LOCUS       MR224317                8565 bp    DNA     circular  SYN 
DEFINITION  Aff3 (Myc-DDK-tagged) - Mouse AF4/FMR2 family, member 3 (Aff3)
ACCESSION   NM_010678
VERSION     NM_010678.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4722..4813 
                     /label="Myc-DDK"  
     misc_feature    4843..5428 
                     /label="PolyA signal"  
     misc_feature    4900..4919 
                     /label="Restriction Enzyme"  
     misc_feature    5704..6376 
                     /label="ColE1"  
     misc_feature    6806..7600 
                     /label="Kan/Neo"  
     misc_feature    7635..7937 
                     /label="SV40 ori"  
     misc_feature    8023..8500 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4704..4710 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4704 
                     /label="Aff3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAGCTTC GACTTAGCCC TGCTCCAGGA ATGGGACCTC GAGTCGCTGT 
     1081 GTGTCTATGA ACCGGACAGG AATGCGTTAC GGAGGAAAGA GCGAGAAAGA AGAAGTCAAG 
     1141 AAACACAGCA GGACAGTGGC AGCTTTAACT CCGGTTACTC GCTCTTCAGC GAACCCTACA 
     1201 AGACGAACAA GGGGGATGAA CTCTCCAACC GGATCCAGAA TACATTAGGA AATTATGATG 
     1261 AGATGAAAGA CTTTTTAACT GATAGATCCA ACCAGAGTCA TCTTGTTGGC GTCCCCAAGC 
     1321 CAGGTGTCCC TCAGACTCCC GTGAACAAGA TAGATGAGCA TTTTGGGGCA GAGTCGAGAG 
     1381 CCCAGCCCCA GCCCTCAACC GTCTGCAGCA CTGCGTCCTC TACACCTGCA GCTGTCCCTG 
     1441 TGCAGCAGGG CAAGAGAGGT GCCATGGGCT GGCAGAAGGC TGGGCACCCA CCGTCCGATG 
     1501 GCCAACAGAG AGCAGCGCAA CAGGGCTCTC TCAGGACCTT GCTTGGAGAT GGTGTTGGCA 
     1561 GACAGCAGAC AAGGGCCAAG CAAGTGTGCA ATATGGAGAC GGGGCTTCAG ACCCAGGAGA 
     1621 GGCCACCTGC AATGGCAGCC AAGCACGGCG GCAGTGGACA CTGTGTTCAG AACTTTCCCC 
     1681 CATCCCTGGC TTCAAAACCC AGCCTGGTGC AGCAGAAACC AACTGCCTAC GTGCGGCCAA 
     1741 TGGATGGCCA GGACCAAGCT CCTGACGAAT CTCCTAAGCT GAAGTCATCC ACAGAAACTG 
     1801 CTGTGCACTG CACAGCCTAC AGAGGGGTCC CGGCCAACAA ACCAGAGTCA GCCCGAGCCA 
     1861 AGGCCAAGCT TGCCAAGTTC AGCATCCCCA AGCAGGCAGA GGAGAGTAGA TCTGGAGAAA 
     1921 ACAACAGCTG TGTGGAAGAA ATAATTCGGG AGATGACCTG GCTTCCTCCA CTTTCTGCTA 
     1981 TTCAAGCTCC TGCCAAAGTG GAACCAAGCA AGTTCCCATT CCCAAATAAG GACTCGCAAC 
     2041 TTGTATCCTC TGGACACAGT AATCCAAAGA AAGCTGATGC GGAGCCAGGG AGTCCAGACA 
     2101 ATGGCGCATC GAACACTTCA ACTCTGGAAG ACGACCTTAA GCTAAGCAGT GATGACGAAG 
     2161 AGGGTGAGCA GGCAGCCCAG AGAACAGCTC TCCGCGCTCT AGCTGACAGC TCCGTGGTTC 
     2221 AGCAGACCAA CTGTAGAGGT TCGGCGCCAT CCAGCAAGGG CGGCGGCAGC AGCAGCAGCA 
     2281 GCGGCGGCAG CAGCTCCTCC AGCGACTCAG AGAGCACTTC CGGCTCCGAC TCCGAGACGG 
     2341 AGAGCAGCTC CAGCTCCAGC GAGAGCGAGG GCAGCAAGCC TCCGCACTGC TCCAGCCCCG 
     2401 AGGCCGAACC TGCGTCCTCT AACAAGTGGC AGCTGGATAA ATGGCTAAAC AAAGTTAATC 
     2461 CTCACAAGCC GCCAATTCTG ATCCAAAATG AAAGCCACGG CCCGGAAAGG AATCAATACT 
     2521 ACACGCCGCC GGTGAAAGAT GAAGGGCAAG ACTGCGGGAA ACTTCCTGAG ATCTGCCAAG 
     2581 CCAGCCTGAG GGACAAGGAG CTCAAGACCA CCTGCAAGGA GGAGCAGAGG CCGAGGACCG 
     2641 CAAACAAGGC CCCTGGCAGC AAAAGTGTAA AGCAGAAGTC CCCACCCGCT GCTGTGGCTG 
     2701 TGACCGCAGC TGCCCTCCCG CCCGCGGTGC CCAGCGCACC CACGGAGAGC GCGCCTGCGC 
     2761 CCACCCGCAG GTCAGCAGGC AAGAAGCCCA CCCGGCGCAC CGAGAGGACC TCAGCCGGGG 
     2821 ACGGTGCCAA CTGCCACCGG CCTGAGGAGC CAGTGGCCCC AGACACGCTG GGGGCGAGCG 
     2881 TGGTGGGACC CCTAGAGCCC CCCAAAACCA GACCCTGCGG TAACAACAGG ACAGGCCACC 
     2941 GCAAGGAGCT GCGCTCCTCC GTGACCTGTG AGAAGCGGCG CACGAGGGGG CTGAGCAGAA 
     3001 TCGTCCCCAA ATCCAAGGAG TTTATTGAAA CAGAATCTTC GTCTTCCTCT TCCTCCTCGG 
     3061 ACTCAGACCT GGAGTCAGAG CAAGAGGAGT ACGTCCTGTC CAAGGCTCCG ACCACCACTG 
     3121 GGAGCGAGCA GCGGCTGAAG GAGGCCGCCA GCAGCAACAA CAACAGCAAC AGCAACAGCA 
     3181 GCACCTCCCG GGCTTCTGTG GGCTCCATCA ACGCCAGGAC CACCAGTGAC ATTGCCAAGG 
     3241 AGCTAGAGGA ACAGTTCTAC ACGCTGGTCC CCTTTGGCAG GAATGAACTA CTATCTCCGC 
     3301 TAAAGGACAG CGATGAGGTC AGGTCTCTCT GGGTCAAAAT TGACCTGACC CTCCTGTCCA 
     3361 GGATCCCAGA ACACCTGTCC CAGGAGCCTG GGGTTTTGAG TGCTCCTTCG GCCAAGGACA 
     3421 CTGATAGTGC CCCAGCCAGT CATGCATTAG ACGCTCCTGC TGAAAAGACT TTGCCAAAAT 
     3481 CCAAGAGGAA ACGCAAGTGC GACAACGAAG ATGACTACAG GGAGATAAAG AAGGTCCAGG 
     3541 GAAGGAAGGA GAGTGCCTCA CGCCTCGCCG CCTCCACTAA TAACACCTTG TCCGGCAATC 
     3601 ACTGCAATGT GAACGTCAAC AGCTTGGCAA TACCAATAAA CAAAAATGAA AAGATGCTCC 
     3661 GGTCGCCCAC TTCGCCACTC TCAGACACAT GTAAACACAA ATACGCCAGC GAGGACCTGA 
     3721 CTTCTTCCAG CCGACCTCAC GGCAACGGGT TGTTGACTTC AGCGTCTTCC AACAAAGAGC 
     3781 CAAAGGCTGA GAGTCAGCTG CAGACGATTG CTGGGGACCT CACGAAGGCA TCTCACAACA 
     3841 GTTCTGAAAA TGGCACCCTC CATAGCAAGT CACGGCCCCA GACGGAGCCA TGGTCTCCAG 
     3901 GTTCCAATGG CCACAGGGAC TGCAAGAGAC AGAAACTGAT CTTCGATGAC ATGCCTCGAA 
     3961 GTGCAGATTA TTTTATGCGA GAAGCCAAGC GGATGAAACA TAAAGCAGAC GCCATGGTGG 
     4021 AGAAGTTCGG GAAGGCCCTG AACTATGCCG AAGCTGCCTT GTCCTTCATC GAGTGCGGAA 
     4081 ACGCAATGGA ACAAGGGCCA ATGGAATCCA AGTCTCCCTA CACCATGTAC TCGGAGACAG 
     4141 TGGAGCTCAT CAGGTATGCT ATGAGACTAA AAACCCACTC AGGCCCCAAT GCCACACCAG 
     4201 AGGACAAACA GTTAGCTGCG CTTTGTTACC GATGCCTGGC TCTCCTATAC TGGCGGATGT 
     4261 TTCGACTCAA AAGAGACCAT GCTGTAAAAT ATTCAAAAGC ACTTATCGAC TATTTCAAGA 
     4321 ATTCATCGAA AGCTGCCCAA GCCCCATCCC CATGGGGGTC CAGTGGCAAG AGCACTGGAA 
     4381 GCCCATCCCC CATGTCTCCC AACCCTTCTC CCGCCAGCTC CGTGGGGTCT CAGGGCAGCC 
     4441 TCTCCAGCTC CAGCGGCCTG TCCCCATCGA CCATCGTCAG CATCCCCCAG CGCATTCACC 
     4501 AGATGGCGGC CAATCACGTC AGCATCACCA ACAGCATCCT CCACAGCTAT GACTACTGGG 
     4561 AGATGGCTGA TAACCTGGCC AAGGAAAACA GAGAGTTCTT CAATGACCTG GATTTGCTCA 
     4621 TGGGACCTGT CACCCTGCAC AGCAGCATGG AACACCTGGT CCAGTACTCC CAGCAAGGCC 
     4681 TGCATTGGCT TCGGAGCAGC GTACACCTGT CCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4741 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4801 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4861 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4921 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4981 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5041 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5101 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5161 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5221 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5281 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5341 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5401 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5461 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5521 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5581 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5641 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5701 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5761 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5821 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5881 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5941 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6001 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6061 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6121 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6181 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6241 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6301 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6361 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6421 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6481 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6541 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6601 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6661 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6721 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6781 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6841 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6901 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6961 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7021 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7081 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7141 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7201 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7261 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7321 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7381 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7441 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7501 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7561 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7621 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7681 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7741 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7801 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7861 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7921 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7981 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8041 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8101 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8161 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8221 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8281 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8341 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8401 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8461 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8521 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//