LOCUS       MR224646                7782 bp    DNA     circular  SYN 
DEFINITION  Lgr6 (Myc-DDK-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6)
ACCESSION   NM_001033409
VERSION     NM_001033409.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3939..4030 
                     /label="Myc-DDK"  
     misc_feature    4060..4645 
                     /label="PolyA signal"  
     misc_feature    4117..4136 
                     /label="Restriction Enzyme"  
     misc_feature    4921..5593 
                     /label="ColE1"  
     misc_feature    6023..6817 
                     /label="Kan/Neo"  
     misc_feature    6852..7154 
                     /label="SV40 ori"  
     misc_feature    7240..7717 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3921..3927 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3921 
                     /label="Lgr6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACAGCCCG CCTGGGCTCC TGGCGCTGTG GCTTTGCGCT GTGCTGTGCG 
     1081 CATCGGCGCG CGCGGGCAGC GACCCCCAGC CTGGCCCGGG GCGTCCCGCC TGCCCGGCTC 
     1141 CCTGCCACTG CCAGGAGGAC GGCATCATGC TGTCCGCTGA CTGCTCCGAG CTCGGGCTCT 
     1201 CAGTGGTGCC TGCGGACCTG GACCCCCTGA CGGCTTACCT AGACCTCAGT ATGAACAACC 
     1261 TCACGGAGCT TCAGCCGGGT CTCTTCCACC ACCTGCGCTT CCTGGAGGAG CTGCGGCTCT 
     1321 CAGGGAACCA CCTCTCACAC ATCCCGGGAC AGGCATTCTC TGGCCTCCAC AGCCTCAAAA 
     1381 TTCTAATGCT GCAGAGCAAC CAGCTCCGTG GGATCCCAGC AGAGGCACTA TGGGAGCTGC 
     1441 CCAGCCTGCA GTCGCTGCGC CTAGATGCTA ATCTCATCTC CCTGGTCCCT GAGAGAAGCT 
     1501 TTGAGGGGCT CTCCTCCCTC CGCCACCTCT GGCTGGATGA CAATGCACTC ACCGAGATCC 
     1561 CCGTCAGAGC TCTCAACAAC CTTCCTGCCC TACAAGCCAT GACCTTGGCT CTCAACCATA 
     1621 TCCGCCACAT CCCTGACTAT GCCTTCCAGA ACCTCACCAG TCTTGTGGTG CTGCATCTAC 
     1681 ATAACAACCG CATCCAGCAT GTGGGGACCC ACAGCTTCGA GGGGCTGCAC AATCTGGAGA 
     1741 CACTAGACCT GAACTATAAT GAGCTGCAGG AGTTCCCCTT GGCTATCCGG ACCCTGGGCA 
     1801 GGCTGCAGGA ATTGGGTTTC CATAACAACA ACATCAAGGC TATCCCAGAG AAAGCCTTCA 
     1861 TGGGCAGCCC TCTCCTGCAG ACAATACATT TTTATGACAA CCCAATCCAG TTTGTGGGAA 
     1921 GGTCAGCATT CCAGTACCTG TCTAAACTGC ATACGCTATC TTTGAATGGT GCCACTGATA 
     1981 TCCAAGAGTT CCCAGACCTC AAAGGCACCA CTAGCCTGGA GATCCTGACC CTGACCCGTG 
     2041 CGGGCATCAG ACTGCTCCCA CCGGGAGTGT GCCAACAGCT GCCTAGGCTC CGAATCCTGG 
     2101 AGCTGTCTCA TAATCAGATC GAGGAGTTAC CCAGCCTGCA CAGGTGTCAG AAGCTGGAGG 
     2161 AAATTGGCCT CCGACATAAC CGGATCAAGG AAATTGGTGC AGATACCTTC AGCCAGCTGG 
     2221 GCTCCTTGCA AGCTTTAGAC CTGAGTTGGA ATGCCATCCG TGCCATCCAC CCTGAGGCTT 
     2281 TCTCAACCCT TCGATCCTTG GTTAAGCTGG ACCTGACTGA CAACCAGCTG ACCACACTGC 
     2341 CCCTGGCTGG GCTGGGAGGC CTGATGCACC TGAAGCTCAA AGGGAACTTG GCCCTGTCTC 
     2401 AGGCCTTCTC CAAGGACAGT TTCCCAAAAC TGAGGATCCT GGAGGTGCCC TACGCCTACC 
     2461 AGTGCTGTGC CTACGGCATC TGTGCCAGCT TCTTCAAGAC CTCTGGGCAG TGGCAGGCCG 
     2521 AGGACTTTCA TCCAGAGGAA GAGGAGGCAC CAAAGAGGCC CCTGGGTCTC CTTGCTGGAC 
     2581 AAGCTGAGAA CCACTATGAC CTAGACCTGG ATGAGCTCCA GATGGGGACA GAGGACTCAA 
     2641 AGCCACACCC CAGTGTCCAG TGCAGCCCTG TTCCAGGCCC CTTCAAGCCC TGCGAGCACC 
     2701 TCTTTGAGAG CTGGGGCATC CGCCTTGCTG TGTGGGCCAT CGTGTTACTC TCCGTACTCT 
     2761 GTAACGGGCT GGTGCTGCTG ACAGTCTTTG CCAGCGGACC CAGCCCGCTG TCCCCCGTCA 
     2821 AGCTTGTGGT GGGTGCGATG GCAGGCGCCA ACGCCCTGTC GGGCATTTCC TGTGGTCTCC 
     2881 TGGCCTCGGT GGACGCCTTG ACCTATGGTC AGTTCGCTGA GTATGGAGCC CGCTGGGAGA 
     2941 GCGGTCTGGG CTGCCAGGCT ACGGGCTTCC TGGCTGTCCT GGGTTCAGAG GCGTCGGTGC 
     3001 TGCTGCTCAC ACTGGCGGCC GTGCAGTGCA GCATCTCCGT GACCTGCGTC CGAGCCTACG 
     3061 GGAAGGCGCC GTCGCCTGGC AGCGTCCGCG CAGGCGCACT GGGATGCCTG GCGCTGGCCG 
     3121 GGCTGGCCGC AGCACTGCCG CTGGCCTCGG TGGGAGAGTA TGGCGCCTCC CCACTCTGCC 
     3181 TGCCCTACGC CCCACCCGAG GGCCGGCCGG CTGCCCTGGG CTTCGCTGTA GCCCTGGTGA 
     3241 TGATGAACTC GCTCTGCTTC CTGGTGGTGG CCGGCGCCTA CATCAAGCTC TACTGTGACC 
     3301 TGCCACGGGG TGACTTTGAG GCCGTGTGGG ACTGCGCCAT GGTGCGCCAC GTGGCCTGGC 
     3361 TCATCTTTGC AGATGGCCTC CTCTACTGCC CCGTGGCCTT CCTCAGCTTT GCCTCTATGC 
     3421 TGGGCCTCTT CCCTGTCACC CCCGAGGCTG TCAAGTCAGT CCTTCTGGTG GTGCTGCCTC 
     3481 TGCCTGCCTG CCTCAACCCA CTGCTCTACC TGCTCTTCAA CCCTCACTTC CGGGATGACC 
     3541 TTCGGCGGCT CTGGCCAAGC CCTCGGTCCC CAGGGCCCCT AGCCTACGCT GCAGCCGGTG 
     3601 AGCTGGAGAA GAGCTCCTGC GACTCCACCC AAGCGCTGGT GGCTTTCTCA GATGTGGATC 
     3661 TTATTCTGGA AGCTTCTGAG GCTGGGCAGC CTCCTGGGCT AGAGACCTAT GGCTTCCCTT 
     3721 CAGTGACCCT CATCTCCCGA CATCAGCCGG GGGCTACCAG GCTGGAGGGA AACCATTTTG 
     3781 TAGAGTCTGA TGGAACCAAG TTTGGGAACC CACAACCTCC CATGAAGGGA GAACTGCTGC 
     3841 TGAAGGCAGA GGGAGCCACT TTGGCAGGCT GTGGCTCTTC CGTGGGTGGA GCCCTCTGGC 
     3901 CCTCTGGCTC TCTCTTTGCC TCTCACTTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3961 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4021 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4081 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4141 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4201 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4261 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4321 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4381 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4441 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4501 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4561 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4621 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4681 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4741 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4801 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4861 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4921 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4981 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5041 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5101 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5161 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5221 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5281 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5341 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5401 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5461 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5521 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5581 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5641 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5701 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5761 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5821 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5881 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5941 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6001 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6061 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6121 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6181 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6241 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6301 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6361 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6421 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6481 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6541 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6601 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6661 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6721 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6781 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6841 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6901 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6961 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7021 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7081 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7141 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7201 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7261 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7321 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7381 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7441 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7501 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7561 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7621 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7681 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7741 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//