LOCUS       MR224518                7989 bp    DNA     circular  SYN 
DEFINITION  Itga9 (Myc-DDK-tagged) - Mouse integrin alpha 9 (Itga9), transcript variant 2
ACCESSION   NM_001113514
VERSION     NM_001113514.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4146..4237 
                     /label="Myc-DDK"  
     misc_feature    4267..4852 
                     /label="PolyA signal"  
     misc_feature    4324..4343 
                     /label="Restriction Enzyme"  
     misc_feature    5128..5800 
                     /label="ColE1"  
     misc_feature    6230..7024 
                     /label="Kan/Neo"  
     misc_feature    7059..7361 
                     /label="SV40 ori"  
     misc_feature    7447..7924 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4128..4134 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4128 
                     /label="Itga9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGGCCCG GCTGCGGCGC GGACCGGCGC CGGAGGGCTC CGCGCGCTGC 
     1081 TGCTGGCGCT GGTGGCCGCG GGGGTCCCGG CCGGCGCCTA CAACCTGGAC GCGCAGCGCC 
     1141 CGGTACGCTT CCAGGGCCCC TCAGGCTCCT TCTTCGGCTA CGCGGTGCTG GAGCACTTCC 
     1201 ACGACAACAC GCGCTGGGTC CTCGTGGGTG CACCGAAGGC AGATTCTAAA TATAGCACTT 
     1261 CAGTAAAGTC TCCTGGAGCT GTGTTTAAGT GTCGTGTCCA TACCAACCCT GACCGGAGAT 
     1321 GCACCGAGCT GGACATGGCT CGAGGGAGGA CTCGTGGTGC GCCCTGTGGG AAGACCTGCA 
     1381 GGGGAGACCG GGATGACGAG TGGATGGGGG TGAGCCTGGC CCGGCAGCCC AGAGCAGATG 
     1441 GCCGTGTTTT GGCCTGTGCC CATCGTTGGA AGAACATCTA CTACGAAGCA GACCACATCC 
     1501 TGCCCCATGG ATTCTGCTAC CTCATCCCTT CCAACCTCCA GGCCAAAGGC AAGGTGCTGA 
     1561 TTCCCTGCTA CGAAGAGTAT AAGAAGAAGT ATGGGGAAGA ACATGGCTCC TGCCAGGCCG 
     1621 GAATAGCAGG CTTCTTCACA GAGGAACTGG TGGTCATGGG TGCCCCAGGC TCGTTTTATT 
     1681 GGGCTGGGAC ACTCAAGGTG CTGAACCTCA CGGACAACAC ATATTTTAAG TTGAACGATG 
     1741 AAGCGATTAT GAACAGACGG TATACTTATC TGGGCTATGC AGTGACGGCT GGCCACTTCT 
     1801 CTCATCCATC CATCACTGAT GTGGTAGGGG GTGCCCCACA GGATGAAGGC ATTGGAAAGG 
     1861 TTTATATATT TAGAGCTGAC CGAAGATCAG GGACCTTAGT AAAGATCTTT CAGGCATCAG 
     1921 GAAAAAAGAT GGGCTCTTAC TTCGGCTCCT CCTTGTGTGC AGTCGACCTG AACATGGACG 
     1981 GCCTCTCTGA CTTGCTCGTG GGGGCTCCCA TGTTTTCTGA GATCAGAGAT GAGGGGCAGG 
     2041 TCACCGTCTA CCTCAACCAA GGACATGGAG CCCTCGAGGA ACAGCTGACC CTGACTGGAG 
     2101 ATGCCGCCTA CAACGCGCAC TTTGGGGAGA GCATCGCCAA CCTGGGCGAC ATTGATGATG 
     2161 ACGGGTTCCC AGATGTGGCT GTCGGGGCAC CTAAGGAGGA GGACTTTGCT GGCGCAGTCT 
     2221 ACATCTATCA TGGTGATGCC AATGGGATTG TCCCCAAGTA CTCAATGAAG CTGTCTGGGA 
     2281 GGAGGCTAAA CCCGACCCTG CGGATGTTTG GGCAGTCCAT ATCAGGGGGC ATTGATATGG 
     2341 ATGGAAATGG CTATCCTGAT GTCACCATCG GAGCCTTCCT GTCCGACAGC GTGGTTCTCC 
     2401 TCAGGGCCAG ACCGGTCATC ACGGTGGATG TCTCCATCTT CCTGCCAGGC TCCATCAACA 
     2461 TCACAGCACC TCAGTGTCAC GATGGACAGC AGCCTGTGAA CTGCCTGAAT GTCACCGTGT 
     2521 GCTTCCGGTT CCATGGCAAG AATGTACCAG GAGAAATCGG TCTGAACTAC AATCTGACGG 
     2581 CTGATGTGGC ACAGAAGGAG AAGGGCCAGC TGCCCAGAGT CTATTTTGTG TTGTTTGGAG 
     2641 AGACGGCAGG GCAGGTCTCA GAAAGGCTGC AGCTGTCCCA CATGGACGAA GTGTGTCATC 
     2701 ACTACGTGGC CCACGTCAAG CGGAGAGTCC AGGATGTCAT CAGCCCCATT GTGTTTGAAG 
     2761 CCGCCTACAG CCTGGATGAG CATGTGATGG GTGAGGAAGA CCGGGAGCTG CCAGACCTGA 
     2821 CACCAGTGCT TCGCTGGAAG AAGGGACAAA GGATCTCCCA GAAGAATCAG ACAGTTTTTG 
     2881 AAAGGAATTG CCAATCTGAG GACTGTGCTG CCGACCTGCA GCTTCGGGGG AAACTCCTGC 
     2941 TTTCCAGTGT TGACGAGAAA ACCCCACACC TGGCTTTGGG GGCTGTGAAG AATATCTCTC 
     3001 TAAACATCTC CATCTCCAAC CTTGGAGACG ACGCCTATGA TGCCAACGTG TCCTTTAATG 
     3061 TCTCCAGGGA ACTCTTCTTC ATCAACATGT GGCAGAAGGA GGAGATGGGC ATTTCCTGTG 
     3121 AGCTGCTGGA ATCAGACTTC CTCAAGTGCA GTGTGGGATT TCCTTTCATG AGGTCAAAGT 
     3181 CTAAGTATGA ATTCAGTGTC ATCTTTGATA CAAGCCACCT GTCTGGGGAA GAGGAAATTC 
     3241 TCAGCTTCAT CGTGACTGCT CAGAGTGGCA ACTTGGAGCG CTCTGAAGCC CTGCATGACA 
     3301 ACACTCTCAC ACTGACAGTG CCCCTGGTGC ATGAAGTGGA CACGTCCATC ACTGGAATTG 
     3361 TGTCCCCAAC CTCCTTCGTG TATGGCGAGT CTGTGGACGC ATCCAACTTC ATTCAGCTGG 
     3421 ATGACCAGGA GTGTCATTTC CAACCAGTCA ACATTACTCT CCAGGTCTAC AACATGGGTC 
     3481 CCAGCACCCT TCCTGGGTCA TCTGTCAGCA TCTCCTTCCC CAGCCGGCTG TCACCTGGTG 
     3541 GCGCAGAGAT GTTTCAGGTC CAGGACATGG TGGTGAGCCA AGAGAAGGGT AACTGCTCTC 
     3601 TACAAAGGAA CCCGACCCCC TGCATCATCC CTCAAGAACA AGAGAACATC TTCCACACCA 
     3661 TATTTGCTTT CTTCTCCAAG TCTGGAAGAA AAGTGTTGGA CTGTGAAAAG CCAGGGAGCT 
     3721 TCTGCCTAAC GTTGCACTGC AACCTTAGTG CTCTTCCGAA AGAGGAGAGC CGCACCATCG 
     3781 ACCTATACAT GCTACTGAAC ACAGAGATAC TGAAGAAGGA CAGCTCCTCT GTCATCCAGT 
     3841 TCATGGCTCG AGCCAAGGTG AAGGTGGAGC CTGCCCTGAG AGTGGTGGAG ATAGCTAACG 
     3901 GGAACCCAGA AGAGACTCTG GTGGTCTTCG AGGCCTTGCA CAATCTGGAA CCCCGTGGCT 
     3961 ACGTTGTGGG GTGGATCATC GCCATCAGTT TGCTGGTGGG GATCCTCATT TTTCTGCTGC 
     4021 TGGCTGTGCT CCTGTGGAAG ATGGGCTTCT TCCGCAGAAG GTACAAAGAG ATCATTGAAG 
     4081 CTGAGAAAAA CCGGAAAGAG AATGAAGATG GTTGGGACTG GGTCCAGAAA AACCAGACGC 
     4141 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4201 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4261 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4321 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4381 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4441 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4501 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4561 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4621 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4681 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4741 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4801 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4861 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4921 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4981 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5041 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5101 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5161 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5221 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5281 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5341 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5401 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5461 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5521 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5581 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5641 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5701 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5761 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5821 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5881 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5941 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6001 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6061 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6121 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6181 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6241 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6301 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6361 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6421 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6481 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6541 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6601 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6661 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6721 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6781 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6841 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6901 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6961 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7021 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7081 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7141 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7201 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7321 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7381 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7441 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7501 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7561 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7621 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7681 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7741 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7801 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7861 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7921 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7981 GCGAATTTT
//