LOCUS       MR224135                8319 bp    DNA     circular  SYN 
DEFINITION  Ccar1 (Myc-DDK-tagged) - Mouse cell division cycle and apoptosis regulator 1 (Ccar1)
ACCESSION   NM_026201
VERSION     NM_026201.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4476..4567 
                     /label="Myc-DDK"  
     misc_feature    4597..5182 
                     /label="PolyA signal"  
     misc_feature    4654..4673 
                     /label="Restriction Enzyme"  
     misc_feature    5458..6130 
                     /label="ColE1"  
     misc_feature    6560..7354 
                     /label="Kan/Neo"  
     misc_feature    7389..7691 
                     /label="SV40 ori"  
     misc_feature    7777..8254 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4458..4464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4458 
                     /label="Ccar1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCAGTTT GGAGGACAGA AGAATCCACC ATGGGCTACT CAGTTTACAG 
     1081 CCACTGCGGT CTCACAACCA GCTGCACTAG GTGTTCAGCA GCCATCACTT CTGGGAGCAT 
     1141 CTCCTACCAT TTATACCCAG CAGACTGCAT TGGCGGCGGC AGGCCTTACC ACACAAACGC 
     1201 CAGCAAACTA TCAGTTAACA CAAACTGCGG CACTGCAGCA ACAAGCTGCA GCTGTATTAC 
     1261 AGCAGCAATA TTCACAACCT CAGCAGGCCT TGTATAGTGT GCAGCAGCAG TTGCAACAAC 
     1321 CTCAGCAGAC CATTTTAACA CAGCCAGCTG TTGCATTGCC CACAAGCCTT AGCCTGTCGA 
     1381 CTCCTCAGCC TGCAGCACAG ATTACTGTAT CATATCCAAC ACCAAGGTCC AGTCAACAGC 
     1441 AAACTCAACC TCAGAAGCAG CGTGTTTTCA CAGGAGTGGT TACAAAGCTA CATGATACAT 
     1501 TTGGATTTGT GGATGAAGAT GTATTCTTTC AGCTTGGTGC TGTTAAAGGG AAAACCCCCC 
     1561 AAGTTGGTGA TAGAGTATTG GTTGAAGCAA CTTATAATCC TAATATGCCT TTTAAATGGA 
     1621 ATGCACAAAG AATTCAAACA CTACCAAATC AGAATCAGTC TCAAACGCAA CCTTTACTGA 
     1681 AGACTCCGAC TGCTGTTATT CAGCCGATTG TGCCACAGAC AACGTTTGGT GTTCAGGCAC 
     1741 AGCCCCAACC CCAGTCATTA TTGCAGGCCC AGATCTCAGC TGCCTCTATT ACACCACTAT 
     1801 TGCAGACGCA GCCACAGCCC TTATTACAGC AGCCACAGCA GAAAGCTGGT TTATTGCAGC 
     1861 CTCCTGTCCG AATAGTGTCA CAGCCACAAC CTGCGCGGAG ATTAGATCCA CCATCACGAT 
     1921 TTTCAGGAAG AAACGACAGA GGGGATCAAG TACCTAATAG AAAAGATGAC CGAAGTCGTG 
     1981 AAAGGGACAG AGAAAGACGC AGATCTAGAG AAAGATCACC TCAGAGGAAA CGTTCCCGGG 
     2041 AGAGGTCACC CCGGAGAGAA AGAGAGCGCT CCCCTCGGAG AGTCCGTCGT GTCGTTCCAC 
     2101 GGTACACAGT GCAGTTTTCA AAGTTTTCTT TAGATTGTCC CAGTTGTGAC ATGATGGAAC 
     2161 TAAGGCGCCG TTATCAGAAC TTATATATTC CTAGTGACTT TTTTGATGCT CAGTTTACAT 
     2221 GGGTGGATGC TTTCCCTTTG TCAAGACCAT TTCAACTGGG AAATTACTGC AATTTTTATG 
     2281 TGATGCACCG AGAAGTAGAG TCCTTAGAAA AAAATATGGC TGTTCTTGAT CCACCTGATG 
     2341 CTGACCACCT GTACAGTGCA AAGGTAATGC TGATGGCTAG CCCTAGTATG GAAGACTTGT 
     2401 ATCATAAGTC ATGTGCTCTT GCTGAAGACC CACAAGACCT TCGTGATGGT TTTCAGCATC 
     2461 CTGCTAGACT TGTTAAGTTT CTAGTGGGAA TGAAAGGCAA GGATGAAGCC ATGGCCATTG 
     2521 GAGGCCACTG GTCTCCTTCG CTGGATGGAC CAAACCCAGA AAAAGATCCC TCTGTGTTGA 
     2581 TTAAAACTGC CATTCGTTGT TGTAAGGCTC TGACAGGCAT TGATCTAAGT GTATGCACAC 
     2641 AGTGGTACCG TTTTGCAGAG ATTCGCTACC ATCGCCCTGA GGAGACCCAC AAGGGGCGTA 
     2701 CAGTTCCAGC TCATGTGGAG ACAGTGGTTT TATTTTTCCC GGATGTTTGG CATTGCCTTC 
     2761 CCACCCGCTC AGAGTGGGAA ACCCTCTCCC GAGGATACAA GCAGCAGCTG GTCGAGAAGC 
     2821 TTCAGGGTGA ACGCAAGAAG GCTGATGGAG AACAGGATGA AGAAGAGAAG GATGATGGTG 
     2881 AAGTTAAAGA GATCGCCACT CCTACCCATT GGTCTAAGCT TGATCCAAAG GCAATGAAGG 
     2941 TAAATGATCT CCGAAAAGAA TTAGAAAGTC GAGCTCTCAG TTCCAAAGGA CTAAAATCGC 
     3001 AGTTAATAGC TCGCCTAACA AAGCAGCTTA AAATAGAAGA ACAAAAAGAA GAGCAGAAGG 
     3061 AATTAGAGAA GTCTGAAAAG GAAGAGGAAG ATGAGGATGA TAAGAAGTCT GAGGATGATA 
     3121 AAGAGGAAGA AGAAAGAAAA CGTCAAGAAG AAGTGGAACG ACAGCGTCAA GAAAGAAGAT 
     3181 ACATTTTGCC TGATGAACCT GCCATAATTG TGCATCCGAA CTGGGCTGCA AAAAGTGGCA 
     3241 AGTTTGATTG CAGCATCATG TCTTTGAGTG TCCTTTTGGA TTACAGATTG GAAGATAATA 
     3301 AAGAACATTC TTTTGAGGTT TCACTGTTTG CAGAACTTTT CAATGAAATG CTTCAAAGAG 
     3361 ACTTTGGGGT TAGAATATAC AAATCATTAC TCTCTCTTCC TGAGAAAGAG GACAAAAAAG 
     3421 ATAAGGAGAA GAAAAGCAAA AAAGAAGAGA GAAAAGATAA AAAAGAAGAA AGAGAAGATG 
     3481 ATATTGATGA ACCAAAACCA AAACGGAGAA AATCAGGCGA CGATAAAGAC AAAAAAGAAG 
     3541 ACAGAGATGA GAGAAAGAAA GAAGAAAAAA GAAAAGATGA TTCTAAAGAT GATGATGAAA 
     3601 CTGAAGAAGA TAACAATCAA GATGAGTATG ACCCAATGGA GGCAGAGGAA GCTGAGGATG 
     3661 AAGATGACGA TAGGGAGGAG GAGGAAGTAA AACGAGATGA CAAAAGGGAT GTCAGCCGGT 
     3721 ACTGCAAGGA CAGACCTGCG AAAGATAAGG AAAAAGAGAA GCCTCAAATG GTCACAGTTA 
     3781 ACAGGGATCT GCTAATGGCC TTTGTTTATT TTGATCAAAG TCATTGCGGT TACCTTCTTG 
     3841 AAAAGGATTT GGAAGAAATA CTATATACTC TTGGACTGCA TCTTTCTCGG GCTCAGGTAA 
     3901 AGAAACTTCT TAATAAAGTA GTACTCCGAG AATCGTGCTT TTATCGGAAA TTAACAGACA 
     3961 CCTCGAAAGA TGATGAGAAC CATGAAGAGT CAGAGGCACT GCAGGAAGAC ATGCTAGGAA 
     4021 ACAGATTATT ACTTCCAACA CCAACAATAA AACAGGAATC AAAAGATGGA GAGGAAAATG 
     4081 TAGGGCTTAT TGTGTACAAT GGTGCAATGG TGGATGTTGG GAGTCTCCTA CAAAAACTGG 
     4141 AAAAGAGTGA GAAAGTAAGA GCTGAGGTGG AACAGAAGCT CCAGTTACTA GAGGAGAAAA 
     4201 CAGATGAAGA TGGGAAAACT ATATTAAACT TGGAGAACTC TAACAAAAGC CTCTCTGGTG 
     4261 AACTTAGAGA GGTCAAAAAA GACCTTGGTC AATTACAAGA AAACCTGGAG GTTTCAGAAA 
     4321 ACATGAATTT GCAATTTGAA AACCAATTGA ATAAAACACT CAGAAACTTA TCTACAGTTA 
     4381 TGGATGATAT CCACACTGTC CTCAAAAAGG ATAATGTAAA GAGTGAAGAC AGAGATGAGA 
     4441 AATCCAAGGA GAACGGCTCA GGTGTAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4501 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4561 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4621 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4681 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4741 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4801 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4861 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4921 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4981 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5041 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5101 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5161 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5221 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5281 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5341 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5401 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5461 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5521 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5581 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5641 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5701 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5761 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5821 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5881 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5941 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6001 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6061 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6121 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6241 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6301 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6361 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6421 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6481 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6541 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6601 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6661 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6721 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6781 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6841 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6901 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6961 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7021 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7081 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7141 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7201 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7261 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7321 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7381 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7441 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7501 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7561 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7741 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7801 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7861 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7921 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7981 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8041 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8101 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8161 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8221 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8281 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//