LOCUS       MR224723                8913 bp    DNA     circular  SYN 
DEFINITION  Mink1 (Myc-DDK-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 4
ACCESSION   NM_001045964
VERSION     NM_001045964.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5070..5161 
                     /label="Myc-DDK"  
     misc_feature    5191..5776 
                     /label="PolyA signal"  
     misc_feature    5248..5267 
                     /label="Restriction Enzyme"  
     misc_feature    6052..6724 
                     /label="ColE1"  
     misc_feature    7154..7948 
                     /label="Kan/Neo"  
     misc_feature    7983..8285 
                     /label="SV40 ori"  
     misc_feature    8371..8848 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5052..5058 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5052 
                     /label="Mink1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACCCA GCCCCCGCCC GCAGCCTGGA CGACATCGAC CTGTCTGCCC 
     1081 TGCGGGACCC TGCAGGAATC TTTGAGCTGG TGGAGGTGGT TGGCAATGGA ACCTATGGAC 
     1141 AGGTATACAA GGGGCGGCAC GTCAAGACTG GGCAGCTGGC TGCCATTAAG GTCATGGATG 
     1201 TCACAGAGGA TGAGGAGGAA GAGATCAAAC AGGAAATCAA CATGTTAAAG AAGTACTCTC 
     1261 ACCATCGCAA TATTGCCACC TACTATGGGG CCTTTATCAA GAAGAGCCCT CCTGGGAACG 
     1321 ATGACCAGCT CTGGCTGGTG ATGGAGTTCT GCGGTGCTGG TTCAGTGACC GACCTGGTAA 
     1381 AGAACACAAA AGGGAACGCA CTGAAGGAGG ATTGCATTGC TTACATCTGC AGGGAGATTC 
     1441 TCAGGGGTCT TGCCCATCTC CATGCCCACA AGGTGATCCA CAGAGATATC AAGGGACAAA 
     1501 ATGTGCTGCT GACAGAGAAT GCTGAAGTCA AGCTAGTGGA TTTTGGGGTG AGTGCTCAGC 
     1561 TGGACCGCAC TGTGGGCAGG CGGAACACTT TCATTGGAAC CCCATACTGG ATGGCTCCAG 
     1621 AAGTCATTGC CTGTGACGAG AACCCCGATG CCACCTATGA CTACAGGAGT GACATTTGGT 
     1681 CTCTAGGAAT CACAGCCATT GAAATGGCAG AGGGAGCCCC CCCTCTGTGT GACATGCACC 
     1741 CTATGCGGGC CCTCTTCCTC ATCCCTCGGA ACCCTCCCCC CAGGCTCAAG TCAAAGAAAT 
     1801 GGTCTAAGAA GTTCACTGAC TTCATCGACA CGTGTCTCAT CAAGACTTAC CTGAGCCGCC 
     1861 CACCCACCGA ACAGTTACTC AAATTCCCCT TCATCCGAGA CCAGCCCACG GAGCGGCAGG 
     1921 TCCGCATCCA GCTCAAGGAC CACATCGACC GCTCGCGGAA GAAGCGGGGT GAGAAAGAGG 
     1981 AGACAGAGTA TGAGTACAGC GGCAGTGAGG AGGAAGACGA CAGCCATGGA GAGGAAGGCG 
     2041 AGCCAAGCTC CATCATGAAT GTGCCCGGTG AGTCCACACT GCGCAGAGAA TTCCTCAGAC 
     2101 TCCAGCAGGA GAATAAGAGC AACTCTGAGG CTTTAAAGCA GCAGCAGCAG CTGCAGCAAC 
     2161 AGCAGCAGCG GGACCCGGAG GCACACATCA AACACCTGCT GCACCAGCGG CAGCGTCGCA 
     2221 TAGAGGAGCA GAAGGAGGAG CGGCGACGTG TGGAGGAGCA ACAGCGGCGA GAGCGAGAAC 
     2281 AGCGTAAGCT ACAAGAGAAG GAGCAGCAGC GGCGATTGGA AGACATGCAA GCCCTACGAC 
     2341 GAGAGGAAGA GAGGCGGCAA GCAGAGCGGG AACAGGAATA CAAGCGGAAG CAGCTGGAGG 
     2401 AGCAGCGGCA GTCAGAGCGG CTGCAGAGAC AGCTGCAGCA GGAGCACGCC TACCTCAAGT 
     2461 CCCTGCAGCA GCAGCAGCAG CAGCAGCAGC TCCAGAAGCA GCAGCAGCAG CAGCAGCAGA 
     2521 TCCTGCCTGG AGACAGGAAG CCCCTGTATC ATTACGGTCG GGGCATTAAT CCTGCTGACA 
     2581 AGCCAGCATG GGCCCGCGAG GTGGAAGAGA GAGCACGGAT GAACAAGCAG CAGAACTCTC 
     2641 CCTTGGCGAA GGCGAAGCCA AGCAGTGCGG GGCCAGAGCC CCCCATCTCC CAGGCCTCTC 
     2701 CTAGCCCCCC AGGACCTCTT TCCCAGACTC CTCCTATGCA GAGGCCTGTG GAGCCCCAGG 
     2761 AAGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT GCAGCACCTG 
     2821 TACCCCGATC CCAGTCCCTG CAGGACCAGC CGACTCGAAA CCTGGCTGCC TTCCCAGCCT 
     2881 CCCACGACCC TGACCCTGCT GCTGTCCCTA CACCCACTGC CACACCCAGT GCCCGAGGAG 
     2941 CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGGCC AGGGCCTAGC CCAAACCCTC 
     3001 CATCCTGGGT TCGGCCTGAT AATGAGGCTC CACCTAAGGT TCCACAGAGG ACCTCTTCTA 
     3061 TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCTCAG GCTGTCCGTG 
     3121 CCAGACCTCG CAGTAACTCC GCCTGGCAAA TCTATCTGCA GAGGCGGGCA GAGCGGGGCA 
     3181 CCCCCAAGCC TCCCGGGCCC CCAGCTCAGC CCCCTGGCCC GCCCAACGCC TCTAGTAACC 
     3241 CTGACCTCAG GAGGAGTGAC CCTGGCTGGG AGCGCTCAGA CAGTGTCCTC CCGGCCTCCC 
     3301 ACGGCCACCT CCCTCAGGCT GGCTCCTTGG AGCGGAACCG AAACCGTGTG GGAGCCTCCA 
     3361 CAAAACTGGA TAGCTCTCCA GTGCTCTCCC CTGGGAACAA AGCCAAGCCT GAAGACCACC 
     3421 GCTCAAGGCC AGGCCGGCCC GCAAGCTATA AGCGAGCAAT TGGCGAGGAC TTTGTGTTGC 
     3481 TCAAAGAGCG GACTCTGGAT GAGGCCCCTA AGCCTCCCAA GAAGGCCATG GACTACTCCT 
     3541 CATCCAGTGA GGAGGTGGAA AGCAGTGAAG AGGAGGAGGA GGAAGGCGAT GGGGAGCCGT 
     3601 CAGAGGGGAG CAGAGACACT CCCGGGGGCC GTGATGGTGA TACAGACAGC GTCAGCACCA 
     3661 TGGTGGTTCA TGATGTTGAG GAGATATCCG GGACCCAGCC CTCATATGGC GGCGGCACCA 
     3721 TGGTGGTCCA GCGTACTCCT GAAGAGGAAC GAAGCCTGCT GCTTGCTGAT AGCAATGGCT 
     3781 ACACAAACCT GCCTGATGTG GTCCAGCCCA GCCACTCACC TACTGAGAAC AGCAAAGGTC 
     3841 AAAGCCCTCC AACAAAGGAT GGAGGCAGTG ATTACCAGTC TCGTGGGCTG GTAAAGGCCC 
     3901 CAGGAAAGAG CTCATTCACC ATGTTTGTGG ATCTAGGGAT CTACCAGCCT GGAGGCAGTG 
     3961 GGGACACCAT CCCTATCACA GCCCTAGTGG GTGGAGAAGG TGGTCGCCTT GATCAACTGC 
     4021 AGTTCGATGT GAGGAAGGGC TCTGTGGTCA ACGTCAATCC CACCAACACC CGAGCTCATA 
     4081 GTGAAACTCC TGAAATTCGC AAGTACAAGA AGCGATTCAA CTCAGAGATC CTATGTGCAG 
     4141 CTCTCTGGGG GGTCAACCTC CTAGTGGGCA CAGAGAATGG GCTGATGTTG CTGGACCGAA 
     4201 GTGGGCAGGG CAAGGTGTAT GGACTTATTG GGCGACGACG CTTCCAGCAA ATGGATGTCT 
     4261 TAGAAGGGCT CAACTTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTATATT 
     4321 ACCTGTCCTG GCTTCGGAAC AAGATCCTAC ACAATGACCC AGAGGTGGAA AAGAAGCAGG 
     4381 GGTGGACCAC CGTGGGGGAC ATGGAGGGCT GCGGCCACTA CCGTGTTGTG AAATATGAAC 
     4441 GGATTAAGTT CCTGGTCATT GCCCTGAAGA ACTCCGTGGA GGTTTATGCC TGGGCTCCCA 
     4501 AACCCTACCA CAAATTCATG GCCTTCAAGT CCTTTGCTGA CCTCCCTCAC CGCCCTCTAC 
     4561 TGGTGGACCT GACAGTAGAG GAGGGACAGC GGCTCAAGGT CATCTATGGC TCCAGTGCTG 
     4621 GCTTCCATGC TGTGGATGTT GATTCTGGGA ACAGCTATGA CATCTACATC CCTGTACATA 
     4681 TCCAGAGCCA GATCACACCC CACGCCATCA TCTTCCTCCC CAACACTGAT GGCATGGAGA 
     4741 TGCTGCTGTG CTATGAAGAT GAGGGTGTCT ATGTCAACAC TTACGGGCGG ATCATCAAGG 
     4801 ATGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTGGC CTACATCTGC TCCAACCAGA 
     4861 TAATGGGCTG GGGTGAGAAG GCCATAGAGA TCCGCTCTGT GGAGACAGGC CACCTAGATG 
     4921 GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGC AATGACAAGG 
     4981 TGTTTTTTGC CTCTGTCCGC TCTGGAGGAA GCAGCCAAGT TTACTTTATG ACTCTGAACC 
     5041 GTAACTGCAT CATGAACTGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5101 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5161 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5221 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5281 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5341 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5401 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5461 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5521 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5581 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5641 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5701 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5761 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5821 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5881 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5941 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6001 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6061 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6121 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6181 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6241 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6301 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6361 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6421 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6481 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6541 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6601 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6661 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6721 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6781 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6841 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6901 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6961 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7021 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7081 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7141 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7201 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7261 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7321 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7381 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7441 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7501 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7561 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7621 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7681 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7741 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7801 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7861 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7921 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7981 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8041 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8101 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8161 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8221 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8281 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8341 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8401 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8461 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8521 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8581 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8641 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8701 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8761 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8821 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8881 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//