LOCUS       MR224835                7098 bp    DNA     circular  SYN 
DEFINITION  Papola (Myc-DDK-tagged) - Mouse poly (A) polymerase alpha (Papola)
ACCESSION   NM_011112
VERSION     NM_011112.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3255..3346 
                     /label="Myc-DDK"  
     misc_feature    3376..3961 
                     /label="PolyA signal"  
     misc_feature    3433..3452 
                     /label="Restriction Enzyme"  
     misc_feature    4237..4909 
                     /label="ColE1"  
     misc_feature    5339..6133 
                     /label="Kan/Neo"  
     misc_feature    6168..6470 
                     /label="SV40 ori"  
     misc_feature    6556..7033 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3237..3243 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3237 
                     /label="Papola"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGTTTCCA GTTACAACGC AGGGATCACA ACAAACGCAG CCACCACAGA 
     1081 GGCACTATGG CATTACCTCT CCTATCAGCT TAGCGGCCCC CAAGGAGACT GACTGCCTAC 
     1141 TCACACAGAA GCTCATCGAG ACGCTGAAGC CCTTTGGGGT TTTTGAAGAA GAAGAGGAAC 
     1201 TGCAGCGCAG GATTTTAATT TTGGGAAAAT TAAATAACCT GGTGAAAGAA TGGATTCGAG 
     1261 AAATCAGTGA AAGCAAGAAT CTCCCACAAT CTGTAATTGA AAATGTTGGA GGGAAGATTT 
     1321 TTACATTTGG ATCTTACAGA CTAGGAGTCC ACACGAAAGG TGCTGATATT GATGCGTTGT 
     1381 GTGTTGCACC AAGACATGTT GATCGAAGTG ACTTTTTCAC CTCATTCTAT GATAAATTGA 
     1441 AATTACAAGA AGAAGTGAAA GATTTAAGAG CTGTTGAAGA GGCATTTGTA CCAGTTATCA 
     1501 AACTCTGTTT TGATGGAATA GAGATTGATA TTTTGTTTGC AAGATTAGCA CTGCAGACTA 
     1561 TTCCAGAAGA TTTGGACCTA CGAGATGACA GTCTGCTTAA AAACCTAGAT ATAAGATGCA 
     1621 TAAGAAGCCT TAATGGTTGC AGGGTAACCG ATGAAATTTT ACATCTAGTA CCAAACATTG 
     1681 ACAACTTCAG GTTAACTCTG AGAGCCATCA AACTGTGGGC CAAACGGCAC AACATCTATT 
     1741 CCAATATATT AGGTTTCCTC GGTGGTGTTT CCTGGGCTAT GCTAGTAGCA AGAACTTGCC 
     1801 AGCTTTATCC AAATGCAATA GCATCAACTC TTGTACATAA ATTTTTCTTG GTATTTTCTA 
     1861 AATGGGAATG GCCAAATCCA GTGCTATTGA AACAGCCTGA AGAATGCAAT CTTAATTTGC 
     1921 CTGTGTGGGA CCCAAGGGTA AACCCCAGTG ATAGGTACCA TCTTATGCCT ATAATTACAC 
     1981 CAGCATACCC ACAGCAGAAC TCCACGTACA ATGTGTCCGT TTCAACACGG ATGGTCATGG 
     2041 TTGAGGAGTT TAAACAAGGT CTTGCTATCA CAGATGAAAT TTTGCTGAGT AAGGCAGAGT 
     2101 GGTCCAAACT TTTTGAAGCT CCAAACTTCT TTCAGAAGTA CAAGCATTAT ATTGTACTTC 
     2161 TAGCAAGTGC GCCCACGGAA AAGCAGCGTC TGGAATGGGT GGGCTTGGTG GAATCAAAAA 
     2221 TCCGCATCCT GGTTGGAAGC TTGGAGAAGA ATGAGTTTAT TACACTGGCT CATGTGAATC 
     2281 CCCAGTCATT TCCAGCCCCC AAAGAAAGTC CTGACAGGGA AGAATTTCGC ACAATGTGGG 
     2341 TGATTGGGTT AGTGTTTAAA AAAACTGAAA ACTCTGAAAA TCTCAGTGTC GACCTCACCT 
     2401 ATGATATCCA GTCTTTCACA GACACAGTTT ATAGGCAAGC AATAAACAGC AAAATGTTTG 
     2461 AGTTGGATAT GAAGATTGCA GCAATGCATG TGAAGAGAAA GCAACTCCAT CAGCTGCTGC 
     2521 CTAGTCACGT GCTTCAGAAG AGGAAGAAGC ATTCAACAGA AGGAGTCAAG TTAACAGCTC 
     2581 TGAATGACAG CAGCCTTGAC TTGTCTATGG ACAGTGATAA CAGCATGTCT GTGCCTTCAC 
     2641 CCACCAGTGC TATGAAGACC AGTCCATTGA ATAGTTCTGG CAGCTCCCAG GGCAGAAACA 
     2701 GTCCTGCTCC AGCTGTGACC GCAGCATCTG TGACCAGCAT CCAGGCTTCT GAGGTTTCTG 
     2761 TACCGCAAGC AAATTCCAGT GAAAGCCCAG GGGGTCCATC GAGCGAAAGC ATTCCTCAAA 
     2821 CTGCCACACA GCCAGCCATT TCTCCACCAC CAAAGCCTAC AGTCTCCAGA GTTGTCTCCT 
     2881 CAACACGACT GGTAAACCCA TCGCCTAGAC CTTCAGGAAA CACAGCAACA AAAGTCCCTA 
     2941 ATCCTATAGT AGGAGTCAAG AGAACGTCCT CACCCAATAA AGAAGAAAGT CCTAAGAAAA 
     3001 CCAAAACAGA AGAGGATGAA ACAAGTGAAG ATGCTAACTG TCTTGCTTTG AGTGGACATG 
     3061 ATAAAACAGA GACAAAGGAA CAAGTTGATC TGGAGACAAG TGCGGTTCAA TCAGAAACTG 
     3121 TTCCGGCATC GGCTTCTCTG TTGGCCTCTC AGAAAACATC CAGTACAGAC CTTTCTGATA 
     3181 TCCCTGCTCT CCCTGCAAAT CCTATTCCTG TTATCAAGAA TTCAATAAAA CTGAGACTGA 
     3241 ATCGGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3301 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3361 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3421 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3481 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3541 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3601 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3661 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3721 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3781 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3841 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3901 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3961 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4021 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4081 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4141 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4201 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4261 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4321 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4381 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4441 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4501 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4561 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4621 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4681 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4741 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4801 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4861 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4921 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     4981 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5041 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5101 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5161 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5221 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5281 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5341 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5401 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5461 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5521 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5581 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5641 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5701 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5761 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5821 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5881 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     5941 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6001 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6061 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6121 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6181 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6241 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6301 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6361 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6421 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6481 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6541 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6601 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6661 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6721 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6781 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6841 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6901 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     6961 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7021 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7081 AAATTTAACG CGAATTTT
//