LOCUS       MR224327                7335 bp    DNA     circular  SYN 
DEFINITION  Clcn3 (Myc-DDK-tagged) - Mouse chloride channel 3 (Clcn3), transcript variant b
ACCESSION   NM_173873
VERSION     NM_173873.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3492..3583 
                     /label="Myc-DDK"  
     misc_feature    3613..4198 
                     /label="PolyA signal"  
     misc_feature    3670..3689 
                     /label="Restriction Enzyme"  
     misc_feature    4474..5146 
                     /label="ColE1"  
     misc_feature    5576..6370 
                     /label="Kan/Neo"  
     misc_feature    6405..6707 
                     /label="SV40 ori"  
     misc_feature    6793..7270 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3474..3480 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3474 
                     /label="Clcn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCTGAG CAGCTGTTCC ATAGAGGCTA CTATAGAAAC AGCTACAACA 
     1081 GCATAACCAG CGCGAGTAGC GATGAGGAGC TCCTAGATGG AGCAGGTGCC ATTATGGACT 
     1141 TTCAGACTTC TGAAGATGAC AATTTGTTAG ACGGGGACAC AGCAGCTGGA ACTCATTATA 
     1201 CAATGACAAA TGGAGGCAGC ATTAATAGCT CTACACACTT GCTGGATCTT TTGGATGAGC 
     1261 CTATCCCAGG TGTCGGTACC TACGATGATT TCCATACTAT TGACTGGGTG CGAGAGAAGT 
     1321 GTAAGGACAG AGAAAGGCAC AGACGGATCA ACAGTAAAAA AAAAGAATCA GCATGGGAAA 
     1381 TGACAAAAAG TCTGTATGAC GCCTGGTCAG GATGGCTTGT TGTTACACTG ACGGGACTGG 
     1441 CATCAGGGGC ACTAGCTGGA TTGATAGACA TTGCTGCTGA CTGGATGACT GACCTGAAGG 
     1501 AGGGCATCTG CCTCAGTGCA TTGTGGTACA ACCATGAACA GTGTTGTTGG GGCTCTAATG 
     1561 AGACAACGTT TGAAGAGAGG GATAAATGTC CACAGTGGAA AACATGGGCA GAGTTAATCA 
     1621 TTGGCCAAGC AGAGGGCCCT GGATCTTATA TCATGAACTA CATCATGTAC ATCTTTTGGG 
     1681 CTTTGAGTTT TGCCTTTCTT GCAGTTTCTT TGGTGAAAGT ATTTGCTCCA TATGCCTGTG 
     1741 GCTCTGGAAT TCCAGAGATT AAAACTATTT TGAGTGGATT TATCATCAGA GGATACTTGG 
     1801 GAAAATGGAC TTTAATGATT AAAACTATCA CGTTAGTGCT GGCTGTGGCA TCAGGTTTGA 
     1861 GTTTAGGAAA AGAAGGTCCC CTGGTACATG TTGCCTGCTG CTGTGGAAAC ATCTTTTCCT 
     1921 ACCTCTTTCC AAAGTATAGC ACCAATGAAG CTAAAAAGAG GGAGGTGCTG TCAGCCGCCT 
     1981 CAGCTGCTGG GGTTTCTGTG GCTTTTGGTG CACCGATCGG AGGAGTTCTT TTTAGCTTGG 
     2041 AGGAGGTTAG CTATTATTTT CCTCTCAAAA CTTTATGGAG ATCATTTTTT GCTGCTTTGG 
     2101 TGGCAGCATT TGTTTTGAGA TCCATCAATC CATTTGGTAA CAGCCGTCTG GTCCTCTTTT 
     2161 ATGTGGAGTA TCATACACCA TGGTACCTTT TTGAACTGTT TCCTTTTATT CTCCTAGGGG 
     2221 TATTTGGAGG GCTTTGGGGA GCTTTTTTTA TTAGGGCAAA TATTGCCTGG TGTCGTCGAC 
     2281 GCAAGTCCAC CAAATTTGGA AAGTATCCTG TTCTCGAAGT CATTATTGTT GCAGCCATTA 
     2341 CTGCTGTGAT AGCCTTCCCC AACCCATACA CAAGGCTCAA CACCAGTGAA CTGATTAAAG 
     2401 AGCTGTTTAC AGATTGTGGG CCGTTGGAAT CCTCCTCTCT CTGTGACTAC AGAAATGACA 
     2461 TGAATGCCAG TAAAATTGTT GACGATATTC CTGACCGTCC AGCAGGCGTT GGAGTATATT 
     2521 CAGCTATCTG GCAGTTGTGC CTAGCGCTCA TATTTAAAAT AATAATGACA GTATTCACTT 
     2581 TTGGTATCAA GGTCCCGTCA GGCTTGTTTA TCCCCAGCAT GGCCATTGGA GCCATTGCAG 
     2641 GGAGAATTGT GGGGATCGCT GTGGAGCAGC TTGCCTACTA TCACCACGAC TGGTTTATCT 
     2701 TCAAGGAGTG GTGTGAGGTT GGGGCTGACT GCATCACTCC CGGGCTGTAT GCCATGGTTG 
     2761 GGGCTGCTGC GTGCTTAGGT GGTGTGACAA GAATGACTGT GTCTCTGGTG GTTATTGTTT 
     2821 TTGAACTTAC TGGAGGCTTG GAATATATTG TTCCTCTTAT GGCTGCAGTA ATGACCAGTA 
     2881 AATGGGTTGG TGATGCCTTT GGTAGGGAAG GTATTTATGA AGCACACATC CGACTAAATG 
     2941 GGTACCCTTT CTTGGATGCA AAAGAAGAAT TCACTCATAC AACCCTGGCT GCTGATGTTA 
     3001 TGAGACCTCG AAGAAGTGAC CCTCCCTTAG CTGTTTTGAC ACAGGACAAT ATGACAGTAG 
     3061 ATGACATAGA AAACATGATT AATGAAACCA GCTATAATGG CTTTCCTGTT ATAATGTCAA 
     3121 AAGAATCTCA GAGATTAGTG GGATTTGCCC TCAGAAGAGA CCTGACTATT GCAATAGAAA 
     3181 GTGCCAGAAA AAAACAAGAA GGGATTGTTG GCAGTTCTCG GGTGTGTTTT GCACAGCATA 
     3241 CTCCATCTCT TCCAGCAGAA AGTCCACGGC CATTAAAACT GAGAAGCATC CTTGACATGA 
     3301 GCCCTTTTAC AGTGACAGAC CACACCCCAA TGGAGATTGT GGTAGACATC TTTCGAAAGC 
     3361 TTGGTCTGAG GCAGTGCCTT GTAACTCACA ACGGACGCCT CCTTGGCATT ATAACAAAAA 
     3421 AAGATATCCT CCGTCATATG GCCCAGACGG CAAACCAAGA CCCCGCTTCA ATAATGTTCA 
     3481 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3541 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3601 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3661 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3721 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3781 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3841 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3901 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3961 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4021 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4081 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4141 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4201 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4261 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4321 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4381 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4441 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4501 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4561 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4621 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4681 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4741 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4801 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4861 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4921 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4981 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5041 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5101 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5161 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5221 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5281 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5341 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5401 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5461 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5521 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5581 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5641 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5701 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5761 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5821 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5881 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5941 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6001 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6061 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6121 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6181 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6241 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6301 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6361 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6421 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6481 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6541 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6601 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6661 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6721 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6781 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6841 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6901 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6961 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7021 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7081 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7141 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7201 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7261 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7321 TTTAACGCGA ATTTT
//