LOCUS       MR224878                8361 bp    DNA     circular  SYN 
DEFINITION  Aff4 (Myc-DDK-tagged) - Mouse AF4/FMR2 family, member 4 (Aff4)
ACCESSION   NM_033565
VERSION     NM_033565.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4518..4609 
                     /label="Myc-DDK"  
     misc_feature    4639..5224 
                     /label="PolyA signal"  
     misc_feature    4696..4715 
                     /label="Restriction Enzyme"  
     misc_feature    5500..6172 
                     /label="ColE1"  
     misc_feature    6602..7396 
                     /label="Kan/Neo"  
     misc_feature    7431..7733 
                     /label="SV40 ori"  
     misc_feature    7819..8296 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4500..4506 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4500 
                     /label="Aff4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCGTGAA GACCGGAATG TGCTGCGCAT GAAAGAAAGG GAAAGGCGGA 
     1081 ATCAGGAAAT TCAGCAGGGT GAAGACGCCT TCCCACCTAG TTCTCCTCTC TTTGCTGAGC 
     1141 CATACAAAGT CACTAGCAAA GAAGATAAGT TATCAAGTCG GATTCAGAGC ATGCTTGGAA 
     1201 ACTACGATGA AATGAAGGAT TACATAGGAG ACAGATCGAT ACCAAAGCTT GTTGCAATTC 
     1261 CCAAGCCTGC AGTACCAACG ACCACAGACG AGAAAGCAAA CCCAAATTTT TTTGAGCAGA 
     1321 GACATGGAGG ATCTCATCAG AGTAGCAAAT GGACTCCTGT AGGCCCCGCC CCCAGCACCT 
     1381 CTCAGTCTCA GAAACGCTCC TCTGCCCTGC AGAGTGGACA CAGCAGCCAA CGGAGTGGTG 
     1441 CAGGAGGGAG TGGCGCCAGC AGCAGTGGCC AGAGGCATGA CCGTGACTCC TACAGCAGTA 
     1501 GCAGGAAAAA AGGCCAGCAC GGCTCAGAGC ACTCCAAATC TCGATCCTCA AGCCCTGGAA 
     1561 AACCCCAGGC TGTTTCTTCA TTAAGTTCTA GTCATTCCAG GTCTCATGGG AATGATCACC 
     1621 ATAGCAAGGA ACATCAGCGC TCCAAATCAC CTCGGGACCC TGATGCCAAC TGGGATTCTC 
     1681 CTTCCCGCGG ACCTTTTTCA AGTGGGCAAC ATTCAAGTCA GTCTTTCCCA CCTTCTTTGA 
     1741 TGTCAAAATC TAGTTCAATG TTACAGAAAC CCACTGCCTA TGTACGGCCC ATGGATGGAC 
     1801 AGGAGTCTGT GGAACCAAAG CTGTCCTCTG AGCACTACAG CAGCCAGTCT CATGGTAATA 
     1861 GCATGACTGA GCTGAAGCCC AGCAGCAAAG CACATCTCAC CAAGCTGAAA ATTCCTTCCC 
     1921 GACCTTTGGA TGCATCGGTT TCTGGTGATG TAAGCTGTGT AGATGAAATA CTTAAAGAGA 
     1981 TGACGCATTC ATGGCCTCCC CCTCTAACGG CTATTCATAC ACCATGCAAA ACAGAACCTT 
     2041 CCAAATTCCC TTTTCCAACT AAGGAGTCGC AACAGTCTAA CTTTGGCCCT GGAGAACAAA 
     2101 AAAGATACAG TACTGCTAAA ACATCAAATG GACACCAGTC AAAATCTATG TTAAAAGATG 
     2161 ACTTAAAACT AAGCAGCAGT GAAGACAGTG ATGGGGAACA GGATTGTGAT AAGACAATGC 
     2221 CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAACAGTGAG GGAGCAGACA 
     2281 ACTCCAGGGA TGACTCGAGC AGCCACAGTG GATCTGAGAG CAGCTCTGGG TCTGACTCGG 
     2341 AGAGTGAGAG CAGCTCCAGT GACAGTGAGG CTAATGAGCC GTCGCAGAGC GCATCTCCTG 
     2401 AGCCTGAACC ACCACCAACA AACAAGTGGC AACTTGATAA TTGGTTGAAT AAAGTGAACC 
     2461 CACATAAAGT ATCCCCAGCT TCCTCTGTAG ACAGCAACAT CCCATCTTCT CAAGCCTACA 
     2521 AGAAAGAAGG CCGGGAGCAG GGCACTGCCA GTAACTACAC AGATCCAGGG GGGACAAAAG 
     2581 AAACGAGCTC TGCCACCCCA GGACGAGACT CTAAAACCAT CCAAAAGGGA TCAGAAAGTG 
     2641 GCCGTGGGAG GCAGAAGTCT CCTGCACAGA GCGACAGTAC CACTCAGAGG AGAACTGTCG 
     2701 GCAAAAAACA ACCCAAAAAA CCTGAGAAGT CAGCTGCTGA AGAGCCTCGT GGAGGCCTGA 
     2761 AGATAGAAAG TGAGACCCCT GTGGACATGG CTGCCAGCAT GCCCTCCAGC AGGCACAAAG 
     2821 CAGCCACCAA GGGCTCGAGG AAACCCAACA TCAAAAAGGA ATCAAAATCT TCTCCTCGAC 
     2881 CTACGGCAGA GAAGAAGAAG TACAAGTCAG CCAGTAAACC GTCCCAGAAG TCTCGGGAGA 
     2941 TCATAGAGAC TGACACCTCG TCCTCAGACT CGGACGGCAG CGAGAGCCTG CCTCCCTCCT 
     3001 CACAGACTCC TAAGTACCCT GAAAGCAACA GGACTCCTGT CAAACCCTCC TCAGTGGAGG 
     3061 AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG GAGCTCCTTT 
     3121 CCCCACTCAG TGAGCCTGAT GACAGGTATC CACTCATTGT GAAGATTGAC CTCAATCTTC 
     3181 TGACTCGAAT ACCCGGGAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG GAAAAGAAAA 
     3241 ATGTTCCAGA GAAACACTCG AGAGAGGTGC AGAAGCAAGC CTCAGAAAAG GCTTCCAACA 
     3301 AAGGCAAGAG GAAACACAAG AATGATGATG ATACCCGAGC CAGTGAGAGC AAAAAACCCA 
     3361 AAACAGAGGA CAAGAACTCA TCAGGCCACA AGCCATCCAG CAGCAGAGAG TCATCTAAGC 
     3421 AGAGTAGTAC AAAAGAAAAG GACTTGCTAC CTTCACCTGC TGGGCCCATT CTTTCAAAAG 
     3481 ATTCAAAAAC AGAGCATGGT TCTCGGAAGA GGACTGTCAG TCAGTCGTCG TCCTTAAAGT 
     3541 CCAGTGGGAC CAGCAGCAAA GAGAACAGTG GCAGCAGCAG CAAGAGCAGC TCCTCCTCCA 
     3601 CAGCGAAGCA GAAGAAGACT GAAGGCAAGG GTCCCAGCAG CTCCAAGGAG GCCAAGGAGA 
     3661 AGGCTCCAAA TAGCTCCTCT AATTGTCCTC CGTCTACACC AACTTCTGAG TCTTCGAAGC 
     3721 CTCGGAGAAC AAAGCTTGCC TTTGATGACA GAAATTATTC AGCAGACCAT TATTTACAAG 
     3781 AAGCAAAAAA ACTCAAACAC AATGCAGATG CTTTGTCTGA CAGGTTTGAG AAAGCCGTGT 
     3841 ACTACCTTGA TGCTGTAGTG TCTTTCATTG AGTGTGGGAA TGCATTAGAG AAGAACGCTC 
     3901 AGGAATCCAA GTCCCCATTC CCTATGTACT CAGACACGGT GGAGCTCATT AAGTACACTA 
     3961 TGAAACTAAA GAATTATTTG GCACCAGATG CTACAGCTGC AGACAAGAGA CTCACAGTAC 
     4021 TTTGCCTGAG ATGCCAGTCA TTATTGTACC TGAGGCTGTT CAAACTGAAG AAGGAGAACG 
     4081 CTCTCAAGTA CTCCAAGACG CTAACTGAGC ACCTGAAGAA TTCTTACAGT AATTCTCAAG 
     4141 CACCTTCACC CGGCTTGGGG AGCAAAGCTG TTGGGATGCC CTCCCCTGTT TCTCCAAAAC 
     4201 TGTCACCAGG CAATTCTGGA AGTTACTCTT CTGGGGGAAG TAGTGCTTCA GCAAGTGGTT 
     4261 CTTCAGTGAC CATTCCACAG AAAATCCACC AGATGGCAGC AAGCTATGTC CAGGTTACAT 
     4321 CTAACTTCCT CTATGCCACA GAAATTTGGG ACCAAGCTGA ACAGCTTTCC AAAGAACAAA 
     4381 AAGAATTCTT TGCTGAACTG GACAAAGTGA TGGGCCCTCT CATCTTTAAT GCAAGCATCA 
     4441 TGACAGATCT GGCTCGTTAC ACCCGGCAGG GACTGCATTG GCTTCGGCAG GATGCCAAGC 
     4501 TGATATCTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4561 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4621 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4681 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4741 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4801 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4861 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4921 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4981 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5041 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5101 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5161 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5221 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5281 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5341 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5401 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5461 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5521 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5581 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5641 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5701 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5761 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5821 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5881 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5941 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6001 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6061 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6121 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6181 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6241 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6301 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6361 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6421 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6481 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6541 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6601 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6661 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6721 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6781 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6841 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6901 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6961 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7021 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7081 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7141 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7201 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7261 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7321 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7381 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7441 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7501 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7561 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7801 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7861 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7921 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7981 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8041 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8101 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8161 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8221 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8281 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8341 CAAAAATTTA ACGCGAATTT T
//