LOCUS       MR224271                6939 bp    DNA     circular  SYN 
DEFINITION  Catsper1 (Myc-DDK-tagged) - Mouse cation channel, sperm associated 1 (Catsper1)
ACCESSION   NM_139301
VERSION     NM_139301.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3096..3187 
                     /label="Myc-DDK"  
     misc_feature    3217..3802 
                     /label="PolyA signal"  
     misc_feature    3274..3293 
                     /label="Restriction Enzyme"  
     misc_feature    4078..4750 
                     /label="ColE1"  
     misc_feature    5180..5974 
                     /label="Kan/Neo"  
     misc_feature    6009..6311 
                     /label="SV40 ori"  
     misc_feature    6397..6874 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3078..3084 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3078 
                     /label="Catsper1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCAATCT TCAAGGAGGG ACGAGTCTTA CCATGAGACA CACCCCGGTA 
     1081 GCTTAGACCC AAGCCATCAA TCCCACCCCC ACCCCCACCC CCACCCCACC CTGCATAGGC 
     1141 CAAACCAAGG TGGCGTCTAT TACGATTCTC CTCAGCATGG GATGTTCCAA CAACCATACC 
     1201 AACAGCATGG TGGGTTCCAT CAACAAAATG AATTGCAGCA TTTGCGTGAG TTCTCAGACT 
     1261 CCCATGACAA TGCTTTCTCC CATCATTCCT ATCAGCAGGA CAGGGCTGGT GTCTCTACCT 
     1321 TACCTAATAA CATCTCTCAC GCTTATGGTG GATCGCACCC CCTTGCTGAG TCCCAGCACT 
     1381 CTGGTGGACC CCAGAGTGGA CCTCGTATTG ACCCGAATCA TCATCCCCAC CAAGATGACC 
     1441 CCCATAGACC CAGTGAACCT TTGTCCCACC CCTCCTCTAC TGGCAGCCAC CAAGGGACAA 
     1501 CCCATCAACA ATACCATGAG AGGTCTCACC ATCTTAACCC CCAGCAGAAC AGAGATCATG 
     1561 CAGACACCAT TTCCTATCGC TCCAGTACGC GGTTCTATCG CTCCCATGCG CCGTTCAGTC 
     1621 GCCAGGAAAG ACCTCATCTC CACGCTGATC ACCATCATGA GGGCCATCAT GCCCATTCTC 
     1681 ACCATGGTGA ACATCCCCAC CACAAGGAGC AACGGCACTA TCACGGAGAT CATATGCATC 
     1741 ACCATATCCA TCATCGCTCC CCAAGTGCCT CCCAACTCTC ACACAAGTCT CACAGCACAC 
     1801 TTGCCACCTC TCCATCTCAT GTGGGATCGA AAAGCACAGC CAGTGGGGCT CGGTACACCT 
     1861 TTGGAGCTCG CTCTCAAATT TTCGGCAAAG CTCAGTCCAG GGAGAGCTTG AGAGAATCAG 
     1921 CCTCTTTGAG TGAAGGGGAG GATCACGTTC AGAAACGCAA AAAGGCCCAG AGGGCCCACA 
     1981 AGAAGGCACA CACCGGGAAT ATCTTCCAAT TGCTATGGGA AAAAATAAGC CATCTCCTTT 
     2041 TGGGTCTTCA GCAAATGATA TTGTCACTGA CCCAGTCCCT GGGCTTTGAG ACCTTTATCT 
     2101 TCATCGTGGT CTGCCTCAAC ACAGTCATCC TTGTGGCCCA GACTTTCACT GAGCTAGAGA 
     2161 TCCGAGGTGA ATGGTACTTC ATGGTCTTGG ACTCCATCTT CCTCTCCATC TATGTACTAG 
     2221 AAGCAGTCCT CAAGCTAATT GCCCTGGGCC TGGAGTATTT TTATGACCCA TGGAACAACC 
     2281 TGGACTTCTT CATCATGGTC ATGGCAGTGC TGGACTTTGT GCTCCTTCAG ATAAACTCGC 
     2341 TCTCATATTC ATTCTACAAC CACAGCCTGT TCCGGATTCT CAAAGTCTTC AAAAGTATGC 
     2401 GGGCCCTGAG GGCCATCCGG GTTCTTCGGA GGCTCAGCAT CCTGACCAGC CTCCACGAAG 
     2461 TGGCCGGGAC TCTGAGTGGA TCTTTACCAT CCATCACGGC CATCCTCACC CTCATGTTTA 
     2521 CCTGCCTCTT CCTCTTCTCT GTGGTTCTCC GAGCACTGTT TCAGGACTCA GACCCCAAGC 
     2581 GCTTCCAGAA CATCTTTACC ACACTCTTCA CCCTGTTCAC CATGCTCACC CTGGACGACT 
     2641 GGTCCCTCAT CTACATAGAC AACAGGGCCC AAGGCGCCTG GTACATCATA CCGATCCTCA 
     2701 TGATTTACAT TGTCATCCAG TACTTCATCT TCCTCAACCT GGTGATTGCT GTCCTGGTAG 
     2761 ATAACTTCCA GATGGCGTTG CTCAAAGGCC TAGAGAAAGT GAAGCTGGAG CAAGCTGCCC 
     2821 GGGTCCATGA GAAGTTGTTG GATGACTCTC TGACAGATCT CAACAAAGCA GACGCCAATG 
     2881 CACAAATGAC CGAGGAGGCC TTGAAGATGC AGCTTATTGA GGGAATGTTT GGCAACATGA 
     2941 CAGTGAAGCA GCGGGTGCTC CACTTCCAGT TCCTGCAGTT GGTGGCAGCG GTAGAACAGC 
     3001 ATCAACAGAA GTTTCGTTCC CAAGCGTACG TCATCGATGA GCTTGTGGAC ATGGCATTTG 
     3061 AGGCTGGAGA CGACGACTAC GGGAAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3121 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3181 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3241 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3301 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3361 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3421 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3481 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3541 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3601 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3661 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3721 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3781 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     3841 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     3901 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     3961 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4021 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4081 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4141 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4201 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4261 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4321 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4381 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4441 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4501 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4561 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4621 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4681 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4741 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4801 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     4861 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     4921 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     4981 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5041 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5101 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5161 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5221 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5281 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5341 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5401 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5461 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5521 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5581 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5641 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5701 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5761 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     5821 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     5881 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     5941 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6001 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6061 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6121 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6181 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6241 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6301 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6361 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6421 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6481 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6541 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6601 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6661 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6721 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6781 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     6841 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     6901 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//