LOCUS       MR224832                6675 bp    DNA     circular  SYN 
DEFINITION  Arsj (Myc-DDK-tagged) - Mouse arylsulfatase J (Arsj)
ACCESSION   NM_173451
VERSION     NM_173451.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2832..2923 
                     /label="Myc-DDK"  
     misc_feature    2953..3538 
                     /label="PolyA signal"  
     misc_feature    3010..3029 
                     /label="Restriction Enzyme"  
     misc_feature    3814..4486 
                     /label="ColE1"  
     misc_feature    4916..5710 
                     /label="Kan/Neo"  
     misc_feature    5745..6047 
                     /label="SV40 ori"  
     misc_feature    6133..6610 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2814..2820 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2814 
                     /label="Arsj"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCAGG GACAGTGCGG AGCCTCTGCC TCCGCTATCT CCACAGGCCT 
     1081 GGGCCTGGTC TGGAAAGTTC TTAGCGATGG GTGCCCTGGC AGGCTTCTCG GTCCTCAGCC 
     1141 TGCTCACCTA TGGCTACCTG TGCTGGGGCC AGGACTTAGA AGAGGAGGGG TCCCTAAAAG 
     1201 CTCAAGTTGA TGAGAGACCT GAAGCAGGCA CAGCAGGCAC CTCCCAGCCC CATCTCATTT 
     1261 TTATCTTAGC TGATGATCAG GGATTTAGAG ATGTGGGTTA CCACGGATCT GAGATAAAGA 
     1321 CGCCCACCCT TGATAAACTC GCTGCAGAAG GAGTTAAGCT AGAGAACTAC TATGTCCAGC 
     1381 CTATTTGCAC ACCGTCCAGG AGCCAGTTTA TTACCGGAAA GTATCAGATA CACACGGGAC 
     1441 TTCAGCATTC TATCATTAGA CCTACCCAAC CCAACTGTCT ACCTCTGGAC AATGCAACCC 
     1501 TACCTCAGAA GCTAAAGGAG GTCGGCTATT CAACCCATAT GGTTGGGAAG TGGCACTTGG 
     1561 GATTTTACAG AAAAGACTGC ATGCCCACCA AGAGAGGATT TGATACCTTT TTTGGCTCTC 
     1621 TTTTGGGAAG TGGTGATTAT TATACACACT ACAAATGTGA CAGTCCTGGG GTGTGTGGCT 
     1681 ATGACTTGTA CGAAAACGAC AATGCAGCTT GGGACTATGA CAATGGCATC TATTCAACAC 
     1741 AGATGTACAC GCAGAGAGTG CAGCAAATCT TAGCCACCCA TGATCCCACA AAACCTCTAT 
     1801 TTTTATATGT GGCTTACCAA GCTGTCCACT CACCACTTCA AGCCCCTGGC AGGTACTTTG 
     1861 AACACTACAG ATCCATTATC AACATAAACA GGAGGAGGTA CGCAGCCATG CTCTCCTGCT 
     1921 TGGATGAAGC AATCCACAAT GTGACCCTGG CCCTAAAGAG GTATGGTTTC TATAACAATA 
     1981 GCATTATCAT TTACTCCTCA GATAATGGTG GGCAGCCCAC AGCAGGAGGA AGTAACTGGC 
     2041 CACTCAGAGG CAGCAAAGGA ACATATTGGG AAGGAGGCAT CCGGGCAGTT GGCTTTGTGC 
     2101 ATAGCCCACT TCTAAAAAAC AAGGGAACGG TATGTAAGGA ACTTGTGCAC ATCACCGATT 
     2161 GGTACCCAAC CTTGATTTCA CTGGCTGAAG GACAGATTGA TGAAGACATT CAGCTAGATG 
     2221 GATACGATAT CTGGGAGACC ATAAGCGAAG GTCTTCGTTC ACCCCGAGTG GATATTTTGC 
     2281 ACAACATTGA CCCCATTTAC ACCAAGGCGA AAAATGGCTC CTGGGCAGCA GGCTATGGGA 
     2341 TCTGGAACAC TGCAATCCAG TCGGCCATCC GGGTACAACA CTGGAAACTG CTCACAGGCA 
     2401 ATCCTGGCTA CAGTGACTGG GTCCCCCCTC AGGCTTTCAG CAATCTGGGC CCAAACCGGT 
     2461 GGCACAATGA AAGGATTACC TTGTCAACTG GCAAGAGTAT CTGGCTTTTC AACATCACGG 
     2521 CTGATCCGTA TGAGAGGGTG GACCTATCCA GCAGGTATCC AGGCATCGTG AAGAAGCTGC 
     2581 TGCGGAGACT GTCACAATTC AACAAGACTG CCGTGCCTGT CAGGTACCCC CCGAAGGATC 
     2641 CCAGAAGCAA CCCTAGGCTC AACGGAGGAG TCTGGGGTCC GTGGTATAAA GAGGAAAACA 
     2701 AGAAAAAGAA GTCAAACAAA ACCAAGGCTA AGAAAATGCA AAAGAAAAAA AGTAAGGCAA 
     2761 GGATGAGGAA GCAGTTGGCA GCTCACTCCT CAATAAAATG CCACCCAAGT GTTGCCACTG 
     2821 GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     2881 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     2941 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3001 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3061 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3121 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3181 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3241 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3301 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3361 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3421 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3481 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3541 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     3601 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     3661 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     3721 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     3781 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     3841 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     3901 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     3961 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4021 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4081 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4141 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4201 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4261 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4321 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4381 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4441 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     4501 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     4561 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     4621 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     4681 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     4741 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     4801 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     4861 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     4921 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     4981 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5041 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5101 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5161 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5221 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5281 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5341 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5401 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     5461 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     5521 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     5581 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     5641 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     5701 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     5761 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     5821 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     5881 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     5941 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6001 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6061 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6121 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6181 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6241 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6301 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6361 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6421 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     6481 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     6541 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     6601 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     6661 TTTAACGCGA ATTTT
//