LOCUS       MR224867                7206 bp    DNA     circular  SYN 
DEFINITION  Igsf1 (Myc-DDK-tagged) - Mouse immunoglobulin superfamily, member 1 (Igsf1), transcript variant 4
ACCESSION   NM_183336
VERSION     NM_183336.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3363..3454 
                     /label="Myc-DDK"  
     misc_feature    3484..4069 
                     /label="PolyA signal"  
     misc_feature    3541..3560 
                     /label="Restriction Enzyme"  
     misc_feature    4345..5017 
                     /label="ColE1"  
     misc_feature    5447..6241 
                     /label="Kan/Neo"  
     misc_feature    6276..6578 
                     /label="SV40 ori"  
     misc_feature    6664..7141 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3345..3351 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3345 
                     /label="Igsf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCTTCATA CTCATGGCCC TTCTTTGCTG TGGACTGTGC AATGGGGCAT 
     1081 TGACAGAGGA GATTGAAATA GTCATGCCAA CCCCTAAGCC TGAACTGTGG GCAGAGACGA 
     1141 ACTTCCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCA TCTGGCTCAA 
     1201 CTAAGGAGTT TGTGTTACTG AAGGACGGGA CCGGGTGGAT TGCAACCCGC CCGGCCTCAG 
     1261 AGCAGGTCCG GGCTGCCTTC CCTCTTGGCG CCCTGACCCA CAGCCACACC GGGAGTTACC 
     1321 ATTGCCATTC ATGGGAGGAG ATGGCTGTGT CGGAGCCTAG TGAAGCGCTT GAACTAGTGG 
     1381 GGACAGACAT CCTTCCCAAA CCTGTCATTT CGGCTTCCCT TCCAATCCGG GGCCAGGAAC 
     1441 TGCAGATCCG CTGTAAAGGA TGGCTGGAAG GTTTGGGGTT TGCTCTGTAT AAGAAGGGAG 
     1501 AGCAGGAACC TGTCCAGCAA CTTGGTGCTG TTGGGAGAGA AGCCTTCTTT ACAATTCAAA 
     1561 GAATGGAGGA TAAAGATGAG GGCAATTACA GCTGCCGCAC ACACACTGAA ATGCAGCCCT 
     1621 TCAAGTGGTC TGAGCCCAGT GAGCCCCTGG AGCTTGTCAT AAAAGAAATG TACCCCAAGC 
     1681 CTTTCTTCAA GACCTGGGCT AGCCCTGTGG TCACTCCTGG TTCCCGAGTG ACTTTCAATT 
     1741 GTTCCACCTC CCATGAACAC ATGAGCTTTA TTCTTTATAA GGATGGCAAT GAAATTGCAT 
     1801 CCAGTGACCT AGCCTGGGGA AACCCAGGAG GCAGTACAGC CCACTTTCTG ATCATTTCAG 
     1861 TGGGCATTGG TGATGGAGGA AACTATAGCT GTCGTTATTA TGACTTTTCC ATCTGGTCTG 
     1921 AGCCCAGCAA CCCTGTGGAG CTCGTTGTGA CAGAATTCTA CCCTAAACCC ACACTCCTGG 
     1981 CACAGCCAGG CCCTGTGGTG CTTCCTGGGA AGAATGTGAC CCTGCGCTGC CAAGGAATTT 
     2041 TCCAGGGCAT GAGGTTTGCC CTCTTGCAGG AGGGTACTCA TACCCCTTTA CAGTTCCAAA 
     2101 GTACCTCGGG GACCTCAGCT GACTTCCTCC TCCACACTGT TGGAGCACAG GACTTTGGGA 
     2161 ACTACAGCTG TGTCTACTAT GAGACAACCA TGTCGAACAG GGGCTCATCC CTTAGCACCC 
     2221 CTCTTATGAT CTGGGTGACT GATACATTCC CCAGACCATG GTTGTCTGCT GAGCCTAGTT 
     2281 CTGTGGTCAC CATGGGGCAG AATGTTACTC TATGGTGTCA AGGACCAGTC CGTGGAGTTG 
     2341 GGTACATTCT GCACAAAGAA GGAGAAGCCA CTTCGATGCA GCTTTGGGGG TCCACTAGCA 
     2401 ATGAAGGAGC ATTCCCTATC ATCAATATAT CTGGTGCTAG CATAGGACGT TATAGCTGCT 
     2461 GTTACCACCC TGACTGGATG AGCCCTATCA AGATACAGCC TAGCAACACT CTGGAACTCA 
     2521 TAGTCACTGG TTTACTCCCT AAGCCCAGCC TGTTAGTCCA GCCTGGTCCC ATGGTAGCCC 
     2581 CTGGAGAAAA CGTGACTCTT CAATGTCAAG GAGAACTGCC AGATTCAACA TTTGTCCTGT 
     2641 TGAAGGAGGG GACGCGACAG CCCTTAGAAC AACAGAGGCC AAGTGGGTAC AGAGCTGACT 
     2701 TCTGGATGCC AGTGGTGAGG GATCAAGATT CTGGTGTTTA CAGCTGTGTT TATTATCTGG 
     2761 ATTCTGCTCC CCTTGTGGCT TCGAATCACA GCAACTCTCT AGAGATCTGG GTGACTGATA 
     2821 AGCCCCCTAA ACCCTCTCTG TCAGCCTGGC CCAGCACCAT CTTCAAACTA GGCAAGGACA 
     2881 TCACCCTTCA GTGCCGAGGA CCCCTGCCAG GAGTTGAATT TGTGCTAGAG CATGATGGAG 
     2941 AAGAGGCTCC ACAACAGTTT TCCGAGGATG GAGATTTTGT CATCGACAAC TTGGAAGGGA 
     3001 AAGGCATTGG AAACTACAGT TGCAGCTACC GGCTGCAGGC CTACCCTGAT ATCTGGTCAG 
     3061 AGCCCAGCGA TACCCTGGAG CTGGTTGGAG CTGCAGGGCC TGTTGCTCAA GAGTGCACTG 
     3121 TGGGTAACAT TGTCCGAAGT ACCCTGATTG TGGTGGTTGT GGTAGCCTTG GGGATAGTGC 
     3181 TAGCCGTAGA GTGGAAGAAG TGGCCTCGAC TTCGGACTAG GGGCTCTGAG ACAGATGGAA 
     3241 GAGATCAGAC CGTAGTCCTT GAAGAGTGTA ACCAAGAAGG AGAACCAGGC ACTACCACCA 
     3301 ATTCTCCTTC ATCTGCCTCG CAAGAAGTCT CAGTGGAACT GACAGTTCCA ATAACGCGTA 
     3361 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3421 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3481 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3541 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3601 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3661 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3721 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3781 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3841 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3901 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3961 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4021 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4081 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4141 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4201 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4261 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4321 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4381 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4441 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4501 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4561 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4621 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4681 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4741 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4801 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4861 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4921 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4981 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5041 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5101 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5161 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5221 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5281 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5341 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5401 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5461 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5521 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5581 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5641 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5701 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5761 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5821 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5881 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5941 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6001 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6061 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6121 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6181 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6241 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6301 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6361 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6421 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6481 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6541 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6601 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6661 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6721 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6781 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6841 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6901 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6961 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7021 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7081 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7141 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7201 AATTTT
//