LOCUS       MR223945                8817 bp    DNA     circular  SYN 
DEFINITION  Frmpd4 (Myc-DDK-tagged) - Mouse FERM and PDZ domain containing 4 (Frmpd4)
ACCESSION   NM_001033330
VERSION     NM_001033330.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4974..5065 
                     /label="Myc-DDK"  
     misc_feature    5095..5680 
                     /label="PolyA signal"  
     misc_feature    5152..5171 
                     /label="Restriction Enzyme"  
     misc_feature    5956..6628 
                     /label="ColE1"  
     misc_feature    7058..7852 
                     /label="Kan/Neo"  
     misc_feature    7887..8189 
                     /label="SV40 ori"  
     misc_feature    8275..8752 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4956..4962 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4956 
                     /label="Frmpd4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGTCACAC AGCTGCCACA GGACCAAGTC TTCGGGCTGG CCACCTCCCT 
     1081 CAGGAACCTG GGGCTTGAAC CAGGTACCAC CCTATGGATG GGAGATGATG ACAAACCGAG 
     1141 ATGGACGTGA CTACTTCATC AATCACATGA CACAGGCAAT CCCATTTGAT GACCCTCGGT 
     1201 TTGACAGCTG CCAAATCATC CCCCCAGCTC CACGAAAGGT GGAGATGAGG AGAGACCCTG 
     1261 TGCTGGGCTT TGGGTTTGTG GCAGGGAGCG AAAAGCCAGT GGTCGTTCGG TCAGTAACAC 
     1321 CAGGTGGCCC CTCAGAAGGC AAGCTGATCC CAGGAGATCA GATTGTAATG ATTAATGATG 
     1381 AAGCAGTCAG CGCTGCACCA AGAGAGAGAG TCATCGACCT GGTCAGGAGC TGCAAAGAAT 
     1441 CGATTCTGCT CACTGTCATT CAGCCCTATC CATCTCCCAA ATCAGCATTT ATCAGTGCTG 
     1501 CTAAAAAGGC AAGATTGAAG TCCAATCCAG TCAAAGTGCG CTTTTCCGAA GAGGTCATAA 
     1561 TCAATGGCCA AGTGTCGGAA ACTGTTAAAG ACAATTCACT TCTTTTTATG CCAAATGTTT 
     1621 TGAAAGTCTA TTTGGAAAAC GGACAGACCA AATCCTTTCG TTTTGACTGC AGCACCTCCA 
     1681 TCAAGGATGT CATCTTAACC CTGCAAGAGA AGTTGTCTAT CAAAGGCATT GAACACTTCT 
     1741 CCCTCATGCT GGAGCAGAGA ATTGAAGGGG CTGGCACCAA GCTGCTCTTG CTTCATGAAC 
     1801 AGGAGACACT CACTCAGGTG ACACAGAGGC CCAGCTCCCA TAAGATGAGG TGTCTTTTCC 
     1861 GAATCAGTTT TGTTCCCAAA GATCCCATTG ACCTGTTAAG AAGAGATCCA GTTGCTTTTG 
     1921 AGTATCTCTA TGTTCAGAGT TGTAACGATG TCGTTCAGGA GCGATTTGGA CCAGAGCTGA 
     1981 AATATGACAT TGCCTTGCGG CTGGCCGCGT TACAAATGTA CATTGCTACT GTCACCACCA 
     2041 AACAGACGCA GAAAATCTCC CTCAAATACA TTGAGAAAGA ATGGGGACTA GAGACTTTTC 
     2101 TTCCATCTGC TGTGCTGCAG AGCATGAAAG AGAAGAACAT CAAGAAAGCA CTCTCCCACC 
     2161 TTGTCAAAGC AAATCAAAAC TTGGTACCAC CGGGTAAAAA GCTCTCTGCA CTACAAGCTA 
     2221 AGGTCCACTA TCTCAAGTTC CTCAGCGACC TGCGACTATA TGGGGGCCGT GTATTCAAGG 
     2281 CAACATTAGT GCAGGCAGAG AAGCGCTCAG AAGTAACTCT TCTGGTTGGT CCCCGGTATG 
     2341 GCATAAGCCA TGTCATAAAC ACCAAAACCA ATCTGGTGGC TCTTTTAGCT GACTTCAGCC 
     2401 ATGTCAACAG GATTGAAATG TTTACTGAAG AGGAGAGCTT GGTGAGGGTG GAGTTGCATG 
     2461 TGCTAGATGT GAAGCCCATT ACACTACTTA TGGAGTCATC AGACGCCATG AACCTGGCCT 
     2521 GTCTGACAGC TGGATACTAC CGGCTGCTTG TGGACTCCAG GAGGTCAATA TTTAACATGG 
     2581 CCAACAAGAA AAACGCAGGC ACGCAGGACA CAGGATCAGA AAATAAAGGC AAGCATAATC 
     2641 TCCTTGGTCC TGATTGGAAC TGTATGCCCC AGATGACCAC CTTCATTGGT GAAGGCGAAC 
     2701 AAGAAGCCCA AATCACATAT ATAGATTCTA AGCAGAAGAC AGTTGAGATG ACAGACAGCA 
     2761 CCTTGTGTCC CAAAGAGCAC CGGCACTTAT ATATCGACAA CTCATACAGT TCAGATGAAC 
     2821 TTAACCAGCC GCTGACTCAG CCAGGTGATG CCCCCTGTGA GGCCGACTAT AGAAGTCTAG 
     2881 CTCAGCGGTC CCTTTTGACC CTCTCAGGAC CAGACACTCT GAAGAAAGCA CAGGAATCTC 
     2941 CGCGAGGAGC TAAAGTGTCC TTTATTTTTG GAGATCTTGC CTTAGATGAT GGCATGAGTC 
     3001 CCCCAACTAT AGGCTATGAA AGAATGTTAG AGGAGAATCC AGAAATGCTG GAGAAGCAGA 
     3061 GGAATCTCTA CATCAGCAGT GCCAATGACA TGAAGAACCT GGACCTCACT CCAGACACAG 
     3121 ACAGCATCCA GTTTGTGGCA AATTCTGTAT ATGCAAACAT AGGTGATGTG AAGAACTTTG 
     3181 AGGCCCCTGA GGGGATAGAG GAGCCCCTCT TACATGACAT CTGTTATGCA GAAAATACGG 
     3241 ACGATGCAGA AGATGAAGAT GAGGTGAGCT GCGAGGAGGA CCTCGTGGTG GGTGAAATGA 
     3301 ACCAACCAGC CATCCTTGAC CTGTCCGGGT CAAGTGATGA CATTATTGAC CTCACAACCC 
     3361 TGCCTCCTCC AGAAGGAGAT GACAATGAGG ATGACTTCCT CCTGCGTTCT CTGAACATGG 
     3421 CCATCGCTGC TCCCCCACCT GGCTTTAGAG ACAGTTCTGA TGAAGAGGAC ACTCAGAGCC 
     3481 AGGCAACGTC CTTCCATGAG GACAAAGAAC AAGGCAGCAG CCTGCAGAAT GAGGAGATCC 
     3541 CTGTGTCCCT CATTGATGCT GTGCCCACCA GTGCAGAGGG CAAGTGTGAG AAGGGACTGG 
     3601 ACCCTGCCGT CGTTTCCACA CTGGAAGCCC TAGAAGCTCT TTCAGAAGAA CAGCAGAAGA 
     3661 GTGAAAATTC AGGTGTAGCC ATCTTGCGAG CTTATAGTCC CGAGTCTTCA TCAGACTCGG 
     3721 GCAATGAGAC TAACTCTTCT GAAATGACAG AGGGTTCTGA GCTAGCTGCA GCACAGAAGC 
     3781 AGTCGGAAAG CCTCTCCCGC ATGTTCTTGG CCACTCATGA AGGCTATCAC CCTCTGGCAG 
     3841 AAGAGCAGAC AGAGTTCCCC ACCTCCAAAG CCCCCTCAGT GGGCTTGCCT CCAAAGTCCT 
     3901 CCCATGGCCT GGCTGCTCGC CCAGCGACCG ACCTCCCACC CAAAGTTGTG CCTTCCAAGC 
     3961 AGATCCTTCA CTCAGACCAC ATGGAAATGG AGCCAGAAAC CATGGAGACC AAGTCTGTCA 
     4021 CTGACTATTT TAGCAAACTG CATATGGGGT CAGTAGCATA TTCCTGTACC AGCAAAAGGA 
     4081 AAAGCAAGCT TCCTGAGGGA GAGGGGAAAT CCCCCCTGAG TGGGAATATA CCAGGGAAAA 
     4141 AACAGCAGGG AACCAAAATA GCAGAGATAG AGGAGGACAC CAAAGGCAAA GCTGGCACTG 
     4201 TGTCTTCAAG AGACAATCCA CACCTCAGCA CTTTTAACCT AGAGAGAACT GCCTTTCGCA 
     4261 AGGACAGCCA AAGATGGTAT GTGGCCTCTG ATGGTGGGGT GGTAGAGAAA AGTGGAATGG 
     4321 AAGCACCAGC CATGAAAGTC TTTCCCAGAG GTCCAGGCTT GGGTAACAGA GAGGCTGAAG 
     4381 GGAAAGAGGA TGGCACTGTG GAAGGAGGGG CTGATGATGC TTCAGTACTT GGCCAAGGGG 
     4441 ACCGCTTCCT CACAGACATG GCCTGTGTAG CCTCAGCCAA AGACTTAGAT AACCCCGAAG 
     4501 ATACTGACTC TCCCTCTTGT GACCATGCCA CTAAGCTTTC TGAGGCTGAA GACAATGTGG 
     4561 CCCGCCTTTG TGACTACCAT TTGGCCAAGC GAATGTCATC CCTGCAGAGT GAGGGCCATT 
     4621 TTTCTCTACA GAGCTCTCAA GGCTCTTCAG TGGACACAGG CTGTGGACCG GGCAGCAGTA 
     4681 GCAGTGCCTG TGCCACTCCT GTGGAATCGC CCCTCTGCCC GTCCATGGGG AAGCACATGA 
     4741 TTCCAGATGC TTCTGGGAAA GGAGGGAGAT ATATTTCACC AGAGGAGAGA GCCCCTGGCC 
     4801 ATCCCAACCA TGGAGCCACC TTCGAGGAAC TGCACCCACA GACAGAAGGG ATGTGTCCAC 
     4861 GCATGACAGT GCCTGCTCTG CACACAGCCA TTAATGCCGA CCCCCTGTTT GGCACTTTGA 
     4921 GAGATGGATG CCATCGACTG CCCAAGATTA AGGAAACCAC AGTGACGCGT ACGCGGCCGC 
     4981 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5041 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5101 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5161 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5221 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5281 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5341 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5401 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5461 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5521 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5581 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5641 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5701 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5761 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5821 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5881 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5941 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6001 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6061 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6121 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6181 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6241 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6301 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6361 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6421 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6481 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6541 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6601 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6661 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6721 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6781 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6841 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6901 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6961 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7021 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7081 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7141 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7201 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7261 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7321 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7381 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7441 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7501 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7561 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7621 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7681 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7741 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7801 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7861 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7921 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7981 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8041 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8221 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8281 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8341 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8401 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8461 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8521 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8581 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8641 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8701 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8761 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//