LOCUS       MR224581                9477 bp    DNA     circular  SYN 
DEFINITION  Agl (Myc-DDK-tagged) - Mouse amylo-1,6-glucosidase, 4-alpha-glucanotransferase (Agl)
ACCESSION   NM_001081326
VERSION     NM_001081326.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5634..5725 
                     /label="Myc-DDK"  
     misc_feature    5755..6340 
                     /label="PolyA signal"  
     misc_feature    5812..5831 
                     /label="Restriction Enzyme"  
     misc_feature    6616..7288 
                     /label="ColE1"  
     misc_feature    7718..8512 
                     /label="Kan/Neo"  
     misc_feature    8547..8849 
                     /label="SV40 ori"  
     misc_feature    8935..9412 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5616..5622 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5616 
                     /label="Agl"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACACAGT AAGCAGATTC GAATTTTACT ACTGAACGAG ATGGAGAAGC 
     1081 TAGAGAAGAC CCTCTTCAGA CTTGAACAAG GATTTGAACT ACAGTTCCGA TTAGGCCCAA 
     1141 CTTTACAAGG AAAAGCTGTC ACTGTATATA CAAATTACCC ATTGCCTGGA GAAGCATTTA 
     1201 ATCGAGAAAA GTTCCGTTCC TTGGATTGGG AAAACCCAAC GGAGAGAGAA GACGATTCTG 
     1261 ATAAATACTG TAAACTTCAT CTGCAGCAGT CTGGATCTTT TCAGTACTAC TTCCTTCAAG 
     1321 GAAATGAGAA AAGTGGAGGA GGTTACATAG TCGTGGATCC TATTTTACGC ATTGGAGTTG 
     1381 ATAACCACGT GTTACCCTTG GACTGTGTTA CTCTGCAGAC GTTCTTAGCA AAGTGCTTAG 
     1441 GACCCTTCGA TGAGTGGGAA AGCCGGCTAA GGGTGGCAAA GGAGTCAGGT TACAACATGA 
     1501 TTCACTTCAC CCCACTGCAG ACTCTTGGAC TGTCCCGGTC ATGCTATTCC CTTGCCGATC 
     1561 AGTTAGAGCT AAATCCTGAC TTCTCAAGGC CTAGTAAAAG ATATACCTGG AGTGACGTTG 
     1621 GGCAGCTAGT GGAGAAGCTG AAAAGGGAGT GGAATATTCT TTGTATTACC GATGTGGTCT 
     1681 ACAATCATAC TGCCACTAAT AGTAAGTGGA TCCTTGAACA CCCGGAATCT GCATACAATC 
     1741 TTGTAAATTC TCCACACTTG AAGCCTGCCT GGGTCCTAGA CAGAGCACTC TGGCATTTCT 
     1801 CCTGTGATGT TGCAGATGGG AAGTACAGAG AAAAAGGAGT CCCTGCTTTG ATTGAAAATG 
     1861 ACCAACACAT GAATTGCATT CGAAAAATAA TTTGGGAGGA CATTTTCCCA AGGATTCAAC 
     1921 TCTGGGAGTT TTTCCAAGTA GATGTTCACA AAGCAGTTGA ACAATTTAGA AGACTGCTCT 
     1981 CTCAAGAAAA CAGAAGAGTA ACTAAGTCTG AACCAAAAGA ACATCTTAAA ATTATTCAGG 
     2041 ACCCTGAATA CAGGCGGCGA GGCTGCGCTG TAGATATGGA CACTGCTCTG GCGACTTTTA 
     2101 TACCACATGA CAATGGGCCA GCTGCAATTG AAGAGTGCTG TAACTGGTTC CGTAAGAGAC 
     2161 TGGAGGAGTT AAACTCAGAG AAGCACCACC TCACCAGCTG TCACCAGGAA CAGGCAGTTA 
     2221 ATTGTCTTTT GGGAAACGTG TTTTATGAAC GACTGGCAGG CCATGGCCCT AAACTAGGAC 
     2281 CTGTCACCAG GAAGTATCCT TTAGTTACCA GGTATTTTAC TTTCCCCTTT GGAGAAATGG 
     2341 CCTTATCCGC AGAGGAAGCC CTGATCCACC TCCCAGATAA AGCGTGCTTC CTGATGGCAC 
     2401 ACAACGGGTG GGTGATGGGC GATGACCCGC TCCGGAACTT TGCCGAGCCA GGTTCGGATG 
     2461 TTTATTTAAG GAGAGAACTT ATTTGCTGGG GTGACAGTGT TAAATTGCGC TATGGGAATA 
     2521 AACCCGAGGA CTGCCCTTAT CTGTGGGCAC ACATGAAGAA ATACACCGAG ATAACTGCCA 
     2581 CTCACTTCCA GGGAGTTCGT CTGGACAACT GCCACTCCAC GCCTCTCCAC GTGGCTGAGT 
     2641 ACATGCTGGA TGCTGCTAGG AAATTACAGC CCAATCTTTA TGTAGTGGCT GAGCTGTTCA 
     2701 CAGGAAGCGA AGAGCTGGAC AATATCTTCG TTACTAGACT GGGCATCAGT TCCTTAATAA 
     2761 GAGAGGCAAT GAGCGCGTAT AACAGTCACG AAGAGGGCAG ACTGGTCTAC CGGTACGGAG 
     2821 GGGAACCTGT CGGCTCCTTC GTTCAGCCCT GTTTGAGGCC GCTCATGCCT GCTATCGCGC 
     2881 ATGCCCTGTT TATGGACATC ACCCACGATA ATGAGTGTCC GATTGTGCAC AGATCAGCCT 
     2941 ATGATGCTCT TCCAAGTACC ACAGTTGTTT CCATGGCATG CTGTGCCAGT GGAAGCACAA 
     3001 GAGGCTACGA CGAGCTAGTG CCTCATCAGA TTTCAGTGGT TGCAGAGGAG CGATTTTACA 
     3061 CTAAGTGGAA TCCTGGGGCA TCACCGGCAG ATACAGGGGA TGTTAATGTC CATAGCGGGA 
     3121 TTATTGCAGC CAGATGTGCC ATCAATAGGC TCCATCAAGA GCTTGGAGCC AAGGGTTTCA 
     3181 TTCAAGTGTA TGTGGATCAA GTGGATGAAG ACATAGTGGC TGTAACCAGG CACTCCCCGA 
     3241 GCATCCACCA GTCTGTTGTG GCTGTATCGA GAACTGCTTT TAGGAATCCC AAGACTTCCT 
     3301 TTTACAGCAA AGAAGTGCCT CAGATGTGCA TCCCTGGCAA AATTGAAGAG GTAGTTCTGG 
     3361 AAGCTAGAAC TATTGAAAGG AACACAAAAC CTTATAAGAA AGACGAGAAC TCGATCAATG 
     3421 GAATGCCGAA CATGACAGTG GAACTTAGAG AACACATCCA GCTTCATGAA AGTAAAATTG 
     3481 TCAGACAAGC TGGAGTTGCC ACAAAGGGTC CCAATGAATA TATTCAGGAA ATAGAATTTG 
     3541 AGAACCTATC TCCAGGAAGT GTTATTATAT TCAGAGTTAG TCTCGATCCA CACGCTCAAG 
     3601 TTGCTGTGGG GATTCTTCGG AACCACCTGA CCCAGTTCAG CTCTCACTTT AAGTCCGGAA 
     3661 GCCTTGCTGT TGACAACGCA GATCCTATAT TAAAAATCCC CTTCGCTTCC ATTGCCTCTA 
     3721 AATTGACTTT GGCTGAGCTA AACCAGGTAC TTTACCGATG TGAATCAGAA GAACAAGAAG 
     3781 ACGGTGGCGG TTGCTATGAC ATACCAAACT GGTCATCACT TAAGTACGCA GGACTTCAAG 
     3841 GATTAATGTC CGTGTTGGCA GAAATAAGAC CGAAGAATGA CCTGGGGCAT CCATTCTGTG 
     3901 AAAACTTGAG GTCGGGAGAC TGGATGATCG ACTACGTGAG TGGTCGGCTT ATTTCAAGAT 
     3961 CAGGAAGTAT TGCTGAGGTT GGTAAATGGC TGCAGGCTAT GTTCTTCTAC CTGAAGCAGA 
     4021 TCCCACGCTA CCTCATCCCC TGCTACTTTG ACGCTATATT GATTGGTGCA TACACCACTC 
     4081 TGCTGGATGT AGCGTGGAAA CAGATGTCAA GCTTTGTTCA GAACGGATCC ACCTTCGTGA 
     4141 AGCACCTGTC ACTGGGTTCT GTCCAGATGT GTGGAGTCGG GAAATGCCCT TGTCTGCCGC 
     4201 TGCTTTCTCC CTCGCTCCTG GATGTGCCGT GCAGGTTAAA TGAGATCACA AAGGAGAAGG 
     4261 AGCAGTGCTG TGCCTCCTTA GCTGCAGGCT TACCTCACTT TTCTTCCGGT CTTTTTCGCT 
     4321 GCTGGGGGAG GGACACTTTC ATTGCCCTTC GAGGGATGCT GCTGGTTACT GGACGCTACC 
     4381 TGGAAGCCAG GAACATCATT TTAGCGTTTG CTAGTACCCT GCGGCACGGT CTCATCCCTA 
     4441 ACCTCCTCGG CGAAGGGACA TATGCCAGGT ACAACTGCCG GGACGCCGTG TGGTGGTGGC 
     4501 TGCAGTGCAT TCAGGACTAT TGCAGGACAG TTCCAAACGG CCTGGACATC CTCAAATGCC 
     4561 CGGTGTCCAG GATGTATCCA ACAGATGACT CCGCACCCTT GCCTGCTGGG ACACTGGACC 
     4621 AGCCTTTGTT TGAAGTCATC CAGGAAGCTA TGCAGAGGCA CATGCAGGGC ATCCAGTTCC 
     4681 GAGAGAGGAA CGCCGGTCCA CAGATCGACC GAAACATGAA GGACGAGGGT TTCAACATAA 
     4741 CTGCGGGGAT TGATGAAGAA ACAGGATTTG TTTATGGAGG AAATCGCTTC AACTGTGGCA 
     4801 CGTGGATGGA TAAAATGGGA GAGAGTGACC GAGCTAGGAA CAGAGGGATC CCAGCCACTC 
     4861 CAAGAGATGG GTCTGCTGTG GAAATCGTGG GCTTGTGTAA ATCTGCTGTT CGTTGGTTGC 
     4921 TGGAATTGTC TAAGAAAAAT ATCTTTCCTT ATCATGAAGT CAGAGTAAAA AGACATGGAA 
     4981 AGGTTGTGGC AGTCTCGTAC GATGAGTGGA ACAGAAAAAT ACAAAACAAT TTCGAAAAGC 
     5041 TGTTTCATGT TTCCGAAGAC CCTTCAGATC CTAACGAGAA GCATCCGAAC CTGGTTCACA 
     5101 AACGCGGCAT CTACAAGGAC AGCTATGGTG CGTCGAGTCC TTGGTGTGAC TATCAGCTCA 
     5161 GGCCCAACTT CACCATAGCA ATGGTCGTGG CCCCTGAACT CTTTACTGCT GAGAAGGCTT 
     5221 GGAAGGCTTT GGAAATTGCA GAAAAAAAAT TGCTTGGTCC CCTTGGCATG AAAACTTTAG 
     5281 ACCCAGATGA CATGGTTTAC TGTGGAGTTT ATGATAATGC CTTAGACAAT GACAACTACA 
     5341 ACCTTGCTAG AGGCTTCAAT TACCACCAAG GACCTGAGTG GCTGTGGCCA ATTGGGTATT 
     5401 TTCTTCGTGC AAAGTTGTAT TTTTCCAAAA TGATGGGTCC AGAGACTGCT GCGAAGACTG 
     5461 TATTTTTGGT TAAAAATGTT CTTTCTAGAC ATTATGTTCA TCTTGAGAGA TCCCCATGGA 
     5521 AAGGCCTTCC GGAGCTCACC AATGAGAACG GCCAGTACTG CCCTTTCAGC TGTGAGACAC 
     5581 AGGCGTGGTC AATGGCTGTT GTTCTGGAAA CCCTCTATGA TTTAACGCGT ACGCGGCCGC 
     5641 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5701 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5761 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5821 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5881 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5941 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     6001 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     6061 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     6121 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6181 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6241 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6301 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6361 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6421 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6481 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6541 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6601 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6661 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6721 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6781 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6841 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6901 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6961 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     7021 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     7081 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     7141 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7201 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7261 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7321 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7381 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7441 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7501 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7561 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7621 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7681 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7741 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7801 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7861 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7921 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7981 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     8041 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     8101 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     8161 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8221 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8281 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8341 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8401 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8461 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8521 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8581 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8641 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8701 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8761 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8821 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8881 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8941 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     9001 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     9061 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     9121 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9181 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9241 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9301 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9361 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9421 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//