LOCUS       MR224301                7716 bp    DNA     circular  SYN 
DEFINITION  Unc5b (Myc-DDK-tagged) - Mouse unc-5 homolog B (C. elegans) (Unc5b)
ACCESSION   NM_029770
VERSION     NM_029770.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3873..3964 
                     /label="Myc-DDK"  
     misc_feature    3994..4579 
                     /label="PolyA signal"  
     misc_feature    4051..4070 
                     /label="Restriction Enzyme"  
     misc_feature    4855..5527 
                     /label="ColE1"  
     misc_feature    5957..6751 
                     /label="Kan/Neo"  
     misc_feature    6786..7088 
                     /label="SV40 ori"  
     misc_feature    7174..7651 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3855..3861 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3855 
                     /label="Unc5b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGGCCCGG AGCGGGGTGC GGAGCGCGCT GCTGCTGGCG CTGCTGCTTT 
     1081 GCTGGGATCC GACACCGAGC CTAGCAGGCG TTGACTCTGC TGGCCAGGTG CTCCCAGACT 
     1141 CCTACCCATC AGCCCCTGCG GAGCAGCTGC CGTACTTCCT ATTGGAGCCA CAGGACGCCT 
     1201 ACATCGTAAA GAACAAGCCA GTGGAACTGC ACTGCAGAGC CTTCCCTGCC ACGCAGATCT 
     1261 ACTTCAAGTG TAATGGCGAG TGGGTCAGCC AGAATGACCA CGTCACACAG GAGAGCCTGG 
     1321 ATGAGGCCAC AGGCTTGCGG GTGCGAGAGG TGCAGATCGA GGTGTCACGG CAGCAAGTGG 
     1381 AGGAACTCTT CGGGCTCGAG GACTACTGGT GCCAGTGCGT GGCCTGGAGC TCTTCGGGAA 
     1441 CTACCAAGAG TCGCCGAGCC TACATCCGCA TTGCCTACTT GCGCAAGAAC TTTGACCAGG 
     1501 AGCCTCTGGC CAAGGAGGTA CCCTTGGATC ATGAGGTCCT TCTGCAGTGC CGCCCACCGG 
     1561 AGGGAGTGCC TGTGGCTGAG GTGGAATGGC TCAAGAATGA AGATGTCATT GACCCCGCTC 
     1621 AGGACACTAA CTTCCTGCTC ACCATTGACC ACAACCTCAT CATCCGCCAG GCGCGCCTCT 
     1681 CAGACACGGC CAACTACACC TGTGTGGCCA AGAATATCGT GGCCAAGCGC CGGAGCACCA 
     1741 CGGCCACAGT CATCGTCTAT GTGAATGGAG GCTGGTCCAG CTGGGCAGAG TGGTCACCCT 
     1801 GTTCCAATCG CTGTGGCCGA GGCTGGCAGA AGCGTACTCG GACCTGCACC AATCCAGCCC 
     1861 CACTCAATGG AGGCGCCTTC TGTGAGGGAC AGGCCTTCCA GAAGACAGCT TGCACCACCG 
     1921 TGTGCCCAGT GGATGGAGCG TGGACCGAGT GGAGCAAGTG GTCTGCCTGC AGCACAGAGT 
     1981 GTGCGCACTG GCGCAGCCGC GAGTGCATGG CACCGCCACC CCAGAACGGA GGCCGTGACT 
     2041 GCAGCGGGAC GCTACTTGAC TCCAAGAACT GCACTGATGG GCTGTGCGTG CTGAATCAGA 
     2101 GAACTCTAAA CGACCCTAAA AGCCACCCCC TGGAGACATC GGGAGATGTG GCACTGTACG 
     2161 CAGGCCTTGT GGTGGCCGTC TTTGTGGTGG TAGCGGTTCT CATGGCCGTG GGAGTGATCG 
     2221 TATACCGGAG AAACTGCCGG GACTTCGACA CGGACATCAC CGACTCCTCT GCGGCCCTCA 
     2281 CTGGTGGCTT CCACCCTGTC AACTTCAAGA CTGCAAGGCC CAACAACCCG CAGCTCCTGC 
     2341 ACCCGTCCGC CCCTCCAGAC CTAACGGCCA GTGCTGGCAT CTACCGCGGG CCTGTGTATG 
     2401 CCCTGCAGGA CTCCGCCGAC AAGATCCCCA TGACTAATTC GCCCCTGCTG GATCCCCTGC 
     2461 CCAGCCTCAA GATCAAGGTC TATAACTCCA GCACCATCGG TTCTGGGTCT GGCCTGGCTG 
     2521 ATGGAGCCGA CCTGCTGGGT GTCCTCCCGC CGGGCACGTA CCCAGGCGAT TTCTCCCGGG 
     2581 ACACCCATTT CCTGCACCTG CGCAGTGCCA GCCTTGGTTC CCAGCACCTC CTGGGCCTAC 
     2641 CTCGGGACCC CAGCAGCAGT GTCAGCGGCA CCTTTGGTTG CCTGGGAGGA AGGCTGAGCC 
     2701 TCCCCGGCAC AGGGGTCAGC CTGTTGGTAC CAAATGGAGC CATTCCCCAG GGCAAGTTCT 
     2761 ATGACCTGTA TCTACATATC AACAAGGCCG AAAGCACCCT CCCACTTTCA GAAGGTTCCC 
     2821 AGACAGTATT GAGCCCCTCG GTGACCTGTG GGCCCACAGG CCTACTCCTG TGCCGCCCTG 
     2881 TCGTCCTCAC CGTGCCCCAC TGTGCTGAAG TCATCGCTGG AGACTGGATC TTTCAGCTCA 
     2941 AGACCCAGGC CCATCAGGGC CACTGGGAGG AGGTGGTGAC CTTGGATGAG GAGACCCTCA 
     3001 ACACACCCTG CTACTGCCAG CTGGAGGCTA AGTCCTGCCA CATCCTGCTG GACCAGCTGG 
     3061 GTACCTACGT ATTCATGGGC GAGTCCTACT CTCGCTCTGC AGTCAAGCGG CTCCAGCTGG 
     3121 CCATCTTCGC CCCAGCCCTC TGCACCTCCC TGGAGTATAG CCTCAGGGTC TACTGTCTGG 
     3181 AGGACACACC TGTAGCACTG AAGGAGGTCC TGGAGCTGGA GAGGACTCTG GGTGGCTACT 
     3241 TGGTGGAGGA GCCCAAGCCT TTGCTCTTTA AGGACAGTTA CCACAACCTA CGCCTCTCCC 
     3301 TCCATGACAT CCCCCATGCC CACTGGAGGA GCAAACTACT GGCCAAGTAC CAGGAGATTC 
     3361 CCTTCTACCA CGTCTGGAAT GGCAGCCAGA GAGCCCTGCA CTGCACTTTC ACCCTGGAGA 
     3421 GGCATAGCCT GGCCTCCACG GAGTTCACCT GTAAGGTCTG CGTGCGGCAG GTCGAAGGGG 
     3481 AAGGCCAGAT TTTCCAGCTG CACACAACGT TGGCCGAGAC GCCTGCTGGC TCCCTGGATG 
     3541 CTCTCTGCTC TGCCCCGGGC AATGCCATCA CCACCCAGCT GGGACCCTAT GCCTTCAAGA 
     3601 TACCCCTGTC CATCCGCCAA AAGATCTGCA GCAGCCTGGA CGCCCCCAAC TCCCGGGGCA 
     3661 ACGACTGGAG GCTGTTGGCG CAGAAGCTGT CCATGGACCG GTACCTAAAC TACTTCGCCA 
     3721 CCAAAGCTAG TCCCACAGGT GTCATCTTAG ACCTCTGGGA AGCTCGGCAA CAGGATGACG 
     3781 GGGACCTCAA CAGCCTGGCC AGTGCCTTGG AGGAGATGGG CAAGAGTGAG ATGCTGGTAG 
     3841 CCATGGCCAC AGATGGCGAT TGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3901 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3961 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4021 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4081 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4141 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4201 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4261 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4321 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4381 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4441 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4501 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4561 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4621 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4681 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4741 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4801 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4861 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4921 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4981 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5041 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5101 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5161 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5221 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5281 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5341 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5401 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5461 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5521 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5581 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5641 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5701 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5761 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5821 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5881 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5941 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6001 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6061 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6121 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6181 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6241 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6301 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6361 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6421 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6481 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6541 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6601 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6661 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6721 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6781 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6841 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6901 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6961 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7081 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7141 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7201 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7261 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7321 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7381 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7441 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7501 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7561 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7621 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7681 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//