LOCUS       MR224361                8436 bp    DNA     circular  SYN 
DEFINITION  Ptch2 (Myc-DDK-tagged) - Mouse patched homolog 2 (Ptch2)
ACCESSION   NM_008958
VERSION     NM_008958.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4591..4682 
                     /label="Myc-DDK"  
     misc_feature    4712..5297 
                     /label="PolyA signal"  
     misc_feature    4769..4788 
                     /label="Restriction Enzyme"  
     misc_feature    5573..6245 
                     /label="ColE1"  
     misc_feature    6675..7469 
                     /label="Kan/Neo"  
     misc_feature    7504..7806 
                     /label="SV40 ori"  
     misc_feature    7892..8369 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4566..4573 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4566 
                     /label="Ptch2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTCGGCCA CTGTCCCTTG GAGAGCTACC TCCCAGCTAC ACACCTCCAG 
     1081 CTCGGTCCTC CGCACCTCAT ATCCTAGCTG GGAGCCTGCA GGCTCCTCTC TGGCTTCGAG 
     1141 CTTACTTCCA AGGTCTACTC TTCTCCCTGG GGTGCAGGAT CCAGAAACAC TGCGGCAAAG 
     1201 TGCTCTTCCT GGGACTGGTG GCCTTTGGGG CTCTGGCACT GGGTCTCCGA GTGGCTGTAA 
     1261 TTGAGACAGA CCTAGAACAG CTCTGGGTAG AAGTGGGCAG CAGGGTGAGC CAGGAGCTGC 
     1321 ACTACACCAA GGAGAAGCTG GGGGAAGAGG CTGCGTACAC CTCCCAGATG TTGATTCAGA 
     1381 CTGCGCACCA GGAAGGGGGA AACGTCCTCA CCCCCGAGGC ACTTGACTTG CACCTCCAGG 
     1441 CGGCGCTCAC TGCCAGTAAA GTCCAAGTAT CACTCTATGG GAAATCCTGG GATTTGAACA 
     1501 AGATCTGCTA CAAATCAGGG GTTCCCCTTA TTGAAAATGG GATGATCGAG CGGATGATTG 
     1561 AGAAGCTGTT TCCCTGTGTG ATCCTCACCC CGCTTGACTG CTTCTGGGAA GGAGCCAAAC 
     1621 TCCAAGGGGG CTCTGCCTAC TTGCCGGGCC GCCCTGATAT CCAGTGGACC AACCTGGACC 
     1681 CTCAGCAGCT GCTCGAGGAG CTGGGCCCCT TTGCCTCTCT GGAGGGCTTC CGGGAACTGC 
     1741 TAGATAAGGC ACAGGTGGGC CAGGCCTATG TGGGGCGACC CTGTCTGGAC CCTGATGACC 
     1801 CCCACTGTCC ACCTAGTGCT CCCAACCGGC ACAGCAGGCA GGCTCCCAAT GTGGCTCAGG 
     1861 AGCTGAGTGG GGGCTGCCAT GGCTTCTCCC ACAAGTTCAT GCACTGGCAG GAGGAACTGC 
     1921 TACTAGGGGG CACAGCCAGA GATCTCCAAG GACAGCTGCT GAGGGCAGAG GCCTTGCAGA 
     1981 GCACCTTCCT GCTCATGAGT CCCCGTCAGC TGTACGAGCA CTTCCGGGGC GACTACCAGA 
     2041 CACATGACAT CGGCTGGAGC GAGGAGCAGG CCAGCATGGT GCTGCAGGCC TGGCAGAGGC 
     2101 GCTTTGTGCA GCTAGCCCAG GAGGCTCTGC CGGCCAACGC GTCCCAGCAG ATCCATGCCT 
     2161 TCTCCTCCAC CACCCTGGAT GACATCCTGC GCGCGTTCTC TGAAGTCAGC ACCACCCGTG 
     2221 TGGTAGGAGG CTATCTGCTT ATGCTGGCCT ATGCCTGCGT AACAATGCTG CGGTGGGACT 
     2281 GCGCCCAGTC CCAGGGTGCC GTAGGTCTTG CTGGGGTGTT GCTGGTGGCC CTGGCGGTGG 
     2341 CCTCAGGTCT TGGGCTTTGT GCCCTACTTG GCATCACTTT CAATGCTGCC ACTACACAGG 
     2401 TGCTGCCCTT CTTGGCTCTG GGCATTGGCG TGGATGACAT CTTCCTGCTG GCGCATGCCT 
     2461 TCACAAAAGC CCCACCTGAC ACCCCTCTCC CAGAGCGTAT GGGTGAGTGT TTGAGGAGCA 
     2521 CGGGCACCAG CGTGGCACTC ACATCCGTCA ACAACATGGT CGCCTTTTTC ATGGCTGCCC 
     2581 TGGTTCCCAT TCCTGCGCTG CGGGCCTTCT CCCTGCAGGC AGCCATAGTG GTCGGCTGCA 
     2641 ACTTTGCAGC GGTAATGCTC GTCTTCCCTG CCATCCTCAG CCTCGACCTG CGCCGGCGCC 
     2701 ACCGCCAACG CCTGGACGTT CTCTGCTGCT TTTCTAGCCC CTGCTCCGCT CAGGTCATTC 
     2761 AGATGCTGCC CCAGGAGCTA GGGGACAGAG CAGTACCAGT GGGCATTGCC CACCTGACTG 
     2821 CCACCGTGCA AGCCTTCACC CACTGCGAAG CCAGCAGCCA GCATGTAGTC ACCATTTTGC 
     2881 CTCCTCAAGC CCACCTGCTG TCTCCAGCTT CTGACCCACT GGGCTCCGAA CTCTATAGCC 
     2941 CTGGAGGGTC TACACGGGAC CTTCTCAGTC AGGAGGAGGG GACAGGGCCA CAGGCGGCCT 
     3001 GCAGGCCCCT GCTCTGTGCC CACTGGACTC TCGCCCATTT TGCCCGCTAT CAGTTTGCAC 
     3061 CTTTACTGCT CCAGACACGA GCCAAGGCCC TGGTGTTGCT GTTCTTTGGG GCTCTTTTGG 
     3121 GCCTGAGCCT CTATGGAGCC ACCTTGGTAC AAGATGGGCT GGCCCTGACA GATGTGGTCC 
     3181 CTAGGGGCAC CAAGGAACAT GCTTTCCTGA GCGCCCAGCT CAGGTACTTC TCCCTGTACG 
     3241 AGGTGGCTCT AGTGACACAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC GCCCTCTTTG 
     3301 ATCTGCACCA GCGCTTCAGC TCCCTCAAGG CTGTGCTGCC CCCACCTGCC ACCCAGGCAC 
     3361 CCCGCACCTG GCTTCACTAC TACCGCAGCT GGCTACAGGG TATCCAGGCT GCATTTGACC 
     3421 AAGACTGGGC TTCTGGGCGC ATCACCTGCC ACTCTTACCG CAACGGCTCA GAAGATGGCG 
     3481 CCCTGGCCTA CAAGCTGCTC ATCCAAACCG GGAATGCCCA GGAGCCTCTG GATTTCAGCC 
     3541 AGCTGACCAC AAGGAAACTG GTGGACAAGG AGGGACTCAT TCCCCCAGAG CTCTTCTACA 
     3601 TGGGGCTAAC CGTGTGGGTG AGCAGCGACC CCCTGGGCCT CGCAGCCTCT CAGGCCAACT 
     3661 TCTACCCCCC ACCTCCAGAG TGGCTTCACG ACAAATATGA TACCACCGGG GAGAACCTTC 
     3721 GCATCCCGGC AGCCCAGCCC TTGGAGTTTG CCCAGTTCCC CTTCCTGCTG CATGGACTCC 
     3781 AGAAGACTGC AGACTTTGTA GAAGCCATCG AAGGGGCCAG GGCGGCATGC ACAGAGGCAG 
     3841 GCCAGGCAGG GGTGCATGCC TACCCCAGTG GCTCCCCCTT CCTCTTCTGG GAGCAGTATC 
     3901 TGGGTCTTCG GCGCTGCTTC CTGCTGGCAG TCTGCATCTT GCTGGTGTGC ACCTTCCTCG 
     3961 TCTGTGCCCT GCTTCTACTC AGCCCGTGGA CAGCTGGCCT CATAGTGCTG GTCCTGGCAA 
     4021 TGATGACTGT GGAGCTCTTT GGTATCATGG GATTCCTGGG CATCAAACTG AGTGCCATCC 
     4081 CCGTGGTAAT CCTCGTGGCC TCTATAGGCA TTGGTGTTGA ATTCACAGTT CACGTGGCTC 
     4141 TGGGCTTCCT GACCAGCCAT GGTAGCCGGA ACCTGCGGGC TGCTAGCGCT CTGGAACAGA 
     4201 CCTTTGCCCC TGTGACTGAT GGAGCTGTCT CCACCTTGCT GGGTCTGCTC ATGCTTGCTG 
     4261 GTTCTAACTT TGACTTCATC ATAAGGTATT TCTTTGTGGT GCTGACGGTG CTGACACTCT 
     4321 TGGGCCTGCT CCATGGACTC CTGCTGCTGC CTGTGCTGCT CTCTATCCTA GGCCCCCCGC 
     4381 CACAGGTGGT GCAGGTATAC AAGGAGAGTC CACAGACCCT AAACTCCGCT GCTCCACAGA 
     4441 GAGGTGGGCT CAGGTGGGAC AGGCCCCCCA CCCTGCCCCA GAGTTTTGCC AGAGTGACTA 
     4501 CCTCCATGAC TGTGGCCCTC CACCCACCAC CTCTGCCTGG AGCATACGTC CACCCAGCCT 
     4561 CCGAGGAGCC CACAAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4621 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4681 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4741 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4801 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4861 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4921 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4981 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5041 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5101 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5161 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5221 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5281 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5341 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5401 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5461 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5521 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5581 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5641 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5701 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5761 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5821 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5881 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5941 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6001 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6061 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6121 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6181 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6241 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6301 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6361 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6421 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6481 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6541 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6601 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6661 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6721 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6781 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6841 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6901 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6961 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7021 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7081 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7141 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7201 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7261 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7321 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7381 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7441 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7501 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7561 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7621 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7681 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7801 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7861 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7921 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7981 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8041 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8101 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8161 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8221 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8281 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8341 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8401 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//