LOCUS       MR224460                7581 bp    DNA     circular  SYN 
DEFINITION  Actn3 (Myc-DDK-tagged) - Mouse actinin alpha 3 (Actn3)
ACCESSION   NM_013456
VERSION     NM_013456.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3738..3829 
                     /label="Myc-DDK"  
     misc_feature    3859..4444 
                     /label="PolyA signal"  
     misc_feature    3916..3935 
                     /label="Restriction Enzyme"  
     misc_feature    4720..5392 
                     /label="ColE1"  
     misc_feature    5822..6616 
                     /label="Kan/Neo"  
     misc_feature    6651..6953 
                     /label="SV40 ori"  
     misc_feature    7039..7516 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3720..3726 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3720 
                     /label="Actn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGATGGTT ATGCAGCCCG AGGGTCTGGG GGCCGGGGAG GGGCCCTTCT 
     1081 CTGGCGGTGG GGGCGGCGAG TACATGGAAC AGGAGGAGGA CTGGGACCGC GACTTGCTGT 
     1141 TGGACCCGGC CTGGGAGAAA CAGCAGCGGA AAACCTTCAC TGCCTGGTGC AACTCACATC 
     1201 TGCGCAAGGC AGGCACGCAG ATCGAGAACA TTGAAGAGGA CTTCCGCAAT GGCCTGAAGC 
     1261 TCATGCTGCT CCTGGAGGTC ATTTCCGGAG AGAGGCTGCC TAGGCCCGAC AAAGGCAAGA 
     1321 TGCGCTTCCA CAAAATCGCC AATGTCAATA AAGCCCTAGA CTTCATTGCC AGCAAAGGAG 
     1381 TTAAGCTAGT GTCCATCGGT GCTGAAGAAA TTGTTGACGG GAACCTGAAG ATGACCCTGG 
     1441 GCATGATCTG GACCATCATC CTCCGCTTTG CCATTCAGGA CATCTCTGTA GAAGAGACCT 
     1501 CAGCCAAAGA AGGCTTGCTT CTCTGGTGTC AGCGGAAAAC AGCACCGTAC CGCAATGTCA 
     1561 ACGTACAGAA CTTCCATACC AGCTGGAAGG ATGGCCTGGC CCTCTGTGCT CTCATCCACC 
     1621 GTCACCGGCC AGACCTCATT GACTATGCCA AGCTTCGCAA GGATGACCCA ATTGGAAACC 
     1681 TGAACACTGC CTTCGAGGTG GCAGAGAAAT ACCTGGACAT TCCTAAGATG CTGGATGCAG 
     1741 AAGATATCGT GAACACCCCC AAACCAGATG AGAAAGCCAT CATGACCTAT GTTTCCTGCT 
     1801 TCTACCATGC GTTTGCTGGG GCTGAGCAGG CAGAGACTGC TGCCAACAGG ATCTGCAAGG 
     1861 TCTTAGCTGT GAACCAGGAA AATGAGAAGC TGATGGAGGA GTATGAGAAA CTGGCCAGTG 
     1921 AGCTGTTGGA GTGGATCCGC CGCACTGTCC CATGGCTAGA GAACCGCGTG GGCGAACCCA 
     1981 GCATGAGTGC CATGCAGCGC AAGCTGGAGG ACTTCCGAGA CTATCGACGC CTGCACAAGC 
     2041 CTCCCCGTGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG CAGACCAAGT 
     2101 TGCGCCTGAG CCACCGACCC GCTTTCATGC CCTCCGAGGG CAAGCTGGTT TCGGACATAG 
     2161 CCAATGCGTG GCGGGGACTG GAGCAGGTAG AGAAGGGCTA TGAGGATTGG CTGCTTTCGG 
     2221 AGATCAGGCG TCTGCAGAGG CTTCAGCACC TGGCTGAGAA GTTCCAACAG AAGGCTTCCC 
     2281 TGCATGAAGC TTGGACCCGG GGCAAAGAGG AAATGTTAAA CCAGCACGAC TACGAGTCAG 
     2341 CCTCGCTGCA GGAGGTGCGT GCGCTCTTGC GACGTCATGA GGCCTTTGAG AGCGACCTGG 
     2401 CTGCGCACCA AGACCGGGTG GAACACATCG CGGCCCTGGC CCAGGAACTC AATGAGCTCG 
     2461 ACTACCATGA GGCAGCCTCC GTGAACAGCC GCTGCCAAGC CATCTGTGAC CAGTGGGATA 
     2521 ACTTGGGTAC ACTGACCCAG AAGAGGAGGG ACGCACTAGA GAGGATGGAG AAGCTCCTGG 
     2581 AGACCATTGA CCAGCTGCAG CTGGAGTTTG CTCGGCGGGC AGCCCCCTTC AATAACTGGC 
     2641 TGGATGGGGC TATTGAGGAC CTGCAGGATG TGTGGCTCGT GCACTCTGTG GAGGAGACCC 
     2701 AGAGCCTGCT AACAGCACAC GAACAGTTCA AGGCAACGTT GCCCGAGGCT GATCGAGAGC 
     2761 GAGGCGCCAT CCTGGGCATC CAAGGAGAGA TCCAGAAGAT TTGCCAAACA TACGGACTAC 
     2821 GGCCAAAGTC CGGCAACCCC TACATCACCC TCAGTTCGCA GGACATCAAC AATAAGTGGG 
     2881 ATACAGTCAG AAAGCTGGTA CCCAGCCGTG ACCAGACACT GCAGGAGGAG CTGGCCCGGC 
     2941 AGCAGGTGAA CGAGAGGCTC CGGCGACAGT TTGCAGCCCA GGCTAATGCC ATAGGACCCT 
     3001 GGATCCAGGG AAAAGTGGAG GAAGTAGGAC GGCTGGCAGC TGGGCTGGCC GGCTCTCTGG 
     3061 AGGAGCAGAT GGCAGGTCTG CGGCAGCAGG AACAGAACAT CATCAATTAC AAGAGCAACA 
     3121 TCGACCGGCT GGAGGGTGAC CACCAGCTGC TGCAGGAGAG CCTAGTCTTT GACAACAAGC 
     3181 ACACAGTCTA CAGCATGGAG CACATTCGTG TGGGCTGGGA GCAGTTGCTC ACCTCCATTG 
     3241 CCCGCACCAT CAATGAGGTC GAGAACCAGG TACTGACCCG AGATGCCAAG GGCCTGAGCC 
     3301 AGGAGCAGCT CAACGAATTC CGCGCCTCCT TCAACCACTT TGACCGGAAG CGGAATGGGA 
     3361 TGATGGAGCC CGACGACTTC CGAGCTTGTC TCATCTCCAT GGGTTACGAT CTGGGAGAAG 
     3421 TGGAGTTTGC TCGAATCATG ACCATGGTGG ACCCCAATGC AGCTGGGGTT GTGACCTTCC 
     3481 AAGCCTTCAT TGACTTCATG ACCCGAGAGA CTGCCGAGAC AGACACGGCT GAACAAGTGG 
     3541 TAGCCTCCTT CAAAATCCTG GCAGGAGACA AGAACTACAT TACCCCAGAG GAGCTGCGGA 
     3601 GAGAGCTCCC AGCCGAGCAG GCTGAGTACT GCATCCGTCG GATGGCGCCC TACAAGGGAT 
     3661 CTGGGGCTCC ATCTGGAGCT CTGGACTACG TGGCTTTCTC TAGTGCCCTC TATGGAGAGA 
     3721 GTGACCTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3781 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3841 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3901 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3961 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4021 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4081 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4141 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4201 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4261 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4321 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4381 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4441 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4501 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4561 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4621 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4681 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4741 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4801 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4861 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4921 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4981 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5041 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5101 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5161 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5221 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5281 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5341 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5401 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5461 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5521 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5581 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5641 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5701 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5761 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5821 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5881 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5941 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6001 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6061 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6121 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6181 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6241 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6301 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6361 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6421 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6481 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6541 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6601 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6661 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6721 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6781 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6841 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6901 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6961 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7021 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7081 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7141 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7201 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7261 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7321 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7381 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7441 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7501 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7561 CAAAAATTTA ACGCGAATTT T
//