LOCUS       MR224181                8325 bp    DNA     circular  SYN 
DEFINITION  Atp8a2 (Myc-DDK-tagged) - Mouse ATPase, aminophospholipid transporter-like, class I, type 8A, member 2 (Atp8a2)
ACCESSION   NM_015803
VERSION     NM_015803.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4482..4573 
                     /label="Myc-DDK"  
     misc_feature    4603..5188 
                     /label="PolyA signal"  
     misc_feature    4660..4679 
                     /label="Restriction Enzyme"  
     misc_feature    5464..6136 
                     /label="ColE1"  
     misc_feature    6566..7360 
                     /label="Kan/Neo"  
     misc_feature    7395..7697 
                     /label="SV40 ori"  
     misc_feature    7783..8260 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4464..4470 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4464 
                     /label="Atp8a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCGGGCC ACGTCTGTTG GAGACCAGCT AGAGGCACCG GCCCGCATAA 
     1081 TTTACCTCAA CCAATCTCAT CTCAACAAAT TCTGCGACAA CCGGATCAGT ACGGCCAAGT 
     1141 ACAGCGTGTT GACATTTCTA CCTCGATTCC TGTATGAGCA GATTAGGAGA GCTGCTAACG 
     1201 CCTTCTTCCT CTTCATTGCC TTATTACAGC AAATCCCAGA CGTCTCACCA ACAGGACGGT 
     1261 ACACCACCCT GGTGCCCTTG GTCATCATTC TAACGATTGC AGGCATCAAA GAGATCATAG 
     1321 AAGATTTTAA ACGACATAAG GCAGACAATG CAGTTAACAA GAAGAAAACA ATAGTGTTAA 
     1381 GAAATGGCAT GTGGCACACT ATCATGTGGA AAGAGGTGGC AGTGGGAGAC ATCGTGAAGG 
     1441 TCCTCAATGG GCAGTATCTT CCAGCAGACA TGGTCCTGTT CTCCTCCAGT GAGCCCCAGG 
     1501 GGATGTGCTA TGTTGAAACT GCTAACCTGG ATGGCGAGAC GAACCTTAAA ATACGACAGG 
     1561 GCTTGAGCCA CACAACCGAC ATGCAGACGA GGGACGTGCT CATGAAGCTG TCGGGAAGGA 
     1621 TAGAGTGTGA AGGGCCCAAC CGCCACCTCT ACGACTTCAC CGGCAACCTG CACTTGGATG 
     1681 GGAAGAGCTC CGTTGCCCTT GGACCTGACC AGATCCTTCT GAGAGGCACC CAGCTCAGGA 
     1741 ACACACAGTG GGTCTTCGGC GTCGTTGTGT ACACTGGACA TGACAGCAAG CTCATGCAGA 
     1801 ATTCAACAAA AGCACCTCTC AAGAGATCAA ATGTTGAGAA GGTAACCAAC GTGCAGATCC 
     1861 TGGTGCTGTT TGGTATCCTC CTGGTCATGG CGCTGGTGAG CTCTGTGGGG GCCCTGTTCT 
     1921 GGAATGGGTC TCATGGCGGA AAGAGTTGGT ACATCAAGAA GATGGACACG AACTCAGATA 
     1981 ATTTTGGCTA CAACTTGCTA ACGTTTATCA TCTTGTACAA CAATCTGATT CCTATCAGCC 
     2041 TGCTGGTGAC ACTTGAGGTT GTGAAATACA CGCAAGCCCT CTTCATAAAC TGGGACATGG 
     2101 ATATGTATTA TATTGAAAAT GACACTCCTG CAATGGCCAG GACATCAAAC CTCAACGAAG 
     2161 AGCTGGGGCA GGTAAAATAC CTGTTTTCAG ACAAGACTGG AACTCTTACA TGTAATATCA 
     2221 TGAACTTCAA GAAGTGTAGC ATTGCTGGTG TGACCTATGG CCACTTCCCG GAACTAGCCA 
     2281 GAGAGCAGTC CTCAGATGAC TTCTGCCGGA TGACTTCTTG TACCAATGAC TCATGTGACT 
     2341 TCAATGATCC CAGGCTGTTA AAGAACATCG AGGATCAGCA CCCTACAGCC CCTTGCATAC 
     2401 AAGAGTTCCT TACCCTGCTG GCTGTGTGTC ACACCGTCGT CCCCGAGAAG GATGGAGATG 
     2461 AAATTATCTA CCAAGCCTCG TCCCCAGATG AAGCTGCTTT GGTCAAAGGA GCTAAGAAGC 
     2521 TTGGCTTTGT GTTTACCGGG AGGACGCCGT ACTCGGTCAT CATCGAAGCG ATGGGACAAG 
     2581 AACAGACATT CGGGATCCTC AATGTTCTAG AATTTTCTAG TGACAGGAAA AGAATGTCTG 
     2641 TCATTGTCCG ACTGCCATCA GGACAACTTC GACTCTACTG CAAGGGAGCC GATAACGTCA 
     2701 TCTTTGAAAG ACTCTCAAAG GACTCAAAGT ACATGGAGGA GACGCTATGC CATCTGGAAT 
     2761 ATTTTGCCAC AGAAGGCCTG CGGACTCTGT GCGTGGCCTA CGCAGACCTT TCTGAGAATG 
     2821 AGTATGAGGA GTGGCTGAAA GTCTATCAAG AGGCCAGCAT CATTCTGAAG GACAGAGCCC 
     2881 AGAGGCTGGA AGAGTGTTAC GAGATCATTG AGAAGAATTT ACTGTTACTT GGAGCTACAG 
     2941 CCATCGAAGA CCGTCTTCAA GCCGGCGTTC CAGAAACCAT AGCCACTCTG CTGAAGGCAG 
     3001 AAATCAAAAT CTGGGTGTTG ACAGGAGACA AACAAGAAAC TGCAATTAAT ATCGGGTATT 
     3061 CCTGTCGGTT GGTGTCGCAG AACATGGCCC TTATCCTATT GAAGGAGGAC TCTTTAGATG 
     3121 CAACAAGGGC GGCCATCACT CAGCACTGCA CAGATCTTGG AAATTTGCTG GGCAAAGAGA 
     3181 ACGATGTGGC CCTCATCATC GATGGCCATA CCCTGAAGTA TGCACTCTCC TTTGAAGTCC 
     3241 GCAGAAGTTT CCTGGACCTG GCGCTCTCAT GCAAAGCTGT CATCTGCTGC AGAGTGTCCC 
     3301 CTCTGCAGAA GTCTGAGATC GTGGATGTTG TGAAGAAGCG AGTGAAAGCC ATCACCCTGG 
     3361 CCATCGGAGA CGGTGCCAAT GACGTGGGGA TGATCCAGAC AGCCCACGTA GGGGTCGGGA 
     3421 TCAGCGGGAA CGAGGGAATG CAGGCTACCA ACAACTCAGA TTATGCCATC GCACAGTTTT 
     3481 CCTACCTGGA GAAGCTGCTT TTGGTCCACG GAGCCTGGAG CTACAACCGG GTGACCAAGT 
     3541 GTATCCTGTA CTGTTTCTAC AAGAATGTGG TCCTCTACAT CATCGAGCTA TGGTTCGCCT 
     3601 TTGTGAATGG ATTTTCTGGG CAGATTTTAT TCGAGCGCTG GTGCATCGGC TTGTACAATG 
     3661 TGATCTTCAC GGCATTGCCG CCCTTCACTC TGGGGATCTT CGAGAGGTCT TGTACTCAGG 
     3721 AGAGCATGCT CAGGTTCCCA CAGCTTTACA GAATCACTCA GAACGCTGAA GGTTTCAACA 
     3781 CTAAGGTTTT CTGGGGTCAC TGCATCAATG CCTTGGTTCA TTCCCTCATC CTCTTCTGGG 
     3841 TTCCCATGAA AGCGCTGGAG CATGATACTC CAGTAACCAG CGGTCATGCC ACAGACTATT 
     3901 TGTTTGTTGG AAATATTGTT TACACGTACG TTGTGGTTAC AGTTTGTTTG AAAGCTGGTT 
     3961 TGGAGACGAC AGCTTGGACG AAATTCAGTC ACCTGGCGGT GTGGGGAAGC ATGCTGATCT 
     4021 GGTTGGTGTT CTTCGGTGTC TATTCAACCA TCTGGCCGAC CATCCCCATT GCTCCTGACA 
     4081 TGAAAGGGCA GGCAACTATG GTCCTGAGCT CTGCGTACTT CTGGTTGGGA TTGTTCCTGG 
     4141 TTCCGACTGC GTGTTTGATT GAAGACGTGG CGTGGAGAGC GGCCAAGCAC ACCTGCAAAA 
     4201 AGACTCTGCT GGAGGAGGTG CAGGAGCTGG AGACCAAGTC CCGAGTGATG GGCAAGGCGA 
     4261 TGCTTCGAGA CAGTAACGGA AAGAGGATGA ACGAGCGTGA CCGTCTGATC AAGAGGCTCA 
     4321 GCAGGAAGAC ACCTCCAACC CTCTTCCGAA CAGGCTCCAT CCAGCAGTGT GTCAGCCATG 
     4381 GGTATGCCTT TTCTCAAGAA GAGCATGGAG CTGTTACACA GGAGGAAATA GTCCGCGCTT 
     4441 ATGATACCAC CAAAGAGAAT TCAAGGAAGA AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4501 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4561 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4621 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4681 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4741 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4801 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4861 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4921 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4981 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5041 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5101 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5161 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5221 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5281 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5341 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5401 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5461 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5521 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5581 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5641 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5701 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5761 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5821 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5881 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5941 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6001 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6061 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6121 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6181 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6241 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6301 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6361 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6421 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6481 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6541 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6601 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6661 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6721 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6781 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6841 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6901 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6961 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7021 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7081 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7141 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7201 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7261 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7321 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7381 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7441 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7501 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7561 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7741 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7801 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7861 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7921 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7981 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8041 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8101 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8161 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8221 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8281 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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