LOCUS       MR224289                8289 bp    DNA     circular  SYN 
DEFINITION  Myo1b (Myc-DDK-tagged) - Mouse myosin IB (Myo1b), transcript variant 1
ACCESSION   NM_001161817
VERSION     NM_001161817.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4446..4537 
                     /label="Myc-DDK"  
     misc_feature    4567..5152 
                     /label="PolyA signal"  
     misc_feature    4624..4643 
                     /label="Restriction Enzyme"  
     misc_feature    5428..6100 
                     /label="ColE1"  
     misc_feature    6530..7324 
                     /label="Kan/Neo"  
     misc_feature    7359..7661 
                     /label="SV40 ori"  
     misc_feature    7747..8224 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4428..4434 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4428 
                     /label="Myo1b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAAAATG GAGGTGAAAT CCTCACTTCT GGACAATATG ATCGGTGTTG 
     1081 GAGATATGGT TCTTTTAGAG CCGCTCAATG AGGAGACGTT CATCGACAAC CTGAAGAAGC 
     1141 GCTTCGACCA CAATGAGATA TATACATACA TTGGGAGCGT GGTTATATCT GTTAACCCCT 
     1201 ACCGGTCCTT GCCCATATAT TCGCCGGAGA AAGTGGAAGA TTACAGAAAT AGAAACTTCT 
     1261 ATGAACTGAG CCCTCACATC TTTGCTTTGT CGGATGAAGC ATACAGATCC CTCCGTGATC 
     1321 AAGATAAAGA TCAATGCATT CTCATTACTG GGGAGAGTGG AGCAGGAAAA ACAGAGGCCA 
     1381 GCAAGCTCGT CATGTCCTAT GTCGCTGCCG TGTGCGGAAA AGGAGCAGAA GTCAATCAGG 
     1441 TCAAGGAACA GCTTCTGCAG TCCAACCCTG TCCTGGAAGC TTTTGGAAAT GCCAAGACTG 
     1501 TGAGGAATGA CAACTCATCT AGATTCGGCA AATACATGGA TATTGAATTT GACTTTAAAG 
     1561 GCGATCCGCT GGGAGGAGTG ATAAGTAACT ATCTTTTAGA GAAATCTCGT GTTGTTAAGC 
     1621 AGCCAAGAGG TGAAAGAAAC TTCCATGTGT TCTATCAGCT GCTCTCAGGC GCCTCTGAGG 
     1681 AGCTCCTCTA CAAGCTTAAG CTGGAGCGGG ATTTCAGCAG ATACAACTAT CTGAGTCTGG 
     1741 ACTCTGCTAA AGTTAATGGT GTGGATGATG CTGCCAATTT CAGAACTGTT AGGAATGCGA 
     1801 TGCAGATCGT GGGCTTTCTG GATCATGAGG CCGAGGCTGT CCTGGAGGTA GTGGCAGCCG 
     1861 TTTTGAAACT GGGGAACATC GAGTTCAAGC CTGAATCACG AGTGAATGGT TTGGATGAAA 
     1921 GCAAAATCAA AGATAAAAAT GAGTTAAAAG AAATTTGCGA GTTGACCAGC ATCGATCAGG 
     1981 TGGTTTTAGA AAGAGCGTTC AGTTTCCGAA CAGTTGAAGC GAAGCAGGAG AAAGTTTCAA 
     2041 CCACGCTGAA TGTGGCTCAG GCTTATTATG CCCGTGATGC CCTGGCTAAA AACCTCTACA 
     2101 GCCGCTTGTT TTCATGGTTG GTAAATCGTA TCAACGAAAG CATTAAGGCA CAAACAAAAG 
     2161 TGAGAAAGAA GGTCATGGGT GTTCTGGACA TTTATGGCTT TGAAATATTT GAGGATAACA 
     2221 GCTTCGAGCA GTTTATTATT AACTATTGTA ATGAAAAGCT TCAACAAATC TTCATCGAAC 
     2281 TTACCCTCAA AGAAGAGCAA GAGGAATATA TTCGGGAGGA CATAGAATGG ACTCACATTG 
     2341 ACTACTTCAA CAATGCTATC ATTTGTGACC TAATAGAAAA TAACACAAAT GGAATCTTGG 
     2401 CCATGCTGGA TGAAGAGTGC CTGAGACCCG GCACTGTTAC AGATGAGACC TTCCTGGAAA 
     2461 AGCTGAACCA AGTCTGTGCC ACCCACCAGC ACTTTGAGAG CAGGATGAGC AAGTGTTCCC 
     2521 GCTTTCTCAA CGACACAACC CTGCCCCACA GCTGCTTCAG AATCCAGCAT TATGCTGGCA 
     2581 AGGTGCTCTA CCAGGTGGAA GGATTTGTTG ACAAGAACAA TGACCTTCTG TACCGGGACC 
     2641 TGTCTCAAGC CATGTGGAAG GCCGGCCACT CCCTCATCAA GTCTCTGTTT CCTGAAGGAA 
     2701 ATCCTGCAAA GGTCAACCTG AAGAGACCTC CCACCGCAGG CTCCCAGTTC AAGGCGTCTG 
     2761 TGGCCACGCT GATGAGAAAC CTGCAGACCA AGAACCCGAA CTACATCAGG TGCATCAAGC 
     2821 CAAATGATAA AAAGGCAGCT CACATTTTCA ATGAGAGTCT CGTATGCCAT CAGATCAGGT 
     2881 ACCTGGGTCT TTTGGAGAAC GTTCGAGTGA GACGGGCAGG CTATGCCTTC AGGCAGGCCT 
     2941 ATGAACCTTG CCTGGAAAGA TACAAGATGC TTTGCAAACA AACGTGGCCT CACTGGAAGG 
     3001 GACCAGCAAG GTCTGGCGTG GAGGTTCTGT TTAATGAGCT AGAGATTCCT GTGGAAGAGC 
     3061 ACTCCTTCGG GAGATCCAAG ATATTCATCC GAAACCCACG GACATTATTC CAACTAGAAG 
     3121 ACCTAAGGAA GCAGCGGCTG GAGGACCTGG CCACTCTCAT TCAGAAGATT TATCGAGGAT 
     3181 GGAAATGCCG TACACATTTT CTGCTCATGA AAAGAAGCCA AGTTGTGATT GCTGCCTGGT 
     3241 ACAGGAGATA TGCGCAACAA AAGCGGTACC AGCAGATAAA GAGTTCAGCC CTGGTGATTC 
     3301 AGTCGTACAT CCGGGGCTGG AAGGCTCGAA AAATCCTGCG GGAGCTGAAG CATCAGAAGC 
     3361 GCTGTAAGGA GGCAGCCACC ACCATCGCGG CGTACTGGCA TGGCACCCAG GCGCGAAGGG 
     3421 AGCTGAGACG GCTGAAGGAC GAGGCTAGGA ATAAGCACGC TATTGCAGTT ATTTGGGCTT 
     3481 ACTGGCTTGG ATCGAAGGCA CGGAGGGAGT TGAAACGCTT GAAGGAGGAG GCTAGGCGTA 
     3541 AGCATGCAGT TGCTGTCATT TGGGCTTACT GGCTTGGACT GAAGGTACGC AGGGAATACA 
     3601 GGAAATTCTT TAGAGCAAAT GCTGGAAAGA AAATCTATGA GTTTACACTT CAAAGAATTG 
     3661 TGCAAAAATA CTTGTTGGAA ATGAAAAATA AAATGCCTTC CTTGTCACCG ATAGATAAAA 
     3721 ATTGGCCATC AAGACCTTAC CTGTTCTTGG ATTCGACTCA CAAGGAGCTA AAAAGGATCT 
     3781 TCCACTTGTG GAGGTGTAAA AAATACAGGG ATCAATTCAC AGACCAGCAG AAACTTATTT 
     3841 ATGAAGAGAA GCTCGAGGCC AGCGAACTCT TCAAAGACAA GAAGGCTTTA TATCCTTCTA 
     3901 GTGTTGGGCA ACCATTCCAA GGAGCTTACC TGGAAATCAA CAAGAACCCA AAATATAAGA 
     3961 AACTCAAAGA TGCCATTGAA GAGAAGATCA TTATTGCTGA GGTTGTGAAC AAAATTAATC 
     4021 GGGCTAATGG GAAGAGTACA TCTCGGATTT TCCTCTTAAC AAACAATAAC CTTCTCCTTG 
     4081 CTGACCAAAA GTCTGGGCAG ATCAAGTCCG AGGTCCCCCT GGTAGATGTG ACCAAGGTTT 
     4141 CCATGAGTTC CCAGAACGAT GGTTTCTTTG CAGTGCACCT CAAGGAGGGT TCAGAAGCGG 
     4201 CTAGTAAAGG GGACTTTCTC TTCAGCAGTG ACCACCTGAT TGAAATGGCA ACCAAGCTGT 
     4261 ATCGCACGAC TCTCAGCCAA ACTAAACAGA AGCTCAACAT CGAGATTTCC GATGAGTTCC 
     4321 TGGTACAGTT CAGACAGGAC AAAGTATGTG TGAAGTTTAT TCAAGGCAAC CAGAAAAATG 
     4381 GGAGCGTGCC AACCTGCAAA CGAAAGAACA ACAGACTCCT TGAAGTCGCT GTCCCTACGC 
     4441 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4501 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4561 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4621 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4681 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4741 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4801 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4861 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4921 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4981 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5041 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5101 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5161 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5221 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5281 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5341 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5401 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5461 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5521 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5581 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5641 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5701 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5761 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5821 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5881 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5941 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6001 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6061 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6121 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6181 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6241 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6301 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6361 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6421 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6481 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6541 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6601 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6661 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6721 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6781 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6841 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6901 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6961 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7021 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7081 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7141 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7201 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7261 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7321 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7381 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7441 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7501 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7741 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7801 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7861 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7921 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7981 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8041 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8101 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8161 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8221 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8281 GCGAATTTT
//