LOCUS       MR224865                7644 bp    DNA     circular  SYN 
DEFINITION  Whrn (Myc-DDK-tagged) - Mouse whirlin (Whrn), transcript variant 2
ACCESSION   NM_001008791
VERSION     NM_001008791.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3799..3890 
                     /label="Myc-DDK"  
     misc_feature    3920..4505 
                     /label="PolyA signal"  
     misc_feature    3977..3996 
                     /label="Restriction Enzyme"  
     misc_feature    4781..5453 
                     /label="ColE1"  
     misc_feature    5883..6677 
                     /label="Kan/Neo"  
     misc_feature    6712..7014 
                     /label="SV40 ori"  
     misc_feature    7100..7577 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3774..3781 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3774 
                     /label="Whrn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACGCACAG CTGGACGGCC TGTCGGTGAG CTCGTCCTCC ACCGGCTCGC 
     1081 TGGGCTCGGC GGCGGCGGCG GCGGGCGGCG GCGGGGGCGC GGGGTTGCGG CTGCTGTCTG 
     1141 CCAACGTGCG CCAGCTGCAC CAAGCGCTCA CGGCGCTGCT GAGCGAGCCC GAGCGGGAGC 
     1201 AGTTCACTCA CTGCCTCAAC GCGTACCACG CGCGCCGCAA CGTCTTCGAC CTGGTGCGCA 
     1261 CCCTGCGCGT CCTGCTGGAC AGCCCGGTCA AGCGGCGTCT GCTGCCCATG CTGCGTCTGG 
     1321 TCATCCCGCG CTCCGACCAG CTGCTCTTCG ACCAGTACAC GGCCGAGGGC CTCTACCTGC 
     1381 CAGCCACCAC CCCCTACAGG CAGCCCGCCT GGGCCGCTCC CGACGGCGCG GGGCCCGGGG 
     1441 AGGTGCGACT CGTGAGCCTG CGGCGCGCCA AGGCCCACGA GGGCTTGGGC TTCAGCATCC 
     1501 GCGGGGGCTC GGAACACGGC GTGGGCATCT ACGTGTCTCT AGTGGAGCCG GGCTCCCTGG 
     1561 CAGAGAAGGA AGGGTTGCGG GTCGGGGACC AGATTCTGCG CGTCAACGAT AAATCTCTAG 
     1621 CCCGGGTGAC CCACGCGGAG GCTGTCAAGG CTCTCAAAGG CTCCAAGAAG CTGGTGCTGT 
     1681 CTGTATACTC AGCTGGGCGT ATCCCAGGGG GCTATGTGAC CAACCACATC TACACCTGGG 
     1741 TGGACCCACA GGGTCGAAGC ACATCCCCTC CCTCCAGCCT GCCCCAGCCC CATGGCAGCA 
     1801 CCCTGAGACA GCGTGAAGAT GACCGAAGGA GTACCCTCCA CCTCCTGCAG AGTGGAGATG 
     1861 AGAAAAAGGT GAACCTGGTG TTGGGGGACG GCCGGTCCTT GGGCCTCACG ATCCGAGGTG 
     1921 GAGCAGAGTA CGGCCTTGGC ATTTACATCA CTGGTGTGGA CCCAGGCTCT GAAGCAGAGA 
     1981 GCAGCGGCCT CAAGGTTGGA GACCAGATTC TGGAGGTGAA TGGGCGGAGC TTTCTCAACA 
     2041 TCCTGCATGA TGAGGCAGTG AAGCTGCTCA AGTCATCCCG GCACCTCATC CTGACGGTGA 
     2101 AGGACGTCGG AAGGCTGCCC CACGCACGTA CCACCGTGGA CCAGACCAAG TGGATCGCCA 
     2161 GTTCCCGGAT CGGGGAAAGC GTCGCCAACT CAGCAGGGTT TCCAGGGGAC CACACAGAAG 
     2221 AAGGGACAAG CAAGCCAGGA TTTTACAAGG GCCCAGCCGG CTCCCAGGTG ACCCTGAGCA 
     2281 GCCTGGGGAA TCAGACTCGT GCACTGCTGG ACGACCAAGC CAGGCACCTG CTGACAGAGC 
     2341 AGGAGCGCGC CACCATGATG TACTACCTGG CCCAGTACCG TGGTGGCACC ATCTCTGTGG 
     2401 AGGCCATGGT CATGGCACTG TTTGAGTTGC TCAACACACA CGCCAAGTTC TCACTCCTGT 
     2461 CTGAGGTGAG AAGCATCATC TCACCCCAAG ACCTGGACCG CTTTGACCAC CTGGTGCTGA 
     2521 GGCGCGAGAT CGAGTCTATG AAGGCGAGGC AGCCCCCCGG CCCCGGGGTT GGAGACACGT 
     2581 ATTCCATGGT TTCCTACAGC GACACAGGCT CATCCACAGG CAGCCACGGC ACCTCTACAA 
     2641 CCGTCAGCTC GGCCAGGGAG CGGCTGCTGT GGCTTATAGA CCTGATGGAG AATACATTAG 
     2701 ACCTGGAAGG AACCGGTGAG ACCACCCAGG GCAGCACCAA CGCTCTCCCG GATGTGTCTG 
     2761 TGGATGATGT CAAATCCCCC TCAGAAGATC TGCCTGGTAT CAAGCCACCT CCTCCCCCAC 
     2821 CACCCCTGGC TCAAGGTCAT GACCGCTTGC TTGGCCAGCC ACGGAAGCCA GGGAGGGAGG 
     2881 ACCCCGCACC TCTGTCTTCT GCTGCCCACT CAGGCATTGT CTTCTCAGCA CCACGGAACC 
     2941 GCAGCCCGCC GCCAGGGACT GCTCCCACTC CGGGACCTTC CTCAGCACAG GACTCTCCCT 
     3001 CCTCCCCCAT TTATGCCTCC ATCTCCCATG CCAACCCCAG TTCCAGAAAG CCGCTGGACA 
     3061 CCCACCTGGC CCTGGTTAAC CAGCACCCCA TCGGCCCCTT CCCTCGAGTC CAGTCCCCAC 
     3121 CTCACCTGAA GAGCCCTCCT GCAGAGACCC CAGGAGCTGG GGCCTGCCTT CCACCACCAT 
     3181 CACCCTCTGA ACACCCTGAC GCCGTGGGTG CAAACCAGCA CTTTGTTCTG GTGGAGGTGC 
     3241 ACCGTCCGGA CAGTGAGCCT GATGTGAATG AAGTGCGGGC TCTGCCCCAG ACTCGCACAG 
     3301 CCTCCACACT CTCTCAGCTC TCAGACAGTG GGCAGACCCT GAGCGAGGAC AGTGGCGTGG 
     3361 ATGCCGGGGA GACGGAGGCC AGCACCTCAG GCCGAGGCAG ACAGACAGCA TCCGCTAAGA 
     3421 ACAAGAATGG CAAGGAGCAG CCCCGGACGG AGAGGACCGC GGAGGGTGCC AACAAACCTC 
     3481 CCGGCCTGCT GGAGCCAACG TCCACCCTGG TCCGTGTGAG GAAAAGTGCA GCCACACTGG 
     3541 GCATCGCCAT TGAGGGCGGT GCCAACACAC GCCAGCCTCT GCCCAGGATC GTCACAATTC 
     3601 AGCGAGGAGG TTCTGCCCAT AACTGTGGAC AGCTCAAGGT GGGCCACGTA ATTCTGGAAG 
     3661 TGAATGGGCA GACACTTCGG GGTAAGGAGC ACAAGGAGGC CGCCCGAATC ATCGCTGAGG 
     3721 CCTTCAAGAC CAAGGAGAGA GACTACATCG ACTTTCTGGT CACTGAGTTC AACGTGATGC 
     3781 TCAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3841 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3901 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3961 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4021 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4081 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4141 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4201 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4261 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4321 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4381 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4441 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4501 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4561 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4621 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4681 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4741 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4801 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4861 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4921 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4981 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5041 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5101 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5161 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5221 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5281 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5341 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5401 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5461 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5521 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5581 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5641 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5701 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5761 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5821 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5881 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5941 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6001 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6061 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6121 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6181 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6241 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6301 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6361 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6421 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6481 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6541 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6601 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6661 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6721 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6781 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6841 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6901 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6961 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7081 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7141 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7201 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7261 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7321 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7381 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7441 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7501 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7561 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7621 TAACAAAAAT TTAACGCGAA TTTT
//