LOCUS       MR224389                8487 bp    DNA     circular  SYN 
DEFINITION  Slk (Myc-DDK-tagged) - Mouse STE20-like kinase (yeast) (Slk), transcript variant 2
ACCESSION   NM_001164639
VERSION     NM_001164639.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4644..4735 
                     /label="Myc-DDK"  
     misc_feature    4765..5350 
                     /label="PolyA signal"  
     misc_feature    4822..4841 
                     /label="Restriction Enzyme"  
     misc_feature    5626..6298 
                     /label="ColE1"  
     misc_feature    6728..7522 
                     /label="Kan/Neo"  
     misc_feature    7557..7859 
                     /label="SV40 ori"  
     misc_feature    7945..8422 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4626..4632 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4626 
                     /label="Slk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTTCTTC AATTTCCGTA AGATCTTCAA GTTGGGGAGC GAGAAGAAGA 
     1081 AGAAACAGTA CGAACACGTG AAGAGAGACC TGAACCCCGA AGAGTTTTGG GAGATTATTG 
     1141 GAGAACTGGG CGACGGAGCC TTCGGGAAAG TCTATAAGGC CCAGAATAAA GAGACCAATG 
     1201 TTTTAGCTGC TGCAAAGGTG ATTGACACCA AATCTGAAGA AGAGCTTGAA GATTATATGG 
     1261 TTGAGATTGA CATATTAGCA TCTTGTGATC ACCCAAACAT CGTCAAGCTT CTAGATGCCT 
     1321 TCTATTACGA GAACAACCTT TGGATCCTCA TTGAATTCTG TGCAGGGGGA GCAGTGGATG 
     1381 CTGTGATGCT TGAACTTGAG AGACCATTAA CTGAATCCCA AATCCAAGTA GTCTGCAAGC 
     1441 AGACATTAGA GGCATTGAAT TACTTACATG ACAATAAAAT CATCCACCGA GATCTAAAAG 
     1501 CTGGCAATAT TCTCTTTACC TTAGATGGAG ACATTAAATT AGCGGATTTT GGAGTATCAG 
     1561 CTAAAAATAC CAGGACAATT CAAAGGAGGG ATTCATTTAT TGGCACACCA TATTGGATGG 
     1621 CTCCTGAAGT AGTCATGTGT GAGACATCAA AGGACAGACC TTATGACTAC AAAGCTGATG 
     1681 TTTGGTCCCT GGGTATTACT TTAATAGAAA TGGCTGAGAT AGAGCCACCT CATCATGAGT 
     1741 TAAATCCAAT GCGCGTGCTG CTGAAAATTG CAAAATCTGA GCCCCCAACA TTAGCACAGC 
     1801 CATCAAAATG GTCTTCAAAT TTTAAGGACT TTCTAAGGAA ATGCTTGGAA AAGAATGTGG 
     1861 ATGCGCGGTG GACCACGTCT CAGCTGTTGC AGCATCCCTT TGTTACCGTT GATTCCAACA 
     1921 AACCAGTCCG AGAGTTGATT GCTGAGGCAA AGGCTGAAGT AACAGAAGAA GTTGAAGATG 
     1981 GCAAGGAAGA AGATGAGGAG GAGGAAGCAG AGAATGCTCT GCCAATACCT GCAAATAAAC 
     2041 GTGCCTCCTC TGACCTCAGC ATTGCCAGCT CTGAAGAAGA TAAACTTTCA CAAAATGCTT 
     2101 GTATTTTGGA ATCTGTGTCA GAAAGAACAG AACAAAGTAC TTCTGAGGAT AAATTTAGCA 
     2161 ACAAAATTCT TAATGAGAAA CCTACGACTG ACGGTCCTGA GAAGGCTGTG GATGAGCATG 
     2221 CAAGTGATGT GAACTTAGAA ACTGGGGCTG AACTAAATGA CCAAACAGTA GGAATCCATG 
     2281 AGAATGGGAG AGAGAAGAAA AGACCCAAGC TGGAAAATCT GCCAGATACA CAAGACCAGC 
     2341 AAACTGTGGA TGTTAATTCA GTCAGTGAAG AAAATGAGAA TAATAGAGTA ACTTTAGAAA 
     2401 CGAACACTGA TTGTCTGAAA CCAGAGGAAG ACAGAAATAA AGAAAACCAA GAGACACTTG 
     2461 AGAGTAAACT TATACAATCT GAAGAAATTA ATGACACACA TATTCAAACA ATGGACTTAG 
     2521 TTTCTCAAGA GACTGGAGAA AAAGAAGCAG ATTTTCAGGC AGTTGACAAT GAAGTTGGGC 
     2581 TTACAAAGGA AGAAACCCAA GAGAAATTAG GAAAAGATGG TACAGCTCAA AAAGTTATAA 
     2641 CCAGTGATAG AAGCAGTGAG GTGGGGACAG ACGAGGCTCT AGATGACACT CAGAAGGCTG 
     2701 CTGAGCTCAG TAAGGCAGCA CAGAGTGGGG AAGGGGACGA AGCCCTGGCG CCTACCCAGA 
     2761 CACTAGCAGA GAAGCCCACA GAGGGCCCTG AGGCCGGTGG GGCTGAGGAA GAGCCTCCTG 
     2821 GTGGAGAGAG AGTTGAGGAT AAACAGCCAG AGCAGCAGCC TGCAGTGTGT GAAGCTGAGG 
     2881 GACAGTTAAC CAGCACGTCA GAGACCACAC GGGCAACCCT GGAGCAACCG GAGACGGATG 
     2941 AAGTTGAGCA GGTCAGCGAG TCCAATAGCA TTGAGGAGCT AGAGAGACTT GTAGTTACTG 
     3001 GTGCTGAGGC ACGGGCTCTC GGGAGTGAAG GTGAGGCAGC TGCTACTGAG GTAGATTTGG 
     3061 AGAGAAAAGA AAACGCACAG AAAGTGCCCG TTAAAGCAGA GTCCCAAGCT CCTGCAGCAT 
     3121 CGCAGCCCAG CGAGCCTCAC CCTGTCTTAA TACCCAGTAT TAATATTAAT TCTGAAACCA 
     3181 CAGAAAATAA AGAAGAAATG GGTGCTTTGC CAAAACCTGA AACCATACTG CCACCAGAGC 
     3241 CTGAACATGA AAAGGGAAAT GACACCGACT CAGGGACTGG GTCCACTGTG GAGAATAGCA 
     3301 GTGGTGACCT GAACTTGTCC ATCTCTAGCT TCCTAAGCAA AGCTAAGGAC AGCGGCTCAG 
     3361 TGTCTCTGCA GGAGACAAGA AGACAGAAGA AAACATTGAA GAAAACACGC AAGTTTATTG 
     3421 TCGATGGTGT AGAAGTGAGT GTGACGACAT CGAAGATAGT TACAGACAGC GACTCCAAAA 
     3481 CGGAGGAACT GCGCTTTCTC AGGCGTCAGG AACTTCGGGA GCTGAGGCTT CTTCAGAAGG 
     3541 AGGAGCAGCG AGCCCAGCAG CAGCTCAATG GGAAACTGCA GCAGCAGCGG GAGCAGATCT 
     3601 TCAGGCGCTT TGAGCAGGAG ATGCTGAGTA AGAAGCGACA ATATGACCAA GAAATTGAGA 
     3661 ATTTAGAGAA GCAGCAGAAA CAGACAATTG AACGGCTAGA ACAAGAGCAC ACTAACCGCC 
     3721 TGAGAGACGA AGCCAAGCGC ATCAAAGGAG AGCAGGAGAA GGAGCTGTCC AAGTTCCAGA 
     3781 ATGTGCTGAA AAACCGCAAG AAGGAGGAAC AAGAATTTGT TCAGAAGCAA CAACAAGAGT 
     3841 TAGATGGTTC TCTGAAAAAG ATTATCCAGC AGCAGAAGGC AGAGTTGGCC AATATTGAGA 
     3901 GAGAATGCCT GAATAACAAG CAGCAGCTCA TGAGAGCTCG AGAAGCCGCA ATTTGGGAGC 
     3961 TTGAAGAGCG ACATTTACAA GAGAAGCACC AGCTGCTTAA ACAGCAGCTT AAAGATCAGT 
     4021 ATTTCATGCA GAGACATCAG CTGCTAAAAC GCCATGAGAA GGAAACAGAA CAAATGCAGC 
     4081 GCTACAATCA ACGACTTATT GAAGAACTGA AAAACAGACA GACTCAGGAA CGAGCGAGAC 
     4141 TGCCCAAGAT TCAAAGAAGT GAAGCCAAGA CACGAATGGC CATGTTTAAA AAAAGTTTGA 
     4201 GGATCAACTC AACAGCCACA CCAGACCAGG ACCGTGAAAA AATTAAACAG TTTGCTGCAC 
     4261 AAGAAGAAAA GAGACAGAAA AATGAGAGAA TGGCTCAGCA TCAAAAACAT GAGAGCCAAA 
     4321 TGCGGGATCT TCAGTTGCAG TGTGAAGCCA ATGTTCGGGA ACTGCACCAG CTGCAGAATG 
     4381 AAAAATGCCA CCTGTTGGTT GAACATGAGA CTCAGAAGCT GAAGGAGTTG GATGAGGAGC 
     4441 ACAGCCAAGA GCTGAAGGAG TGGAGAGAGA AGCTGAGACC CAGGAAGAAG ACACTGGAAG 
     4501 AAGAGTTTGC CAGGAAACTG CAGGAACAGG AAGTGTTCTT TAAAATGACT GGGGAGTCCG 
     4561 AATGTCTTAA TCCATCAGCA CAGAGCCGGA TCTCTAAATT CTACCCTATT CCCACCTTAC 
     4621 ATTCCACTGG GTCAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4681 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4741 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4801 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4861 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4921 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4981 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5041 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5101 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5161 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5221 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5281 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5341 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5401 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5461 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5521 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5581 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5641 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5701 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5761 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5821 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5881 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5941 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6001 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6061 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6121 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6181 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6241 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6301 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6361 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6421 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6481 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6541 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6601 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6661 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6721 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6781 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6841 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6901 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6961 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7021 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7081 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7141 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7201 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7261 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7321 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7381 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7441 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7501 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7561 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7621 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7681 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7861 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7921 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7981 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8041 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8101 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8161 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8221 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8281 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8341 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8401 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8461 ATTTAACAAA AATTTAACGC GAATTTT
//