LOCUS       MR224108                8097 bp    DNA     circular  SYN 
DEFINITION  Gucy2c (Myc-DDK-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 1
ACCESSION   NM_001127318
VERSION     NM_001127318.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4254..4345 
                     /label="Myc-DDK"  
     misc_feature    4375..4960 
                     /label="PolyA signal"  
     misc_feature    4432..4451 
                     /label="Restriction Enzyme"  
     misc_feature    5236..5908 
                     /label="ColE1"  
     misc_feature    6338..7132 
                     /label="Kan/Neo"  
     misc_feature    7167..7469 
                     /label="SV40 ori"  
     misc_feature    7555..8032 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4236..4242 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4236 
                     /label="Gucy2c"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGTCACTG CTGGGCTTGG CTGTGCGGTT ACTGCTCTTC CAGCCCGCGC 
     1081 TGATGGTGTT CTGGGCCTCT CAGGTGAGGC AGAACTGCCG CAATGGCAGC TACGAGATCA 
     1141 GCGTCCTGAT GATGGACAAC TCAGCCTACA AAGAACCTAT GCAAAACCTG AGGGAGGCTG 
     1201 TGGAGGAAGG ACTGGACATA GTGCGAAAGC GCCTGCGTGA AGCCGACCTA AATGTGACTG 
     1261 TGAACGCGAC TTTCATCTAC TCCGACGGTC TGATTCATAA GTCAGGTGAC TGCCGGAGCA 
     1321 GCACCTGTGA AGGCCTTGAC CTACTCAGGG AGATTACAAG AGATCATAAG ATGGGCTGCG 
     1381 CCCTCATGGG GCCCTCGTGC ACGTATTCCA CCTTCCAGAT GTACCTCGAC ACAGAGTTGA 
     1441 ACTATCCCAT GATTTCCGCT GGAAGTTATG GATTGTCCTG TGACTATAAG GAAACCCTAA 
     1501 CCAGGATCCT GCCTCCAGCC AGGAAGCTGA TGTACTTCTT GGTCGATTTC TGGAAAGTCA 
     1561 ACAATGCATC TTTCAAACCC TTTTCCTGGA ACTCTTCGTA TGTTTACAAG AATGGATCGG 
     1621 AACCTGAAGA TTGTTTCTGG TACCTCAATG CTCTGGAGGC TGGGGTGTCC TATTTTTCTG 
     1681 AGGTGCTCAA CTTCAAGGAT GTACTGAGAC GCAGCGAACA GTTCCAGGAA ATCTTAACAG 
     1741 GCCATAACAG AAAGAGCAAT GTGATTGTTA TGTGTGGCAC GCCAGAAAGC TTCTATGATG 
     1801 TGAAAGGTGA CCTCCAAGTG GCTGAAGATA CTGTTGTCAT CCTGGTAGAT CTGTTCAGTA 
     1861 ACCATTACTT TGAGGAGAAC ACCACAGCTC CTGAGTATAT GGACAATGTC CTCGTCCTGA 
     1921 CGCTGCCGTC TGAACAGTCC ACCTCAAACA CCTCTGTCGC CGAGAGGTTT TCATCGGGGA 
     1981 GAAGTGACTT TTCTCTCGCT TACTTGGAGG GAACCTTGCT ATTTGGACAC ATGCTGCAGA 
     2041 CGTTTCTTGA AAATGGAGAA AATGTCACGG GTCCCAAGTT TGCTCGTGCA TTCAGGAATC 
     2101 TCACTTTTCA AGGCTTTGCA GGACCTGTGA CTCTGGATGA CAGTGGGGAC ATTGACAACA 
     2161 TTATGTCCCT TCTGTATGTG TCTCTGGATA CCAGGAAATA CAAGGTTCTT ATGAAGTATG 
     2221 ACACCCACAA AAACAAAACT ATTCCGGTGG CTGAGAACCC CAACTTCATC TGGAAGAACC 
     2281 ACAAGCTCCC CAATGACGTT CCTGGGCTGG GCCCTCAAAT CCTGATGATT GCCGTCTTCA 
     2341 CGCTCACGGG GATCCTGGTA GTTCTGCTGC TGATTGCCCT CCTCGTGCTG AGAAAATACA 
     2401 GAAGAGATCA TGCACTTCGA CAGAAGAAAT GGTCCCACAT TCCTTCTGAA AACATCTTTC 
     2461 CTCTGGAGAC CAACGAGACC AACCACATCA GCCTGAAGAT TGACGATGAC AGGAGACGAG 
     2521 ACACAATCCA GAGAGTGCGA CAGTGCAAAT ACGACAAGAA GAAAGTGATT CTGAAAGACC 
     2581 TCAAGCACAG CGACGGGAAC TTCAGTGAGA AGCAGAAGAT AGACCTGAAC AAGTTGCTGC 
     2641 AGTCTGACTA CTACAACCTG ACTAAGTTCT ACGGCACCGT GAAGCTGGAC ACCAGGATCT 
     2701 TTGGGGTGGT TGAGTACTGC GAGAGGGGAT CCCTCCGGGA AGTGTTAAAC GACACAATTT 
     2761 CCTACCCTGA CGGCACGTTC ATGGATTGGG AGTTTAAGAT CTCTGTCTTA AATGACATCG 
     2821 CTAAGGGGAT GTCCTACCTG CACTCCAGTA AGATTGAAGT CCACGGGCGT CTCAAATCCA 
     2881 CCAACTGCGT GGTGGACAGC CGCATGGTGG TGAAGATCAC CGACTTTGGG TGCAATTCCA 
     2941 TCCTGCCTCC AAAAAAAGAC CTGTGGACGG CCCCGGAGCA CCTGCGCCAG GCCACCATCT 
     3001 CTCAGAAAGG AGACGTGTAC AGCTTCGCCA TCATTGCCCA GGAGATCATC CTCCGTAAGG 
     3061 AGACTTTTTA CACGCTGAGC TGTCGGGATC ACAATGAGAA GATTTTCAGA GTGGAAAATT 
     3121 CATACGGGAA ACCTTTCCGC CCAGACCTCT TCCTGGAGAC TGCAGATGAG AAGGAGCTGG 
     3181 AGGTCTATCT ACTTGTCAAA AGCTGTTGGG AGGAGGATCC AGAAAAGAGG CCAGATTTCA 
     3241 AGAAAATCGA GAGCACACTG GCCAAGATAT TTGGCCTTTT CCATGACCAG AAAAACGAGT 
     3301 CTTACATGGA CACCTTGATC CGACGTCTCC AGCTGTACTC TCGAAACCTG GAACATCTGG 
     3361 TGGAGGAAAG GACTCAGCTG TACAAGGCGG AGAGGGACAG GGCTGACCAC CTTAACTTCA 
     3421 TGCTCCTCCC ACGGCTGGTG GTAAAGTCAC TGAAGGAGAA AGGCATCGTG GAGCCAGAGC 
     3481 TGTACGAAGA AGTCACAATC TACTTCAGTG ACATTGTGGG CTTCACCACC ATCTGCAAGT 
     3541 ATAGCACGCC CATGGAGGTG GTGGACATGC TCAACGACAT CTACAAGAGC TTTGACCAGA 
     3601 TTGTGGACCA CCATGACGTC TACAAGGTAG AAACCATCGG TGACGCCTAC GTGGTGGCCA 
     3661 GCGGTCTGCC TATGAGAAAC GGCAACCGAC ACGCGGTAGA CATTTCCAAG ATGGCCTTGG 
     3721 ACATCCTCAG CTTCATAGGG ACCTTTGAGT TGGAGCATCT CCCTGGCCTC CCCGTGTGGA 
     3781 TCCGCATTGG AGTTCATTCT GGGCCCTGCG CTGCTGGTGT TGTGGGGATC AAGATGCCTC 
     3841 GCTATTGCCT GTTTGGAGAC ACTGTCAACA CTGCCTCCAG GATGGAATCC ACCGGCCTCC 
     3901 CCTTGAGGAT TCACATGAGC AGCTCCACCA TAACCATCCT GAAGAGAACG GATTGCCAGT 
     3961 TCCTGTATGA AGTGAGGGGA GAAACCTACT TAAAGGGAAG AGGGACAGAG ACCACATACT 
     4021 GGCTGACTGG GATGAAGGAC CAAGAATACA ACCTGCCATC CCCACCGACA GTGGAGAACC 
     4081 AACAGCGTCT GCAGACTGAG TTCTCAGACA TGATCGTTAG CGCCTTACAG AAAAGACAGG 
     4141 CCTCGGGCAA GAAGAGCCGG AGGCCCACTC GGGTGGCCAG CTACAAGAAA GGCTTTCTGG 
     4201 AATACATGCA GCTGAACAAT TCAGACCACG ATAGCACCTA TTTTACGCGT ACGCGGCCGC 
     4261 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4321 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4381 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4441 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4501 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4561 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4621 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4681 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4741 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4801 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4861 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4921 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4981 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5041 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5101 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5161 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5221 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5281 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5341 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5401 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5461 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5521 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5581 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5641 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5701 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5761 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5821 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5881 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5941 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6001 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6061 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6121 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6181 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6241 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6301 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6361 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6421 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6481 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6541 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6601 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6661 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6721 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6781 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6841 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6901 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6961 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7021 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7081 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7141 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7201 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7261 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7321 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7381 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7441 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7501 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7561 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7621 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7681 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7741 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7801 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7861 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7921 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7981 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8041 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//