AGACTTTATC CGGAACCTCC 
     3481 GAGGTGTGGA TGATGGTGCA GACATCCCCA GAGAACTGGT GGTAGGCATC TATGAGAGGA 
     3541 TCCAGCAAAA GGAGCTCAAA TCCAATGAGG ACCATGTCAC ATATGTCACC AAGGTGGAGA 
     3601 AGTCCATCGT GGGCATGAAG ACGGTGCTGT CAATGCCACA CCGTCGCCTG GTCTGCTGTA 
     3661 GCCGGCTCTT CGAGGTGACC GATGTGAACA AGCTACAGAA ACAAGCAGCA CACCAGAGAG 
     3721 AAGTGTTCCT CTTCAATGAT CTGCTTGTGA TTCTCAAGCT ATGCCCAAAG AAGAAGAGCT 
     3781 CCTTCACGTA CACCTTCTGC AAGGCAGTCG GCCTGTTAGG CATGCGCTTT CACCTCTTCG 
     3841 AGAACGAGTA TTACTCCCAT GGCATCACCC TGGCAACCCC ACTCTCGGGC TCCGAGAAGA 
     3901 AGCAGGTGCT GCACTTCTGT GCCCTGGGCT CCGACGAGAT GCAGAAGTTC GTGGAAGACC 
     3961 TGAAGGAGTC CATCGCTGAG GTGACAGAGC TGGAGCAGAT CCGGATAGAA TGGGAACTGG 
     4021 AGAAGCAGCA GGGCACAAAG ACTCTCTCCG TAAGGTCTGC TGGAGCCCAA GGGGACCCAC 
     4081 AGTCGAAGCA AGGATCACCC ACAGCCAAGA GGGAGGCGAT GGCAGGAGAG AAAGCGGCGG 
     4141 AGAGCTCGGG GGAGGTGTCA ATTCACAACA GGCTTCAAAC GTCCCAGCAC AGCCCCAAGT 
     4201 TGGGGGTCGA GAGGGGAGCG CCTGCACCAT CACCACCAAC ATCACCACCG CCGTTGCCGC 
     4261 CAGACCCACA GCCTAGCCCC CTGAGGGAGC AGCCCCCACC GCTGCCACTG CCACCACCCA 
     4321 CACCTCCAGG CACCCTGGTA CAGTGCCAGC AGATTGTCAA GGTCATTGTC CTGGACAAAC 
     4381 CCTGCTTGGC CCGCATGGAG CCCCTGCTGA GCCAGGCTCT GTCCTGTTAC GCCTCATCCT 
     4441 CCTCAGACTC CTGTGGCTCC ACACCCTTGC GTGGTCCAGG CTCCCCAGTT AAGGTCATCC 
     4501 ACCAGCCTCC ACTGCCCCCG CCCCCGCCCC CATACAACCA CCCTCATCAG TTCTGCCCCC 
     4561 CAGGCTCCAT GCTGCTCCGG CGCCGTTACT CCAGCGGCTC CAGGAGCCTG GTGACGCGTA 
     4621 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4681 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4741 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4801 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4861 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4921 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4981 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5041 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5101 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5161 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5221 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5281 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5341 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5401 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5461 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5521 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5581 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5641 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5701 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5761 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5821 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5881 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5941 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6001 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6061 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6241 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6301 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6361 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6421 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6481 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6541 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6601 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6661 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6721 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6781 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6841 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6901 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6961 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7021 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7081 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7141 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7201 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7261 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7321 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7381 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7441 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7501 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7561 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7621 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7681 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7921 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7981 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8041 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8101 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8161 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8221 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8281 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8341 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8401 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8461 AATTTT
//