LOCUS       MR224827                8148 bp    DNA     circular  SYN 
DEFINITION  Uspl1 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase like 1 (Uspl1), transcript variant 1
ACCESSION   NM_001115153
VERSION     NM_001115153.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4305..4396 
                     /label="Myc-DDK"  
     misc_feature    4426..5011 
                     /label="PolyA signal"  
     misc_feature    4483..4502 
                     /label="Restriction Enzyme"  
     misc_feature    5287..5959 
                     /label="ColE1"  
     misc_feature    6389..7183 
                     /label="Kan/Neo"  
     misc_feature    7218..7520 
                     /label="SV40 ori"  
     misc_feature    7606..8083 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4287..4293 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4287 
                     /label="Uspl1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGATGAT TCTCTGAAGA TTGGAAATGG TTTGCCACTG GTTGGACCAG 
     1081 GGACTGATGT AGGGATATCT TCACTCCCCA TGCTGGGGTA CTTGGGAAAA AATTATGCTT 
     1141 CAGCTAAAGT CACAACAGAC GGGCATTGCC CTGCTTGTAG AGCGAAAGGC AAGCTAAGTG 
     1201 CCTTAAAACC TTACCGCATT AGCTTCCAAG AATCTGTCTT TCTGTGTGAG GATCTTCAGT 
     1261 GCATTTATCC TTTGGGCTCT GAGTCACTTA CTAATTTAAT TTCTCCTGAT TCAGAAGATT 
     1321 GTCCCACTCC AAGTAAGCCT CAAAAACGAA AAAGATTGGA AACCAACTGT AGGAATTCAC 
     1381 CTCTTCCAGT ACATTCTAAA AAGACTAGAA GTCACATTGT CACTGATAGT GAACCAATTG 
     1441 TGAATGGTAA ATATAATGGA GAAGTGTGTG ATGACTTCTC AGCAAGCTTC CCAGATACCA 
     1501 GTGCTCACCA GGATCCTGCC AGCACAGCTG CTTCTGTGGA GCAGAGTGAG GCTTTGGAGG 
     1561 CTGATGATGT GGTTGTGGCC GCTACAGAAG ATCCTGCTAC AGTTAGTGTC ACATCTGAGC 
     1621 TGGAAATGCC AGCTAAGAGC AGATGTTTAC CACTTTGCCA GACTCTGTGT GTCCAGTGGA 
     1681 AAAACACCCA GGCCCTGTGC TGGTTAGACT GTATCCTGTC AGCCCTGGTG CACTTGGAGG 
     1741 TGTTGAGGAA GACTGTGCTG GAGGCGTGCT CCAGGGAGGA GTGTGTATTT GGAAGGCTGT 
     1801 TTGAAATGTA CCACCAAGCA GATGAGCTGC TGCACACCCA TCACCTGCAT GGCGTCACAG 
     1861 GTGAAGATTG TAAAAAATTG ACATCAGAAA TATTTACAGA AATAGATACC TGTTTGAATA 
     1921 AAGTTAGAGA TGAAATTTTT GCTAAACTTC AACCGAAGCT TAGATGCACA TTAGGTGACA 
     1981 TGGAAAGTCC TGTGTTTGCA CTTCCTGTAC TGTTAAAGCT TGAACCCCAT GTTGAAAGCC 
     2041 TCTTTACATA TTCTTTTTCT TGGAATTTTG AATGTTCCCA TTGTGGACAC CAGTACCAAA 
     2101 ACAGGTGTGT GAAGAGTCTG GTCACCTTTA CCAATATTGT TCCTGAGTGG CATCCACTCA 
     2161 ATGCTGCCCA TTTTGGTCCA TGTAACAGCT GCAACAGTAA ATCACAAATA AGAAAAATGG 
     2221 TGTTGGAAAG AGCGTCGCCC ATACTCATGC TGCACTTCGT GGAGGGCTTA CCAAGGAGGG 
     2281 ATTTGCAACA CTATGCCTTC CACTTTGAAG GAAGCCTTTA TCAAGTCACG TCGGTAATAC 
     2341 AGTACCAAGC AAATAACCAT TTTATAACAT GGTTTTTAGA TGCTGATGGG AGTTGGCTGG 
     2401 AGTGTGATGA CCTAAAGGGT CCCTGTGCTA AAAGGCACGT GACCTGTGAA GTGCCAGCTT 
     2461 CAGAGACACA TATCGTTATT TGGGAAAGAA AATCCCAGGT GCCAATTGAA GAAGCTGCCT 
     2521 GCCTTCCATG CATGAAGCCA AATGTGCAGC CTGTATCAGG TGAGGAGCAG CCGACTTGTC 
     2581 CAGCACTGTG TTCTCTGGCT GGGACCGCCA CCTCAGAACC CTCAGTCGCC CATCCCACAT 
     2641 CTATGGCAGG CGCTCCTCAG ACTCTTCCAG AAATCCAAGC TGTAGCTCAT GGAGATAGTG 
     2701 TACTTTCAGG AGCAAAAGGC ATGGTCGACA GCATTTTACC CTCAGCACTT GAAGAAACCA 
     2761 TCCAGGAAAC TGCCTCTGTT TCTCAGGTTG ACTCCAAAGA TTGCCTGTTG GAAGACAAAC 
     2821 CCGTGGCAGG AAGTGCAGCA CTGGTCAGAG TACTTGCTTT TCAGCCACAG GACTCCCCGG 
     2881 GGTCTTCGGG GTCTTCCTTA GTGTCCAGCC TGTGTGAAGG AAAGCTAGTT GCCCCATGTG 
     2941 TGGATTCAAG TTTCCCATCC CAAGCTGTGA GCACAGACCT GCAGGCAGTA CTGTCCCAGG 
     3001 CAGGGGACAC TGTGGTTCCT AACCCTGTGA CTGATGCCCC TGTTCCTGTT CTTGTTCAGG 
     3061 AATTGAAGTC CCTGGCAACT GAGAAGGACT CTCAGACACA GTTGCTGCCA CTGAAAACTG 
     3121 AGAAGTTAGA TCCTGAGCAA CCTGGTAAAT CTCAGGCATC TAATTTGAGA AAACGAGAAA 
     3181 CCACAGCGTC TTCTAAAACA GTAGCAGCCA GGTCAGCACA GAATCAGCCT CGGAAAGAAG 
     3241 ATCAGAAGAG AGCCTTCGTG GGAAGTTGGG TGAAGGGGCT ATTGAGCAGG GGTGGTGCTT 
     3301 TTATGCCAAC CTGTGTTTTG TCTCAGAGTA GAGCAGTAAG TGACCTGCAA CCTTCTGTTA 
     3361 AAGGAGCCAG TAATTTTGAT GGCTTTAAAA CGAAAAGTAT AAGCCGCAGG TCTAAAAGAA 
     3421 TGTCCAGGAA AGCTAAGCAC ATGGAGGAAC TGTCTCCAAG GAACAGCTCT CCACCATTGA 
     3481 GCTGGACAGC TGCTCTGACC CAGGCTGCTG AGAACGCCAC CTCAGCTCTG TTGAGGGAGC 
     3541 AGGAAGGCTC ACGTCCAGCT CCCCTCAGGC ACAGATCTCC TGGCAATGAA AGCGCCATCT 
     3601 CTCCAGCAAG CCGTGGAGAT GCGGCTGAAG ATCAGGTTCA TAAGCTTCGG CTCAAACTTC 
     3661 TTAAAAAATT AAAGGCAAAA AAGAAGAAAC TAGCTGCCCT CATTTCTTCC CCGCACCGTG 
     3721 AACCATCTCT AAGTGACCAC TCAGAGCCTG CGTCCCATTG TGGAACACCT GCAAGTGACC 
     3781 AGTCAGAGCC TGTATCCCAT TGTGGATCTC CAAATGACTG TGAGTCCATA GAAGATCTGT 
     3841 TAAAAGAGCT ACAGCATCAA ATTGACCTTG CAGACAGCAA ATCTGGGTGC ACCTCTGCTC 
     3901 CTGATGCCAC CTCAAATAAC AGCCAGAGTC ATGAGGAGAT TTTAGCAGAG TTGCTGTCGC 
     3961 CTACAGCCAT GTCAGAGCCC TCAGAAAGTG GGGAGCTTGA GTTGAGGTAC TTGGAAATGG 
     4021 GGGACAGCAC CCCAGCACAA GCACCTAGTG AATTCAGTGT TGTCTCCCAG AACACATGTC 
     4081 TGAAACAGGA CCATGATTAC TGCAGCCCTG AGAAAGGCCA GCGGGAAGTT GATCTGCATT 
     4141 CAGTCATGGA CAGTGCCTGT ATAAGAACCT TGAACTTGGG GAGTCCCATG AAGACTGATA 
     4201 TCTTTGATGA TTTTTTTTCC ACCTCAGCAC TGAATTCTTT AACAAACGAC ACATTAGACA 
     4261 TACCTCATTT TGATGACTCT CTGTTTGAGA ATTGCACGCG TACGCGGCCG CTCGAGCAGA 
     4321 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4381 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4441 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4501 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4561 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4621 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4681 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4741 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4801 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4861 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4921 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4981 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5041 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5101 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5161 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5221 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5281 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5341 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5401 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5461 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5521 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5581 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5641 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5701 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5761 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5821 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5881 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5941 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6001 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6061 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6121 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6181 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6241 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6301 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6361 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6421 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6481 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6541 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6601 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6661 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6721 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6781 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6841 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6901 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6961 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7021 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7081 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7141 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7201 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7261 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7321 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7381 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7441 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7501 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7561 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7621 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7681 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7741 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7801 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7861 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7921 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7981 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8041 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8101 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//