LOCUS       MR223953                7608 bp    DNA     circular  SYN 
DEFINITION  Nrp2 (Myc-DDK-tagged) - Mouse neuropilin 2 (Nrp2), transcript variant 4
ACCESSION   NM_001077405
VERSION     NM_001077405.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3765..3856 
                     /label="Myc-DDK"  
     misc_feature    3886..4471 
                     /label="PolyA signal"  
     misc_feature    3943..3962 
                     /label="Restriction Enzyme"  
     misc_feature    4747..5419 
                     /label="ColE1"  
     misc_feature    5849..6643 
                     /label="Kan/Neo"  
     misc_feature    6678..6980 
                     /label="SV40 ori"  
     misc_feature    7066..7543 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3747..3753 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3747 
                     /label="Nrp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATATGTTT CCTCTTACCT GGGTTTTCTT AGCTCTGTAC TTTTCAGGAC 
     1081 ACGAAGTGAG AAGCCAGCAA GATCCACCCT GCGGAGGTCG GCTGAATTCC AAAGATGCTG 
     1141 GCTACATCAC TTCCCCAGGC TACCCCCAGG ACTATCCCTC CCACCAGAAC TGTGAGTGGA 
     1201 TTGTCTACGC CCCCGAACCC AACCAGAAGA TTGTTCTCAA CTTCAACCCT CACTTTGAAA 
     1261 TCGAGAAACA CGACTGCAAG TATGACTTCA TTGAGATTCG GGATGGGGAC AGTGAGTCAG 
     1321 CTGACCTCCT GGGCAAGCAC TGTGGGAACA TCGCCCCGCC CACCATCATC TCCTCAGGCT 
     1381 CCGTGTTATA CATCAAGTTC ACCTCAGACT ACGCCCGGCA GGGGGCAGGT TTCTCTCTAC 
     1441 GCTATGAGAT CTTCAAAACA GGCTCTGAAG ATTGTTCCAA GAACTTTACA AGCCCCAATG 
     1501 GGACCATTGA ATCTCCAGGG TTTCCAGAGA AGTATCCACA CAATCTGGAC TGTACCTTCA 
     1561 CCATCCTGGC CAAACCCAGG ATGGAGATCA TCCTACAGTT CCTGACCTTT GACCTGGAGC 
     1621 ATGACCCTCT ACAAGTGGGG GAAGGAGACT GTAAATATGA CTGGCTGGAC ATCTGGGATG 
     1681 GCATTCCACA TGTTGGACCT CTGATTGGCA AGTACTGTGG GACGAAAACA CCCTCCAAAC 
     1741 TCCGCTCGTC CACGGGGATC CTCTCCTTGA CCTTTCACAC GGACATGGCA GTGGCCAAGG 
     1801 ATGGCTTCTC CGCACGTTAC TATTTGATCC ACCAGGAGCC ACCTGAGAAT TTTCAGTGCA 
     1861 ATGTCCCTTT GGGAATGGAG TCTGGCCGGA TTGCTAATGA ACAGATCAGT GCCTCCTCCA 
     1921 CCTTCTCTGA TGGGAGGTGG ACTCCTCAAC AGAGCCGGCT CCATGGTGAT GACAATGGCT 
     1981 GGACACCCAA TTTGGATTCC AACAAGGAGT ATCTCCAGGT GGACCTGCGC TTCCTAACCA 
     2041 TGCTCACAGC CATTGCAACA CAGGGAGCCA TTTCCAGGGA AACCCAGAAA GGCTACTACG 
     2101 TCAAATCGTA CAAGCTGGAA GTCAGCACAA ATGGTGAAGA TTGGATGGTC TACCGGCATG 
     2161 GCAAAAACCA CAAGATATTC CAAGCGAACA ATGATGCGAC CGAGGTGGTG CTAAACAAGC 
     2221 TCCACATGCC ACTGCTGACT CGGTTCATCA GGATCCGCCC GCAGACGTGG CATTTGGGCA 
     2281 TTGCCCTTCG CCTGGAGCTC TTTGGCTGCC GGGTCACAGA TGCACCCTGC TCCAACATGC 
     2341 TGGGGATGCT CTCGGGCCTC ATTGCTGATA CCCAGATCTC TGCCTCCTCC ACCCGAGAGT 
     2401 ACCTCTGGAG CCCCAGTGCT GCCCGCCTGG TTAGTAGCCG CTCTGGCTGG TTTCCTCGGA 
     2461 ACCCTCAAGC CCAGCCAGGT GAAGAATGGC TTCAGGTAGA CCTGGGGACA CCCAAGACAG 
     2521 TGAAAGGGGT CATCATCCAG GGAGCCCGAG GAGGAGACAG CATCACTGCC GTGGAAGCCA 
     2581 GGGCGTTTGT ACGCAAGTTC AAAGTCTCCT ACAGCCTAAA TGGCAAGGAC TGGGAATATA 
     2641 TCCAGGACCC CAGGACTCAG CAGACAAAGC TGTTTGAAGG GAACATGCAC TATGACACCC 
     2701 CTGACATCCG AAGGTTCGAT CCTGTTCCAG CGCAGTATGT GCGGGTGTAC CCAGAGAGGT 
     2761 GGTCGCCAGC AGGCATCGGG ATGAGGCTGG AGGTGCTGGG CTGTGACTGG ACAGACTCAA 
     2821 AGCCCACAGT GGAGACGCTG GGACCCACCG TGAAGAGTGA AGAGACTACC ACCCCATATC 
     2881 CCATGGATGA GGATGCCACC GAGTGTGGGG AAAACTGCAG CTTTGAGGAT GACAAAGATT 
     2941 TGCAACTTCC TTCAGGATTC AACTGCAACT TTGATTTTCC GGAAGAGACC TGTGGTTGGG 
     3001 TGTACGACCA TGCCAAGTGG CTCCGGAGCA CGTGGATCAG CAGCGCTAAC CCCAATGACA 
     3061 GAACATTTCC AGATGACAAG AACTTCTTGA AACTGCAGAG TGATGGCCGA CGAGAGGGCC 
     3121 AGTACGGGCG GCTCATCAGC CCACCGGTGC ACCTGCCCCG AAGCCCTGTG TGCATGGAGT 
     3181 TCCAGTACCA AGCCATGGGC GGCCACGGGG TGGCACTGCA GGTGGTTCGG GAAGCCAGCC 
     3241 AGGAAAGCAA ACTCCTTTGG GTCATCCGTG AGGACCAGGG CAGCGAGTGG AAGCACGGGC 
     3301 GCATTATCCT GCCCAGCTAT GACATGGAGT ATCAGATCGT GTTCGAGGGA GTGATAGGGA 
     3361 AGGGACGATC GGGAGAGATT TCCATCGATG ACATTCGGAT AAGCACTGAT GTCCCACTGG 
     3421 AGAACTGCAT GGAACCCATA TCAGCTTTTG CAGATGAATA TGAAGGAGAT TGGAGCAACT 
     3481 CTTCTTCCTC TACCTCAGGG GCTGGTGACC CCTCATCTGG CAAAGAAAAG AGCTGGCTGT 
     3541 ACACCCTAGA TCCCATTCTG ATCACCATCA TCGCCATGAG CTCGCTGGGG GTCCTGCTGG 
     3601 GGGCCACCTG TGCGGGCCTC CTCCTTTACT GCACCTGCTC CTATTCGGGT CTGAGTTCGA 
     3661 GGAGCTGCAC CACACTGGAG AACTACAACT TTGAGCTCTA CGATGGCCTC AAGCACAAGG 
     3721 TCAAGATCAA TCATCAGAAG TGCTGCTCGG AGGCAACGCG TACGCGGCCG CTCGAGCAGA 
     3781 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3841 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3901 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3961 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4021 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4081 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4141 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4201 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4261 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4321 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4381 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4441 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4501 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4561 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4621 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4681 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4741 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4801 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4861 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4921 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4981 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5041 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5101 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5161 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5221 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5281 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5341 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5401 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5461 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5521 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5581 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5641 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5701 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5761 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5821 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5881 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5941 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6001 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6061 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6121 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6181 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6241 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6301 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6361 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6421 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6481 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6541 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6601 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6661 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6721 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6781 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6841 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6901 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6961 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7021 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7081 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7141 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7201 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7261 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7321 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7381 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7441 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7501 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7561 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//