LOCUS       MR224025                8724 bp    DNA     circular  SYN 
DEFINITION  Trps1 (Myc-DDK-tagged) - Mouse trichorhinophalangeal syndrome I (human) (Trps1)
ACCESSION   NM_032000
VERSION     NM_032000.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4881..4972 
                     /label="Myc-DDK"  
     misc_feature    5002..5587 
                     /label="PolyA signal"  
     misc_feature    5059..5078 
                     /label="Restriction Enzyme"  
     misc_feature    5863..6535 
                     /label="ColE1"  
     misc_feature    6965..7759 
                     /label="Kan/Neo"  
     misc_feature    7794..8096 
                     /label="SV40 ori"  
     misc_feature    8182..8659 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4863..4869 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4863 
                     /label="Trps1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCGGAAA AAGAACCCCC CTCTGAGAAA CGTTGCTAGT GAAGGCGAGG 
     1081 GTCAAACCCT GGAGCCAACA GCTACAGAGA GCAAGGTATC TGGGAAGAAC AAAGAATTGT 
     1141 CTGCAGATCA GATGTCAGAA AATACAGATC AGAGTGATGT TGCAGAGTTG AACTCGAAGG 
     1201 AGGAACACAG CACGCATGGC CAAGAGCCAT CTTCCAGCGG TAAGAAGGAC TTGCAAATCT 
     1261 CAGGCCTGAG TGAGAAGGCT GGCTTCAATT ATGAAAGCCC CAGTAAGGGA GGGAGCCTTG 
     1321 TCTCCTTCCC ACATGATGAG GTAACAGACA GAAATATGTT GGCTTTCTCA TCTCCAGCTG 
     1381 CTGGGGGAGT CTGCGAGCCC TTGAAGTCTC CTCAAAGAGC AGAGGCAGAT GACCCTCAAG 
     1441 ATATGGCCTG CACCCCATCA GGGGATTCAT TGGAGACAAA GGAAGAGCAT AAAATGTCAC 
     1501 CAAAGGCCAC TGAGGAAACA GGGCCAGTGC AGAGTGGTCA AGCCAATTGT CAAGGTTTGA 
     1561 GTCCAGTTTC CGTGGCCTCA AAAAACCCAC AAGTGCCTTC AGATGGGGGT GTCAGACTGA 
     1621 GTAAACCCAA AGGTGACTTA CTGGTGAATG ACAACCCAGA CCCGGCACCT CTGTCTCCAG 
     1681 AGCTTCAGGA CTTTAAATGC AATATCTGTG GATACGGTTA CTATGGCAAT GACCCCACAG 
     1741 ATCTGATTAA GCACTTCCGA AAGTATCACT TAGGACTGCA TAATCGCACC AGGCAGGATG 
     1801 CTGAGCTGGA CAGCAAAATC TTGGCCCTTC ATAACATGGT GCAGTTCAGC CATTCCAAAG 
     1861 ACTTCCAGAA GGTCAACCGT TCTGTGCTTT CTGGCGTGCT GCAGGATATC AGCTCTTCAC 
     1921 GGCCTGCTTT ACTAAACGGA ACCTATGATG TACAGGTCAC TTCAGGAGGA ACTTTCATTG 
     1981 GCATTGGACG GAAAACTCCA GATTGCCAAG GCAACACCAA GTATTTCCGC TGCAAATTCT 
     2041 GCAATTTCAC TTATATGGGA AACTCCTCAA CTGAACTAGA GCAACATTTT CTTCAGACGC 
     2101 ACCCGAATAA AATCAAAGTG TCTCTCCCGT CCTCCGAGGG TGTGAAACCT TCAGAGAAAA 
     2161 ACTCTAACAA ATCCATCCCT GCGCTTCGAG CCAGTGACTC TGGGGATGTG GGAAAGTGGC 
     2221 AGGACAAAAT GACAGTCAAA GCTGGAGATG ACACCCCTGT CGGCTACTCA GTGCCCATCA 
     2281 AGCCCCTCGA TTCCTCACGA CAAAATGGTA CAGAGGCCAC CAGTTATTAC TGGTGTAAAT 
     2341 TTTGTAGTTT CAGCTGTGAG TCATCCAGCT CCCTTAAGCT GCTAGAACAT TATGGCAAGC 
     2401 AGCACGGAGC AGTGCAGTCG GGCGGCCTTA ATCCAGAGTT GAATGACAAG CTTCCCAGGG 
     2461 GCTCTGTCAT TAATCAGAAT GATCTAGCCA AAAGTGTAGA AGGAGAGCCA TTGACCAAGC 
     2521 CAGAAAAGGG CTTGAGTGGG GCTAAAAAGA AGGACTTCCC CAGCAAGGGA GCAGAGGATA 
     2581 ATATGGTAAC GAGCTATAAC TGTCAGTTCT GTGACTTTAG ATATTCCAAA AGCCATGGCC 
     2641 CTGATGTGAT TGTAGTGGGT CCACTTCTCC GTCATTATCA GCAACTCCAT AACATCCACA 
     2701 AGTGTACCAT TAAGCACTGT CCATTCTGTC CCAGAGGCCT TTGCAGCCCA GAAAAGCACC 
     2761 TTGGAGAGAT TACTTATCCG TTTGCTTGTA GAAAAAGTAA TTGTTCCCAC TGTGCACTGT 
     2821 TGCTGTTGCA CTTGTCTCCT GGGGTGGCCG GAAGTTCGAG AGTCAAACAC CAATGCCACC 
     2881 AGTGTTCCTT CTCTACCCCC GATGTAGATG TGCTCCTCTT TCACTATGAG ACCGTGCATG 
     2941 AGTCCCAAGC ATCGGATGTC AAACAGGAGG CCAACCACCT GCTAGGATCT GACGGGCAGC 
     3001 AGGCTGTCAG GGACAGCAAG GAGCACTCGT GCACCAAATG CGATTTTATA ACCCAGGTGG 
     3061 AAGAAGAGAT CTCGAGACAC TACAGGAGAG CACACAGCTG CTATAAATGT CGTCAGTGCA 
     3121 GTTTCACAGC TGCTGACACT CAGTCACTAT TGGAGCACTT CAACACAGTT CACTGCCAGG 
     3181 AACAGGAAAT CACTACTGCT AATGGCGAAG AGGGTGGCCA TGCCATACCC ACCATCAAAG 
     3241 AAGAGCCCAA AATTGACCTC AAGGTCTACA GTCTGCTAAA CCCAGACTCT AAAATGGGAG 
     3301 AAACAGTTCC CGAGAGCATA GTCAAGAGAG AGAAACTTGA TGACAAGGAA GGGCTGAAAG 
     3361 ATAAAATCTG GACTGAGAGT TCAACTGATG ACCTTCGTGG TGTGGCTTGG AGAGGGGCAG 
     3421 ACATCCTACG GGGTAGTCCA TCCTACACTC AAGCAAGCTT AGGGGTCTTG ACGCCTGTGT 
     3481 CCAGCTCCCA AGAGCAGACA AAGACTCTGA GGGACAGCCC CAATGTTGAA GCTGCCCACC 
     3541 TGGCACGACC TATGTATGGC TTGGCAGTAG ATACCAAGGG CTTCCTGCAA GGGGCTCCTG 
     3601 CTGGCAGCGA GAAGTCTGCA TCTCTTACCC AGCAGTATCC TGCCTCCGGA GAGAGCAAGA 
     3661 CCAAGGATGA ATCCCAGTCG CTGTTACGGA GGCGAAGAGG ATCTGGTGTT TTTTGTGCCA 
     3721 ATTGCCTGAC CACAAAGACC TCTCTCTGGC GAAAGAATGC AAATGGCGGA TATGTATGCA 
     3781 ATGCATGTGG TCTCTACCAG AAACTTCACT CGACTCCCAG GCCTTTAAAC ATCATTAAAC 
     3841 AAAACAACGG CGAGCAGATT ATTAGAAGAC GGACAAGAAA GCGCCTTAAC CCAGAGGCAC 
     3901 TTCAGGCTGA GCAGCTCAAC AAACAGCAGA GGGGAAGTGG GGAGGAGCAG GTCAATGGAA 
     3961 GCCCCTTAGA GAGGAGGTCA GAAGATCACT TAAGTGAAAG TCATCCGAGA GAAATTCCAC 
     4021 TTCCGAGCCT GAGTAAATAC GAAGCCCAGG GTTCATTGAC TAAAAGCCAT TCTGCTCAGC 
     4081 AGCCAGTCCT GGTCAGCCAA GCTCTGGATA TCCACAAAAG GATGCAGCCT TTGCACATTC 
     4141 AGATAAAAAG TCCTCAGGAA AGTACTGGAG ATCCAGGAAA TAGTTCATCC GTATCTGATG 
     4201 GGAAAGGAAG TTCTGAAAGA GGCAGCCCCA TCGAAAAGTA CATGAGACCT GCAAAACACC 
     4261 CCAACTATTC TCCACCAGGC AGCCCCATCG AAAAGTACCA ATACCCGCTT TTTGGAGTTC 
     4321 CTTTTGTACA TAATGACTTC CAGAGTGAAG CTGATTGGCT ACGGTTCTGG AGTAAATATA 
     4381 AGCTCTCCGT TCCTGGGAAT CCGCACTACT TGAGTCATGT GCCTGGCTTA CCAAATCCTT 
     4441 GCCAAAACTA TGTGCCTTAT CCCACCTTCA ATCTGCCTCC TCATTTCTCA GCTGTTGGAT 
     4501 CAGACAATGA CATTCCTCTA GATTTGGCGA TCAAGCATTC CAGACCTGGG CCAACTGCAA 
     4561 ATGGTGCCTC CAAGGAGAAA ACCAAGGCAC CACCAACTGT AAAAAATGAA GGTCCCTTGA 
     4621 ATGTAGTTAA GACGGAGAAA GTTGACAGAA GCACTCAAGA TGAACTTTCA ACGAAATGTG 
     4681 TGCACTGTGG CATTGTTTTT CTGGATGAAG TGATGTATGC TTTGCATATG AGTTGCCATG 
     4741 GTGACAGTGG ACCTTTCCAG TGCAGCATAT GCCAGCATCT TTGCACGGAC AAATATGACT 
     4801 TCACAACACA TATCCAGAGG GGCCTGCATA GGAACAATGC ACAAGCAGAA AAAAATGGAA 
     4861 AACCTAAAGA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4921 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4981 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5041 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5101 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5161 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5221 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5281 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5341 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5401 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5461 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5521 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5581 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5641 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5701 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5761 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5821 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5881 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5941 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6001 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6061 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6121 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6181 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6241 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6301 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6361 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6421 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6481 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6541 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6601 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6661 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6721 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6781 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6841 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6901 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6961 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7021 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7081 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7141 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7201 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7261 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7321 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7381 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7441 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7501 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7561 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7621 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7681 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7741 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7801 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7861 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7921 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8161 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8221 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8281 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8341 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8401 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8461 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8521 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8581 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8641 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8701 TAACAAAAAT TTAACGCGAA TTTT
//