LOCUS       MR224552                8817 bp    DNA     circular  SYN 
DEFINITION  Rad50 (Myc-DDK-tagged) - Mouse RAD50 homolog (S. cerevisiae) (Rad50)
ACCESSION   NM_009012
VERSION     NM_009012.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4974..5065 
                     /label="Myc-DDK"  
     misc_feature    5095..5680 
                     /label="PolyA signal"  
     misc_feature    5152..5171 
                     /label="Restriction Enzyme"  
     misc_feature    5956..6628 
                     /label="ColE1"  
     misc_feature    7058..7852 
                     /label="Kan/Neo"  
     misc_feature    7887..8189 
                     /label="SV40 ori"  
     misc_feature    8275..8752 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4956..4962 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4956 
                     /label="Rad50"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCGGATC GAAAAGATGA GCATTCTGGG CGTGCGAAGT TTTGGGATAG 
     1081 AGGATAAAGA TAAGCAGATT ATCTCTTTCT TCAGCCCCCT CACAATTTTG GTTGGACCCA 
     1141 ATGGGGCGGG GAAGACGACC ATCATTGAAT GTCTAAAGTA TATTTGTACT GGAGATTTCC 
     1201 CTCCTGGAAC CAAAGGAAAT ACATTTGTTC ATGATCCCAA GGTTGCTCAA GAAACAGACG 
     1261 TGCGGGCCCA GATTCGCCTG CAGTTTCGAG ATGTGAATGG AGAGATGGTA GCTGTGCACA 
     1321 GGTCTATGCT TTGCAGTCAG AAAAATAAAA AAACAGAATT TAAAACCTTG GAAGGAGTCA 
     1381 TTACTAGAAT GAAGCATGGT GAAAAAGTCA GTCTCAGCTC CAAATGTGCA GAAATCGACA 
     1441 GAGAAATGAT AAGTTGTCTT GGGGTTTCCA AGTCTGTGCT AAACAATGTT ATTTTCTGTC 
     1501 ACCAAGAAGA CTCCAACTGG CCTTTAAGTG AAGGAAAGGC TTTGAAGCAA AAATTTGATG 
     1561 AGATTTTTTC GGCAACAAGG TACATTAAAG CCTTAGATAC ACTTCGACAG GTGCGTCAGA 
     1621 CACAGGGTCA GAAAGTAAAA GAGTGTCAAA CGGAATTGAA ATATCTGAAG CAAAATAAGG 
     1681 AGAAAGCGTG TGAGATCCGA GATCAGATTA CGAGTAAGGA AGCCCAGTTA GCGTCTTCGC 
     1741 AGGAAATTGT CAGGTCCTAC GAGGATGAGC TTGAGCCATT GAAGAATCGC CTGAAAGAGA 
     1801 TTGAACACAA CCTGTCTAAA ATAATGAAAC TTGATAATGA AATTAAAGCC TTGGAGAGCC 
     1861 GAAAGAAACA AATGGAGAAA GATAACAGTG AATTAGAACA GAAAATGGAA AAGGTTTTTC 
     1921 AAGGGACTGA TGAGCAGCTA AATGACTTGT ATCACAATCA CCAGAGAACA GTAAGGGAGA 
     1981 AAGAAAGGCG CCTGGTAGAC TGCCAGCGAG AACTGGAGAA GCTGAATAAA GAAGCACGGC 
     2041 TCCTCAACCA GGAAAAAGCA GAGCTGCTTG TGGAGCAGGG TCGTCTACAG CTACAGGCAG 
     2101 ATCGCCATCA AGAGCACATC CGAGCCAGAG ACTCATTAAT TCAGTCTTTG GCAACACATC 
     2161 TTGAATTGGA TGGTTTTGAG CGTGGACCAT TCAGTGAAAG ACAGATTAAA AATTTTCATG 
     2221 AACTTGTGAA AGAGAGACAG GAAAGAGAAG CTAAAACTGC CAGCCAGCTC TTGAGCGACC 
     2281 TGACAGACAA AGAAGCTCTG AAGCAGAGGC AGCTTGATGA GCTGAGGGAC AGGAAGAGCG 
     2341 GACTGGGGAG GACGATTGAG CTGAAGACAG AGATCCTCAC GAAGAAGCAG AGCGAGCTGC 
     2401 GGCACGTGAG GAGTGAGCTG CAGCAGCTGG AGGGCTCCTC CGACAGGATT CTGGAACTGG 
     2461 ACCAGGAGCT CACGAAAGCT GAACGTGAAT TAAGCAAGGC CGAGAAAAAT AGCAGCATAG 
     2521 AAACCCTAAA AGCAGAGGTA ATGAGCCTCC AAAATGAGAA AGCTGACCTG GACAGGAGCT 
     2581 TGCGAAAGCT GGACCAGGAG ATGGAGCAGT TAAACCATCA TACAACAACC CGCACACAGA 
     2641 TGGAGATGCT CACCAAAGAC AAAACTGACA AAGATGAACA GATCAGAAAA ATAAAGTCTA 
     2701 GGCACAGTGA TGAACTAACT TCACTGTTGG GGTACTTTCC TAACAAAAAA CAGCTTGAAG 
     2761 ACTGGCTTCA TTCTAAATCC AAAGAAATTA ATCAGACCAG GGACAGACTT GCCAAACTGA 
     2821 ACAAAGAGCT AGCTTCAGCT GAACAAAATA AAAATCATAT AAATAATGAG CTAAAGAAAA 
     2881 AGGAAGAACA GTTGTCCAGT TATGAAGATA AGCTATTTGA TGTTTGTGGT AGCCAAGATT 
     2941 TGGAAAGTGA TTTGGGCAGA CTTAAAGAAG AAATTGAAAA GTCCTCAAAG CAGCGAGCCA 
     3001 TGCTGGCAGG AGCCACAGCA GTTTACTCCC AGTTCATTAC GCAGCTGACA GATGAAAACC 
     3061 AGTCCTGCTG CCCAGTCTGT CAGAGAGTAT TTCAGACAGA GGCTGAGTTA CAGGAAGTCA 
     3121 TCAGCGACTT GCAGTCCAAG CTGAGGCTTG CTCCAGATAA ACTCAAGTCA ACAGAATCGG 
     3181 AACTGAAGAA AAAAGAGCGG CGCCGTGATG AAATGCTTGG GCTTGTGCCC GTGAGGCAAA 
     3241 GCATAATTGA TTTGAAGGAA AAGGAAATAC CAGAATTAAG AAACCGACTA CAGAGTGTCA 
     3301 ATAGAGACAT ACAGCGCCTA AAGAATGACA TAGAAGAGCA GGAAACACTC TTGGGTACAA 
     3361 TAATGCCTGA AGAAGAAAGT GCTAAAGTGT GCCTGACAGA TGTTACAATC ATGGAGAGGT 
     3421 TCCAGATGGA GCTGAAAGAT GTTGAAAGGA AAATTGCACA GCAGGCAGCT AAGCTGCAGG 
     3481 GAGTAGACTT GGACCGGACT GTCCAGCAGG TTAACCAGGA AAAGCAGGAA AAGCAGCACA 
     3541 GGCTGGATAC AGTTACCAGT AAGATTGAAT TGAACCGTAA GCTTATACAG GACCAGCAGG 
     3601 AACAAATCCA ACACCTGAAA AGTAAAACAA ATGAGCTGAA GTCAGAGAAG CTGCAGATAG 
     3661 CCACCAATTT GCAACGGCGC CAGCAAATGG AGGAGCAGAG TGTGGAATTA TCCACTGAAG 
     3721 TCCAGTCTTT AAACAGAGAA ATAAAGGATG CTAAAGAACA AATAAGCCCT TTGGAGACAG 
     3781 CATTGGAAAA GTTGCAGCAG GAAAAAGAAG AATTAATCCA CAGAAAACAT ACAAGTAACA 
     3841 AAATGGCTCA GGATAAAATT AATGATATCA AAGAGAAAGT TAAAAATATT CATGGTTACA 
     3901 TGAAAGACAT AGAGAATTAT ATTCAAGATG GAAAAGATGA CTACAAGAAG CAAAAAGAAA 
     3961 CTGAACTTAA TGGAGTTGCT GTTCAACTAA ATGAATGTGA GAAACACAGA GAAAAGATAA 
     4021 ATAAAGACAT GGGAACCATG AGGCAAGACA TCGACACACA GAAGATACAG GAAAGGTGGC 
     4081 TACAGGATAA CCTTACACTA AGAAAAAGGA GAGATGAACT AAAAGAAGTG GAAGAAGAAC 
     4141 GAAAACAACA CTTGAAGGAG ATGGGACAAA TGCAAGTTTT ACAAATGAAA AATGAGCATC 
     4201 AGAAGTTGGA AGAGAACATA GACACTATAA AGAGAAATCA CAGTTTGGCA TTGGGGCGTC 
     4261 AGAAAGGCTA TGAAGACGAA ATTCTTCACT TTAAAAAGGA GCTCCGAGAA CCTCAGTTCC 
     4321 GGGATGCCGA GGAAAAGTAC AGAGAAATGA TGATCGTCAT GAGGACCACA GAGCTGGTGA 
     4381 ACAAGGACCT CGACATCTAC TACAAGACTC TTGACCAAGC AATTATGAAA TTTCACAGTA 
     4441 TGAAAATGGA AGAAATCAAC AAAATTATTC GTGATCTTTG GCGGAGTACC TATCGTGGGC 
     4501 AAGATATTGA ATACATAGAA ATTCGATCTG ATGCTGATGA AAATGTATCG GCTTCTGATA 
     4561 AAAGGCGAAA TTACAACTAC CGAGTGGTGA TGCTGAAGGG GGACACAGCC TTGGACATGC 
     4621 GTGGACGATG CAGTGCTGGA CAGAAGGTGC TAGCGTCTCT CATCATCCGA CTGGCCCTGG 
     4681 CGGAAACCTT CTGTCTGAAC TGTGGCATCC TTGCCTTGGA TGAGCCGACA ACAAATCTCG 
     4741 ACCGAGAAAA CATTGAGTCT CTTGCACATG CTTTGGTTGA GATCATAAAA AGTCGCTCAC 
     4801 AGCAGCGCAA CTTCCAACTT CTGGTAATCA CTCACGATGA AGATTTTGTG GAGCTCTTAG 
     4861 GGCGATCTGA GTATGTGGAG AAATTCTACA GGGTGAAGAA GAACATGGAC CAGTGCTCAG 
     4921 AGATTGTCAA GTGCAGCATC AGCTCCCTGG GTTCTTATGT TCACACGCGT ACGCGGCCGC 
     4981 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5041 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5101 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5161 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5221 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5281 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5341 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5401 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5461 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5521 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5581 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5641 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5701 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5761 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5821 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5881 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5941 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6001 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6061 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6121 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6181 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6241 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6301 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6361 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6421 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6481 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6541 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6601 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6661 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6721 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6781 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6841 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6901 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6961 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7021 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7081 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7141 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7201 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7261 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7321 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7381 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7441 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7501 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7561 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7621 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7681 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7741 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7801 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7861 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7921 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7981 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8041 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8221 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8281 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8341 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8401 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8461 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8521 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8581 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8641 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8701 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8761 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//