LOCUS       MR224118                7674 bp    DNA     circular  SYN 
DEFINITION  Sema6c (Myc-DDK-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C (Sema6c)
ACCESSION   NM_011351
VERSION     NM_011351.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3831..3922 
                     /label="Myc-DDK"  
     misc_feature    3952..4537 
                     /label="PolyA signal"  
     misc_feature    4009..4028 
                     /label="Restriction Enzyme"  
     misc_feature    4813..5485 
                     /label="ColE1"  
     misc_feature    5915..6709 
                     /label="Kan/Neo"  
     misc_feature    6744..7046 
                     /label="SV40 ori"  
     misc_feature    7132..7609 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3813..3819 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3813 
                     /label="Sema6c"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGTGCC CCCCACTCCA TGCCCCTGCT GCTCCTGCTG CTGTTGCTGT 
     1081 CATCGCTCCC CCAAGCCCAG GCTGCCTTTC CCCAGGACCC CACCCCTCTG TTGACCTCTG 
     1141 ACCTTCAAGG TGCCTCTCCA TCATCCTGGT TCCGGGGCCT GGAGGACGAT GCTGTGGCTG 
     1201 CGGAACTTGG GCTGGACTTT CAGAGATTCC TGACCTTGAA CCGGACCTTG CTTGTGGCTG 
     1261 CCCGGGATCA CGTTTTCTCC TTCGATCTTC AAGCCCAAGA AGAAGGGGAG GGGCTGGTGC 
     1321 CCAACAAGTT TCTGACATGG AGGAGTCAAG ATATGGAGAA CTGTGCAGTC CGGGGAAAGC 
     1381 TGACGGACGA ATGCTACAAC TACATCCGTG TTCTTGTTCC CTGGAACTCG CAGACACTTC 
     1441 TTGCCTGTGG AACAAATTCC TTCAGCCCTA TGTGCCGCAG CTATGGGATA ACATCTCTGC 
     1501 AACAGGAGGG TGAGGAGCTG AGTGGGCAGG CTCGATGCCC CTTTGATGCC ACCCAGTCCA 
     1561 CTGTGGCCAT CTTTGCAGAG GGCAGTTTGT ACTCAGCCAC AGCAGCAGAT TTCCAGGCCA 
     1621 GTGATGCTGT AGTTTACAGA AGTCTTGGAC CTCAGCCTCC ACTCCGTTCT GCAAAGTATG 
     1681 ACTCCAAGTG GCTTCGAGAG CCACACTTTG TCTATGCGTT GGAGCATGGA GAGCACGTCT 
     1741 ACTTCTTCTT CCGAGAAGTC TCTGTGGAGG ATGCCCGCTT AGGGAGGGTG CAGTTTTCCA 
     1801 GGGTAGCCCG GGTATGTAAA CGTGACATGG GTGGCTCACC TCGGGCCTTG GATCGCCACT 
     1861 GGACATCCTT CCTTAAGCTG AGGCTCAACT GCTCTGTCCC TGGGGACTCT ACCTTCTACT 
     1921 TTGACGTCTT ACAGTCCTTA ACTGGGCCTG TGAACCTGCA TGGCCGCTCC GCTCTCTTTG 
     1981 GGGTCTTCAC TACTCAGACC AATAGCATTC CTGGGTCTGC AGTCTGCGCC TTCTACCTAG 
     2041 ATGATATTGA ACGTGGCTTT GAGGGCAAGT TCAAGGAGCA GAGGAGTCTG GATGGGGCCT 
     2101 GGACTCCTGT GTCTGAGGAC AAGGTCCCCT CACCCAGGCC AGGGTCCTGT GCAGGTGTGG 
     2161 GTGCAGCTGC CTCGTTCTCC TCCTCTCAAG ACCTACCTGA TGATGTCCTG CTCTTCATCA 
     2221 AGGCACACCC GCTGCTGGAT CCTGCTGTGC CACCCGCCAC CCATCAACCG CTCCTCACTC 
     2281 TGACTAGCAG GGCCCTGCTG ACCCAGGTAG CTGTGGACGG AATGGCTGGT CCCCACAGAA 
     2341 ATACTACTGT CCTGTTTCTT GGCTCCAACG ATGGGACAGT GCTGAAGGTG CTACCTCCAG 
     2401 GGGGTCAGTC TTTGGGCTCT GAGCCTATCG TCTTGGAAGA GATTGATGCC TACAGCCATG 
     2461 CCCGGTGCAG TGGGAAGCGG TCACCCCGAG CTGCGCGGCG GATCATAGGG CTGGAGCTGG 
     2521 ACACTGAGGG TCACAGGCTT TTTGTGGCCT TTCCTGGATG TATCGTCTAC CTCTCTCTCA 
     2581 GCCGGTGTGC CCGGCATGGA GCATGTCAGA GGAGCTGCCT GGCTTCTCTG GACCCATACT 
     2641 GTGGATGGCA TCGATCCAGA GGCTGCATGA GTATCAGGGG ACCTGGTGGG ACTGATGTCG 
     2701 ATCTGACTGG GAACCAGGAA TCCACAGAGC ACGGTGACTG CCAAGATGGA GCGACCGGGA 
     2761 GTCAGTCTGG CCCTGGAGAT TCTGCTTATG GCGTGCGCAG GGACCTTTCC CCAGCCTCAG 
     2821 CCTCCCGATC CATTCCCATC CCACTCCTCC TGGCCTGTGT GGCCGCGGCC TTCGCTTTGG 
     2881 GCGCCTCAGT CTCCGGTCTC TTGGTGTCCT GTGCTTGTCG TCGCGCGAAC CGCCGTCGGA 
     2941 GCAAGGACAT CGAGACCCCG GGGCTGCCGC GCCCCCTCTC CCTTCGCAGC CTGGCCCGGC 
     3001 TGCACGGTGG CGGTCCTGAG CCCCCGCCTC CGCCCAAGGA TGGAGATGCA GCGCAAACGC 
     3061 CCCAGCTCTA CACTACCTTC CTGCCTCCGC CCGACGGCGG ATCCCCACCG GAGCTGGCCT 
     3121 GCCTACCCAC GCCGGAGACG ACGCCCGAGC TGCCCGTGAA GCACCTCCGT GCCTCCGGGG 
     3181 GCCCCTGGGA ATGGAACCAG AATGGGAACA ACGCCTCGGA GGGCCCAGGC CGCCCACCAC 
     3241 GGGGCTGCAG CGGGGCGGGC GGGCCCGCAC CGCGAGTGCT GGTGAGGCCA CCGCCCCCTG 
     3301 GCTGCCCCGG GCAGGCGGTA GAGGTGACCA CGCTGGAGGA ACTGCTGCGC TACCTGCACG 
     3361 GCCCTCAGCC GCCCAGGAAG GGTAGCGAAC CTCTCGCCTC CGCCCCGTTC ACCTCCCGGC 
     3421 CGCCCGCCTC GGAGCCCGGC GCCTCGCTGT TCGTGGACTC CAGCCCGATG CCGCGGGATG 
     3481 GCGTTCCGCC GCTCAGGCTC GACGTGCCAC CCGAAGGCAA GCGCGCTGCC CCGAGCGGGC 
     3541 GGCCTGCTCT CTCGGCCCCA GCCCCGCGCC TGGGTGTCGG CGGCAGCCGC CGATTGCCCT 
     3601 TTCCCACACA CCGGGCGCCC CCGGGCCTGC TCACGCGAGT GCCCTCGGGA GGCCCGGCCA 
     3661 GGTACTCCGG GGGGCCCGGG AGGCACCTCC TGTACCTGGG CCGGCCCGAA GGCCATCGCG 
     3721 GCCGCTCCCT GAAGAGGGTG GACGTGAAGT CTCCGCTGTC GCCCAAGCCG CCCCTCGCCT 
     3781 CCCCGCCGCA GCCCGCCCCG CACGGTGGTC ATTTCAACTT CACGCGTACG CGGCCGCTCG 
     3841 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3901 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3961 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4021 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4081 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4141 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4201 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4261 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4321 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4381 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4441 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4501 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4561 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4621 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4681 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4741 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4801 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4861 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4921 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4981 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5041 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5101 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5161 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5221 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5281 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5341 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5401 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5461 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5521 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5581 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5641 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5701 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5761 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5821 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5881 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5941 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6001 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6061 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6121 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6181 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6241 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6301 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6361 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6421 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6481 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6541 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6601 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6661 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6721 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6781 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6841 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6901 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6961 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7021 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7081 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7141 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7201 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7261 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7321 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7381 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7441 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7501 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7561 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7621 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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