LOCUS       MR224849                8676 bp    DNA     circular  SYN 
DEFINITION  Rasgrf1 (Myc-DDK-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1
ACCESSION   NM_011245
VERSION     NM_011245.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4833..4924 
                     /label="Myc-DDK"  
     misc_feature    4954..5539 
                     /label="PolyA signal"  
     misc_feature    5011..5030 
                     /label="Restriction Enzyme"  
     misc_feature    5815..6487 
                     /label="ColE1"  
     misc_feature    6917..7711 
                     /label="Kan/Neo"  
     misc_feature    7746..8048 
                     /label="SV40 ori"  
     misc_feature    8134..8611 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4815..4821 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4815 
                     /label="Rasgrf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG CAGAAAGCCA TCCGGCTTAA CGATGGCCAC GTCGTGACCC 
     1081 TGGGACTGCT GGCACAGAAG GACGGCACGC GCAAAGGCTA CCTGAGCAAG AGGAGTGCGG 
     1141 ACAACCCAAA ATGGCAAACC AAGTGGTTTG CCCTGCTCCA GAACCTGCTT TTCTACTTCG 
     1201 AAAGCGACTC CAGCCCTCGG CCCTCGGGGC TCTACCTGCT CGAGGGAAGT ATCTGTAAAC 
     1261 GCGCGCCCTC CCCCAAGCGA GGGACCTCCT CCAAGGAGTC CGGCGAGAAA CAGCAACATT 
     1321 ACTTCACAGT GAACTTCTCC AATGACAGCC AGAAGACCTT GGAGTTGAGG ACCGAGGATG 
     1381 CCAAGGACTG TGACGAGTGG GTGGCTGCGA TCGCTCGCGC CAGCTACAAG ATACTGGCTA 
     1441 CAGAACATGA GGCGCTTATG CAGAAGTACT TACACCTGCT GCAGGTAGTG GAGACAGAGA 
     1501 AGACTGTGGC TAAGCAGCTG CGCCAGCAGC TTGAGGACGG TGAGGTTGAG ATCGAGCGCC 
     1561 TGAAGACGGA GGTAACTATT ACAAACCTGA TCAAGGACAA TGATCGTATC CAATCCAGCA 
     1621 ACAAAGCCGG TTCTGCTGAT GATGAGGACA GTGACATCAA GAAAATTAAG AAGGTTCAGA 
     1681 GCTTCCTTCG GGGATGGCTG TGCCGGCGAA AGTGGAAGAA CATCATCCAG GACTACATCC 
     1741 GGTCTCCCCA CGCGGACAGC ATGCGCAAGA GGAACCAGGT GGTGTTCAGC ATGCTGGAGG 
     1801 CCGAGGCTGA GTACGTCCAG CAGCTACACA TCCTTGTCAA CAACTTCCTG CGCCCACTGC 
     1861 GCATGGCCGC CAGCTCTAAG AAACCCCCTA TAACACACGA CGACGTCAGC AGTATCTTTC 
     1921 TGAACAGCGA GACCATCATG TTCCTGCATC AGATCTTCTA CCAAGGCCTG AAGGCCCGTA 
     1981 TCTCCAGCTG GCCCACCCTG GTGCTGGCGG ACCTGTTCGA TATCCTGCTG CCCATGCTGA 
     2041 ACATCTACCA GGAGTTCGTC CGCAACCACC AGTACAGCCT CCAGATCCTT GCTCACTGCA 
     2101 AGCAAAACCG GGACTTTGAC AAGCTCCTCA AGCAGTACGA GGCCAAGCCA GACTGCGAGG 
     2161 AGCGCACACT GGAGACCTTC CTCACCTACC CAATGTTCCA GATCCCCAGG TACATCCTGA 
     2221 CACTCCATGA GCTGCTGGCC CACACACCTC ATGAGCATGT GGAGCGCAAC AGCCTGGACT 
     2281 ATGCCAAATC CAAACTAGAG GAACTGTCCA GGATCATGCA TGACGAAGTC AGTGAAACCG 
     2341 AGAACATCCG CAAAAACCTG GCCATTGAGC GTATGATCAC GGAGGGCTGT GAGATCCTCC 
     2401 TGGACACCAG CCAGACCTTC GTGCGCCAAG GTTCCCTCAT GCAGATGTCC TTGTCTGAAA 
     2461 AGAGCAAGTC CTCTAGGGGC CGCCTGGGGT CTCTGTCCAC TAAGAAAGAG GGTGAGCGCC 
     2521 AGTGTTTCCT ATTCTCCAAG CATCTCATTA TCTGCACCAG AGGCTCTGGT GGGAAACTGC 
     2581 ACCTAACCAA GAATGGAGTC ATATCCCTCA TTGACTGCAC CTTACTGGAT GAACCCGAAA 
     2641 ACCTGGACGA CGAAGCCAAA GGAGCCGGAC CAGAGATAGA ACACCTGGAG TTTAAGATCG 
     2701 GGGTGGAGCC AAAGGACTCC CTACCCTTCA CTGTCATCCT GGTAGCCTCA ACCAGGCAGG 
     2761 AGAAGGCAGC ATGGACCAGT GACATCATCC AGTGCGTGGA TAATATCCGC TGCAATGGGC 
     2821 TCATGATGAA TGCCTTTGAA GAAAATTCCA AGGTCACCGT GCCTCAGATG ATCAAGTCTG 
     2881 ACGCTTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG AATTCTTGCA 
     2941 AAGTGCTTCA GATCCGCTAT GCCAGCGTGG AGCGCCTGCT GGAGCGCCTG ACTGATCTTC 
     3001 GCTTCCTGAG CATTGACTTT CTCAACACCT TCCTGCATTC CTATCGCGTC TTCACCAATG 
     3061 CTATGGTGGT CCTGGACAAG CTGATCAATA TCTACAGAAA ACCCATGAGT GCGATTCCTG 
     3121 CCAGGTCCCT GGAACTCTTG TTTTCCAGCA GCCACAACGC CAAGCTTCTG TACGGAGATG 
     3181 CCCCTAAGTC CCCTCGCGCC AGCCGCAAGT TCTCCTCGCC ACCGCCCTTG GCCATCGGGA 
     3241 CGTCATCCCC CAGCCGCCGT CGGAAGCTGT CCCTCAACAT CCCCATCATC ACAGGCGGCA 
     3301 AGGCGCTGGA ACTGGCTTCC CTTGGGTGCA GCTCCGATAG CTACGCCAAC ATACACTCAC 
     3361 CCATATCTCC CTTCGGCAAA ACCACACTGG ACACTGGCAA GCTCTGTATG GCCAGCAGCT 
     3421 TACCCAAGAC TCCAGAGGAG ATTGATGTGC CTGCCACGAT TCCAGAGAAG CCTGGGGAGC 
     3481 TCTCAGCCTC CAGGAAGCAT AGCTCTGATG TCCTGAAAGA AGAGTCCGAG GATGACCAGA 
     3541 ACCACAGTGA TGAAGACAAC ACAGAAGTGT CACCAGTTAA ATCCCCACCA ACACCAAAAT 
     3601 CGTTCTTAAA CAGAACCATT ACAGAGTTTC CATTCTTTAA CTACAACAAT GGAATCCTGA 
     3661 TGACCACGTG TCGTGATCTG GTGGACAATA ACCGCAGCAC TCTCTCAGCT ACTTCTGCCT 
     3721 TTGCCATAGC GACTGCAGGA GCCAATGAAG GCCCCTCAAA CAAGGAGGTG TTTCGAAGGA 
     3781 TGTCTTTGGC CAACACAGGC TTTTCCTCTG ACCAGAGAAA TATCGACAAG GAGTTTGTGA 
     3841 TCCGCAGAGC TGCCACCAAT CGTGTGCTGA ATGTGCTCCG CCACTGGGTC ACCAAGCACA 
     3901 CCCAGGACTT TGATACCGAC GATACGCTCA AATACAGGGT GATCTGTTTC CTGGAGGAGG 
     3961 TCATGCATGA CCCAGACCTT CTGACCCAAG AGCGAAAGGC AGCAGCCAAC ATCATCAGGA 
     4021 CTCTGACCCT AGAAGAAACA ACTGAACAAC ATAGCATGCT GGAAGAAGTC ATACTGATGA 
     4081 CGGAGGGTGT GAAGACTGAG CCCTTCGAAA ACCACCCAGC CCTGGAGATA GCAGAGCAGC 
     4141 TGACCCTGCT GGATCACCTT GTCTTCAAGA GTATTCCTTA TGAAGAGTTC TTTGGCCAGG 
     4201 GCTGGATGAA GGCCGAGAAG TATGAAAGGA CACCTTACAT TATGAAAACC ACCAAACACT 
     4261 TCAACCATGT CAGTAACTTC ATTGCTTCAG AAATTATCCG AAACGAGGAC ATCAGCGCAA 
     4321 GGGCAAGCGC CATCGAGAAG TGGGTGGCTG TGGCCGACAT ATGTCGCTGC CTGCACAACT 
     4381 ACAATGCTGT GCTGGAGATC ACCTCCTCCA TCAACCGCAG CGCCATCTTC CGTCTCAAAA 
     4441 AGACATGGCT CAAAGTTTCT AAGCAGACGA AATCTTTGCT TGACAAGCTC CAAAAGCTTG 
     4501 TGTCATCAGA TGGCAGATTT AAGAACCTCA GAGAGTCTTT GAGAAATTGT GATCCACCCT 
     4561 GTGTCCCTTA CCTGGGGATG TATCTCACCG ACTTGGTGTT CATCGAGGAG GGGACACCCA 
     4621 ATTACACAGA GGACGGCCTG GTCAACTTCT CCAAGATGAG AATGATCTCC CATATTATCC 
     4681 GAGAGATTCG CCAGTTTCAG CAGACTACCT ACAAAATCGA CCCCCAGCCA AAGGTAATTC 
     4741 AGTACTTACT GGATGAATCT TTCATGTTGG ACGAAGAAAG CCTGTATGAG TCTTCGCTCC 
     4801 TAATCGAACC AAAACTCCCC ACAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4861 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4921 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4981 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5041 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5101 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5161 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5221 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5281 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5341 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5401 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5461 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5521 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5581 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5641 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5701 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5761 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5821 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5881 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5941 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6001 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6061 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6121 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6181 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6241 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6301 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6361 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6421 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6481 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6541 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6601 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6661 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6721 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6781 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6841 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6901 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6961 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7021 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7081 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7141 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7201 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7261 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7321 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7381 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7441 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7501 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7561 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7621 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7681 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7741 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7801 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7861 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7921 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8101 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8161 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8221 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8281 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8341 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8401 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8461 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8521 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8581 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8641 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//