LOCUS       MR224459                7911 bp    DNA     circular  SYN 
DEFINITION  Phf20 (Myc-DDK-tagged) - Mouse PHD finger protein 20 (Phf20)
ACCESSION   NM_172674
VERSION     NM_172674.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4068..4159 
                     /label="Myc-DDK"  
     misc_feature    4189..4774 
                     /label="PolyA signal"  
     misc_feature    4246..4265 
                     /label="Restriction Enzyme"  
     misc_feature    5050..5722 
                     /label="ColE1"  
     misc_feature    6152..6946 
                     /label="Kan/Neo"  
     misc_feature    6981..7283 
                     /label="SV40 ori"  
     misc_feature    7369..7846 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4050..4056 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4050 
                     /label="Phf20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCAAGCAC CCACCTAACA GACGAGGAAT CAGCTTTGAA GTGGGAGCCC 
     1081 AGTTGGAAGC TCGGGACCGT TTAAAAAACT GGTATCCAGC TCATATTGAA GACATTGACT 
     1141 ACGAAGAAGG GAGAGTTCTC ATCCATTTCA AGCGTTGGAA CCATCGTTAC GATGAGTGGT 
     1201 TTTGCTGGGA CAGTCCTTAT CTGCGCCCTT TAGAGAAGAT CCAGCTGAGG AAAGAGGGTT 
     1261 TACATGATGA GGATGGCTCT TCTGAATTTC AAATAAACCA GCAAGTGCTT GCTTGCTGGT 
     1321 CTGACTGTCG ATTTTATCCA GCCAGAGTCA CTGCTGTGAA CAAGGATGGT ACTTACACTG 
     1381 TGAAATTTTA TGATGGAGTA GTTCAAACTG TCAAACATAT TCATGTCAAA GCTTTTTCCA 
     1441 AAGATCAGAA TATTGTGGGT AATGCTAGGC CTAAAGAAAC AGACCACAAA AGTCTTTCAT 
     1501 CGTCTCCTGA GAAACGAGAG AAGTTTAAAG AACAGAGAAA AGTCACGGTC AATGTGAAGA 
     1561 AAGACAAAGT GGAAAAAGCC TTAAAGACAG AAAAGCGGCC CAAGCAACCT GACAAAGAGG 
     1621 GAAAGCTGAT CTGCTCAGAA AAAGGCAAAG TGTCAGAGAA AAGCCTTCCT AAGAACGAAA 
     1681 AGGAAGATAA GGAGAACATT TCCGAGAACG AGCGGGAGTA CTCCGGGGAT GCCCAGGTGG 
     1741 AAAAGAAGCC TGAGAAGGAC CTTGTGAAGA ACCCACAAGA GAACCTGAAG GAGCCAAAAA 
     1801 GAAAACGAGG CAGACCCCCT TCCATAACTC CTACGGCTGT GGATTCAAAC TCTCAAACTT 
     1861 TGCAACCAAT AACATTGGAA TTGAGAAGAC GGAAAATATC AAAACGAAGT GACACCCCAT 
     1921 TAAAGCGTCC CAGACTCGAC AAAAATTCAC CCCAGGAACA GTCAAAAAAA CGCTCTGAAA 
     1981 ATAGTGACAA AGACTTATCC AGGAGACGGT CCTCCAGGCT GTCCACTAAT GGGACCCGTG 
     2041 AGATCCTAGA TCCTGACTCG ATTGTACCTG ATCTGGTTCA TACGGTTGAT ACAAACCCTC 
     2101 TACCAGACAA GTCACCCAGT GCCAAGGATT CTGCTGAAGG TCAGTTGAAG TCTCCATTGG 
     2161 AAGCTGGCCA GGTCTCTTCT GCATTAACTT GCCACCCCAT TGGGGATGGC CTGGGGGCAG 
     2221 CAGATTTGGA GTTGAATTGC AAGTCAATGG GAGAAAACAC GATGAAAACA GAACCTGTTT 
     2281 CTCCTCTTGC TGAGGTGCAG GAAGTTTCAA CTGTTGAAGT TCCAAATACT TTGAAGAAAG 
     2341 TTGATGACTC TGTGACGTTG AATGTGCCAG CTGTGGACCT AGACCACAAG TTTCGATGCA 
     2401 AGGTTCTGGA CTGTTTGAAA TTTTTCCGCA AGGCTAAATT GCTGCACTAT CATATGAAGT 
     2461 ATTTCCATGG GATGGAGAAG TCACCAGAGC CAGAGGAGGG CCCAGGGAAG ACGCATGTAC 
     2521 AGACTCGGGG CTCTGCGGTG CCTGACAAGA CAAGCCAGGA GAGCCTAACC AGGAAGCGGG 
     2581 TCTCTGCCAG TTCCCCCACT GCAAAAGAGA AGGAAAAGAC TAAAGAGAAG AAATTCAAAG 
     2641 AACTTGTGAG AGTGAAGCCA AAGAAGAAAA AGAAAAAGAA AAAGAAAACC AAGCCTGAAT 
     2701 GTCCCTGCAG TGAGGACATC AGTGATACTT CCCAGGAACC TTCTCCACCC AAAACATTTG 
     2761 CTGTCACCAG GTGTGGGTCC TCACACAAGC CTGGGGTCCA TATGAGCCCG CAGCTCCATG 
     2821 GTTCAGATAA TGGAAACCAC AAAGGGAAAT TGAAAACCTG TGAGGAGGAT AATTTGAGTG 
     2881 AGTCCTCTTC CGAGAGCTTT CTTTGGAGTG ATGAGGAATA TGGTCAAGAT GTTGATGTGA 
     2941 CCACCAACCC AGATGAGGAG CTTGAGGGCG ACGACCGCTA TGATTTTGAG GTGGTCCGCT 
     3001 GCATCTGTGA AGTGCAGGAG GAAAATGACT TCATGATTCA GTGTGAAGAG TGCCAGTGCT 
     3061 GGCAGCACGG GGTCTGCATG GGCTTACTGG AAGAAAACGT GCCTGAGAAA TACACCTGCT 
     3121 ATGTTTGCCA AGACCCTCCA GGTCAGAGGC CTGGCTTCAA GTACTGGTAT GACAAGGAGT 
     3181 GGCTGAGCCG GGGACACATG CATGGTCTGG CATTTCTGGA TCAGAACTAC TCCCACCAGA 
     3241 ATGCCAGGAA GATCGTGGCC ACCCACCAGC TGCTCGGGGA CGTGCAGAGA GTGATCCAAG 
     3301 TGCTGCACGG CCTGCAGCTC AAGATGAGCA TTCTGCAAAG CAGAGAGCAT CCTGATCTGC 
     3361 AGCTATGGTG CCAGCCCTGG AAACAGCACT CGGGGGAGGG AAGAGCACAT CCCAGACACA 
     3421 TCCACATCAC AGACGCCAGG AGCGAGGAGT CCCCAAGCTA TAGAACTTTG AATGGGGCGG 
     3481 TGGAGAAGCC ATCGCCCCTG CCCCGATCTG TGGAGGAGTC TTACATCACC AGTGAGCATT 
     3541 GCTACCAGAA ACCTCGCGCC TATTATCCCG CTGTGGAGCA GAGGCTGGTC GTGGAGACGA 
     3601 GAGGCTCTGC CCTTGATGCT GCAGTCAGCC CCCTCTGTGA GAACGGCGAC GACTCCCTCT 
     3661 CCCCTCGCCT AGGCTGGCCC ATCGACCAAG ACAGGAGCAG AGGCGACATA GATCCCAAAC 
     3721 CCAGCTCCCC AAAGGTGAGA GAGTACATTT CCAAGAATGT CTTGCCAGAA GAGACGCCTG 
     3781 CAAGGAAGCT GCTGGACAGA GGTGGAGAAG GGCTGGTGAG CTCTCAGCAC CAGTGGCAGT 
     3841 TCAACCTGCT CACACATGTG GAGTCCCTGC AGGATGAGGT GACGCACCGG ATGGACTCCA 
     3901 TTGAGAAGGA GCTGGACGTG CTGGAGAGCT GGCTGGACTA CACTGGGGAG CTGGAGCCCC 
     3961 CAGAGCCACT GGCCAGGCTT CCGCAGCTCA AGCACTGCAT CAAGCAGCTG CTGACTGACC 
     4021 TGGGCAAGGT GCAGCAGATC GCCCTCTGCT GCTCGACAAC GCGTACGCGG CCGCTCGAGC 
     4081 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4141 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4201 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4261 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4321 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4381 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4441 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4501 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4561 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4621 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4681 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4741 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4801 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4861 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4921 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4981 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5041 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5101 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5161 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5221 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5281 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5341 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5401 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5461 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5521 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5581 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5641 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5701 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5761 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5821 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5881 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5941 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6001 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6061 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6121 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6181 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6241 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6301 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6361 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6421 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6481 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6541 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6601 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6661 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6721 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6781 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6841 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6901 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6961 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7021 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7081 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7141 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7201 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7261 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7321 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7381 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7441 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7501 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7561 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7621 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7681 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7741 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7801 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7861 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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