LOCUS       MR224446                8289 bp    DNA     circular  SYN 
DEFINITION  Itga7 (Myc-DDK-tagged) - Mouse integrin alpha 7 (Itga7)
ACCESSION   NM_008398
VERSION     NM_008398.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4446..4537 
                     /label="Myc-DDK"  
     misc_feature    4567..5152 
                     /label="PolyA signal"  
     misc_feature    4624..4643 
                     /label="Restriction Enzyme"  
     misc_feature    5428..6100 
                     /label="ColE1"  
     misc_feature    6530..7324 
                     /label="Kan/Neo"  
     misc_feature    7359..7661 
                     /label="SV40 ori"  
     misc_feature    7747..8224 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4428..4434 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4428 
                     /label="Itga7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGGATT CCGAGGTGCG ATTTTCTCCG GCCCCCCGGA ATCTACTATC 
     1081 TTATTACTTC CTTGCTCGCT GGACTGTTCT TACCACCGGC TATCGCCTTC AATCTGGATG 
     1141 TGATGGGTGC CATACGCAAG GAGGGAGAAC CCGGCAGCCT ATTCGGCTTT TCGGTGGCCC 
     1201 TGCACCGACA ATTACAGCCC CGACCCCAGA GCTGGCTGCT GGTGGGCGCT CCCCAGGCCC 
     1261 TGGCTCTTCC TGGACAGCAG GCAAACCGCA CCGGAGGCCT CTTTGCTTGT CCCCTGAGCC 
     1321 TAGAGGAGAC AGATTGCTAC AGAGTGGACA TTGACAGGGG AGCTAACGTG CAGAAGGAGA 
     1381 GCAAGGAGAA CCAATGGCTG GGAGTCAGTG TCCGGAGCCA GGGGCCAGGG GGCAAGATTG 
     1441 TTACGTGTGC ACACCGATAT GAGTCTCGAC AGAGAGTGGA CCAGGCTTTG GAGACTCGGG 
     1501 ATGTGATTGG CCGCTGCTTT GTGCTAAGCC AGGACCTGGC CATCCGTGAT GAGCTGGATG 
     1561 GTGGGGAGTG GAAGTTCTGT GAGGGGCGCC CCCAGGGCCA TGAACAGTTT GGGTTCTGTC 
     1621 AGCAGGGCAC AGCTGCCACC TTCTCCCCTG ACAGTCACTA CCTCGTCTTT GGGGCTCCAG 
     1681 GAACCTATAA CTGGAAGGGG TTGCTTTTTG TGACCAACAT TGATAGCTCA GACCCTGACC 
     1741 AGCTGGTGTA TAAAACTTTG GACCCTGCTG ACCGGCTCAC AGGACCAGCC GGAGACTTGA 
     1801 CCTTGAATAG CTATTTAGGT TTCTCCATCG ATTCTGGGAA GGGTCTTATG CGCTCAGAAG 
     1861 AGCTGAGTTT TGTGGCAGGG GCCCCACGTG CCAACCACAA AGGGGCTGTG GTCATTCTGC 
     1921 GCAAGGATAG CGCCAGCCGC TTGATACCTG AGGTTGTGCT GTCTGGGGAG CGCCTGACCT 
     1981 CTGGCTTTGG CTACTCACTG GCTGTGACTG ATCTCAACAA TGATGGCTGG GCAGACCTGA 
     2041 TTGTGGGTGC CCCCTACTTC TTTGAACGCC AAGAAGAGCT GGGAGGTGCT GTGTATGTGT 
     2101 ACATGAACCA GGGTGGCCAT TGGGCAGATA TCTCTCCTCT CCGAATCTGT GGCTCCCCTG 
     2161 ATTCCATGTT TGGGATCAGT TTGGCTGTAT TGGGGGACCT CAACCAAGAT GGCTTCCCAG 
     2221 ACATTGCCGT GGGAGCTCCC TTTGACGGAG ATGGGAAGGT CTTTATCTAC CATGGGAGCA 
     2281 GCCTTGGGGT GGTTGTCAAA CCTTCACAGG TGCTGGAGGG CGAGGCGGTG GGTATCAAGA 
     2341 GCTTTGGTTA CTCCCTGTCT GGTGGCCTGG ATGTGGATGG AAATCACTAC CCAGACCTGC 
     2401 TTGTGGGCTC CCTGGCTGAT ACCGCTGCTC TGTTCAGGGC CAGACCTGTT CTACATGTCT 
     2461 CCCAAGAGAT CTTCATTGAC CCCAGAGCCA TCGATCTGGA ACAACCCAAC TGTGCCGATG 
     2521 GACGCTTGGT CTGTGTGGAC ATAAAGATCT GTTTCAGCTA TGTCGCTGTG CCCAGCAGCT 
     2581 ACAGCCCTAG TGTGGCCTTA GATTATATGT TAGATGGGGA CACAGACCGG AGGCTCCGGG 
     2641 GCCAGGTTCC ACGTGTGACG TTTCTGAGCC GAGGCCTGGA TGACCTCAGG CATCAGTCCT 
     2701 CCGGCACCGT GTGGCTGAAG CACCAACATG ACCGAGTCTG TGGAGATACT GTGTTCCAGC 
     2761 TGCAGGAAAA CGTCAAAGAC AAGCTACGGG CCATTGTGGT GACCCTGTCA TATGGTCTCC 
     2821 GAACCCCTCC ATTAGGGAGG CAAGCACCTG GCCAGGAGCT CCCCACTGTG GCTCCCATCC 
     2881 TCAATGCTCA CCAGCCCAGC ACCCAGAGGA CTGAGATCCA CTTTCTGAAG CAAGGCTGCG 
     2941 GTGAAGATAA GATCTGTCAG AGCAACCTCC AGCTCGAGCG GTACCAGTTC TGTTCCAGGA 
     3001 TCAGCGACAC AGAGTTCCAG GCTCTGCCCA TGGATCTGGA TGGAAGGACC GCCCTGTTCG 
     3061 CATTGAGTGG GCAGCCGTTC ATAGGCCTGG AACTGACAGT CACCAACCTG CCCTCTGACC 
     3121 CTGCCCGGCC TCAGGCAGAT GGGGATGATG CTCATGAAGC ACAGCTCTTG GTCACTCTCC 
     3181 CAGCCTCTCT ACGGTACTCA GGAGTCCGTG CTCTGGACTC TGTGGAGAAG CCGCTCTGCC 
     3241 TGTCCAATGA CAGCGCCTCT CATGTCGAGT GTGAGCTGGG GAACCCTATG AAGAGAGGCG 
     3301 CTCAGGTCAC TTTTTACCTC ATCCTCAGCA CCTCTGGGAT CACTATTGAG ACCACAGAGC 
     3361 TGGAGGTGAA ATTGCTGTTA GCCACGATCA GTGAGCAGGA GCTGGATCCG GTCTCCGTTC 
     3421 GGGCTCATGT CTTCATTGAA TTGCCACTGT CCATTTCAGG GGTGGCCACT CCCCAGCAAC 
     3481 TCTTCTTCTC TGGCGAGGTG AAGGGCGAAA GTGCCATGCG ATCTGAGAGG GACGTGGGCA 
     3541 GCAAAGTCAA GTATGAGGTC ACGGTCTCCA ATCAAGGCCA GTCTCTCAAT ACTCTGGGCT 
     3601 CTGCGTTCCT CAACATCATG TGGCCCCACG AGATCGCCAA TGGAAAGTGG CTGCTGTACC 
     3661 CCATGCGGGT AGAGCTGGAG GGCGGACAGG GGCCTGGCAA GAGAGGGATC TGTTCCCCAA 
     3721 GACCCAACAT CCTCCAGCTG GATGTGGACA GCAGGGATAG GAGGCGGCGA GAGCTGGGGC 
     3781 AGCCGGAGCC GCAGGAGCCT CCAGAGAAGG TGGAGCCTAG CACATCCTGG TGGCCAGTGT 
     3841 CCTCTGCTGA GAAGAGAAAC GTGACTCTGG ACTGCGCCCA GGGCACGGCC AAGTGTGTGG 
     3901 TGTTCAGCTG CCCACTCTAC AGCTTTGATC GTGCAGCCGT GCTTCACGTC TGGGGCCGCC 
     3961 TCTGGAACAG CACCTTTCTG GAGGAGTACA TGGCCGTGAA ATCCCTGGAA GTGATCGTCC 
     4021 GAGCCAACAT CACAGTGAAA TCCTCCATTA AGAACTTGTT GCTCAGAGAT GCATCCACAG 
     4081 TGATTCCAGT GATGGTGTAC TTGGACCCCA TGGCCGTGGT TGTGGAAGGA GTCCCCTGGT 
     4141 GGGTCATCCT CCTGGCAGTG CTGGCTGGGC TGTTAGTCCT GGCCTTGCTG GTACTGCTGC 
     4201 TGTGGAAGCT GGGATTCTTC AAGCGGGCGA AGCACCCCGA GGCCACCGTG CCCCAGTACC 
     4261 ACGCAGTGAA GATCCCTCGG GAAGACCGAC AGCAGTTCAA GGAGGAGAAG ACAGGCACCA 
     4321 TCCAGAGGAG TAACTGGGGC AACTCCCAGT GGGAGGGCTC CGACGCACAC CCCATCTTGG 
     4381 CTGCCGACTG GCATCCTGAG CTGGGTCCTG ATGGACATCC GGTGCCAGCC ACTGCCACGC 
     4441 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4501 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4561 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4621 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4681 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4741 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4801 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4861 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4921 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4981 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5041 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5101 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5161 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5221 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5281 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5341 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5401 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5461 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5521 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5581 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5641 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5701 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5761 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5821 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5881 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5941 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6001 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6061 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6121 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6181 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6241 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6301 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6361 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6421 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6481 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6541 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6601 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6661 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6721 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6781 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6841 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6901 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6961 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7021 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7081 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7141 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7201 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7261 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7321 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7381 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7441 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7501 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7741 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7801 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7861 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7921 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7981 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8041 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8101 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8161 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8221 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8281 GCGAATTTT
//