LOCUS       MR224604                8319 bp    DNA     circular  SYN 
DEFINITION  Smg7 (Myc-DDK-tagged) - Mouse Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 1
ACCESSION   NM_001160257
VERSION     NM_001160257.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4476..4567 
                     /label="Myc-DDK"  
     misc_feature    4597..5182 
                     /label="PolyA signal"  
     misc_feature    4654..4673 
                     /label="Restriction Enzyme"  
     misc_feature    5458..6130 
                     /label="ColE1"  
     misc_feature    6560..7354 
                     /label="Kan/Neo"  
     misc_feature    7389..7691 
                     /label="SV40 ori"  
     misc_feature    7777..8254 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4458..4464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4458 
                     /label="Smg7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGACCGAA AACTTGAAAT CAGAGGAGCA TCTGAAATCA AGTAATATTA 
     1081 GGCAGGCAGA AGTCCTGAAG GCTGAAATGA CAGATTCTAA GCTGGGTCCG GCTGAGGTCT 
     1141 GGACATCCAG GCAAGCTCTG CAGGACTTGT ACCAGAAAAT GCTAGTTACT GATCTGGAAT 
     1201 ACGCTTTAGA CAAGAAAGTA GAACAGGATC TCTGGAATCA TGCCTTTAAG AATCAGATCA 
     1261 CAACACTGCA AGGCCAAGCC AAGAATCGAG CAAACCCAAA TCGAAGTGAA GTCCAGGCAA 
     1321 ACCTTTCTCT GTTCCTAGAG GCAGCTAGCG GCTTCTATAC TCAGTTATTA CAGGAACTGT 
     1381 GTACGGTGTT TAATGTAGAT TTGCCATGCC GTGTGAAGTC TTCCCAGTTG GGAATTATTA 
     1441 GCAATAAACA GACGCACAGC AGCACCATAG TGAAGCCACA GTCTAGCTCC TGCTCCTACA 
     1501 TTTGCCAACA CTGCCTCGTC CACCTTGGAG ACATTGCTCG ATACAGAAAT CAGACTAGCC 
     1561 AGGCAGAGTC CTACTATAGG CATGCAGCTC AGCTTGTCCC CTCCAATGGT CAGCCTTACA 
     1621 ATCAGTTGGC TATCTTAGCT TCTTCCAAAG GAGACCACCT GACCACAATT TTCTACTACT 
     1681 GCAGAAGCAT TGCTGTGAAA TTCCCTTTCC CAGCTGCCTC CACTAATCTA CAAAAAGCAC 
     1741 TTTCTAAAGC ACTGGAAAGC CGGGATGAGT TGAAAACCAA GTGGGGTGTT TCTGACTTCA 
     1801 TCAAGGCCTT TATTAAATTC CACGGTCATG TATACCTGAG TAAGAGTTTG GAAAAGCTGA 
     1861 GCCCTCTTCG AGAGAAGTTG GAAGAACAGT TTAAGAGACT GCTGTTCCAA AAGGCTTTCA 
     1921 ACTCTCAACA GTTAGTTCAT GTCACTGTCA TTAACCTGTT TCAACTTCAT CATCTTCGTG 
     1981 ACTTTAGCAA TGAAACAGAG CAACACAGTT ACAGCCAAGA TGAACAGCTG TGTTGGACAC 
     2041 AGTTGCTGGC CCTCTTTATG TCTTTTCTCG GCATCCTGTG CAAATGTCCT CTCCAGAATG 
     2101 ATTCTCAGGA GTCCAACAAT GCCTACCCCC TTCCTGCAGT CAAGGTCTCC ATGGACTGGC 
     2161 TAAGACTCCG ACCTAGAGTC TTTCAAGAAG CAGTGGTGGA TGAAAGACAG TACATTTGGC 
     2221 CCTGGCTAAT TTCTCTTCTA AATAGTTTCC ATCCCCGTGA AGATGATCTC TCAAATACTA 
     2281 ATGCCACACC ACTTCCAGAG GAGTTTGAAT TACAAGGATT CTTGGCTTTA AGACCTTCTT 
     2341 TCAGGAACTT GGATTTTTCC AAAGGCCATC AGGGTATTAC AGGAGACAAA GAGGGTCAAC 
     2401 AACGACGAAT ACGACAGCAG CGTTTGATCT CCATAGGGAA ATGGATTGCT GATAACCAGC 
     2461 CACGGCTGAT TCAGTGTGAA AATGAGGTAG GGAAATTGTT ATTTATCACA GAAATCCCAG 
     2521 AATTAATACT AGAAGACCCC AGTGAAGCCA AAGAGAACCT CATCCTACAA GAAACATCTG 
     2581 TGGTAGAGTC ACTGGCTACG GATGGGAGCC CAGGACTGAA ATCAGTGCTG TCTACAGGCC 
     2641 GAAATCCAAG CAACAGCTGT GACTCAGGAG AGAAACCAGT GGTCACCTTT AAAGAGAACA 
     2701 TTAAGCCACG AGAAGTGAAC CAAGGAAGAA GCTTTCCTCC CAAAGAGGTA AAATCCCAGA 
     2761 CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGGAAAAC GCCTGTCACT CAAACTCCAA 
     2821 GTCAAACGAG TAATTCTCAG TTCATCCCCA TCCATCACCC TGGAGCCTTC CCTCCTCTTC 
     2881 CCAGCCGACC AGGATTCCCG CCCCCAACAT ATGTTATCCC CCCTCCTGTG GCATTTTCTA 
     2941 TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT CTTCAGTCTA 
     3001 CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC ATTCCTTACA 
     3061 GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAG TCACAGCCAC 
     3121 CTTCCCAGCC ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT ACAAGCCAAT 
     3181 TGCAGGTTCA AGCTCTAGCT CAGCAACAGC AATCCCCTAC AAAAGTCATA CCAGCTTTGG 
     3241 GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT GCCTCCAAAC 
     3301 AGCTGTGGAA TCCCCCTCAG GTTCAAAGCC CACTAGGGAA AATTATGCCT GTGAAACAGT 
     3361 CCTACTACCT TCAGACCCAA GACCCTATAA AACTGTTTGA GCCGTCATTG CAACCTCCTG 
     3421 TAATACAGCA GCAGCCTCTA GAGAAAAAAA TGAAGCCTTT CCCCATGGAG CCATATAACC 
     3481 ATAATCCCTC AGAAGTCAAG GTCCCAGAGT TCTACTGGGA TTCTTCCTAC AGCATGGCCG 
     3541 ATAACAGAGC AGTAATGGCT CAGCAACCAA ATATGGACCG CAGGAGCAAA CGGTCACCTG 
     3601 GAGTCTTCCG TCCAGAACAG GATCCTGTGC CCAGGATGCC ATTTGAGGAC CCCAAGAGCT 
     3661 CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA GAGGAAGAGC 
     3721 TGATCTTTTC TAACCCTCCT GATCTTTACC CAGCTCTGCT GGGTCCTCTC GCCTCTCTTC 
     3781 CTGGACGAAG CCTCTTTAAA TCCTTATTGG AGAAGCCTTC TGAGCTCATG TCACATTCAT 
     3841 CTTCTTTCCT GTCCCTCACG GGGTTCTCTG TCAATCAGGA AAGATATCCA AACAGCAGTA 
     3901 TGTTCAATGA AGTGTATGGA AAAAACCTGA CAACCAGCTC CAAGGCAGAA CTGAACCCCT 
     3961 CAGTCGCCTC CCAGGAAACA TCACTGTACT CCCTTTTTGA AGGGACCCCA TGGTCTCCAT 
     4021 CACTTCCTGC CAGTTCAGAT CATTCGACAC CAGCCAGCCA GTCTCCTCAT TCCTCTAACC 
     4081 CAAGCAGCCT GCCCAGTTCT CCTCCAACGC ATAACCATAA TTCTGCTCCA TTCTCTAATT 
     4141 TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGGCGGCC TGCAGATAGG TGGAAAACTG 
     4201 ATAAACCAGC CATGGGTGGT TTTGGTGTTG ATTATCTCTC AGCAACGTCA TCATCTGAGA 
     4261 GCAGTTGGCA TCAGGCTAGC ACTCCGAGTG GCACCTGGAC AGGCCACGGC CCCTCCATGG 
     4321 AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT TCCATGATGC 
     4381 ATCCTGGGCC CTCCGCTCTG GAGCAGTTGT TAATGCAGCA GAAGCAGAAA CAGCAGCGGG 
     4441 GACAAGGTGC CATGAACCCT CCACACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4501 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4561 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4621 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4681 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4741 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4801 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4861 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4921 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4981 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5041 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5101 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5161 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5221 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5281 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5341 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5401 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5461 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5521 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5581 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5641 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5701 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5761 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5821 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5881 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5941 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6001 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6061 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6121 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6241 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6301 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6361 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6421 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6481 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6541 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6601 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6661 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6721 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6781 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6841 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6901 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6961 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7021 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7081 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7141 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7201 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7261 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7321 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7381 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7441 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7501 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7561 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7741 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7801 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7861 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7921 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7981 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8041 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8101 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8161 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8221 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8281 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//