LOCUS       MR224434                8277 bp    DNA     circular  SYN 
DEFINITION  Inpp5f (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase F (Inpp5f)
ACCESSION   NM_178641
VERSION     NM_178641.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4434..4525 
                     /label="Myc-DDK"  
     misc_feature    4555..5140 
                     /label="PolyA signal"  
     misc_feature    4612..4631 
                     /label="Restriction Enzyme"  
     misc_feature    5416..6088 
                     /label="ColE1"  
     misc_feature    6518..7312 
                     /label="Kan/Neo"  
     misc_feature    7347..7649 
                     /label="SV40 ori"  
     misc_feature    7735..8212 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4416..4422 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4416 
                     /label="Inpp5f"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCTTT CAGGCTAAGG ACCACTACAT CCTGCAGCAG GGCGAGCGTG 
     1081 CCCTGTGGTG CAGCCGGCGC GACGGCGGCC TGCAGCTCCG ACCGGCCACG GATCTGCTTC 
     1141 TTGCCTGGAA CCCCATTTGT TTGGGATTAG TGGAAGGTGT AATTGGGAAA ATTCAACTTC 
     1201 ATTCAGATCT TCCATGGTGG CTTATTTTAA TTCGGCAGAA AGCGTTGGTA GGCAAACTCC 
     1261 CAGGAGACCA TGAGGTCTGT AAAGTCACCA AGATTGCTGT ATTGTCACTT TCTGAAATGG 
     1321 AACCTCAGGA GCTTGAGCTG GAGCTCTGTA AGAAGCATCA TTTTGGAATT AACAAGCCTG 
     1381 AGAAGATTAT ACCATCTCCT GACGACTCCA AGTTTCTACT GAAGACATTT ACAAATATCA 
     1441 AATCCAACGT GTCTGCTCCC AACAAAAAGA AAGTTAAAGA AAGTAAAGAA AAGGAGAAAC 
     1501 TGGAGCGGCG TTTGCTTGAG GAGTTGCTGA AGATGTTCAT GGACTCAGAG TCCTTCTATT 
     1561 ATAGCTTGAC CTATGACCTG ACCAACTCGG TGCAGAGACA GAGCACTGGG GAGAGGGATG 
     1621 GCCGTCCCCT GTGGCAGAAG GTTGATGACC GATTTTTCTG GAATAAGTAC ATGATCCAAG 
     1681 CTCTCACTGA GATTGGTACT CCGGATGTGG ACTTCTGGAT CATCCCCATT ATCCAGGGTT 
     1741 TTGTGCAGAT TGAAGAACTT GTGGTTAATT ACAATGAGTC CTCGGATGAT GACAAGAGCA 
     1801 GCCCAGAGAC GCCCCCTCAG GACTCCACTT GTGTAGATGA CATTCACCCA CGATTTCTAG 
     1861 TGGCTCTTAT ATCTCGAAGA AGTAGGCACA GAGCAGGAAT GCGGTATAAA CGAAGAGGAG 
     1921 TGGATAAAAA TGGAAATGTT GCGAATTATG TGGAGACTGA GCAGTTAATC CATGTTCATC 
     1981 ATCATACCCT GTCATTTATC CAAACTCGAG GCTCTGTGCC GGTCTTTTGG AGCCAGGTTG 
     2041 GGTATCGCTA TAATCCAAGA CCTCGGCTAG ACAAAAGTGA GAAGGAAACG GTTGACTGTT 
     2101 TCTGTGCCCA TTTTGAAGAA CAACTGAAAA TTTACAAAAA ACAGGTTATT GTTAACTTGG 
     2161 TAGACCAGGC TGGAAGGGAG AAGATTATTG GTGATGCTTA CCTGAAGCAG GTGCTGCTCT 
     2221 TCAACAACCC CAAGCTCACC TATGTCTCCT TCGATTTCCA TGAGCACTGC CGAGGAATGA 
     2281 AGTTCGAGAA TGTTCAAACC CTCACGGATG CCATTCATGA CATCATCATT GACATGAAGT 
     2341 GGTGTTGGGT TGATCAAGCT GGTGTGATAT GTAAGCAAGA AGGGATTTTT CGAGTTAATT 
     2401 GCATGGACTG CCTGGATCGC ACCAACGTGG TCCAGGCTGC CATAGCGCGC GTGGTCATGG 
     2461 AACAGCAGCT TAAAAAACTA GGTGTGATGC CCCCAGAGCA GCCACTACCT GTGAAATGTA 
     2521 ATCGGACCTA CCAGATAATG TGGGCCAACA ACGGTGACTC CATCAGCAGG CAGTATGCTG 
     2581 GGACTGCTGC TCTAAAGGGT GACTTTACAA GAACAGGAGA AAGGAAGTTG GCAGGAGTTA 
     2641 TGAAAGATGG CGTTAACTCG GCAAATAGGT ACTACCTCAG TCGGTTCAAG GATGCCTACA 
     2701 GGCAAGCCGT CATAGATCTG ATGCAAGGCG TCCCAGTGAC AGAAGACCTT TACTCCATAT 
     2761 TTACCAAGGA GAAGGAGCAT GAAGCTTTGC ATAAGGAGAG CCAGAGAAGC CACCAGGAGC 
     2821 TCATCAGCCA GCTCTTACAG AGTTATATGC AGTTGCTGCT GCCGGGTGAC GAGAAGTTCC 
     2881 ACGGGGGCTG GGCCCTGGTT GACTGCGACC CCAGCCTCAC TGACGCTGCG CACAGAGATG 
     2941 TGGAAGTGCT GCTGCTGCTG TCTAACGCTG CCTACTATGT GGCCTATTAT GATGACGAAG 
     3001 TTGATAAAGT AAACCAGTAT CAACGACTGG GCCTAGAAGA CCTGGAAAGA ATAGAAATAG 
     3061 GTCCTGAACC TACTCTTTTC GGCAAACCCA AGTTCTCGTG CATGCGGCTG CACTACAGGT 
     3121 GTAAAGAAGC AGGCGGTTAC TTCCATACGC TAAGAGCTGT GCCACGTAGT CCTGAAGAAG 
     3181 ATGGCAAAGA CACTCTTCAG TGCATCGCAG AGATGCTGCA GATCACCAAG CAAGCCATGG 
     3241 GACTGGACGT TCCTATCATT GAGAAGAAAC TTGAGAGGAA AAGCAGTAAG CCTCATGAAG 
     3301 ACATCATTGG TATCAGATCT CAAAACCAAG GCTCTCTTGC CCAGGGGAAA AGCTTTTTAA 
     3361 TGAGCAAATT TTCATCTCTA AATCAAAAAG TAAAGCAGAC CAAATCCAAT GTGAACATCG 
     3421 GCAACCTACG AAAATTAGGA AACTTTACAA AGCCTGAAAT GAAAGTTAAC TTTCTAAAAC 
     3481 CAAACTTAAA AGTCAATCTT TGGAAGTCAG ATAGTAGTCT TGAGACCATG GAAAACCCAG 
     3541 GAGTGATGGG TAATAAGGTC CAGGGGGAGT CTGATGGGGA CATTTCTTCA GATAACGACT 
     3601 CGTACCACTC TGATGAATTC CTTACAAATT CTAAGTCGGA GGAAGACAAG CAGCTAGCCA 
     3661 ATTCTTTAGA GAGCGTAGGG CCAATAGATT ACATTCTCCC AAGCTGTGGG ATTATTGTGT 
     3721 CAGCACCTCG ATTAGGCAGT CGGTCTCAGT CTGCCAGCAG TATCGACGTC AGCACTCATG 
     3781 CCCCCTCAGA AGCTGCTGCT GGTCCTGGAA GTGAACTTGG GAAAGGCCTG GAGTCTCCCC 
     3841 TGAAGAAAAG CCCTTCTGCT GACAGCATAC ACACACGGAC TGGCTTCACA AAGCCCATGG 
     3901 ATGTTTACTG CCAGAGATTT GTGCAGGATG CACAGAACAA AATGAATGAC CTGTCAGAGA 
     3961 TCAGGTCTGT AGCTCAGAAG AGTGAGGAGG GAAGTCACAA GACTAACCGT GTGTCTAATG 
     4021 AAGAAACCCA GTCAGAACCA ATGGGACAGA CGCCTCCTCG ACCCTCTCAG TTAAACGTCT 
     4081 CTTGTTCTGT GGCAGGCCCA CCATTCTTAT CAGTTGAACC AGTACATTCA GTGCTATCTC 
     4141 AGAAGACGCC CAGCTCCGGG TCCAGCCTTC TGGAACTTGA GGCAGGGCTC TGCGTAACTC 
     4201 CCTCTTCAGA GAGCAGCAGC AGCAGGGCAG TCTCTCCCTT TGCAAAGATC CGCAGCTCCA 
     4261 TGGTCCAAGT CGCTAATATT ACCCAAGCTG GGCTAACTCA TGGGATAAAC TTGGCGGTTG 
     4321 CCAAAGTTCA GAAGAGTCCA GCAGAACCCG AAGCGGTTAA CGAAATTCAA CAAAATGAAC 
     4381 TTAAAAATAT GTTTACACAA TGCCAGACAC GAATAATTCA GATTACGCGT ACGCGGCCGC 
     4441 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4501 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4561 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4621 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4681 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4741 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4801 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4861 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4921 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4981 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5041 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5101 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5161 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5221 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5281 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5341 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5401 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5461 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5521 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5581 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5641 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5701 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5761 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5821 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5881 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5941 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6001 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6061 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6121 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6181 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6241 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6301 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6361 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6421 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6481 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6541 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6601 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6661 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6721 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6781 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6841 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6901 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6961 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7021 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7081 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7141 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7201 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7261 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7321 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7381 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7441 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7501 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7681 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7741 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7801 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7861 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7921 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7981 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8041 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8101 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8161 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8221 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//