LOCUS       MR224899                8244 bp    DNA     circular  SYN 
DEFINITION  Kcnu1 (Myc-DDK-tagged) - Mouse potassium channel, subfamily U, member 1 (Kcnu1)
ACCESSION   NM_008432
VERSION     NM_008432.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4401..4492 
                     /label="Myc-DDK"  
     misc_feature    4522..5107 
                     /label="PolyA signal"  
     misc_feature    4579..4598 
                     /label="Restriction Enzyme"  
     misc_feature    5383..6055 
                     /label="ColE1"  
     misc_feature    6485..7279 
                     /label="Kan/Neo"  
     misc_feature    7314..7616 
                     /label="SV40 ori"  
     misc_feature    7702..8179 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4383..4389 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4383 
                     /label="Kcnu1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCAAACA TTGCTAGACA GTTTAAATCA GAAGGAGTTG ACGGAAACGT 
     1081 CATGTACAAT CGAAATCCAG GCAGCGTTCA TTCTTTCCTC CTTGGCGACT TTCTTCGGGG 
     1141 GACTCATCAT CTTATTCCTT TTCAGAATAG CCTTGAAAAG CTCAAGAAGT TGGAAATACG 
     1201 TCAAGGGGCC AAGAGGACTC TTGGAACTAT TCTCATCACG TAGAATCGAG GCTAATCCTT 
     1261 TGAGGAAACT TTACTTTCAT GGAGTATTTC GTCAGCGCAT CGAAATGCTG CTTTCTGCAC 
     1321 AGACCGTCGT GGGGCAAGTG TTGGTGATCC TTGTCTTTGT ACTAAGCATC GGGTCTCTTG 
     1381 TGATCTATTT CATCAATTCA ATGGATCCTG TTCGAAGGTG TTCTTCATAT GAAGACAAAA 
     1441 TTGTCCATGT GGATTTGAGT TTCAACGCTT TCTTTAGCTT CTATTTTGGG TTGAGGTTTT 
     1501 GGGCAGCTGA AGACAAGATC AAGTTCTGGT TGGAGATGAA TTCAATTGTA GACATTTTTA 
     1561 CCATCCCGCC AACCTTTATT TCTTATTATT TGAAGAGTAA TTGGCTAGGT TTGAGATTTC 
     1621 TAAGAGCTCT GCGGTTGCTC GAACTCCCTA AAATCTTACA GATCCTACAA GTCATCAAGA 
     1681 CCAGCAATTC AGTGAAGCTT TCCAAACTGT TGTCAATAGT TATCAGTACC TGGTTCACGG 
     1741 CAGCAGGATT CCTTCACCTG GTGGAAAATT CTGGTGACCC CTGGCTCAAC GGAAGAAACT 
     1801 CACAGACTAT GTCATACTTT GAGTCTATTT ATCTGGTGAC AGCAACAATG TCAACTGTTG 
     1861 GCTTTGGGGA CGTGGTGGCC AAGACATCCC TAGGACGGAT TTTCATTGTT TTCTTCACCC 
     1921 TTGGGAGTTT GATACTATTT GCAAACTACA TTCCAGAAAT GGTGGAGCTC TTTTCTACCA 
     1981 GGAAGAAATA CACCAAGCCC TACGAAGCAG TCAAAGGAAA AAAGTTCATC GTGGTCTGTG 
     2041 GAAACATCAC AGTTGACAGT GTTACTGCTT TCCTGAGGAA TTTTCTCCAC TGGAAGTCCG 
     2101 GGGAAATCAA TATTGAGATC GTATTCCTTG GAGAGACTCT CCCTTGCTTG GAACTGGAGA 
     2161 CCTTACTGAA GTGCCACACA TCCTGTACCA ACTTCGTATG CGGCACCGCA CTGAAGTTCG 
     2221 AGGATCTGAA GCGAGTTGCA GTGGAGAACT CGGAGGCGTG CCTGATTCTA GCCAACCATT 
     2281 TCTGTAGTGA CTTACATGAC GAAGACAACT CAAACATTAT GAGGGTGCTC TCGATCAAGA 
     2341 ACTATTATCC ACAGACCAGA GTCATCATTC AGATACTTCA GTCTCAAAAC AAGGTTTTCC 
     2401 TGTCAAAAAT CCCCAACTGG GACTGGAGTG CTGGAGACAA TATCCTCTGC TTTGCAGAGC 
     2461 TAAAGCTCGG ATTTATCGCC CAAGGCTGCT TGGTGCCAGG GCTGTGCACC TTTCTCACGA 
     2521 CTCTGTTCAT TGAACAAAAC CAAAAGGTTT TTCCTAAACA TCCCTGGCAA AAACATTTCT 
     2581 TGAATGGCTT GAAGAACAAG ATTCTGACAC AGCGCCTCTC TAACGACTTC GTGGGGATGA 
     2641 CATTTCCCCA GGTCTCCCGG CTCTGCTTTG TGAAGCTAAA TCTCATGCTG ATCGCCATCC 
     2701 AACACAAGCC CTTCTTTCAC AGTTGTTGCA CTCTGATACT AAACCCATCA TCCCAAGTGA 
     2761 GGCTGAATAA GGACACCTTA GGGTTCTTCA TTGCGGACTC CTCCAAAGCC GTCAAAAGGG 
     2821 CTTTCTTTTA CTGTTCCAAC TGTCACAGCG ATGTGTGCAA TCCTGAGCTA ATTGGAAAGT 
     2881 GTAACTGTAA AATCAAGAGC CGACAACAAC TCATAGCACC GACCATCATG GTGATGAAAA 
     2941 GCAGCTTGAC CGATTTCACC ACTTCTTCAC ACATCCACGC TTCTATGTCA ACAGAAATTC 
     3001 ACACTTGTTT TTCAAGAGAA CAGCCTAGTT TGATCACCAT TACAACCAAC AGACCAACGA 
     3061 CAAACGACAC AGTGGATGAT ACCGACATGC TGGACAGCAG TGGCATGTTT CACTGGTGCA 
     3121 GAGCAATGCC CTTGGACAAG GTGGTTCTGA AACGAAGTGA GAAGGCAAAA CACGAGTTTC 
     3181 AGAACCACAT TGTAGTATGC GTGTTTGGAG ATGCCCAATG TACCCTGGTG GGGCTTCGGA 
     3241 ATTTCGTGAT GCCCCTGAGA GCCAGCAACT ACACCCGGCA GGAGCTGAAG GACATTGTTT 
     3301 TTATTGGGTC TCTGGAGTAC TTCCAGAGAG AATGGCGATT TCTCCGAAAC TTTCCCAAGA 
     3361 TACACATTAT GCCTGGATCT GCACTCTACA TGGGAGATCT GATTGCAGTC AATGTAGAGC 
     3421 AGTGCTCTAT GTGCGTCATC TTAGCCACAC CCTACAAGGC ACTGAGCAGC CAGATTCTGG 
     3481 TGGACACAGA GGCCATCATG GCCACCCTCA ACATCCAGTC CCTGCGGATC ACCAGTCCTA 
     3541 CTCCAGGGTC TTCAAAGTCA GAAGTAAAGC CATCATCTGC CTTTGATAGT AAAGAAAGGA 
     3601 AGCAAAGATA CAAACAGATC CCCATTCTCA CTGAACTGAA GAATCCCTCC AACATCCACT 
     3661 TTATTGAGCA GATGGGCGGA CTGGATGGAA TGCTCAAAGG GACTAGCTTG CATCTCAGCA 
     3721 CTTCTTTCTC CACCGGTGCT GTCTTTTCAG ACACCTTCTT GGATTCTCTC CTGGCCACGT 
     3781 CCTTCTACAA TTACCATGTC GTGGAATTAC TTCAGATGCT AGTGACTGGA GGCATAAGCT 
     3841 CTGAGATGGA ACACTATTTG GTTAAGGAGA AGCCCTATAA GACAACTGAC GACTATGAGG 
     3901 CAATCAAGTC TGGGAGGACG CGGTGTAAGC TGGGACTCCT CTCTTTAGAC CAAACCGTTC 
     3961 TATCAGGCAT TAATCCAAGA AAAACCTTTG GACAGCTGTT CTGTGGCTCA TTGGATAATT 
     4021 TCGGGATCCT ATGTGTCGGC TTATACCGTA TGATTGATGA AGAGGAACCC AGCCAAGAAC 
     4081 ACAAAAGGTT TGTGATCACC AGGCCATCCA ATGAGTGCCA CCTGCTGCCC TCAGATCTCG 
     4141 TGTTTTGTGC CATCCCTTTC AACACCACCT GTGGCAAATC AGACAGCAGT CCTTCAATTC 
     4201 AGGCTCAAAA CAACTCTACA AACGCGACGA CGCCATTGGC CCAGGGGTCG AATTTCTTCG 
     4261 ATTCGCACCA TGCCGACGAG TCCCACGATC TTTACCCAGT CGACGACACG GGAGAGAGGT 
     4321 GGTCTCAGCA CCACCACTCC CGAGTCTATC CTTTGGACAC GTTAGATGCC AGTGATATTG 
     4381 TTCAAGAAAA AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4441 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4501 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4561 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4621 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4681 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4741 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4801 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4861 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4921 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4981 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5041 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5101 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5161 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5221 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5281 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5341 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5401 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5461 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5521 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5581 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5641 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5701 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5761 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5821 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5881 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5941 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6001 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6061 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6121 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6181 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6241 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6301 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6361 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6421 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6481 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6541 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6601 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6661 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6721 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6781 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6841 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6901 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6961 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7021 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7081 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7141 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7201 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7261 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7321 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7381 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7441 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7501 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7561 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7681 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7741 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7801 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7861 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7921 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7981 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8041 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8101 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8161 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8221 TAACAAAAAT TTAACGCGAA TTTT
//