LOCUS       MR224719                8781 bp    DNA     circular  SYN 
DEFINITION  Mink1 (Myc-DDK-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 1
ACCESSION   NM_016713
VERSION     NM_016713.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4938..5029 
                     /label="Myc-DDK"  
     misc_feature    5059..5644 
                     /label="PolyA signal"  
     misc_feature    5116..5135 
                     /label="Restriction Enzyme"  
     misc_feature    5920..6592 
                     /label="ColE1"  
     misc_feature    7022..7816 
                     /label="Kan/Neo"  
     misc_feature    7851..8153 
                     /label="SV40 ori"  
     misc_feature    8239..8716 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4920..4926 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4920 
                     /label="Mink1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACCCA GCCCCCGCCC GCAGCCTGGA CGACATCGAC CTGTCTGCCC 
     1081 TGCGGGACCC TGCAGGAATC TTTGAGCTGG TGGAGGTGGT TGGCAATGGA ACCTATGGAC 
     1141 AGGTATACAA GGGGCGGCAC GTCAAGACTG GGCAGCTGGC TGCCATTAAG GTCATGGATG 
     1201 TCACAGAGGA TGAGGAGGAA GAGATCAAAC AGGAAATCAA CATGTTAAAG AAGTACTCTC 
     1261 ACCATCGCAA TATTGCCACC TACTATGGGG CCTTTATCAA GAAGAGCCCT CCTGGGAACG 
     1321 ATGACCAGCT CTGGCTGGTG ATGGAGTTCT GCGGTGCTGG TTCAGTGACC GACCTGGTAA 
     1381 AGAACACAAA AGGGAACGCA CTGAAGGAGG ATTGCATTGC TTACATCTGC AGGGAGATTC 
     1441 TCAGGGGTCT TGCCCATCTC CATGCCCACA AGGTGATCCA CAGAGATATC AAGGGACAAA 
     1501 ATGTGCTGCT GACAGAGAAT GCTGAAGTCA AGCTAGTGGA TTTTGGGGTG AGTGCTCAGC 
     1561 TGGACCGCAC TGTGGGCAGG CGGAACACTT TCATTGGAAC CCCATACTGG ATGGCTCCAG 
     1621 AAGTCATTGC CTGTGACGAG AACCCCGATG CCACCTATGA CTACAGGAGT GACATTTGGT 
     1681 CTCTAGGAAT CACAGCCATT GAAATGGCAG AGGGAGCCCC CCCTCTGTGT GACATGCACC 
     1741 CTATGCGGGC CCTCTTCCTC ATCCCTCGGA ACCCTCCCCC CAGGCTCAAG TCAAAGAAAT 
     1801 GGTCTAAGAA GTTCACTGAC TTCATCGACA CGTGTCTCAT CAAGACTTAC CTGAGCCGCC 
     1861 CACCCACCGA ACAGTTACTC AAATTCCCCT TCATCCGAGA CCAGCCCACG GAGCGGCAGG 
     1921 TCCGCATCCA GCTCAAGGAC CACATCGACC GCTCGCGGAA GAAGCGGGGT GAGAAAGAGG 
     1981 AGACAGAGTA TGAGTACAGC GGCAGTGAGG AGGAAGACGA CAGCCATGGA GAGGAAGGCG 
     2041 AGCCAAGCTC CATCATGAAT GTGCCCGGTG AGTCCACACT GCGCAGAGAA TTCCTCAGAC 
     2101 TCCAGCAGGA GAATAAGAGC AACTCTGAGG CTTTAAAGCA GCAGCAGCAG CTGCAGCAAC 
     2161 AGCAGCAGCG GGACCCGGAG GCACACATCA AACACCTGCT GCACCAGCGG CAGCGTCGCA 
     2221 TAGAGGAGCA GAAGGAGGAG CGGCGACGTG TGGAGGAGCA ACAGCGGCGA GAGCGAGAAC 
     2281 AGCGTAAGCT ACAAGAGAAG GAGCAGCAGC GGCGATTGGA AGACATGCAA GCCCTACGAC 
     2341 GAGAGGAAGA GAGGCGGCAA GCAGAGCGGG AACAGGAATA CAAGCGGAAG CAGCTGGAGG 
     2401 AGCAGCGGCA GTCAGAGCGG CTGCAGAGAC AGCTGCAGCA GGAGCACGCC TACCTCAAGT 
     2461 CCCTGCAGCA GCAGCAGCAG CAGCAGCAGC TCCAGAAGCA GCAGCAGCAG CAGCAGCAGA 
     2521 TCCTGCCTGG AGACAGGAAG CCCCTGTATC ATTACGGTCG GGGCATTAAT CCTGCTGACA 
     2581 AGCCAGCATG GGCCCGCGAG GTGGAAGAGA GAGCACGGAT GAACAAGCAG CAGAACTCTC 
     2641 CCTTGGCGAA GGCGAAGCCA AGCAGTGCGG GGCCAGAGCC CCCCATCTCC CAGGCCTCTC 
     2701 CTAGCCCCCC AGGACCTCTT TCCCAGACTC CTCCTATGCA GAGGCCTGTG GAGCCCCAGG 
     2761 AAGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT GCAGCACCTG 
     2821 TACCCCGATC CCAGTCCCTG CAGGACCAGC CGACTCGAAA CCTGGCTGCC TTCCCAGCCT 
     2881 CCCACGACCC TGACCCTGCT GCTGTCCCTA CACCCACTGC CACACCCAGT GCCCGAGGAG 
     2941 CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGGCC AGGGCCTAGC CCAAACCCTC 
     3001 CATCCTGGGT TCGGCCTGAT AATGAGGCTC CACCTAAGGT TCCACAGAGG ACCTCTTCTA 
     3061 TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCTCAG GCTGTCCGTG 
     3121 CCAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC AGTGTCCTCC 
     3181 CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA AACCGTGTGG 
     3241 GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA GCCAAGCCTG 
     3301 AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAGACTTTGT GTTGCTCAAA GAGCGGACTC 
     3361 TGGATGAGGC CCCTAAGCCT CCCAAGAAGG CCATGGACTA CTCCTCATCC AGTGAGGAGG 
     3421 TGGAAAGCAG TGAAGAGGAG GAGGAGGAAG GCGATGGGGA GCCGTCAGAG GGGAGCAGAG 
     3481 ACACTCCCGG GGGCCGCAGT GATGGTGATA CAGACAGCGT CAGCACCATG GTGGTTCATG 
     3541 ATGTTGAGGA GATATCCGGG ACCCAGCCCT CATATGGCGG CGGCACCATG GTGGTCCAGC 
     3601 GTACTCCTGA AGAGGAACGA AGCCTGCTGC TTGCTGATAG CAATGGCTAC ACAAACCTGC 
     3661 CTGATGTGGT CCAGCCCAGC CACTCACCTA CTGAGAACAG CAAAGGTCAA AGCCCTCCAA 
     3721 CAAAGGATGG AGGCAGTGAT TACCAGTCTC GTGGGCTGGT AAAGGCCCCA GGAAAGAGCT 
     3781 CATTCACCAT GTTTGTGGAT CTAGGGATCT ACCAGCCTGG AGGCAGTGGG GACACCATCC 
     3841 CTATCACAGC CCTAGTGGGT GGAGAAGGTG GTCGCCTTGA TCAACTGCAG TTCGATGTGA 
     3901 GGAAGGGCTC TGTGGTCAAC GTCAATCCCA CCAACACCCG AGCTCATAGT GAAACTCCTG 
     3961 AAATTCGCAA GTACAAGAAG CGATTCAACT CAGAGATCCT ATGTGCAGCT CTCTGGGGGG 
     4021 TCAACCTCCT AGTGGGCACA GAGAATGGGC TGATGTTGCT GGACCGAAGT GGGCAGGGCA 
     4081 AGGTGTATGG ACTTATTGGG CGACGACGCT TCCAGCAAAT GGATGTCTTA GAAGGGCTCA 
     4141 ACTTGCTCAT CACCATCTCA GGGAAAAGGA ACAAACTGCG GGTATATTAC CTGTCCTGGC 
     4201 TTCGGAACAA GATCCTACAC AATGACCCAG AGGTGGAAAA GAAGCAGGGG TGGACCACCG 
     4261 TGGGGGACAT GGAGGGCTGC GGCCACTACC GTGTTGTGAA ATATGAACGG ATTAAGTTCC 
     4321 TGGTCATTGC CCTGAAGAAC TCCGTGGAGG TTTATGCCTG GGCTCCCAAA CCCTACCACA 
     4381 AATTCATGGC CTTCAAGTCC TTTGCTGACC TCCCTCACCG CCCTCTACTG GTGGACCTGA 
     4441 CAGTAGAGGA GGGACAGCGG CTCAAGGTCA TCTATGGCTC CAGTGCTGGC TTCCATGCTG 
     4501 TGGATGTTGA TTCTGGGAAC AGCTATGACA TCTACATCCC TGTACATATC CAGAGCCAGA 
     4561 TCACACCCCA CGCCATCATC TTCCTCCCCA ACACTGATGG CATGGAGATG CTGCTGTGCT 
     4621 ATGAAGATGA GGGTGTCTAT GTCAACACTT ACGGGCGGAT CATCAAGGAT GTGGTGCTGC 
     4681 AGTGGGGAGA GATGCCCACC TCTGTGGCCT ACATCTGCTC CAACCAGATA ATGGGCTGGG 
     4741 GTGAGAAGGC CATAGAGATC CGCTCTGTGG AGACAGGCCA CCTAGATGGG GTCTTCATGC 
     4801 ACAAACGAGC CCAGAGGCTC AAGTTCCTGT GTGAGCGCAA TGACAAGGTG TTTTTTGCCT 
     4861 CTGTCCGCTC TGGAGGAAGC AGCCAAGTTT ACTTTATGAC TCTGAACCGT AACTGCATCA 
     4921 TGAACTGGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4981 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5041 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5101 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5161 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5221 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5281 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5341 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5401 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5461 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5521 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5581 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5641 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5701 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5761 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5821 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5881 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5941 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6001 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6061 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6121 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6181 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6241 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6301 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6361 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6421 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6481 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6541 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6601 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6661 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6721 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6781 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6841 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6901 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6961 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7021 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7081 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7141 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7201 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7261 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7321 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7381 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7441 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7501 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7561 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7621 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7681 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7741 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7801 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7861 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7921 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7981 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8161 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8221 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8281 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8341 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8401 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8461 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8521 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8581 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8641 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8701 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8761 CAAAAATTTA ACGCGAATTT T
//