LOCUS       MR223994                8430 bp    DNA     circular  SYN 
DEFINITION  Dido1 (Myc-DDK-tagged) - Mouse death inducer-obliterator 1 (Dido1), transcript variant 2
ACCESSION   NM_177852
VERSION     NM_177852.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4587..4678 
                     /label="Myc-DDK"  
     misc_feature    4708..5293 
                     /label="PolyA signal"  
     misc_feature    4765..4784 
                     /label="Restriction Enzyme"  
     misc_feature    5569..6241 
                     /label="ColE1"  
     misc_feature    6671..7465 
                     /label="Kan/Neo"  
     misc_feature    7500..7802 
                     /label="SV40 ori"  
     misc_feature    7888..8365 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4569..4575 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4569 
                     /label="Dido1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGATAAA GGGCACCTGA GCAATGAGGA AGCACCCAAG GCTATCAAAC 
     1081 CCACCAGTAA GGAGTTCAGG AAAACCTGGG GTTTTCGAAG AACCACGATT GCCAAACGTG 
     1141 AGGGTGCAGG AGACACGGAG GTGGACCCCA GTGAGCAGCA ACCACAGCAG CATAACCTCT 
     1201 CCCTGCGCCG CAGTGGACGG CAACCAAAAC GTACTGAGAG GGTAGAAGAG TTTCTTACCA 
     1261 CGGTTCGGCG CCGAGGGAAA AAGAATGTGC CGGTGTCCCT GGAGGATTCC AGTGAGCCCA 
     1321 CATCTTCCAC AGTCACTGAT GTGGAGACAG CTTCCGAGGG GAGCGTTGAA AGCAGTTCTG 
     1381 AGATCAGAAG TGGCCCTGTA TCTGACTCCT TAGGGAAAGA ACATCCTGCC TCTTCTGAAA 
     1441 AGGCAAAAGG AGGTGAAGAG GAAGAAGACA CCTCTGACAG TGACAGTGAT GGCCTTACGT 
     1501 TGAAGGAACT TCAGAACCGC CTTCGGAGAA AGCGAGAGCA AGAACCTGTG GAGAGGTCCC 
     1561 TGAGAGGCAG TCAGAATCGC CTGAGGAAGA AGCGCAGAGA GGAAGATTCT GCCGAAACTG 
     1621 GGAGTGTCCA AATAGGCAGT GCCGAGCAGG ACAGACCTCT CTGTAAGCAG GAGCCTGAGG 
     1681 CTAGTCAGGG ACCAGTGTCC CAGTCAGAGA CAGATGACAT AGAAAATCAG TTGGAAGGGA 
     1741 AGGCGACTCA GGGAAATACA GAGGAAAACC CCAGGGAAGC GGGCAAACCA AAGCCTGAGT 
     1801 GTGAGGTTTA CGACCCCAAT GCCCTGTACT GCATCTGCCG CCAGCCTCAC AACAACAGGT 
     1861 TTATGATCTG CTGTGATCGG TGTGAGGAGT GGTTCCATGG TGACTGTGTG GGTATTTCTG 
     1921 AGGCCCGAGG GCGGCTCCTG GAAAGGAACG GGGAAGACTA CATCTGCCCA AATTGCACCA 
     1981 TTTTGCAAGT GCAGGATGAG ACAAACGGTA GCGCCACCGA TGAGCAGGAC TCTGGGTGCA 
     2041 GATCTGTGGG TGCTGATGGC ACAGACTGCA CAAGCATAGG GACAGTAGAG CAGAAGTCCG 
     2101 GAGAAGACCA GGGCATAAAG GGTAGGATTG AGAAGGCAGC AAACCCCAGC GGCAAGAAAA 
     2161 AACTCAAGAT ATTCCAGCCT GTCGTAGAGG CTCCTGGTGC TCCTAAATGC ATTGGCCCTG 
     2221 GGTGTTCCAG TGTAGCACAG CCTGACTCTG TGTATTGCAG TAATGACTGC ATTCTCAAAC 
     2281 ACGCAGCAGC TACCATGAGA TTTCTAAGTT CAGGTAAAGA ACAAAAAACA AAACCCAAGG 
     2341 AAAAGGTCAA GACGAAGCCA GAAAAGTTCA GTCTTCCAAA ATGCAGTGTT CAGGTGGGGA 
     2401 TTAAAATCTC TTCTGTGCAC AAGAGACTAG CGTCAGAGAA AAGGGAAAAC CCAGTGAAGA 
     2461 AAGTGATGCT GGCTTCCAGG AGTGAGACTT CTGGGAAGGA GGCAGCCTGT GAGAGCAGCA 
     2521 CACCATCCTG GGCAAGTGAC CACAACTACA ATGCTGTGAA GCCAGAGAAG CCAGAGAAGC 
     2581 CCACTGCACT CTCGCCCACC CTATTGAGTA AATCCATGAA GGATGACAGG AGGGTGGAGG 
     2641 ACAGGACAAT GGCAGCAGTT ACCATCCCGA AGAAAGCACT TCCTTCAGCC TCTTTGGTGG 
     2701 GCAGACAGAC TTCACCTAGG AATCTTGTTC CAAAGAAGCT TCCTCCTTAC TCTAATATGG 
     2761 CAGGAGCCAA ACCAGCCATT AAGAAACTGC CTTCAGGCTT CAAGGGTACC ATCCCCAAGA 
     2821 GGCCATGGCC CTCAGCCACC CTGTCAGGCA CTTCTGCCAG GCAGGCAGGA CCAACACCTA 
     2881 TGACAGCTGC TTCCAAAAAG TTGCCTGGCT CTGCTGCTGT GGTGGGAGTT ACCAGGAAGC 
     2941 CAATGTCTGC CAATGTTCCT GCTGCTTCTC CAGCCCCAGG ACGGCTTGGT CCTGTGAGCC 
     3001 CAGCTCCATC ACAGCCCAAT TCACAAATTC GACAAAATAT AAGGCGCTCC TTGAAAGAGA 
     3061 TTTTGTGGAA AAGAGTCAAT GACAGCGATG ACTTAATAAT GACAGAAAAT GAAGTAGGAA 
     3121 AAATTGCCCT CCACATTGAG AAGGAGATGT TTAACTTGTT CCAGGTTACT GATAATCGCT 
     3181 ATAAGAGCAA ATATCGCAGC ATCATGTTCA ACCTTAAGGA TCCTAAGAAT CAGGGGCTCT 
     3241 TCCATCGTGT TCTTCGAGAA GAAATCTCCT TGGCAAAACT TGTCAGAATG AAGCCTGAAG 
     3301 AACTTGTATC TAAAGAGCTT TCTATGTGGA CAGAGAAGCC CACAAAATCT GTGATAGAAT 
     3361 CCAGGACTAA GTTGCTTAAT GAAAGCAAGA AGAACACTAC TAAACCAGAA ACCATTCCTG 
     3421 ACATGGAAGA TTCTCCACCG GTATCAGATT CAGAAGAACA ACAAGAGTCA GTGCGAGCCG 
     3481 CCCCTGAGAA GAGCGCAGCA CCTCTCCTGG ACGTCTTCAG CAGCATGCTG AAGGACACCA 
     3541 CAAGCCAGCA CCGTGCCCAT CTTTTTGATT TAAACTGTAA AATCTGTACA GGTCAGGTTC 
     3601 CATCCTCGGA GGATGAACCA GCTCCTAAGA AGCAAAAGCT TTCAGCTTCT TCTAAGAAGG 
     3661 AAGACTTTAA GCCCAGGCAT GACAGCTCTC CACCTAATGC AGTTCCTAAC ACTGCTGATG 
     3721 AAGGGATTGC AGACACGCTG CCTGAAAATG CCTCTGAGCC AGACCCGGAG AGTACATCTA 
     3781 GTCTTAACCA GGAGAGAAAG TGTTTCCCTG AGTCTCCAGG CGATAGCCAT CCTGAGCCCT 
     3841 CGTCTCTGGG TGGCCTTTCT CCCTCTTCTG CCTCTGGTGG GAGTGGGGTG GTCACCACAG 
     3901 TCACCATGTC TGGTCGAGAC CCCAGAACTG CCCTGAGTGG GTCGTGCACA GTCACAGCCT 
     3961 CCATGGCAGC CCATCTGGAC AACTCCCAGG CTTCAGAAAC CAAACTGGAC ATGATAAAGC 
     4021 CTGCATTGAC TTCTGCAGTG GTGCCCAAGT CCATACTGGC TAAGCCATCC TCCTCTCCTG 
     4081 ACCCGAGATA CCTGTCAGTA CCACCATCAC CAAGCATTTC AGAATCACGA TCCCCACCAG 
     4141 AAGGAGATAC AACCCTCTTT TTGTCTCGAC TCAACACGAT TTGGAAAGGA TTTATTAATA 
     4201 TGCAGAGTGT AGCAAAATTT GTCACTAAGG CATATCCTGT CTCTGGGTGT TTGGATTATC 
     4261 TCAGTGAGGA TTTGCCAGAC ACCATCCACA TCGGTGGAAG GATTGCTCCA AAGACCGTGT 
     4321 GGGATTACGT TGGCAAACTC AAGTCATCGG TGTCCAAGGA GCTCTGTCTG ATCCGCTTCC 
     4381 ACCCTGCCAC TGAGGAGGAG GAGGTTGCAT ACATTTCTCT CTACTCCTAT TTTAGCAGCC 
     4441 GTGGTCGCTT TGGGGTTGTA GCTAATAACA ACAGGCATGT CAAGGACCTG TACCTGATTC 
     4501 CACTGAGTGC TAAGGACCCT GTGCCATCCA AACTCTTGCC CTTTGAGGGC CCAGGTAAGC 
     4561 ACCCAGTTTC TGGGAGGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4621 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4681 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4741 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4801 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4861 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4921 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4981 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5041 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5101 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5161 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5221 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5281 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5341 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5401 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5461 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5521 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5581 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5641 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5701 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5761 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5821 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5881 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5941 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6001 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6061 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6121 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6181 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6241 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6301 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6361 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6421 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6481 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6541 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6601 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6661 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6721 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6781 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6841 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6901 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6961 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7021 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7081 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7141 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7201 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7261 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7321 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7381 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7441 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7501 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7561 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7621 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7681 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7741 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7801 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7861 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7921 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7981 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8041 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8101 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8161 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8221 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8281 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8341 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8401 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//