LOCUS       MR223970                6984 bp    DNA     circular  SYN 
DEFINITION  Alox12b (Myc-DDK-tagged) - Mouse arachidonate 12-lipoxygenase, 12R type (Alox12b)
ACCESSION   NM_009659
VERSION     NM_009659.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3141..3232 
                     /label="Myc-DDK"  
     misc_feature    3262..3847 
                     /label="PolyA signal"  
     misc_feature    3319..3338 
                     /label="Restriction Enzyme"  
     misc_feature    4123..4795 
                     /label="ColE1"  
     misc_feature    5225..6019 
                     /label="Kan/Neo"  
     misc_feature    6054..6356 
                     /label="SV40 ori"  
     misc_feature    6442..6919 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3123..3129 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3123 
                     /label="Alox12b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACCTAT AAAGTCAAGG TGGCCACGGG CACTGACTTC TTTTCGGGGA 
     1081 CGCTGGATTC AATTTCACTG ACCATCGTGG GGACCCAAGG AGAGAGCCAT AAGCAGCGGT 
     1141 TGAACCACTT TGGGAGAGAC TTTGCCACGG GGGCGGTGGA TGACTACACG GTACAGTGCC 
     1201 AGCAGGACCT GGGGGAGCTT ATCATCATCC GTCTGCACAA GGAGCCTCAT TCCTTCCTAG 
     1261 CCAAGGACCC CTGGTACTGC AACTATGTGC AGATCTGTGC TCCCGACTGC CGTGTCTACC 
     1321 ACTTCCCAGC TTACCAGTGG ATGGATGGCT ATGAGACCCT GGCACTCCGG GAGGCCACAG 
     1381 GAAAGATAAC AGCAGACGAC ACACTCCCTA TCCTTTTGGA ACACAGACAA GAAGAGATCA 
     1441 GAGCCAAGAA GGACTTCTAC CACTGGAGGG TCTTTGTTCC TGGCCTTCCC AACTATGTGG 
     1501 ATATCCCCAG TTACCATCCT CCTCCCCGAC GGTGCCGCAA TCCCAACCGG CCTGAGTGGG 
     1561 ATGGTTATAT TCCCGGATTC CCAATTCTCA TCAACATTAA GGCTACCAGG TTCTTAAACT 
     1621 CAAATCTTCG TTTCTCCTTC GTCAAGACAG CCTCCTTCTT CTACCGCCTA GGGCCCATGG 
     1681 CGCTGGCCTT CAAACTGCGT GGCCTGGTGG ATCGAAAACG CTCCTGGAAG AGACTAAAGG 
     1741 ACATTAAGAA TATTTTCCCT GCTACCAAGT CTGTCGTCTC AGAGTATGTG GCCGAGCACT 
     1801 GGACAGAGGA CAGCTTCTTT GGGTACCAGT ACCTTAATGG TATTAACCCA GGCCTGATCC 
     1861 GAAGATGTAC ACAGATCCCA GACAAATTCC CAGTCACAGA TGAAATGGTG GCCCCATTCC 
     1921 TGGGTGAAGG AACATGCCTG CAAGCAGAGC TGGAGAGGGG GAATATTTAC CTGGCAGACT 
     1981 ACCGCATCCT GGATGGCATC CCCACCGTGG AGCTCAATGG TCAGCAGCAG CATCACTGTG 
     2041 CCCCGATGTG CTTGCTGCAC TTTGGTCCTG ATGGCAACAT GATGCCCATC GCCATTCAGC 
     2101 TCAGTCAGAC TCCTGGGCCT GATTGTCCTA TCTTCCTGCC GAATGATTCT GAGTGGGACT 
     2161 GGCTGTTGGC TAAAACGTGG GTGCGCTACG CGGAGTTCTA CAGCCACGAG GCCGTGGCAC 
     2221 ACTTGCTGGA GAGCCACCTC ATCGGGGAAG CTTTCTGCTT GGCCCTCCTG AGGAACCTGC 
     2281 CCATGTGCCA CCCCCTGTAT AAGCTTCTCA TCCCTCACAC TCGGTACAAC GTCCAGATCA 
     2341 ATAGCATTGG GCGGGCCCTC CTTCTCAACA AGGGAGGTCT CTCTGCCAGG GCCATGTCCC 
     2401 TGGGCCTAGA GGGCTTCGCA CAGGTGATGG TTCGGGGTCT GTCTGAGCTC ACCTACAAAA 
     2461 GCCTCTGCAT TCCCAACGAC TTTGTGGAGC GCGGGGTCCA GGACCTGCCT GGGTATTATT 
     2521 TCCGTGATGA CAGCCTGGCG GTGTGGTACG CGATGGAGAG GTACGTGACA GAGATCATCA 
     2581 CTTATTATTA CCCAAATGAC GCAGCCGTAG AGGGCGACCC GGAACTGCAG TGCTGGGTAC 
     2641 AGGAGATATT CAAGGAGTGT CTCCTGGGGC GTGAGAGCTC AGGCTTCCCC ACCTGCTTGC 
     2701 GAACTATACC TGAGCTGATC GAATACGTCA CAATGGTCAT GTACACCTGC TCGGCCAGGC 
     2761 ATGCAGCTGT CAACTCAGGC CAGCTGGAGT ACACCTCTTG GATGCCCAAC TTCCCATCAT 
     2821 CCATGAGGAA CCCACCGATG CAGACCAAGG GGCTAACTAC TCTGCAGACA TACATGGACA 
     2881 CTCTTCCGGA CGTGAAGACC ACATGCATTG TGCTCTTGGT GCTCTGGACT CTCTGTCGGG 
     2941 AGCCTGATGA CAGGCGGCCG CTTGGCCACT TCCCAGACAT CCATTTTGTG GAGGAGGGTC 
     3001 CTCGGAGGAG TATTGAGGCT TTTCGTCAGA ACCTCAACCA GATCTCTCAC AACATCCGAC 
     3061 AGCGCAATAA GTGCCTCACC CTCCCGTACT ACTACCTGGA CCCAGTGCTG ATTGAGAACA 
     3121 GTATTTCCAT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3181 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3241 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3301 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3361 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3421 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3481 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3541 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3601 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3661 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3721 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3781 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3841 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3901 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3961 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4021 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4081 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4141 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4201 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4261 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4321 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4381 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4441 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4501 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4561 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4621 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4681 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4741 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4801 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4861 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4921 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4981 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5041 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5101 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5161 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5221 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5281 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5341 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5401 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5461 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5521 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5581 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5641 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5701 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5761 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5821 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5881 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5941 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6001 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6061 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6121 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6181 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6241 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6301 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6361 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6421 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6481 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6541 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6601 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6661 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6721 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6781 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6841 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6901 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6961 TAACAAAAAT TTAACGCGAA TTTT
//