LOCUS       MR224375                9219 bp    DNA     circular  SYN 
DEFINITION  Ptpru (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, U (Ptpru), transcript variant 2
ACCESSION   NM_001083119
VERSION     NM_001083119.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5376..5467 
                     /label="Myc-DDK"  
     misc_feature    5497..6082 
                     /label="PolyA signal"  
     misc_feature    5554..5573 
                     /label="Restriction Enzyme"  
     misc_feature    6358..7030 
                     /label="ColE1"  
     misc_feature    7460..8254 
                     /label="Kan/Neo"  
     misc_feature    8289..8591 
                     /label="SV40 ori"  
     misc_feature    8677..9154 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5358..5364 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5358 
                     /label="Ptpru"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGGGCT CAGGCTCTGG TCCTGGCGCT CACCTTCCAG TTCTGCGCGC 
     1081 CTGAGACCGA GACTCCCGCA GCTGGCTGCA CCTTCGAGGA GGCGAGTGAC CCGGTCGTGC 
     1141 CCTGCGAGTT CAGCCAGGCT CAGTATGACG ACTTCCAATG GGAGCAAGTG CGGATCCACC 
     1201 CCGGCACCCG GACCCCTGAA GACCTGCCCC ATGGTGCCTA CTTGATGGTC AATGCTTCTC 
     1261 AGCATGCCCC AGGTCAGAGG GCCCACATCA TCTTCCAGAC CCTGAGCGAG AACGACACCC 
     1321 ATTGTGTGCA GTTCAGCTAC TTCCTGTACA GCAGGGATGG GCACAGCCCA GGCACCCTGG 
     1381 GGGTCTACGT GCGCGTGAAT GGGGGCCCTC TGGGCAGTGC CGTGTGGAAT ATGACCGGAT 
     1441 CCCACGGCCG TCAGTGGCAC CAGGCTGAGC TGGCTGTCAG CACCTTCTGG CCCAATGAGT 
     1501 ATCAGGTGCT GTTTGAGGCC CTCATCTCCC CAGACCACAA GGGCTACATA GGCTTAGACG 
     1561 ACATCTTGCT CTTCAGCTAT CCCTGCGCAA AGGCCCCTCA CTTCTCCCGC CTTGGGGACG 
     1621 TGGAGGTCAA TGCAGGCCAG AACGCATCCT TCCAATGCAT GGCAGCAGGC AGAGCCGCAG 
     1681 AGGCAGAACA CTTCTTCCTG CAGCGTCAGA GTGGAGTGCT GGTGCCTGCG GCCGGGGTGC 
     1741 GGCACATCAG CCACCGTCGC TTCCTGGCCA CTTTTCCGCT GGCCTCGGTA GGCCGCTCAG 
     1801 AGCAGGATCT GTACCGTTGC GTGTCCCAGG CCCCGCGTGG TGCTGGCGTC TCCAACTTTG 
     1861 CAGAGCTCAT CGTCAAAGAG CCTCCCACCC CCATCGCGCC CCCACAGCTG CTGCGTGCAG 
     1921 GCCCCACCTA CCTCATTATC CAGCTCAACA CCAACTCCAT CATTGGCGAC GGGCCGATCG 
     1981 TGCGCAAGGA GATCGAGTAC CGCATGGCAC GGGGCCCGTG GGCCGAGGTG CACGCTGTCA 
     2041 ACCTGCAGAC CTACAAGCTG TGGCATCTGG ACCCAGACAC TGAGTATGAA ATCAGCGTGC 
     2101 TGCTCACACG CCCGGGAGAT GGAGGCACAG GCCGCCCTGG GCCACCACTG ATCAGCCGGA 
     2161 CCAAGTGCGC AGAGCCCACG AGGGCCCCCA AAGGTCTGGC TTTTGCTGAG ATCCAGGCTC 
     2221 GCCAGCTGAC CCTGCAGTGG GAGCCCCTGG GCTATAATGT CACACGTTGT CATACCTACG 
     2281 CTGTGTCCCT TTGCTATCGC TACACCCTGG GCGGCAGCCA CAACCAGACC ATCCGGGAGT 
     2341 GTGTGAAGAT GGAGCGGGGT GCCAGCCGCT ACACCATCAA GAATCTGCTG CCATTCAGAA 
     2401 ACATCCACGT GCGTCTGATT CTCACAAACC CTGAGGGGCG CAAGGAGGGC AAGGAGGTCA 
     2461 CCTTCCAGAC AGATGAAGAT GTGCCTGGTG GGATTGCAGC TGAGTCCCTA ACCTTCACTC 
     2521 CACTGGAGGA CATGATCTTT CTCAAGTGGG AGGAGCCCCA GGAGCCCAAT GGCCTCATCA 
     2581 CTCAGTATGA GATCAGCTAC CAAAGCATTG AGTCCTCAGA CCCAGCAGTG AACGTGCCCG 
     2641 GCCCGAGACG CACCATCTCC AAACTCCGGA ATGAGACTTA CCACGTCTTC TCCAACCTGC 
     2701 ATCCCGGCAC CACGTATCTG TTCTCCGTGC GTGCTCGGAC GAGCAAGGGC TTCGGCCAGG 
     2761 CGGCTCTCAC TGAGATAACC ACCAACATCT CAGCTCCCAG CTTTGATTAT GCCGACATGC 
     2821 CGTCACCCCT GGGCGAGTCC GAGAACACCA TCACTGTGCT GTTGAGGCCG GCCCAGGGCC 
     2881 GAGGAGCCCC CATCAGCGTC TACCAGGTGG TTGTGGAGGA AGAGCGGCCA CGGCGCTTGC 
     2941 GGCGGGAGCC CGGAGCTCAG GACTGCTTCT CGGTACCTCT GACCTTTGAG ACGGCCCTGG 
     3001 CTCGCGGCCT GGTGCACTAC TTTGGGGCTG AACTGGCTGC CAGCAGCCTG CTTGAGGCCA 
     3061 TGCCCTTCAC CGTGGGTGAC AACCAGACCT ATCGTGGCTT CTGGAACCCA CCGCTTGAGC 
     3121 CCAGAAAGGC CTATCTCATC TATTTCCAGG CAGCAAGCCA CCTGAAAGGG GAAACCCGAC 
     3181 TGAACTGCAT CCGAATTGCC AGGAAAGCTG CGTGCAAGGA GAGCAAGCGA CCCCTCGAAG 
     3241 TGTCCCAGAG ATCGGAGGAG ATGGGGCTCA TCCTGGGCAT CTGTGCAGGT GGTCTTGCCG 
     3301 TCCTCATTCT CCTCCTGGGG GCCATCATTG TCATCATCCG CAAAGGGAGG GACCGCTATG 
     3361 CCTACTCTTA CTACCCGAAG CCAGTGAACA TGACGAAAGC CACGGTCAAC TACCGCCAGG 
     3421 AGAAGACTCA CATGATGAGT GCCGTGGACC GCAGCTTCAC AGATCAGAGT ACTCTGCAGG 
     3481 AGGATGAGCG GTTGGGTCTG TCCTTTATGG ATGCTCCTGG CTATAGTCCT CGTGGAGACC 
     3541 AGCGAAGCGG TGGTGTCACC GAGGCCAGCA GCCTCCTGGG GGGTTCTCCA AGGCGCCCAT 
     3601 GCGGCCGGAA GGGTTCTCCG TATCATACCG GGCAGCTCCA CCCTGCAGTC CGAGTGGCTG 
     3661 ACCTTCTACA GCACATCAAC CAGATGAAGA CAGCCGAGGG CTACGGCTTC AAGCAGGAGT 
     3721 ACGAGAGTTT CTTTGAGGGC TGGGACGCCA CCAAGAAGAA AGACAAGCTC AAGGGCGGCC 
     3781 GACAGGAGCC AGTGTCTGCC TATGATCGAC ACCATGTGAA ACTACACCCG ATGCTGGCAG 
     3841 ACCCTGATGC CGACTACATC TCTGCCAACT ACATAGACGG CTACCACAGG TCAAACCACT 
     3901 TCATAGCCAC TCAAGGGCCA AAGCCTGAGA TGATCTACGA TTTCTGGCGC ATGGTGTGGC 
     3961 AGGAACAGTG TGCGAGCATC GTCATGATCA CCAAGCTGGT AGAGGTGGGC AGGGTGAAGT 
     4021 GTTCTCGCTA CTGGCCTGAG GACTCAGACA TGTATGGGGA CATCAAGATC ACGCTGGTAA 
     4081 AGACAGAGAC ACTGGCTGAG TATGTGGTGC GCACCTTTGC CCTGGAGCGG AGAGGTTACT 
     4141 CAGCCCGGCA TGAGGTCCGC CAGTTCCATT TCACAGCGTG GCCAGAGCAT GGTGTCCCCT 
     4201 ACCACGCCAC GGGGCTGCTG GCCTTCATCC GGCGTGTGAA GGCTTCCACT CCACCTGATG 
     4261 CCGGGCCCAT TGTCATTCAC TGCAGTGCAG GAACTGGCCG CACAGGCTGC TACATCGTCC 
     4321 TGGATGTGAT GCTGGACATG GCTGAATGTG AGGGGGTCGT GGACATTTAC AACTGTGTGA 
     4381 AGACCCTCTG TTCCCGACGG GTCAACATGA TCCAGACGGA GGAACAATAT ATCTTCATCC 
     4441 ACGATGCAAT CTTGGAGGCC TGCCTGTGTG GGGAGACCAC CATCCCTGTC AACGAGTTCA 
     4501 AGGCCACCTA CAGGGAGATG ATCCGCATTG ACCCTCAGAG CAATTCCTCC CAGCTTCGGG 
     4561 AAGAGTTCCA GACGCTGAAC TCGGTCACGC CGCCGCTGGA TGTGGAGGAG TGTAGCATTG 
     4621 CCCTGCTGCC CCGGAATCGA GACAAGAACC GTAGCATGGA TGTGCTGCCA CCAGACCGCT 
     4681 GCCTGCCCTT CCTCATCTCC AGTGATGGGG ACCCCAATAA CTACATCAAT GCAGCACTGA 
     4741 CTGACAGCTA CACACGGAGC GCCGCCTTCA TCGTGACCCT GCACCCGCTG CAGAGTACCA 
     4801 CGCCCGACTT CTGGCGGCTG GTCTACGACT ACGGGTGCAC CTCCATCGTC ATGCTGAACC 
     4861 AACTTAACCA GTCCAACTCC GCCTGGCCCT GCTTGCAGTA CTGGCCGGAG CCAGGCCGAC 
     4921 AGCAGTATGG GCTCATGGAG GTGGAGTTTG TGTCTGGCAC AGCAAACGAG GATTTGGTGT 
     4981 CCCGAGTGTT CCGGGTGCAG AACTCTTCTC GGCTGCAGGA GGGTCACCTG CTGGTACGGC 
     5041 ACTTCCAGTT TCTGCGTTGG TCTGCTTATC GGGACACGCC TGACTCCAGG AAGGCCTTTC 
     5101 TGCACCTGTT GGCTGAGGTG GACAAGTGGC AGGCAGAGAG TGGGGATGGG CGCACCGTGG 
     5161 TGCATTGTCT CAACGGGGGT GGCCGCAGTG GCACCTTCTG CGCCTGTGCC ACGGTCTTGG 
     5221 AGATGATCCG CTGTCACAGC CTGGTGGATG TTTTCTTTGC TGCCAAAACA CTTCGGAACT 
     5281 ACAAGCCCAA TATGGTGGAG ACCATGGATC AGTATCATTT CTGCTACGAC GTGGCCCTGG 
     5341 AGTACCTGGA GGCTCTGGAG TTGAGAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5401 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5461 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5521 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5581 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5641 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5701 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5761 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5821 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5881 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5941 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     6001 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6061 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6121 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6181 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6241 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6301 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6361 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6421 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6481 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6541 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6601 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6661 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6721 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6781 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6841 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6901 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6961 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     7021 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7081 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7141 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7201 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7261 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7321 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7381 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7441 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7501 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7561 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7621 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7681 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7741 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7801 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7861 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7921 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7981 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     8041 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8101 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8161 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8221 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8281 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8341 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8401 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8461 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8521 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8581 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8641 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8701 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8761 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8821 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8881 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8941 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     9001 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9061 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9121 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9181 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//