LOCUS       MR223913                8892 bp    DNA     circular  SYN 
DEFINITION  Mink1 (Myc-DDK-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 3
ACCESSION   NM_001045959
VERSION     NM_001045959.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5049..5140 
                     /label="Myc-DDK"  
     misc_feature    5170..5755 
                     /label="PolyA signal"  
     misc_feature    5227..5246 
                     /label="Restriction Enzyme"  
     misc_feature    6031..6703 
                     /label="ColE1"  
     misc_feature    7133..7927 
                     /label="Kan/Neo"  
     misc_feature    7962..8264 
                     /label="SV40 ori"  
     misc_feature    8350..8827 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5031..5037 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5031 
                     /label="Mink1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACCCA GCCCCCGCCC GCAGCCTGGA CGACATCGAC CTGTCTGCCC 
     1081 TGCGGGACCC TGCAGGAATC TTTGAGCTGG TGGAGGTGGT TGGCAATGGA ACCTATGGAC 
     1141 AGGTATACAA GGGGCGGCAC GTCAAGACTG GGCAGCTGGC TGCCATTAAG GTCATGGATG 
     1201 TCACAGAGGA TGAGGAGGAA GAGATCAAAC AGGAAATCAA CATGTTAAAG AAGTACTCTC 
     1261 ACCATCGCAA TATTGCCACC TACTATGGGG CCTTTATCAA GAAGAGCCCT CCTGGGAACG 
     1321 ATGACCAGCT CTGGCTGGTG ATGGAGTTCT GCGGTGCTGG TTCAGTGACC GACCTGGTAA 
     1381 AGAACACAAA AGGGAACGCA CTGAAGGAGG ATTGCATTGC TTACATCTGC AGGGAGATTC 
     1441 TCAGGGGTCT TGCCCATCTC CATGCCCACA AGGTGATCCA CAGAGATATC AAGGGACAAA 
     1501 ATGTGCTGCT GACAGAGAAT GCTGAAGTCA AGCTAGTGGA TTTTGGGGTG AGTGCTCAGC 
     1561 TGGACCGCAC TGTGGGCAGG CGGAACACTT TCATTGGAAC CCCATACTGG ATGGCTCCAG 
     1621 AAGTCATTGC CTGTGACGAG AACCCCGATG CCACCTATGA CTACAGGAGT GACATTTGGT 
     1681 CTCTAGGAAT CACAGCCATT GAAATGGCAG AGGGAGCCCC CCCTCTGTGT GACATGCACC 
     1741 CTATGCGGGC CCTCTTCCTC ATCCCTCGGA ACCCTCCCCC CAGGCTCAAG TCAAAGAAAT 
     1801 GGTCTAAGAA GTTCACTGAC TTCATCGACA CGTGTCTCAT CAAGACTTAC CTGAGCCGCC 
     1861 CACCCACCGA ACAGTTACTC AAATTCCCCT TCATCCGAGA CCAGCCCACG GAGCGGCAGG 
     1921 TCCGCATCCA GCTCAAGGAC CACATCGACC GCTCGCGGAA GAAGCGGGGT GAGAAAGAGG 
     1981 AGACAGAGTA TGAGTACAGC GGCAGTGAGG AGGAAGACGA CAGCCATGGA GAGGAAGGCG 
     2041 AGCCAAGCTC CATCATGAAT GTGCCCGGTG AGTCCACACT GCGCAGAGAA TTCCTCAGAC 
     2101 TCCAGCAGGA GAATAAGAGC AACTCTGAGG CTTTAAAGCA GCAGCAGCAG CTGCAGCAAC 
     2161 AGCAGCAGCG GGACCCGGAG GCACACATCA AACACCTGCT GCACCAGCGG CAGCGTCGCA 
     2221 TAGAGGAGCA GAAGGAGGAG CGGCGACGTG TGGAGGAGCA ACAGCGGCGA GAGCGAGAAC 
     2281 AGCGTAAGCT ACAAGAGAAG GAGCAGCAGC GGCGATTGGA AGACATGCAA GCCCTACGAC 
     2341 GAGAGGAAGA GAGGCGGCAA GCAGAGCGGG AACAGGAATA CAAGCGGAAG CAGCTGGAGG 
     2401 AGCAGCGGCA GTCAGAGCGG CTGCAGAGAC AGCTGCAGCA GGAGCACGCC TACCTCAAGT 
     2461 CCCTGCAGCA GCAGCAGCAG CAGCAGCAGC TCCAGAAGCA GCAGCAGCAG CAGCAGCAGA 
     2521 TCCTGCCTGG AGACAGGAAG CCCCTGTATC ATTACGGTCG GGGCATTAAT CCTGCTGACA 
     2581 AGCCAGCATG GGCCCGCGAG GTGGAAGAGA GAGCACGGAT GAACAAGCAG CAGAACTCTC 
     2641 CCTTGGCGAA GGCGAAGCCA AGCAGTGCGG GGCCAGAGCC CCCCATCTCC CAGGCCTCTC 
     2701 CTAGCCCCCC AGGACCTCTT TCCCAGACTC CTCCTATGCA GAGGCCTGTG GAGCCCCAGG 
     2761 AAGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT GCAGCACCTG 
     2821 TACCCCGATC CCAGTCCCTG CAGGACCAGC CGACTCGAAA CCTGGCTGCC TTCCCAGCCT 
     2881 CCCACGACCC TGACCCTGCT GCTGTCCCTA CACCCACTGC CACACCCAGT GCCCGAGGAG 
     2941 CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGGCC AGGGCCTAGC CCAAACCCTC 
     3001 CATCCTGGGT TCGGCCTGAT AATGAGGCTC CACCTAAGGT TCCACAGAGG ACCTCTTCTA 
     3061 TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCTCAG GCTGTCCGTG 
     3121 CCAGACCTCG CAGTAACTCC GCCTGGCAAA TCTATCTGCA GAGGCGGGCA GAGCGGGGCA 
     3181 CCCCCAAGCC TCCCGGGCCC CCAGCTCAGC CCCCTGGCCC GCCCAACGCC TCTAGTAACC 
     3241 CTGACCTCAG GAGGAGTGAC CCTGGCTGGG AGCGCTCAGA CAGTGTCCTC CCGGCCTCCC 
     3301 ACGGCCACCT CCCTCAGGCT GGCTCCTTGG AGCGGAACCG AAACCGTGTG GGAGCCTCCA 
     3361 CAAAACTGGA TAGCTCTCCA GTGCTCTCCC CTGGGAACAA AGCCAAGCCT GAAGACCACC 
     3421 GCTCAAGGCC AGGCCGGCCC GCAGACTTTG TGTTGCTCAA AGAGCGGACT CTGGATGAGG 
     3481 CCCCTAAGCC TCCCAAGAAG GCCATGGACT ACTCCTCATC CAGTGAGGAG GTGGAAAGCA 
     3541 GTGAAGAGGA GGAGGAGGAA GGCGATGGGG AGCCGTCAGA GGGGAGCAGA GACACTCCCG 
     3601 GGGGCCGCAG TGATGGTGAT ACAGACAGCG TCAGCACCAT GGTGGTTCAT GATGTTGAGG 
     3661 AGATATCCGG GACCCAGCCC TCATATGGCG GCGGCACCAT GGTGGTCCAG CGTACTCCTG 
     3721 AAGAGGAACG AAGCCTGCTG CTTGCTGATA GCAATGGCTA CACAAACCTG CCTGATGTGG 
     3781 TCCAGCCCAG CCACTCACCT ACTGAGAACA GCAAAGGTCA AAGCCCTCCA ACAAAGGATG 
     3841 GAGGCAGTGA TTACCAGTCT CGTGGGCTGG TAAAGGCCCC AGGAAAGAGC TCATTCACCA 
     3901 TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGACACCATC CCTATCACAG 
     3961 CCCTAGTGGG TGGAGAAGGT GGTCGCCTTG ATCAACTGCA GTTCGATGTG AGGAAGGGCT 
     4021 CTGTGGTCAA CGTCAATCCC ACCAACACCC GAGCTCATAG TGAAACTCCT GAAATTCGCA 
     4081 AGTACAAGAA GCGATTCAAC TCAGAGATCC TATGTGCAGC TCTCTGGGGG GTCAACCTCC 
     4141 TAGTGGGCAC AGAGAATGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC AAGGTGTATG 
     4201 GACTTATTGG GCGACGACGC TTCCAGCAAA TGGATGTCTT AGAAGGGCTC AACTTGCTCA 
     4261 TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTATATTA CCTGTCCTGG CTTCGGAACA 
     4321 AGATCCTACA CAATGACCCA GAGGTGGAAA AGAAGCAGGG GTGGACCACC GTGGGGGACA 
     4381 TGGAGGGCTG CGGCCACTAC CGTGTTGTGA AATATGAACG GATTAAGTTC CTGGTCATTG 
     4441 CCCTGAAGAA CTCCGTGGAG GTTTATGCCT GGGCTCCCAA ACCCTACCAC AAATTCATGG 
     4501 CCTTCAAGTC CTTTGCTGAC CTCCCTCACC GCCCTCTACT GGTGGACCTG ACAGTAGAGG 
     4561 AGGGACAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT GTGGATGTTG 
     4621 ATTCTGGGAA CAGCTATGAC ATCTACATCC CTGTACATAT CCAGAGCCAG ATCACACCCC 
     4681 ACGCCATCAT CTTCCTCCCC AACACTGATG GCATGGAGAT GCTGCTGTGC TATGAAGATG 
     4741 AGGGTGTCTA TGTCAACACT TACGGGCGGA TCATCAAGGA TGTGGTGCTG CAGTGGGGAG 
     4801 AGATGCCCAC CTCTGTGGCC TACATCTGCT CCAACCAGAT AATGGGCTGG GGTGAGAAGG 
     4861 CCATAGAGAT CCGCTCTGTG GAGACAGGCC ACCTAGATGG GGTCTTCATG CACAAACGAG 
     4921 CCCAGAGGCT CAAGTTCCTG TGTGAGCGCA ATGACAAGGT GTTTTTTGCC TCTGTCCGCT 
     4981 CTGGAGGAAG CAGCCAAGTT TACTTTATGA CTCTGAACCG TAACTGCATC ATGAACTGGA 
     5041 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5101 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5161 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5221 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5281 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5341 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5401 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5461 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5521 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5581 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5641 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5701 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5761 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5821 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5881 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5941 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6001 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6061 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6121 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6181 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6241 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6301 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6361 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6421 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6481 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6541 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6601 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6661 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6721 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6781 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6841 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6901 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6961 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7021 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7081 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7141 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7201 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7261 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7321 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7381 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7441 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7501 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7561 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7621 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7681 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7741 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7801 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7861 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7921 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7981 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8041 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8101 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8161 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8281 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8341 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8401 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8881 AACGCGAATT TT
//